scholarly journals Occurrence of anti Sarcocystis spp., anti Toxoplasma gondii, and anti Neospora spp. antibodies in equines from western Santa Catarina, Brazil

2021 ◽  
Vol 42 (3Supl1) ◽  
pp. 1663-1672
Author(s):  
Alana Birck Ribeiro ◽  
◽  
Maysa Bigolin Chitolina ◽  
Andressa Carminatti ◽  
Fagner D'ambroso Fernandes ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Immunofluorescence ◽  
High Impact ◽  
Serum Samples ◽  
Santa Catarina ◽  
Equine Protozoal Myeloencephalitis ◽  
High Occurrence ◽  
Secondary Activity ◽  
Sarcocystis Spp ◽  
Reproductive Losses

Equine raising is generally articulated as a secondary activity on farms, where animals are used for leisure or work. Thus, it is important to consider the health of these animals, seeking to understand which are the main agents that circulate in a given farm or region. In this sense, this study aimed to evaluate the presence of IgG anti-Sarcocystis spp., anti-Toxoplasma gondii, and anti-Neospora spp. antibodies in equines in western Santa Catarina, Brazil. A total of 100 serum samples from horses from 10 farms, located in the municipalities of Faxinal dos Guedes, Xaxim, and Xanxerê were analyzed from June to December 2019. The samples were evaluated by indirect immunofluorescence reaction (IIFR) to detect antibodies of IgG class against the three agents. The results showed that the equines in the region are seropositive against the three studied agents, 11% of which for Sarcocystis spp. 21% for T. gondii, and 20% for Neospora spp. The analysis of the presence of these protozoa in different environments showed that both stabled and extensively raised horses presented signs of contact with the studied agents. Animals that live only in stables had a higher occurrence of IgG anti-Sarcocystis (7/32; 21.88%) and anti-T. gondii (11/32; 34.38%) antibodies. The evaluating of animals raised on pasture, semi-extensively and extensively, showed a higher percentage of seropositivity for N. caninum (9/34; 26.47%). The high occurrence of animals seropositive to the studied protozoa reinforces the importance of controlling and preventing these agents in the region, as they have the potential to cause a high impact on equid farming, mainly involving reproductive losses and equine protozoal myeloencephalitis (EPM).

scholarly journals Antibodies against Neospora caninum, Sarcocystis spp. and Toxoplasma gondii detected in buffaloes from Rio Grande do Sul, Brazil

2016 ◽  
Vol 36 (10) ◽  
pp. 947-950 ◽  
Author(s):  
Luiza P. Portella ◽  
◽  
Gustavo C. Cadore ◽  
Marcelo de Lima ◽  
Luís A. Sangioni ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Rio Grande ◽  
Antibody Test ◽  
Public Health Issue ◽  
Rio Grande Do Sul ◽  
Serum Samples ◽  
Caninum Infection ◽  
Sarcocystis Spp

ABSTRACT: The presence of antibodies against Neospora caninum, Sarcocystis spp. and Toxoplasma gondii was evaluated in buffaloes (Bubalus bubalis) from Rio Grande do Sul state (RS), southern Brazil. Serum samples (n=220) were analyzed for antibodies by indirect fluorescent antibody test (IFAT). Antibody presence was considered when the titers were equal or higher than 100 for these protozoa. A total of 60.5% (133/220) buffalo serum samples were positive for at least one of the protozoa evaluated in this study. Antibodies for N. caninum, Sarcocystis spp. and T. gondii were found in 36.4% (80/220), 25.5% (56/220) and 16.8% (37/220) of the buffaloes respectively, indicating a higher frequency of N. caninum infection (p=0.0133). The IFAT is a suitable method to diagnose N. caninum, Sarcocystis spp. and T. gondii infection in buffaloes for detecting IgG antibodies. This study demonstrates the presence of these three protozoa in buffalo herds in RS, Brazil, which may be source of infection to other animals. The high frequency of animals positive for N. caninum is important and could be related to reproductive problems. Additionally, the presence of Sarcocystis spp. and T. gondii in buffaloes can be a possible public health issue.

Evaluation of frequency of antibodies against Toxoplasma gondii,Neospora caninum and Sarcocystis spp. and transmission routes in sheep from Humid Pampa, Argentina

2018 ◽  
Vol 63 (2) ◽  
pp. 416-421 ◽  
Author(s):  
Yanina P. Hecker ◽  
Fernando Mogaburu Masson ◽  
Joaquín I. Armendano ◽  
Juan Cora ◽  
Carlos Flores Olivares ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Transmission Route ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Adult Sheep ◽  
Sarcocystis Spp ◽  
Serological Status ◽  
The Impact

AbstractThe aim of this study was to describe the frequency of ovine specific antibodies toToxoplasma gondii,Neospora caninumandSarcocystisspp. and to estimate different transmission routes of these infections. One hundred and thirty Texel sheep and their 117 Texel lambs were included in the study. Serum samples were tested for antibodies toT.gondii,N.caninumandSarcocystisspp. using IFAT.Toxoplasma gondiiseroprevalence was 10.00% in sheep (IC95%: 4.80–15.20%), being higher in adult sheep (≥12 year) than in younger sheep (OR 1.30; 95% CI, 1.10–1.50).N.caninumandSarcocystisspp. seroprevalences were 1.54% (IC95%: 0.00–5.70) and 72.09% (IC95%: 67.70–82.70), respectively, with no association between age and seropositivity in sheep (P>0.05).T.gondiiseroprevalence in lambs was 4.27% (IC95%: 0.61–7.94). No association betweenT.gondiiserological status in sheep and their lambs was detected (P= 0.07). TwoT.gondiiandSarcocystisspp. seropositive lambs were euthanized andT.gondiiandSarcocystisspp. DNA was detected by PCR in their tissues. In conclusion, the increase ofT.gondiiseropositivity in relationship with sheep age and the lack of association between sheep-lamb serological status, suggest that horizontal infection is the main transmission route in this flock as reported before. Due to the low number ofN.caninum-seropositive ewes no assumptions can be done about the impact of this parasite in this flock. According with previous reports, the main transmission route forSarcocystisspp. in this species in the present study was horizontal.

scholarly journals Antibodies against Apicomplexa protozoa and absence sarcocysts in heart tissues from horses in southern Brazil

2017 ◽  
Vol 26 (1) ◽  
pp. 100-103 ◽  
Author(s):  
Luiza Pires Portella ◽  
Gustavo Cauduro Cadore ◽  
Luis Antonio Sangioni ◽  
Luiz Fernando Vilani Pellegrini ◽  
Rafael Fighera ◽  
...  
Keyword(s):  
Life Cycle ◽  
Toxoplasma Gondii ◽  
Myocardial Tissue ◽  
Southern Brazil ◽  
Serum Samples ◽  
Positive Serology ◽  
Tissue Samples ◽  
Sarcocystis Spp ◽  
Direct Microscopic Examination ◽  
Paired Serum

Abstract Sarcocystis spp., Neospora spp., and Toxoplasma gondii are Apicomplexa protozoa that can infect horses. This study aimed to investigate the occurrence of antibodies against Sarcocystis spp., Neospora spp., and T. gondii in horses slaughtered in southern Brazil. The presence of histological lesions, tissue cysts, and Sarcocystis spp. DNA in the hearts of these horses was also investigated. A total of 197 paired serum and heart samples were evaluated by serology and direct microscopic examination; 50 of these samples were subjected to histopathological and PCR analyses. Antibodies against at least one of the protozoa were detected in 146 (74.1%) of the serum samples. The frequencies of positive serology were: 36% (71/197) against Sarcocystis spp., 39.1% (77/197) against Neospora spp., and 47.2% (93/197) against T. gondii. No cysts, Sarcocystis spp. DNA, or histopathological lesions were observed in myocardial tissue samples. The frequencies of antibody seropositivity against Sarcocystis spp., Neospora spp., and T. gondii showed that horses are frequently infected by these parasites in southern Brazil. The absence of sarcocysts in horse tissues is compatible with their role as aberrant/accidental hosts in the life cycle of Sarcocystis spp..

scholarly journals Serological prevalence of Toxoplasma gondii infection in cats (Belém, Pará, Brazil)

2020 ◽  
Vol 29 (2) ◽  
Author(s):  
Katarine de Souza Rocha ◽  
Michele de Souza Lima ◽  
Thamillys Rayssa Marques Monteiro ◽  
Betsy Emely Tavares Honorio ◽  
Ana Paula Vilhena Beckman Pinho ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Immunofluorescence ◽  
Environmental Variables ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Domestic Cats ◽  
Serum Samples ◽  
Toxoplasma Gondii Infection

Abstract We evaluated the prevalence of anti-Toxoplasma gondii antibodies in the serum samples collected from domestic cats in Belém, Pará, Brazil. We also correlated the presence of T. gondii antibodies with environmental variables and cat-owner habits. Four-hundred and forty-seven serum samples from domestic cats were analyzed. The sera were tested using an indirect immunofluorescence assay. Among the animals analyzed, 21.92% (98/447) were seropositive. A statistically significant association was found in relation to age and serology among the animals over 1 year old (p<0.01): in the group up to 1 year old, 12.82% (20/156) of the animals were positive, and in the group over 1 year old, 26.80% (78/291) were positive. Our results show that the cats in Belém, Pará region have anti-T. gondii antibodies, and their owners are not aware of toxoplasmosis or how to prevent its transmission.

scholarly journals Seroprevalence of Toxoplasma gondii and associated risk factors in domestic pigs raised from Cuba

2021 ◽  
Author(s):  
Julio César Castillo-Cuenca ◽  
Álvaro Martínez-Moreno ◽  
José Manuel Diaz-Cao ◽  
Angel Entrena-García ◽  
Jorge Fraga ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Meat Products ◽  
Control Measures ◽  
Health Concern ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Cross Sectional ◽  
Weaning Pigs ◽  
Associated Risk Factors

AbstractA cross-sectional study was carried out to determine the seroprevalence of Toxoplasma gondii and associated risk factors in pigs in the largest pork-producing region in Cuba. Serum samples from 420 pigs, including 210 sows and 210 post-weaning pigs, were tested for antibodies against T. gondii using a commercial indirect enzyme-linked immunosorbent assay. Anti-T. gondii antibodies were detected in 56 animals (13.3%, 95% CI: 10.1–16.6). A generalized estimating equations model revealed that the risk factors associated with higher seropositivity in pigs were altitude (higher in farm’s location < 250 m above sea level (masl) versus ≥ 250 masl) and age (higher in sows compared to post-weaning pigs). The results indicated that this protozoan parasite is widely distributed on pig farms in the study area, which is a public health concern since the consumption of raw or undercooked pork meat products containing tissue cysts is considered one of the main routes of T. gondii transmission worldwide. Control measures should be implemented to reduce the risk of exposure to T. gondii in pigs in Cuba.

scholarly journals A case of human monocytic ehrlichiosis in Serbia

2014 ◽  
Vol 142 (1-2) ◽  
pp. 79-82 ◽  
Author(s):  
Bogdan Arsic ◽  
Ana Gligic ◽  
Elizabeta Ristanovic ◽  
Branislav Lako ◽  
Aleksandar Potkonjak ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Clinical Picture ◽  
Peripheral Blood ◽  
Immunofluorescence Assay ◽  
High Fever ◽  
Tick Bite ◽  
Indirect Immunofluorescence Assay ◽  
Ehrlichia Chaffeensis ◽  
Serum Samples ◽  
History Of

Introduction. Ehrlichiosis is a bacterial zoonosis transmitted by hematophagous arthropods - ticks. In humans, it occurs as monocytic, granulocytic, and ewingii ehrlichiosis. Pathological process is based on parasitic presence of Ehrlichia organisms within peripheral blood cells - monocytes and granulocytes. Case Outline. Fifty-two year old patient was admitted to hospital due to high fever of over 40?C that lasted two days, accompanied with chills, muscle aches, malaise, loss of appetite, headache, confusion, breathing difficulties, and mild dry cough. The history suggested tick bite that occurred seven days before the onset of disease. Doxycycline was introduced and administered for 14 days, causing the disease to subside. Indirect immunofluorescence assay was used to analyze three serum samples obtained from this patient for Ehrlichia chaffeensis antibodies, and peripheral blood smear was evaluated for the presence of Ehrlichia and Ehrlichia aggregation into morulae. Conclusion. Ehrlichiosis should be considered in each case where there is a history of tick bite together with the clinical picture (high fever, chills, muscle aches, headache, generalized weakness and malaise, and possible maculopapular rash). The presence of Ehrlichia chaffeensis antibodies was confirmed in a patient with the history of tick bite, appropriate clinical picture and indirect immunofluorescence assay. This confirmed the presence of human monocytotropic ehrlichiosis, a disease that is uncommonly identified in our country.

scholarly journals Potential linkage between Toxoplasma gondii Infection and physical education scores of College Students

2020 ◽  
Author(s):  
Zhijin Sheng ◽  
Yu Jin ◽  
Yinan Du ◽  
Xinlei Yan ◽  
Yong Yao
Keyword(s):  
College Students ◽  
Physical Education ◽  
Toxoplasma Gondii ◽  
Protozoan Parasite ◽  
Anhui Province ◽  
Behavioral Changes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
School Students ◽  
Toxoplasma Gondii Infection

ABSTRACTObjectiveToxoplasma gondii is a worldwide protozoan parasite that could infect virtually all warm-blooded animals, including humans. Our study aimed to investigate the prevalence of T. gondii infection in college students at Anhui province, China. Moreover, growing studies demonstrated the association between T. gondii infection and host behavioral changes. We also studied the linkage between T. gondii and scores of college students.Methods2704 serum samples of medical school students attending physical education lessons were collected from September 2017 to September 2019 and evaluated for T. gondii IgG antibodies using an enzyme-linked immunosorbent assay (ELISA). We also analysed PE scores of T. gondii infected students and T. gondii uninfected students.ResultsThe overall seroprevalence of T. gondii was 11.5%. The main risk factors related to T. gondii infections were cat in the household and gardening or agriculture activity. Furthermore, in basketball group and football group, scores of T. gondii seropositive students were significantly higher than that of seronegative students, while in other sports there is no difference between scores of T. gondii infected students and T. gondii uninfected students.ConclusionThis is the first report of T. gondii seroprevalence in college students in Anhui province, China.

scholarly journals Presence of antibodies against Toxoplasma gondii, Neospora caninum and Leishmania infantum in dogs from Piauí

2011 ◽  
Vol 20 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Marcos Gomes Lopes ◽  
Ivete Lopes Mendonça ◽  
Kleber Portela Fortes ◽  
Marcos Amaku ◽  
Hilda de Fátima Jesus Pena ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Leishmania Infantum ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Northeastern Brazil ◽  
Leishmania Infection ◽  
Serum Samples ◽  
Chi Square ◽  
Leishmania Spp ◽  
And Gender

This study aimed to evaluate the presence of antibodies against Neospora caninum, Toxoplasma gondii and Leishmania infantum in dogs attended at the Veterinary Hospital of the Federal University of Piauí, Northeastern Brazil, where there are no reports of the occurrence of N. caninum and T. gondii in dogs. Serum samples from 530 dogs of genders, different ages and breeds from the municipality of Teresina and nearby towns were analyzed using three indirect fluorescent antibody tests, each one targeting one of the three agents. The associations between the parasites and gender, breed and age of the dogs were assessed by the chi-square test (p > 0.05). The occurrence of antibodies to N. caninum, T. gondii and L. infantum was 3.2, 18.0 and 78.1%, respectively. Toxoplasma gondii was more frequently found in older dogs (p < 0.05) whereas L. infantum was more common in animals aged between 1 to 3 years (p < 0.05). In order to evaluate potential associations between the presence of anti-N. caninum and anti-T. gondii antibodies and Leishmania infection, 240 dogs were selected (120 positive and 120 negative for Leishmania spp.), based on serological and parasitological diagnoses. No association was found between Leishmania spp. and the coccidian parasites (p > 0.05). The results confirm the exposure of dogs to these parasites in the State of Piauí.

scholarly journals Seroprevalence of Toxoplasma gondii and Encephalitozoon cuniculi among domestic rabbits in central China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 9 ◽  
Author(s):  
Shuai Wang ◽  
Zhijun Yao ◽  
Lingjuan Li ◽  
Yaoqian Pan ◽  
Pengju Li ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Central China ◽  
Enzyme Linked Immunosorbent Assay ◽  
Meat Production ◽  
Health Issues ◽  
Encephalitozoon Cuniculi ◽  
Serum Samples ◽  
Modified Agglutination Test ◽  
Domestic Rabbits

Rabbits (Oryctolagus cuniculus) are frequently reared for meat production in China. The aim of this study was to assess the seroprevalence of Toxoplasma gondii and Encephalitozoon cuniculi, and risk factors of infection in domestic rabbits raised in Henan province, central China. 1,213 serum samples of domestic rabbits were collected and tested for anti-T. gondii and anti-E. cuniculi antibodies using a modified agglutination test (MAT) and an enzyme-linked immunosorbent assay (ELISA), respectively. The serum positive rates of T. gondii and E. cuniculi were 128/1,213 (10.55%) and 235/1,213 (19.37%), respectively. Co-infection of T. gondii and E. cuniculi was demonstrated in 84 specimens; 44 rabbits were seropositive for T. gondii alone, while 151 rabbits were seropositive for E. cuniculi alone. The main risk factors simultaneously associated with T. gondii and E. cuniculi infection were the age of the rabbit, the type of food, and the rabbit rearing system. Serum positive rates of T. gondii and E. cuniculi among domestic rabbits were high, indicating the possibility of public health issues.

Abstract 2099: Pretransplant Toxoplasma Gondii Seropositivity Amongst Heart Transplant Recipients Is Associated With An Increased Risk Of All-cause And Cardiac Mortality

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Satish Arora ◽  
Pål Jenum ◽  
Pål Aukrust ◽  
Halvor Rollag ◽  
Arne Andreassen ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Heart Transplant ◽  
Acute Cellular Rejection ◽  
Serum Samples ◽  
Regulatory Changes ◽  
Risk Of Mortality ◽  
Increased Risk ◽  
Cellular Rejection

Chronic Toxoplasma gondii (T. gondii ) infection is known to trigger potentially adverse immuno-regulatory changes, but the long-term implication for heart transplant (HTx) recipients has not been assessed previously. Hence, we evaluated the risk of mortality, development of cardiac allograft vasculopathy (CAV) and acute cellular rejection amongst T. gondii seropositive HTx recipients and the four donor/recipient seropairing groups. Methods: Frozen pre-HTx serum samples of 288 recipients and 246 donors were evaluated for T. gondii serostatus using Platelia IgG immunoassay method. All patients had also undergone prospective serostatus evaluation using alternative assays and results determined by the two methods were compared. Follow-up data regarding mortality, CAV development and acute cellular rejection was available for all patients. Results: Overall, 211 (73%) recipients were seronegative and 77 (27%) were seropositive. In total, 82 recipients died, 76 developed CAV and 82 had significant cellular rejection. Recipient seropositivity was associated with a significantly higher risk of all-cause mortality (hazard ratio [HR], 1.9; 95% CI, 1.1–3.4; p= 0.02) and CAV mortality (HR=4.4; 95% CI, 1.3–15.6, p=0.02), but was not associated with earlier CAV development or higher rejection score. Donor/recipient seropairing status was not a risk factor for any endpoint. Conclusions: T. gondii seropositivity amongst HTx recipients is associated with a significantly increased risk of long-term total, and in particular CAV-related, mortality. This may be mediated via immunoregulatory changes triggered by chronic T. gondii infection and needs to be explored further.

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