scholarly journals SAPL®: a free software for determining the physiological potential in soybean seeds1

2018 ◽  
Vol 48 (3) ◽  
pp. 222-228 ◽  
Author(s):  
André Dantas de Medeiros ◽  
Márcio Dias Pereira
Keyword(s):  
Seedling Emergence ◽  
Soybean Seed ◽  
Cold Test ◽  
Seed Vigor ◽  
Soybean Seeds ◽  
Germination Test ◽  
Vigor Index ◽  
Seedling Length ◽  
Powerful Approach ◽  
Analysis System

ABSTRACT Obtaining image-based information is a powerful approach to capture and quantify seed vigor data. However, commercial systems that facilitate the processing and acquisition of images are often cost prohibitive. This study aimed to evaluate the efficiency of the Seedling Analysis System (Sistema de Análise de Plântulas - SAPL®), in order to analyze the physiological potential of soybean seeds, in comparison with the information provided by vigor tests which are traditionally recommended for this species. Nine lots of soybean seeds were submitted to germination, germination speed, seedling emergence, cold test and electrical conductivity tests. In the SAPL® analyzes, seedlings of four and six days, counted from the beginning of the germination test, were evaluated, resulting in values for seedling length, growth, development uniformity, vigor index and corrected vigor index. The evaluated lots with emergence greater than 90 % showed a vigor index higher than 600 and 800, respectively in the fourth and sixth days. The indexes generated by SAPL®, except for the uniformity index, presented positive and high correlations with the traditional tests (> 0.80). SAPL® is efficient in identifying differences in the vigor of soybean seed lots.

scholarly journals Semi-automated Phenotyping of Soybean Seedlings and Its Relation With Physiological Seed Quality

2019 ◽  
Vol 11 (16) ◽  
pp. 1
Author(s):  
Jackson Araújo da Silva ◽  
André Dantas de Medeiros ◽  
Daniel Teixeira Pinheiro ◽  
Marcio Dias Pereira ◽  
Laércio Junio da Silva ◽  
...  
Keyword(s):  
Seed Quality ◽  
Seedling Emergence ◽  
Primary Root ◽  
Soybean Seed ◽  
Cold Test ◽  
Research Field ◽  
Seed Vigor ◽  
Promising Alternative ◽  
Soybean Seedlings ◽  
Vigor Index

The development of procedures enabling agility and effectiveness to the analyses of seed vigor are great advances for the seed research field. The aim of this paper was to evaluate the efficiency of the Seedling Analysis System (SAPL®) to seedling phenotyping and determining the physiological potential of soybean seeds, in comparison with the information provided by traditional vigor tests recommended for this species. The characterizing of physiological potential of the seed lots was carried out based on germination tests, first germination count, seedling emergence, cold test, and electrical conductivity. The results of these tests were compared with the data obtained using image analysis with the software SAPL®, which was used to evaluate the development seven- day old seedlings. It was obtained the length of shoot, primary root and entire seedling, growth rates, uniformity, vigor index and corrected vigor index, based on different variations of the growth/uniformity ratio, namely 80:20, 70:30, 60:40, in the vigor index calculation. Analysis of images of the seven-day old seedlings using SAPL® enables phenotyping of soybean seedlings and is a consistent and promising alternative for identifying differences in vigor between soybean seed lots, equivalent to that found with traditional vigor tests.

scholarly journals Seedling imaging analysis and traditional tests to assess okra seed vigor

2013 ◽  
Vol 35 (4) ◽  
pp. 443-448 ◽  
Author(s):  
Ana Lúcia Pereira Kikuti ◽  
Júlio Marcos-Filho
Keyword(s):  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Seed Industry ◽  
Accelerated Aging Test ◽  
Vigor Index ◽  
Seedling Length ◽  
Germination Speed

Seed vigor testing is an important component of quality control programs adopted by seed industry. The software Seed Vigor Imaging System (SVIS) has been successfully used for seed vigor assessment in different species. The objective of this research was to verify the SVIS efficiency to assess okra seed vigor in comparison to other vigor tests used for this species. Five seed lots of 'Clemson Americano' and four of 'Santa Cruz' were submitted to germination (speed and percentage), cold germination (speed and percentage), traditional and saturated salt accelerated aging (41 ºC/72 and 96 h) and seedling emergence tests during 12 months storage. Vigor index, uniformity of growth and seedling length were determined by the software Seed Vigor Imaging System (SVIS). Results showed that the vigor index and seedling length determined by the SVIS analyses are efficient to determine okra seed vigor as well as the accelerated aging test (41 ºC/72 h).

scholarly journals Assessment of Seed Vigor Tests for Crambe abyssinica

2018 ◽  
Vol 10 (12) ◽  
pp. 527
Author(s):  
Tássia Fernanda Santos Neri Soares ◽  
Bruno Antônio Lemos de Freitas ◽  
José Geraldo de Araújo Ferreira Filho ◽  
Denise Cunha Fernandes dos Santos Dias ◽  
Camila Andrade Fialho ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Water Stress ◽  
Stress Test ◽  
Primary Root ◽  
Cold Test ◽  
Seed Vigor ◽  
Stress Tests ◽  
Germination Test ◽  
Crambe Abyssinica ◽  
Seedling Length

Vigor tests are important mainly to identify differences associated to lots performance in field conditions. The objective of this study was to define the most appropriate tests to evaluate the crambe (Crambe abyssinica) seeds vigor. Seven seed lots of crambe were subjected to the standard germination (on the first and last count), germination speed index (GSI), primary root protrusion speed index (PRPSI), seedling length dry and weight seedling, water stress test using PEG 6000 at -0.2 MPa, electrical conductivity and cold test without soil. Crambe lots studied had germination percentages varying from 54 to 80.5%. The GSI, on the first count of water stress and electrical conductivity test ranked lots between two vigor classes The PRPSI, seedling length, seedling dry weight and last count of water stress tests were less sensitive to evaluate vigor for crambe seed. The cold test without soil and the first count of standard germination test were capable to dishtinguish lots more efficiently than the standard germination test on last count by ranking four of the seven seed lots studied.

scholarly journals Chickpea seed vigor evaluated by computerized seedling analysis

2021 ◽  
Vol 42 (1) ◽  
pp. 71-86
Author(s):  
Joyce de Oliveira Araújo ◽  
◽  
Denise Cunha Fernandes dos Santos Dias ◽  
André Dantas de Medeiros ◽  
Laércio Junio da Silva ◽  
...  
Keyword(s):  
Primary Root ◽  
Cold Test ◽  
Seed Vigor ◽  
Computerized Analysis ◽  
Promising Tool ◽  
Vigor Index ◽  
Initial Characterization ◽  
Close Relationship ◽  
Analysis System

Computerized seedling analysis is a promising tool for assessing seed vigor, providing reliable and less subjective results. The aim of this study was to define a method for evaluating the vigor of chickpea seeds through computerized image analysis, using the Seedling Analysis System (Sistema de Análise de Plântula - SAPL®) as a basis. Two chickpea cultivars were evaluated, consisting of five seed lots of cv. BRS Aleppo and four seed lots of cv. Cícero. Test were performed on the seeds of each cultivar for initial characterization of the physiological potential of the lots, and the results were compared with the determinations made via SAPL®. The results showed high correlations between the variables obtained from computerized analysis and from traditional vigor tests, especially for the close relationship between the cold test and low temperature germination test with the corrected vigor index (r > 0.9) evaluated on the fourth day after the beginning of the test. The total length of seedlings, length of the primary root, and growth and corrected vigor indices obtained through computerized analysis of seedlings on the 4th day after sowing allowed separation of the lots regarding vigor. Computerized analysis of seedlings using SAPL® is effective in determining the vigor of chickpea seed lots.

scholarly journals Spray volumes in the industrial treatment on the physiological quality of soybean seeds with different levels of vigor

2017 ◽  
Vol 39 (2) ◽  
pp. 174-181 ◽  
Author(s):  
Cristian Rafael Brzezinski ◽  
Julia Abati ◽  
Fernando Augusto Henning ◽  
Ademir Assis Henning ◽  
José de Barros França Neto ◽  
...  
Keyword(s):  
Experimental Design ◽  
Bradyrhizobium Japonicum ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Seed Vigor ◽  
Soybean Seeds ◽  
Germination Test ◽  
Physiological Quality ◽  
Different Levels

Abstract: The aim of this work was to evaluate the effect of different spray volumes, via industrial treatment, on the physiological quality of soybean seeds with different levels of vigor. The experimental design was completely randomized in a 2x5 factor scheme, with four replications. Factors consisted in two levels of seed vigor (high and low) and five spray volumes (0, 600, 1200, 1800 and 2400 mL. 100 kg-1). Products used to obtain the volumes were: fungicide (carbendazin + thiram); insecticide (imidacloprid + thiodicarb); nematicide (abamectin); micronutrients (cobalt, molybdenum and zinc); polymer (peridiam); biostimulants (kinetin + gibberellic acid) and inoculum (Bradyrhizobium japonicum). The used cultivars were BRS 360 RR and BRS 1010 IPRO. The physiological seed quality was determined by the following evaluations: germination, first count of the germination test, seedling emergence in sand, emergency speed index, total length of shoot and root of the seedlings. In the different spray volumes, high vigor soybean seeds have higher physiological quality than low vigor seeds. The increased spray volumes in seed treatments reduce the physiological quality of low vigor soybean seeds.

scholarly journals Assessment of the physiological potential of super sweet corn seeds

2013 ◽  
Vol 35 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Renata Oliveira Alvarenga ◽  
Julio Marcos-Filho ◽  
Tathiana Silva Timóteo
Keyword(s):  
Seed Quality ◽  
Sweet Corn ◽  
Seedling Emergence ◽  
Primary Root ◽  
Accelerated Aging ◽  
Cold Test ◽  
Seed Vigor ◽  
Corn Seed ◽  
Control Programs ◽  
Seedling Length

The assessment of physiological potential is essential in seed quality control programs. This study compared the sensitivity of different procedures for evaluating super sweet corn seed vigor, focusing on the primary root protrusion test. Six seed lots, each of the SWB 551 and SWB 585 hybrids, were used. Seed physiological potential was evaluated by germination and vigor tests (speed of germination, traditional and saturated salt accelerated aging, cold test, seedling length, seedling emergence and primary root protrusion). Primary root protrusion was evaluated every 12 hours at 15 °C, 20 °C and 25 °C using two criteria (primary root protrusion and seedlings at the 2 mm root stage). It was concluded that the primary root protrusion test at 15 °C can evaluate super sweet corn seed vigor by counting the number of seedlings at the 2 mm root stage.

scholarly journals Multivariate index of soybean seed vigor: a new biometric approach applied to the effects of genotypes and environments

2018 ◽  
Vol 40 (4) ◽  
pp. 396-406
Author(s):  
Vinícius Jardel Szareski ◽  
Ivan Ricardo Carvalho ◽  
Gustavo Henrique Demari ◽  
Tiago Corazza da Rosa ◽  
Velci Queiróz de Souza ◽  
...  
Keyword(s):  
Seed Production ◽  
Block Design ◽  
Soybean Seed ◽  
Seed Vigor ◽  
Soybean Seeds ◽  
Differential Effects ◽  
Randomized Block Design ◽  
Vigor Index ◽  
Production Environments ◽  
Soybean Genotypes

Abstract : The objective of this work was to apply a multivariate index of vigor in soybean seeds to identify the differential effects of the genotypes x environments interaction on the quality of the seeds produced in the 2016/2017 harvest season. The experiment was conducted in a randomized block design, organized in the factorial scheme, with five seed production environments x 20 soybean genotypes, arranged in four replications. The seed production environments of Tenente Portela - RS and Santa Rosa - RS soybeans are characterized as the environments that express the highest magnitude for seed vigor index, however biometrically Sarandi - RS has been defined as the optimal environment for the production of high vigor seeds according to the tested genotypes. High seed vigor index were expressed for the genotypes TMG 7161 RR, AMS Tibagi RR, BMX Magna RR, Fepagro 37 RR and NA 5909 RG. The differential effects of the genotypes x production environments interaction of soybean seeds influenced by more than 68% the vigor index of the seeds produced.

scholarly journals Vigor-S: System for Automated Analysis of Soybean Seed Vigor

2020 ◽  
Vol 42 ◽  
Author(s):  
Mayara Rodrigues ◽  
Francisco G. Gomes-Junior ◽  
Julio Marcos-Filho
Keyword(s):  
Imaging System ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Field Tests ◽  
Automated Analysis ◽  
Seed Vigor ◽  
Soybean Seeds ◽  
Mean Values ◽  
Randomized Experimental Design

Abstract: Computerized systems for image analysis are alternatives to decrease the subjectivity and speed of assessment of seed physiological potential. The aim of this study was to determine the efficiency of the Seed Vigor Automated Analysis System (Vigor-S) to identify differences in vigor among soybean seed lots compared to results of the Seed Vigor Imaging System - SVIS® and tests recommended for evaluation of soybean seed vigor. Two cultivars were used, BMX Potência RR and 7166 RSF IPRO, each one represented by ten seed lots with similar germination and vigor differences. The seeds were evaluated regarding germination, vigor (tetrazolium, accelerated aging), seedling emergence in the field and SVIS® and Vigor-S analysis, in three experimental periods. Analysis of variance was used on the data in a completely randomized experimental design (laboratory tests) and in randomized blocks (field tests), and the mean values were compared by the Tukey test (p ≤ 0.05). Separation of the seed lots by Vigor-S was consistent with the results obtained in tests recognized as effective in evaluating the physiological potential of soybean seeds, and there was no interference of the different cultivars on the response pattern for vigor. Analysis through Vigor-S proved to be effective in determination of physiological potential and for composition of quality control programs established by soybean seeds production companies.

scholarly journals Alternative procedure for the cold test for soybean seeds

2010 ◽  
Vol 67 (5) ◽  
pp. 540-545 ◽  
Author(s):  
Bruno Guilherme Torres Licursi Vieira ◽  
Roberval Daiton Vieira ◽  
Francisco Carlos Krzyzanowski ◽  
José de Barros França Neto
Keyword(s):  
Glycine Max ◽  
Seed Quality ◽  
Soybean Seed ◽  
Cold Test ◽  
Seed Vigor ◽  
Alternative Procedure ◽  
Test Procedures ◽  
Paper Towel ◽  
Traditional Procedure ◽  
Seed Crops

The growing demand for high quality soybean [Glycine max (L.) Merrill] seeds requires a precise seed quality control system from the seed industry. One way to accomplish this is by improving vigor testing. Cold test has been traditionally employed for corn seeds. However, it has also been used for other seed crops such as cotton (Gossypium spp.), soybean (Glycine Max), dry bean (Phaseolus vulgaris) and pea (Pisum sativum). This study was carried out with the objective of adjusting an alternative procedure for the cold test to determine soybean seed vigor. Six commercial soybean seed lots of the cultivar BRS 133 were used. The physiological potential of the seed lots was evaluated by germination on paper towel and sand box, seedling field emergence, tetrazolium, accelerated aging and electrical conductivity tests. Seed moisture content was also determined. The temperature used for the cold test procedures was 10ºC during five days. Four cold test procedures were evaluated: i) plastic boxes with soil; ii) rolled paper towel with soil; iii) rolled paper towel without soil, and iv) an alternative procedure, using rolled paper towel without soil under cold water. A completely randomized experimental design with eight replications was used and the means were compared by the Tukey test (p = 0.05). To verify the dependence between the alternative test and others single linear correlation was used. All cold test procedures had similar coefficients of variation (CV), highlighting that rolled paper towel with soil and the alternative procedure had the best performance, with an average of 94% and 93% normal seedlings and CV of 3.2% and 3.6%, respectively. The alternative procedure has satisfactory results for estimating soybean seed vigor, yielding consistent results compared to the traditional procedure.

scholarly journals Physiological potential of soybean seeds after maturation and submitted to artificial drying

2017 ◽  
Vol 39 (4) ◽  
pp. 374-384
Author(s):  
Cesar Pedro Hartmann Filho ◽  
André Luís Duarte Goneli ◽  
Tathiana Elisa Masetto ◽  
Elton Aparecido Siqueira Martins ◽  
Guilherme Cardoso Oba
Keyword(s):  
Moisture Content ◽  
Dry Matter ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Maturation Stage ◽  
Soybean Seeds ◽  
Germination Test ◽  
Moisture Contents ◽  
Three Samples ◽  
Maturation Stages

Abstract: This study evaluated the physiological potential of soybean seeds harvested during two seasons, on different maturation stages and subjected to different drying temperatures. The seeds were harvested at the maturations stages R7, R7 + 2, R7 + 3, R7 + 5, R7 + 6, R7 + 7, R7 + 10 and R7 + 12 days (55, 50, 45, 40, 35, 30, 25, and 20% of moisture content). For each maturation stage, seeds were divided into three samples: one sample was used to directly evaluate the physiological potential, and the others were dried at 40 °C and 50 °C, until reaching the moisture content of 11.5%. The physiological potential was evaluated through germination test, first germination count of germination, accelerated aging, modified cold, electrical conductivity and seedling emergence. The maximum physiological potential of seeds is achieved at the moisture content of 55%, the point that the dry matter is maximum. The seeds became tolerant to artificial drying approximately at the stage R7 + 7 days (30% of moisture content). Germination and vigor of the soybean seeds reduce as the drying temperature is increased from 40 °C to 50 °C, and this effect is enhanced when the seeds show moisture contents above 30%.

Sign in / Sign up

Export Citation Format

Share Document