scholarly journals Prevalence, virulence genes, and antimicrobial resistance profile of Listeria monocytogenes isolated from retail poultry shops in Beni-Suef city, Egypt

2020 ◽  
Vol 7 (4) ◽  
pp. 710
Author(s):  
Sahar Aziz ◽  
Manar Mohamed
Keyword(s):  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile

Antimicrobial Resistance Profile of Salmonella and Listeria monocytogenes Isolated from Products Marketed on the Border of Brazil with Argentina and Uruguay

2020 ◽  
Vol 83 (11) ◽  
pp. 1941-1946
Author(s):  
JULIANO GONÇALVES PEREIRA ◽  
VANESSA MENDONÇA SOARES ◽  
LEONARDO ERENO TADIELO ◽  
TASSIANA RAMIRES ◽  
WLADIMIR PADILHA da SILVA
Keyword(s):  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Penicillin G ◽  
Diffusion Method ◽  
Salmonella Spp ◽  
Disk Diffusion Method ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile ◽  
Antimicrobial Resistance Genes

ABSTRACT We aimed to perform serotyping and the antimicrobial resistance profile of Salmonella spp. and Listeria monocytogenes strains isolated from raw meats imported illegally into Brazil along the borders of Argentina and Uruguay. Distinct isolates of Salmonella spp. (n = 6) and L. monocytogenes (n = 25) obtained from 270 of these food products of earlier work were serotyped and tested for antimicrobial resistance by agar disk diffusion method. For strains that were considered phenotypically resistant, antimicrobial resistance genes were investigated: strA, strB, floR, tetA, tetB, blaZ, blaTEM, ermB, ermC, and ereB to Salmonella sp. and blaZ and mecA to L. monocytogenes. All Salmonella isolates were identified as Salmonella Infantis; they were multidrug resistant and harbored the genes blaTEM (n = 6), strA (n = 1), strB (n = 1), floR (n = 1), ermB (n = 1), tetA (n = 3), and tetB (n = 3). L. monocytogenes isolates belonged to serovars 1/2a (n = 1), 1/2b (n = 14), 1/2c (n = 2), and 4b (n = 8), showed resistance only to penicillin G (n = 12), and did not show the blaZ and mecA genes. The results demonstrated that illegal foods that are commercialized in the Brazilian international border with Argentina and Uruguay may harbor foodborne pathogens, and some of them have multidrug resistance characteristics, such as Salmonella, emphasizing the need for greater control of international food transit in Brazil, especially in the region evaluated. HIGHLIGHTS

scholarly journals Antimicrobial resistance and genetic relationships of enterococci from siblings and non-siblings Heliconius erato phyllis caterpillars

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8647 ◽  
Author(s):  
Rosana Huff ◽  
Rebeca Inhoque Pereira ◽  
Caroline Pissetti ◽  
Aldo Mellender de Araújo ◽  
Pedro Alves d’Azevedo ◽  
...  
Keyword(s):  
Microbial Community ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Genetic Relationships ◽  
Selective Medium ◽  
Forest Fragment ◽  
Fecal Samples ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile ◽  
Enterococcus Casseliflavus

Background Studies evaluating bacteria in insects can provide information about host–microorganism–environment interactions. The gut microbial community has a profound effect on different physiological functions of insects. Enterococcus spp. are part of the gut community in humans and other animals, as well as in insects. The presence and antimicrobial resistance profile of enterococci are well studied in different animals; however, data for Heliconius erato phyllis (Lepidoptera: Nymphalidae) do not yet exist. Therefore, the aims of this study were to evaluate the distribution of enterococcal species, their antimicrobial resistance profile and virulence genes, and the genetic relationships between enterococci isolated from fecal samples from sibling and non-sibling H. erato phyllis caterpillars collected from different sites in South Brazil. Methods Three H. erato phyllis females were captured (two from a forest fragment and one from an urban area), and kept individually in open-air insectaries. Eggs were collected and caterpillars (siblings and non-siblings) were fed daily with Passiflora suberosa leaves. Fecal samples (n = 12) were collected from fifth-instar caterpillars, inoculated in selective medium, and 15 bacterial colonies were randomly selected from each sample. Enterococci were identified by PCR and MALDI-TOF, analyzed by disk diffusion antimicrobial susceptibility tests, and screened for resistance and virulence genes by PCR. The genetic relationships between the strains were determined using pulsed-field gel electrophoresis (PFGE). Results A total of 178 enterococci strains were identified: E. casseliflavus (74.15%; n = 132), E. mundtii (21.34%; n = 38), E. faecalis (1.12%; n = 2) and Enterococcus sp. (3.37%; n = 6). High rates of resistance to rifampicin (56%) and erythromycin (31%) were observed; 120 (67.41%) of the isolates showed resistance to at least one antibiotic and six (3.37%) were multidrug-resistant.None of the erythromycin-resistant strains was positive for the erm(B) and msrC genes. The virulence genes esp, ace, and gelE were observed in 35%, 7%, and 1% of the strains, respectively. PFGE separated the enterococci into 22 patterns, four being composed of strains from sibling caterpillars. Conclusion Enterococcus casseliflavus was the dominant species in fecal samples of fifth-instar caterpillars. Resistant enterococci strains may be related to environmental pollution or the resistome. The PFGE analysis showed genetic relationships between some strains, suggesting that the enterococci isolated from fecal samples of the sibling caterpillars might have come from common sources, e.g., via diet (herbivory) and/or vertical transmission (through the egg surface). Further studies will be conducted to better understand the role of Enterococcus in the microbial community of the gastrointestinal tract of these insects, and the mechanisms involved in acquisition and maintenance of enterococci.

scholarly journals Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products

2010 ◽  
Vol 43 (4) ◽  
pp. 382-385 ◽  
Author(s):  
Fernanda De Nes ◽  
Gustavo Pelicioli Riboldi ◽  
Ana Paula Guedes Frazzon ◽  
Pedro Alves d'Azevedo ◽  
Jeverson Frazzon
Keyword(s):  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Dairy Products ◽  
Molecular Techniques ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile ◽  
Random Amplification ◽  
Contaminated Food

INTRODUCTION: Listeria monocytogenes is a ubiquitous microorganism in nature and is responsible for listeriosis, an infectious disease caused by consumption of contaminated food. METHODS: Molecular characterization was performed on 19 strains of Listeria monocytogenes (serovars 1/2a, 1/2b, 4b and 4c), isolated from dairy products in Rio Grande do Sul, Brazil. The molecular techniques applied were random amplification of polymorphic DNA and restriction enzyme analysis. In addition to the molecular analysis, the antimicrobial resistance profile was determined. RESULTS: The strains studied showed a low degree of diversity. In relation to the antimicrobial resistance profile of those microorganisms from the samples analyzed, all of them were susceptible to the antimicrobials tested. CONCLUSIONS: The molecular techniques that were used presented good discriminatory power for the strains studied. Furthermore, all of the samples that were analyzed were susceptible to the antimicrobials tested.

scholarly journals Prevalence and Antimicrobial Resistance Profile of Listeria monocytogenes from Retailed Fresh Cat Fish and Frozen Fish in Abuja, Nigeria.

2021 ◽  
Vol 3 (1) ◽  
pp. 53-62
Author(s):  
J. A Alu
Keyword(s):  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Bacterial Resistance ◽  
Fish Population ◽  
Latex Agglutination ◽  
Sensitivity Testing ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile ◽  
Significant Difference ◽  
Frozen Fish

This study investigated the prevalence and antibiotic resistance profile of Listeria monocytogenes from fresh catfish (Clarias species) and frozen raw fish (Mackerel and Herring). A total of 180 samples which comprised 60 units of fresh catfish, mackerel, and herring frozen fish each distributed uniformly across three locations in Abuja, were randomly collected within a period of three months. The samples were analyzed using standard bacteriological methods at the Animal science/bacteriology laboratory, Faculty of Agriculture, University of Abuja. Presumptive isolates were further serotyped using latex agglutination and subjected to antimicrobial sensitivity testing at the Advance Biotechnology laboratory (SHESTCO) Sheda, Abuja. Overall prevalence of L. monocytogenes in this study was 10.6% (n=19/180) distributed across Gwagwalada (20%), Bwari (11.7%) and none in Kwali Area council. The occurrence according to fish types showed 18.3% in mackerel, 10.0% in herring fish while 3.33% was from catfish. There was no statistically significant difference (p>0.05) between the prevalence of Listeria monocytogenes in the fish type studied. Antimicrobial resistance profile indicated high MAR index (≥ 0.4) which revealed a diverse spread of bacterial resistance to antibiotics within the fish population in the study area. This was characterized by 100% resistance of L. monocytogenes isolates to ampicillin and tetracycline while few (5) isolates were susceptible to chloramphenicol (71.5%), streptomycin (61.2%). This finding provides a baseline information on the prevalence and antibiotic profile of L. monocytogenes in catfish and frozen fish in Abuja, Nigeria. Proper hygienic handling of fish during processing and sales is optimum in mitigating the risk of foodborne illness due to L. monocytogenes. Adequate control of antimicrobial agent usage is also recommended to reduce the occurrence and spread of potential multidrug resistance strains.

Antimicrobial resistance profile of Trueperella pyogenes associated with slaughterhouse condemnations of pigs in Spain

2017 ◽  
Author(s):  
Ángela Galán-Relaño ◽  
Belén Barrero-Domínguez ◽  
Almudena Casamayor ◽  
Fernando Cardoso-Toset ◽  
Ana Lucía Solarte ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Resistance Profile ◽  
Trueperella Pyogenes ◽  
Antimicrobial Resistance Profile

scholarly journals Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 288
Author(s):  
Sonia Sciortino ◽  
Pietro Arculeo ◽  
Vincenzina Alio ◽  
Cinzia Cardamone ◽  
Luisa Nicastro ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Tetracycline Resistance ◽  
Multi Drug Resistance ◽  
Genetic Determinants ◽  
Antibiotic Resistant ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile ◽  
Drug Resistance Profile ◽  
Arcobacter Butzleri ◽  
Seawater Samples

Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence and the antimicrobial resistance profile of Arcobacter isolates recovered from different aquatic sources. Besides, we searched for Arcobacter spp. in seaweeds and the corresponding seawater samples. Bacteriological and molecular methods applied to 100 samples led to the isolation of 28 Arcobacter isolates from 27 samples. The highest prevalence was detected in rivers followed by artificial ponds, streams, well waters, and spring waters. Seaweeds contained a higher percentage of Arcobacter than the corresponding seawater samples. The isolates were identified as Arcobacter butzleri (96.4%) and Arcobacter cryaerophilus (3.6%). All the isolates showed a multi-drug resistance profile, being resistant to at least three different classes of antibiotics. Molecular analysis of genetic determinants responsible for tetracycline resistance in nine randomly chosen isolates revealed the presence of tetO and/or tetW. This work confirms the occurrence and the continuous emergence of antibiotic-resistant Arcobacter strains in environmental samples; also, the presence of quinolone-resistant Arcobacter spp. in aquatic sources used for water supply and irrigation represents a potential risk for human health.

scholarly journals Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

2015 ◽  
Vol 35 (9) ◽  
pp. 775-780 ◽  
Author(s):  
Marcos R.A. Ferreira ◽  
Talícia dos S. Silva ◽  
Ariel E. Stella ◽  
Fabricio R. Conceição ◽  
Edésio F. dos Reis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
Shiga Toxin ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile ◽  
Healthy Sheep ◽  
Resistance Patterns ◽  
Group A ◽  
Stx1 Gene

Abstract: In order to detect virulence factors in Shiga toxin-producing Escherichia coli (STEC) isolates and investigate the antimicrobial resistance profile, rectal swabs were collected from healthy sheep of the races Santa Inês and Dorper. Of the 115 E. coli isolates obtained, 78.3% (90/115) were characterized as STEC, of which 52.2% (47/90) carried stx1 gene, 33.3% (30/90) stx2 and 14.5% (13/90) both genes. In search of virulence factors, 47.7% and 32.2% of the isolates carried the genes saa and cnf1. According to the analysis of the antimicrobial resistance profile, 83.3% (75/90) were resistant to at least one of the antibiotics tested. In phylogenetic classification grouped 24.4% (22/90) in group D (pathogenic), 32.2% (29/90) in group B1 (commensal) and 43.3% (39/90) in group A (commensal). The presence of several virulence factors as well as the high number of multiresistant isolates found in this study support the statement that sheep are potential carriers of pathogens threatening public health.

scholarly journals Antimicrobial Resistance Profile of Escherichia coli Isolates from Different Clinical Samples in Abeokuta, Ogun State

2020 ◽  
pp. 12-18
Author(s):  
S. L. Owolabi ◽  
I. A. Azeez
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Multiple Drug Resistance ◽  
Resistance Rate ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Resistance Profile ◽  
E Coli ◽  
Ogun State ◽  
Antimicrobial Resistance Profile

The alarming increase of antibiotic resistance of Escherichia coli has posed a great challenge in the public health sector. Thus, this microorganism is a leading cause of different human infections and it can be found in various environments. The aim of this study is to investigate the antimicrobial susceptibility patterns and the multiple antimicrobial resistance profile of Escherichia coli isolates obtained from some hospitals in Abeokuta, Ogun State, Nigeria. Isolates of E. coli were obtained from different clinical samples and were re-identified morphologically and biochemically. E. coli was isolated from 30% out of a total of 70 clinical samples analyzed for isolation and identification. The isolation rate of E. coli was highest in urine samples 10(47.6%) when compared to other clinical samples. There was significant increase in the resistance rate of E. coli to tetracycline (14.3%), ceftazidime (14.2%), and ampicillin (14.2%).Also, an increased sensitivity rate to augmentin (71.4%), ofloxacin (66.7%), cefuroxime (66.7%), ciprofloxacin (61.9%) and ceftazidime (61.9%) were observed. Furthermore, the overall multiple drug resistance rates obtained was 14(66.7%) and it was established that, multiple antimicrobial resistance of the E. coli isolates was plasmid mediated. E. coli isolates exhibited high resistance rate to multiple antimicrobial agents, however, its sensitivity to augmentin, ofloxacin, cefuroxime, ciprofloxacin and ceftazidime showed that these antimicrobials are still effective against E. coli infections in the study area.

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