scholarly journals Prostaglandin F2? as ovulatory stimulus in dairy cows and buffaloes raised in the Amazon biome

2021 ◽  
Vol 42 (1) ◽  
pp. 193-208
Author(s):  
Luiz Francisco Pfeifer ◽  
◽  
Natália Castro ◽  
Jamyle Pereira ◽  
Augusto Schneider ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Single Point ◽  
Estradiol Benzoate ◽  
Preovulatory Follicle ◽  
Chi Square ◽  
Lactating Dairy Cows ◽  
Chi Square Test ◽  
The Moment ◽  
Dichotomous Outcomes ◽  
To Receive

This study evaluated the effect of a prostaglandin F2? (PGF) analogue as an ovulatory stimulus in dairy cows and buffaloes raised in the Amazon biome. To this end, three experiments were performed in the state of Rondônia, located in the Amazon biome. In Experiment 1, 22 lactating dairy buffaloes received 2 mg of intramuscular (I.M.) estradiol benzoate (EB) on day 0 and an intravaginal progesterone-releasing device (CIDR) from day 0 to day 9 of the protocol. On days 8 and 9, all cows were given 500 ?g of I.M. d-cloprostenol (PGF analogue). On day 10, buffaloes were divided into two groups to receive 500 ?g of PGF (PGF group, n = 8) or no treatment (CTL group, n = 14), respectively. In Experiment 2, 16 lactating crossbred dairy cows (Holstein x Gir) received 2 mg of EB on day 0 and a CIDR insert from day 0 to day 8. On days 7 and 8, all cows were given 500 ?g of d-cloprostenol. On day 9, cows were divided into two groups to receive 500 ?g of d-cloprostenol (PGF group, n = 8) or no treatment (CTL group, n = 8), respectively. In Experiment 3, 16 lactating crossbred dairy cows (Holstein x Gir) were handled and treated similarly as in Experiment 2, although cows did not receive d-cloprostenol on day 8. Single-point outcome variables were analyzed using one-way analysis of variance (ANOVA), while proportions with dichotomous outcomes were analyzed with the chi-square test. In Experiment 1, there was no difference (P = 0.30) in the ovulation rate between groups, and, on average, 68% of the buffaloes ovulated. Moreover, the treatment did not affect the interval to ovulation (P = 0.61) nor the diameter of the preovulatory follicle (P = 0.47). As for Experiment 2, only one cow, from the PG group, did not ovulate. There were no differences between the CTL and PG groups (P = 0.69) in the moment of ovulation, which occurred in average 82 h after CIDR removal. Finally, in Experiment 3, cows treated with PGF ovulated earlier than those in the CTL group (62.5 ± 5.8 and 94.5 ± 13.5 h, respectively; P = 0.05). Collectively, these results suggest that PGF hastens ovulation in lactating dairy cows, whereas no effect was observed in dairy buffaloes.

scholarly journals Addition of Clinoptilolite In The Diet Reduces Uterine PMN Leukocytes And Open Days In Multiparous Lactating Dairy Cows Managed In A Mountain Tropical Pasture-Based System

2021 ◽  
Author(s):  
Franklin I Sinchi ◽  
Jenny F. Zuin ◽  
Juan Pablo Garzón ◽  
Gonzalo E. López ◽  
Guido Calle ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Reproductive Performance ◽  
General Linear Model ◽  
Tropical Pasture ◽  
Chi Square ◽  
Lactating Dairy Cows ◽  
Uterine Health ◽  
Pmn Leukocytes ◽  
Chi Square Test ◽  
Subclinical Endometritis

Abstract This study was aimed to assess the effect of adding clinoptilolite in the diet on uterine health and reproductive performance in multiparous lactating dairy cows managed in a tropical pasture-based system above 2500 meters of altitude. Seventy-seven multiparous Holstein crossbred cows were allocated randomly into two groups: clinoptilolite supplemented cows (CLG, n = 42) and non-supplemented cows as control (CG, n = 35). Cows from CLG were supplemented with clinoptilolite from 30 days (50 g/cow/day) before to 60 days after calving (200 g/cow/day). Data were analyzed by general linear model, least squares means and chi-square test of SAS. In CLG cows, percentages of uterine PMN leukocytes (P<0.0001) and proportion of subclinical endometritis (P=0.0187) were lower than in CG. The interval calving to first corpus luteum was shorter (P=0.0759) in CLG than CG, and calving to first service interval was similar between treatments. Cows from CLG became pregnant 35 days earlier than CG cows (P=0.0224). In conclusion, daily addition of clinoptilolite in the diet decreased the proportion of cows with subclinical endometritis and shortened the interval from calving to conception in lactating dairy cows.

283 INFLUENCE OF THE MOMENT OF EMBRYO TRANSFER IN PREGNANCY RATES IN FEMALE BOVINE INSEMINATED WITH IVP EMBRYOS OFTHE NELLORE BREED

2010 ◽  
Vol 22 (1) ◽  
pp. 298
Author(s):  
A. C. B. Mariani ◽  
J. F. Souza ◽  
F. B. Lopes ◽  
J. A. T. Souza ◽  
J. L. Ferreira ◽  
...  
Keyword(s):  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Fertilization In Vitro ◽  
In Vitro Production ◽  
Chi Square ◽  
Significance Level ◽  
Animal Reproduction ◽  
Chi Square Test ◽  
The Moment

The in vitro production (IVP) of bovine embryos is a biotechnique that has created new perspectives in animal reproduction and improvement. However, one of the main issues in the diffusion of this technology concerns the rate of pregnancy of the recipient cows. The objective was to verify the relationship between the fertilization in vitro, the day the recipient female was in estrus, and the pregnancy rates. Two hundred sixty crossbred recipient cows were selected from July to November 2008, synchronized with 2 different protocols at a random moment of the estrous cycle. One of the groups (G1) composed of 110 females that received 2.0 mL of D-cloprostenol, i.m. in only dose. The other group (G2), with 150 females, received a norgestomet auricular implant and 2.0 mg of estradiol benzoate i.m. On the fifth day, the females received, i.m. 200 IU of equine serum gonadotrophin and 150 μg of D-cloprostenol. The implant was removed on the eighth day, and 24 h later all animals received 1.0 mg of estradiol benzoate. The recipients entered in estrus 1 day before, 1 day after, and on the day of IVF. The embryos were transferred on Day 7 after fertilization, trancervically in the ipsilateral uterine horn that contained corpus luteum. The gestation diagnosis was made by ultrasound scan 30 days after the transfer. Data were analyzed using SAS software (version 9.0, SAS Institute, Cary, NC, USA). Initially, data were analyzed by normality test using Proc Univariate; however, a normal distribution was not observed. Data were then analyzed by PROC FREQ with nonparametric method of chi-square test and a significance level of 5%. Among the recipients, 40 were observed in estrus 1 day before IVF (D - 1), 137 on the IVF day (D0) and 83 were observed in estrus 1 day after (D + 1), resulting in a total rate of pregnancy of 38.8%. For each day the resulting pregnancy rates were 42.50% in D - 1, 41.61% in D - 0, and 30.12% in D + 1. These results suggest that there is no relationship between the day of the recipient’s estrus, the day of IVF, and pregnancy rates. There was no influence of the day of estrus in relation to the day of IVF in the gestation rates of the recipients of IVP embryos. However, more studies concerning the recipients are necessary, because they assume an important role in the success of the IVP. The authors thank Brio Genetic and Technology.

Hubungan Antara Menyusui Dengan Involusi Uterus Pada Ibu Nifas Fisiologis Di RSIA Aura Syifa Kabupaten Kediri

1970 ◽  
Vol 5 (2) ◽  
pp. 64
Author(s):  
Eny Sendra ◽  
Dewi Indriani
Keyword(s):  
Breast Feeding ◽  
Sampling Technique ◽  
Chi Square ◽  
Cross Sectional ◽  
Fisher Test ◽  
Key Word ◽  
Chi Square Test ◽  
The Moment

Breast feeding is giving milk to be drunk to the baby from the breast. Uterus involution is a process how the uterus return to the condition back, before pregnanting after bearing. At the moment of suckling, happens a stimulus and brings the hormones out, such as oksitosin uses not only to stimulate some muscles constraction but also to stimulate the uterus, so that the process of uterus involution happens foster. According to the explanations above, the research aimed to know about the correlation between breast feeding and uterus involution. This research’s design was, cross sectional by the population of all childbirth mothers approximately 50 persons / month. By using accidental sampling technique we got 21 sample respondents. The place of research in RSIA Aura Syifa in Kediri Regency on 16th until 22nd of June 2009. From this research’s result, we got 14 persons (66,67%) with normal uterus involution, suckled in a good way, one person (4,67%) with normal uterus involution, suckled in a wrong way, 2 persons (9,52%) with abnormal uterus involution, suckled in a good way and 4 persons (19,05%) with abnormal uterus involution, suckled in a wrong way. Statistic test which used chi-square test, counted the probability frequency in advance, from that we got 3 columns with the score, less than 5, so that chi-square can not be continued and by doing exact fisher test, the score was 0,001. Because P with the grade mistake 0,05 smaller, so the conclusion was “Ho” is rejected, it meant “there was correlation between suckling and uterus involution”. Key Word : Breast feeding, uterus involution

scholarly journals Comparison of blood plasma and gelatin solution in resuscitation of children with dengue shock syndrome

2009 ◽  
Vol 49 (6) ◽  
pp. 322
Author(s):  
Suryadi Nicolaas Napoleon Tatura ◽  
Novie Homenta Rampengan ◽  
Jose Meky Mandei ◽  
Ari Lukas Runtunuwu ◽  
Max FJ Mantik ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Dengue Shock Syndrome ◽  
Shock Rate ◽  
Gelatin Solution ◽  
Chi Square ◽  
Management Guidelines ◽  
Exact Test ◽  
Chi Square Test ◽  
A Prospective Study ◽  
To Receive

Background Dengue shock syndrome (DSS) is characterized bysevere vascular leakage and hemostasis disorder. It is the cause of death in 1 to 5 percent of cases. WH 0 management guidelines for resuscitation remain empirical rather than evidence-based.Objective To find out the alternative fluids to replace plasmaleakage in DSS.Methods We performed a prospective study and randomizedcomparison of plasma and gelatin solution for resuscitation ofIndonesian children with DSS. We randomly assigned 25 subjectswith DSS to receive plasma and 25 children to receive gelatinfluid. Statistical analyse were performed using chi-square test,Fisher's exact test, t test, Mann-Whitney test.Results The increment of pulse pressure width and the decrement of hematocrit in subjects treated with gelatin were higher than that of plasma atfour-hour therapy (P=0.002 and P=0.017). Only one patient died caused by unusually manifestation of DSS. The increment of body temperature in subjects treated with plasma was higher than that of gelatin at four-hour therapy (P=O.Oll). The decrement of platelet count in subjects treated with gelatin were less than that of plasma (P=0.018). The increment of diuresis rate in subjects treated with gelatin was higher than that of plasma at twenty-hour therapy (P<O.OOOl). The decrement of respiratory rate in subjects treated with gelatin was higher than that of plasmaat twenty-eight hour therapy (P=0.018). There was no differencein studied variables : total volume rate, blood pressure, pulse rate, re-shock rate, clinical fluid overload, allergy reactions, bleeding manifestations, and length of stay (P>0.05).Conclusions Gelatin solution can be used as volume replacementin resuscitation of DSS if blood plasma is not available especiallyat four-hour therapy.

scholarly journals The Effects of Administering Estradiol Benzoate Plus Progesterone During the Growth or Static Phases of the Dominant Follicle in CIDR-Treated Lactating Dairy Cows

2007 ◽  
Vol 53 (3) ◽  
pp. 591-596 ◽  
Author(s):  
Ui-Hyung KIM ◽  
Guk-Hyun SUH ◽  
Tai-Young HUR ◽  
Seog-Jin KANG ◽  
Hyun-Gu KANG ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Estradiol Benzoate ◽  
Dominant Follicle ◽  
Lactating Dairy Cows

23 EFFECTS OF THE NUMBER OF SERVICES FERTILITY IN DAIRY COWS

2011 ◽  
Vol 23 (1) ◽  
pp. 118 ◽  
Author(s):  
M. Yamaguchi ◽  
M. Tanisawa ◽  
H. Koyama ◽  
S. Takahashi ◽  
O. Dochi
Keyword(s):  
Dairy Cows ◽  
Conception Rate ◽  
Low Fertility ◽  
Chi Square ◽  
The Third ◽  
Total Percentage ◽  
Conception Rates ◽  
Service Conception ◽  
Chi Square Test ◽  
Rectal Palpation

In recent years, reproductive performance of dairy cows has been declining worldwide, especially among cows of high genetic merit for milk production. The cause of the low fertility may considerably vary across countries and is probably multifactorial. This problem remains unsolved. Further, the first-service conception rate of dairy cows has remarkably decreased worldwide. The number of services required for conception has increased due to the low fertility in dairy cows. However, there are few reports about the relationship between conception rates and the number of services in the current dairy cows. The objective of this study was to investigate whether the number of services affects the conception rates of dairy cows. Data concerning the conception rates was obtained for 8386 Holstein cows from 40 commercial dairy herds in eastern Hokkaido, Japan, from 2006 to 2009. The diagnosis of pregnancy was confirmed by rectal palpation between 30 and 45 days after insemination. The average interval between calving and the first-service was 87.3 days. The average milk yield was 8500 kg. Number of conducting services was from the first to the seventh service. The conception rate was analysed using chi-square test. The results are presented in Table1. The conception rate from the first to the seventh service was 40.2 to 54.0%. The conception rate at the first service was significantly low (40.2%); however, the conception rates did not significantly differ after the second-service. A similar tendency was observed during each year. Moreover, the total percentage of conceptions from the first to the third service was 83.8%. The average number of services per conception was 2.2. The results of this study indicate that the first service yielded the lowest conception rates. The conception rates after the second service did not significantly differ. Moreover, ∼84% of the dairy cows became pregnant between the first service and the third service. However, this result shows that ∼16% of the dairy cows in the herds were repeat breeding. In order to improve fertility, it is necessary to study the factors that affect the first-service conception rates of dairy cows. Moreover, to improve the conception rate of dairy cows, it is important to elucidate the cause of these problems. Table 1.The number and percentage of dairy cows conceiving at each of the services for consecutive services

Pathomorphometry of ruptured intracranial vertebral arterial dissection: adventitial rupture, dilated lesion, intimal tear, and medial defect

2013 ◽  
Vol 119 (1) ◽  
pp. 221-227 ◽  
Author(s):  
Ayako Ro ◽  
Norimasa Kageyama
Keyword(s):  
Cross Sections ◽  
Single Point ◽  
Vascular Lesions ◽  
Internal Elastic Lamina ◽  
Chi Square ◽  
Site Distribution ◽  
Chi Square Test ◽  
Life Threatening ◽  
Vertebrobasilar Junction ◽  
Elastic Lamina

Object Subarachnoid hemorrhage (SAH) due to ruptured intracranial vertebral artery (VA) dissection is a life-threatening disease. Angiographic and symptomatic prognostic factors for rupture and rerupture have been investigated, but the pathological characteristics have not been fully investigated. The authors aimed to investigate these features by performing a pathomorphometic study of ruptured intracranial VA dissections. Methods This study included 50 administrative autopsy cases of fatal SAH due to ruptured intracranial VA dissection among 517 fatal nontraumatic cases of SAH occurring between March 2003 and May 2011. Pathomorphometry was performed using serial 5-μm histological cross-sections with elastica van Gieson staining from each 0.2-mm segment around the ruptured intracranial VA. The longitudinal lengths of 4 types of vascular lesions—adventitial ruptures, dilated lesions where the internal elastic lamina (IEL) was ruptured with adventitial extension, intimal tears where the IEL was ruptured, and medial defects—were calculated based on the numbers of the slides in which these lesions were continuously detected (minimum 2 adjoining slides). The distance from the vertebrobasilar junction to the center of adventitial rupture was also calculated in 37 cases. Results All cases showed one adventitial rupture with a mean length of about 1.9 ± 1.1 mm. The center of the adventitial rupture was located 5.0–26.8 mm (mean 14.6 ± 5.5 mm) from the intracranial VA bifurcation. Adventitial ruptures existed in the centers of dilated lesions, where the adventitia was highly extended. Other vascular lesions were serially observed surrounding the adventitial rupture. The mean lengths of dilated lesions, intimal tears, and medial defects were 9.4 ± 4.8 mm, 13.2 ± 6.3 mm, and 15.6 ± 7.2 mm, respectively. The lengths between proximal lesions and distal lesions from the center of the adventitial rupture for both medial defects and intimal tears were significantly longer at proximal lesions than at distal ones (chi-square test, p < 0.01). Conclusions Every ruptured intracranial VA dissection has a single point of adventitial rupture where the adventitia was maximally extended, so dilation appears to be a valuable predictive factor for hemorrhagic intracranial VA dissections. The adventitial ruptures were as small as 2 mm in length, and clinically detectable dilated lesions were about 9 mm in length. However, vascular vulnerability caused by IEL ruptures and medial defects existed more widely across a length of VA of 1.3–1.5 cm. Comparatively broader protection of the intracranial VA than the clinically detected area of dissection might be desirable to prevent rebleeding. Broader protection of proximal lesions than distal lesions might be effective from the viewpoint of site distribution of vascular lesions and blood flow alteration to the pseudolumen caused by the dissecting hematoma. Medial defects are the most widely seen lesions among the 4 types of vascular lesions studied. Medial degenerative disease, known as segmental arterial mediolysis, is suspected in the pathogenesis of intracranial VA dissections.

scholarly journals Evaluation of knowledge and application towards ergonomic principles among undergraduate dental students

2021 ◽  
Vol 10 (14) ◽  
pp. e123101421561
Author(s):  
Marcely Silva Araújo ◽  
Vandilson Pinheiro Rodrigues ◽  
Rogério Vera Cruz Ferro Marques ◽  
Andre Luis Costa Cantanhede ◽  
Isabelle Aguiar Prado ◽  
...  
Keyword(s):  
Dental Students ◽  
The Body ◽  
Descriptive Statistics ◽  
Categorical Variables ◽  
Chi Square ◽  
Experienced Pain ◽  
Chi Square Test ◽  
Dental Setting ◽  
The Moment ◽  
The Relationship

This study evaluated the knowledge and application of ergonomic principles as well as manifestation of pain by dental students at the Federal University of Maranhão. Forty-four students answered a questionnaire on ergonomic principles. Photographs of the clinical rounds were performed to evaluate the postures adopted, without the students knowing the moment of photographic registration, avoiding that they modified their posture. The relationship between theoretical knowledge and the applicability of ergonomics in dental setting was evaluated. Data were analyzed using descriptive statistics. The Chi-square test analyzed the distribution of categorical variables among the evaluation instruments (α = 5%). Most of the participants adopted a satisfactory posture and presented knowledge about ergonomic postures, however, one can observe the poor positioning of the eyes and spine. 75% of the patients experienced pain in the body, being the spine (61.4%) and neck (40.9%) the most affected sites. The sample evaluated had good knowledge on ergonomic principles, but these were not adopted entirety, which may have led to the onset of pain.                

10 EFFECT OF USING PROTECTIVE AI COVER SHEATHS ON FERTILITY OF LACTATING DAIRY COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 163
Author(s):  
S. Bas ◽  
A. Hoet ◽  
P. Rajala-Schultz ◽  
D. Sanders ◽  
G. M. Schuenemann
Keyword(s):  
Dairy Cattle ◽  
Dairy Cows ◽  
Dairy Farm ◽  
Cost Effective ◽  
Reproductive Outcomes ◽  
Lactating Cows ◽  
Lactating Dairy Cows ◽  
Herd Milk ◽  
Underlying Mechanisms ◽  
The Moment

An adequate and clean artificial insemination (AI) technique is recommended to maximize reproductive outcomes in dairy cattle. The objective of this study was to evaluate the effectiveness of using protective sheaths (PS; Continental Plastic Inc., Delavan, WI, USA) to minimize contamination of the AI catheter (AIC) on pregnancies per AI (P/AI) in lactating dairy cattle. A previous study reported no improvement on cattle fertility when using PS during first service AI (King et al. 1984 Can. Vet. J. 25, 327). Lactating cows housed in free-stall barns on a commercial dairy farm with a rolling herd milk production average of 10.140 kg were presynchronized with 2 injections of PGF2α (25 mg; Lutalyse, Pharmacia & Upjohn, Kalamazoo, MI, USA) given 14 days apart (starting at 26 ± 3 d postpartum) followed by Ovsynch [OV; GnRH-7 d-PGF2α-56 h-GnRH-16 h-timed-AI(TAI)] 12 days later. Cows presenting signs of standing heat any time during the protocol received AI, whereas the remaining animals were subjected to TAI16 h after second OV GnRH (100 μg; Cystorelin, Merial, Duluth, GA, USA). At the moment of AI (one AI technician), 996 services from lactating dairy cows were randomly assigned to 1 of the 2 groups; with (TRT, n = 487) or without (CON, n = 509) the use of PS. In the TRT group, the AIC protected with a PS was introduced into the vagina; once in the cranial portion of the vagina adjacent to the cervical os, the PS was pulled back and only the AIC was manipulated through the cervix into the uterine body for semen deposition. In the CON group, cows received AI without the PS. Additionally, sterile cotton swab (BD, Franklin Lakes, NJ, USA) samples were taken from AIC (n = 51) after AI from both treatment groups. Pregnancy diagnosis was determined by ultrasonography 42 ± 3 d after AI. Data analyses were performed using GLIMMIX (P/AI) and FREQ (culture) procedures of SAS (SAS Institute Inc., Cary, NC, USA). Cultured swab samples revealed that the use of PS was effective in minimizing contamination of the AIC (bacterial growth on AIC; TRT = 57.7% v. CON = 100%; P < 0.0002). Overall, the proportion of cows pregnant (all services) was greater (P = 0.03) for cows in TRT (42.7 ± 2.2%) compared with CON group (36.1 ± 2.1%). For first services postpartum, P/AI did not differ (P = 0.87) between CON (43.01 ± 4.4%) and TRT (43.8 ± 4.6%) groups. However, P/AI for second or greater services (≥2S) were greater (P = 0.007) in TRT (43.8 ± 2.9%) than in CON cows (32.3 ± 2.6%). Results from this study suggested that the use of PS during AI improved P/AI for ≥2S in lactating dairy cows. Performing a clean AI technique through the use of PS may be a cost-effective strategy to improve reproductive outcomes in dairy cattle. Further investigation is needed under various reproductive management conditions to confirm and determine the underlying mechanisms for these findings. Authors thank Coba/Select Sires Inc. (Columbus, OH, USA) for the donation of the AI protector sheaths, Meerland Dairy for providing the animals and Brian Alkire (Coba’s AI technician).

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