Chickpea seed vigor evaluated by computerized seedling analysis

Computerized seedling analysis is a promising tool for assessing seed vigor, providing reliable and less subjective results. The aim of this study was to define a method for evaluating the vigor of chickpea seeds through computerized image analysis, using the Seedling Analysis System (Sistema de Análise de Plântula - SAPL®) as a basis. Two chickpea cultivars were evaluated, consisting of five seed lots of cv. BRS Aleppo and four seed lots of cv. Cícero. Test were performed on the seeds of each cultivar for initial characterization of the physiological potential of the lots, and the results were compared with the determinations made via SAPL®. The results showed high correlations between the variables obtained from computerized analysis and from traditional vigor tests, especially for the close relationship between the cold test and low temperature germination test with the corrected vigor index (r > 0.9) evaluated on the fourth day after the beginning of the test. The total length of seedlings, length of the primary root, and growth and corrected vigor indices obtained through computerized analysis of seedlings on the 4th day after sowing allowed separation of the lots regarding vigor. Computerized analysis of seedlings using SAPL® is effective in determining the vigor of chickpea seed lots.

Download Full-text

Semi-automated Phenotyping of Soybean Seedlings and Its Relation With Physiological Seed Quality

Journal of Agricultural Science ◽

10.5539/jas.v11n16p1 ◽

2019 ◽

Vol 11 (16) ◽

pp. 1

Author(s):

Jackson Araújo da Silva ◽

André Dantas de Medeiros ◽

Daniel Teixeira Pinheiro ◽

Marcio Dias Pereira ◽

Laércio Junio da Silva ◽

...

Keyword(s):

Seed Quality ◽

Seedling Emergence ◽

Primary Root ◽

Soybean Seed ◽

Cold Test ◽

Research Field ◽

Seed Vigor ◽

Promising Alternative ◽

Soybean Seedlings ◽

Vigor Index

The development of procedures enabling agility and effectiveness to the analyses of seed vigor are great advances for the seed research field. The aim of this paper was to evaluate the efficiency of the Seedling Analysis System (SAPL®) to seedling phenotyping and determining the physiological potential of soybean seeds, in comparison with the information provided by traditional vigor tests recommended for this species. The characterizing of physiological potential of the seed lots was carried out based on germination tests, first germination count, seedling emergence, cold test, and electrical conductivity. The results of these tests were compared with the data obtained using image analysis with the software SAPL®, which was used to evaluate the development seven- day old seedlings. It was obtained the length of shoot, primary root and entire seedling, growth rates, uniformity, vigor index and corrected vigor index, based on different variations of the growth/uniformity ratio, namely 80:20, 70:30, 60:40, in the vigor index calculation. Analysis of images of the seven-day old seedlings using SAPL® enables phenotyping of soybean seedlings and is a consistent and promising alternative for identifying differences in vigor between soybean seed lots, equivalent to that found with traditional vigor tests.

Download Full-text

SAPL®: a free software for determining the physiological potential in soybean seeds1

Pesquisa Agropecuária Tropical ◽

10.1590/1983-40632018v4852340 ◽

2018 ◽

Vol 48 (3) ◽

pp. 222-228 ◽

Cited By ~ 6

Author(s):

André Dantas de Medeiros ◽

Márcio Dias Pereira

Keyword(s):

Seedling Emergence ◽

Soybean Seed ◽

Cold Test ◽

Seed Vigor ◽

Soybean Seeds ◽

Germination Test ◽

Vigor Index ◽

Seedling Length ◽

Powerful Approach ◽

Analysis System

ABSTRACT Obtaining image-based information is a powerful approach to capture and quantify seed vigor data. However, commercial systems that facilitate the processing and acquisition of images are often cost prohibitive. This study aimed to evaluate the efficiency of the Seedling Analysis System (Sistema de Análise de Plântulas - SAPL®), in order to analyze the physiological potential of soybean seeds, in comparison with the information provided by vigor tests which are traditionally recommended for this species. Nine lots of soybean seeds were submitted to germination, germination speed, seedling emergence, cold test and electrical conductivity tests. In the SAPL® analyzes, seedlings of four and six days, counted from the beginning of the germination test, were evaluated, resulting in values for seedling length, growth, development uniformity, vigor index and corrected vigor index. The evaluated lots with emergence greater than 90 % showed a vigor index higher than 600 and 800, respectively in the fourth and sixth days. The indexes generated by SAPL®, except for the uniformity index, presented positive and high correlations with the traditional tests (> 0.80). SAPL® is efficient in identifying differences in the vigor of soybean seed lots.

Download Full-text

Assessment of Seed Vigor Tests for Crambe abyssinica

Journal of Agricultural Science ◽

10.5539/jas.v10n12p527 ◽

2018 ◽

Vol 10 (12) ◽

pp. 527

Author(s):

Tássia Fernanda Santos Neri Soares ◽

Bruno Antônio Lemos de Freitas ◽

José Geraldo de Araújo Ferreira Filho ◽

Denise Cunha Fernandes dos Santos Dias ◽

Camila Andrade Fialho ◽

...

Keyword(s):

Electrical Conductivity ◽

Water Stress ◽

Stress Test ◽

Primary Root ◽

Cold Test ◽

Seed Vigor ◽

Stress Tests ◽

Germination Test ◽

Crambe Abyssinica ◽

Seedling Length

Vigor tests are important mainly to identify differences associated to lots performance in field conditions. The objective of this study was to define the most appropriate tests to evaluate the crambe (Crambe abyssinica) seeds vigor. Seven seed lots of crambe were subjected to the standard germination (on the first and last count), germination speed index (GSI), primary root protrusion speed index (PRPSI), seedling length dry and weight seedling, water stress test using PEG 6000 at -0.2 MPa, electrical conductivity and cold test without soil. Crambe lots studied had germination percentages varying from 54 to 80.5%. The GSI, on the first count of water stress and electrical conductivity test ranked lots between two vigor classes The PRPSI, seedling length, seedling dry weight and last count of water stress tests were less sensitive to evaluate vigor for crambe seed. The cold test without soil and the first count of standard germination test were capable to dishtinguish lots more efficiently than the standard germination test on last count by ranking four of the seven seed lots studied.

Download Full-text

Physiological and antioxidant changes in sunflower seeds under water restriction

Journal of Seed Science ◽

10.1590/2317-1545v42225777 ◽

2020 ◽

Vol 42 ◽

Author(s):

Thais de Castro Morais ◽

Daniel Teixeira Pinheiro ◽

Paola Andrea Hormaza Martinez ◽

Fernando Luiz Finger ◽

Denise Cunha Fernandes dos Santos Dias

Keyword(s):

Antioxidant Enzymes ◽

Primary Root ◽

Field Performance ◽

Seed Vigor ◽

Sunflower Seeds ◽

Water Restriction ◽

Sod Activity ◽

Initial Characterization ◽

Lower Capacity ◽

Primary Root Length

Abstract: Seed vigor may be determinant for field performance, especially under water restriction conditions. Sunflower is a crop subject to these conditions in the field and, therefore, the aim of this study was to evaluate the physiological and antioxidant changes in sunflower seeds under water restriction. Two lots of sunflower seeds (cv. Hélio 253) with different vigor levels were used. After initial characterization, seeds were placed to germinate under water potentials of 0.0, -0.2, -0.4, -0.6 and -0.8 MPa and evaluated by tests of germination, first germination count, shoot length and primary root length. The activities of the antioxidant enzymes SOD, CAT, POX and APX were also evaluated at 0, 2, 4 and 6 days after sowing. Water restriction led to a decrease in germination and slower seedling growth, regardless of seed vigor level. SOD activity was similar in the two lots, with reduction in activity four days after sowing. CAT activity was affected differently during germination in the two lots, and it was generally higher in the most vigorous lot. In higher vigor seeds, there was lower POX activity in water restriction treatments compared to the control. In general, seeds of lower vigor have lower capacity for activation of antioxidant enzymes, especially peroxidases.

Download Full-text

Assessment of the physiological potential of super sweet corn seeds

Journal of Seed Science ◽

10.1590/s2317-15372013000300010 ◽

2013 ◽

Vol 35 (3) ◽

pp. 340-346 ◽

Cited By ~ 3

Author(s):

Renata Oliveira Alvarenga ◽

Julio Marcos-Filho ◽

Tathiana Silva Timóteo

Keyword(s):

Seed Quality ◽

Sweet Corn ◽

Seedling Emergence ◽

Primary Root ◽

Accelerated Aging ◽

Cold Test ◽

Seed Vigor ◽

Corn Seed ◽

Control Programs ◽

Seedling Length

The assessment of physiological potential is essential in seed quality control programs. This study compared the sensitivity of different procedures for evaluating super sweet corn seed vigor, focusing on the primary root protrusion test. Six seed lots, each of the SWB 551 and SWB 585 hybrids, were used. Seed physiological potential was evaluated by germination and vigor tests (speed of germination, traditional and saturated salt accelerated aging, cold test, seedling length, seedling emergence and primary root protrusion). Primary root protrusion was evaluated every 12 hours at 15 °C, 20 °C and 25 °C using two criteria (primary root protrusion and seedlings at the 2 mm root stage). It was concluded that the primary root protrusion test at 15 °C can evaluate super sweet corn seed vigor by counting the number of seedlings at the 2 mm root stage.

Download Full-text