scholarly journals Preliminary study on antioxidant properties, phenolic contents, and effects of Aesculus hippocastanum (horse chestnut) seed shell extract on in vitro cyclobutane pyrimidine dimer repair

2017 ◽  
Vol 6 (4) ◽  
pp. 1 ◽  
Author(s):  
Masatomo Makino ◽  
Takuya Katsube ◽  
Yukari Ohta ◽  
Werner Schmidt ◽  
Katsumi Yoshino
Keyword(s):  
Antioxidant Properties ◽  
Pyrimidine Dimer ◽  
Aesculus Hippocastanum ◽  
Horse Chestnut ◽  
Cyclobutane Pyrimidine Dimer ◽  
Phenolic Contents ◽  
Preliminary Study ◽  
Horse Chestnut Seed

scholarly journals In vitro permeation studies of phenolics from horse chestnut seed gels prepared with different polyacrylic acid polymer derivatives

2015 ◽  
Vol 65 (2) ◽  
pp. 181-189 ◽  
Author(s):  
Eglė Zelbienė ◽  
Gailute Draksiene ◽  
Arunas Savickas ◽  
Dalia Kopustinskiene ◽  
Ruta Masteikova ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Polyacrylic Acid ◽  
Mentha Piperita ◽  
Horse Chestnut ◽  
Peppermint Oil ◽  
In Vitro Permeation ◽  
Carbopol 940 ◽  
Permeation Studies ◽  
Horse Chestnut Seed

Abstract The aim of this study was to investigate the effects of polyacrylic acid polymers (Ultrez 10, Ultrez 20, Carbopol 980, and Carbopol 940) on the viscosity and the in vitro permeation of phenolic compounds from the gel prepared from natural horse chestnut seed extract. Experiments were performed in the presence and in the absence of peppermint oil (Mentha piperita). Our results showed that peppermint oil decreased the viscosity of the gels and permeation of phenolic compounds from all gel samples. Results show that the highest content of phenolic compounds (1.758 μg cm-2) permeated in vitro from gel based on Carbopol Ultrez 20 without peppermint oil added (p < 0.05 vs. other tested polymers).

scholarly journals SPECIFICITY OF CYTODIFFERENTIATION IN СALLUSES IN VITRO OF AESCULUS HIPPOCASTANUM FORM RESISTANT TO HORSE-CHESTNUT LEAF MINER

2017 ◽  
Vol 49 (6) ◽  
pp. 495-505
Author(s):  
A.F. Likhanov ◽  
◽  
O.V. Overchenko ◽  
S.M. Kostenko ◽  
O.V. Subin ◽  
...  
Keyword(s):  
Leaf Miner ◽  
Aesculus Hippocastanum ◽  
Horse Chestnut ◽  
Chestnut Leaf

scholarly journals Comparative Studies of Phytochemicals Analysis and in vitro Antioxidant Properties of Various Solvent Extracts of Sphaeranthus indicus Collected from three Different Climatic Zones of Central India

2021 ◽  
Vol 33 (9) ◽  
pp. 2237-2243
Author(s):  
Pooja N. Akhand ◽  
Veena Sharma ◽  
Anupam K. Pathak
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Central India ◽  
Total Phenolic ◽  
Climatic Zones ◽  
Phenolic Contents ◽  
Total Antioxidant ◽  
Sphaeranthus Indicus

In present study, the total phenolic, flavonoid, tannin contents and the antioxidant activity of various solvent extracts of Sphaeranthus indicus collected from three different regions of central India were assessed. Plants extracts were prepared using Soxhlation method, while the total phenols, flavonoids and tannins were measured by the spectrophotometric method. DPPH, metal chelating, nitric oxide, superoxide oxide scavenging activity and FRAP, reducing power, total antioxidant assays were also evaluated. The highest phenolic contents 268.22 GAE equivalent, flavonoids 441.33 QE equivalents and tannin content was 120.32 tannic acid equivalents (mg/g) obtained from ethanolic extracts of sample SIEE-1 compared to other two plants extract. Similarly, the highest flavonoid contents was observed in SIAE-1 aqueous extract and lowest in SIAE-2 and SIAE-3. Extract of SIEE-1 possessed maximum antioxidant potentiality and SIAE-2 shown the least antioxidant activity in all assays. It could be concluded that different agroclimatic conditions have effects on the total phenolics, flavonoids, tannin contents and antioxidant potentiality of S. indicus plant.

scholarly journals Phenolic Extract fromMoringa oleiferaLeaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile Tissues

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Ganiyu Oboh ◽  
Adedayo O. Ademiluyi ◽  
Ayokunle O. Ademosun ◽  
Tosin A. Olasehinde ◽  
Sunday I. Oyeleye ◽  
...  
Keyword(s):  
Erectile Dysfunction ◽  
High Performance ◽  
Antioxidant Properties ◽  
Tissue Homogenate ◽  
Phenolic Contents ◽  
Phenolic Constituents ◽  
Array Detection ◽  
Phenolic Extract ◽  
Dose Dependent

This study was designed to determine the antioxidant properties and inhibitory effects of extract fromMoringa oleiferaleaves on angiotensin-I-converting enzyme (ACE) and arginase activitiesin vitro. The extract was prepared and phenolic (total phenols and flavonoid) contents, radical (nitric oxide (NO), hydroxyl (OH)) scavenging abilities, and Fe2+-chelating ability were assessed. Characterization of the phenolic constituents was done via high performance liquid chromatography-diode array detection (HPLC-DAD) analysis. Furthermore, the effects of the extract on Fe2+-induced MDA production in rats’ penile tissue homogenate as well as its action on ACE and arginase activities were also determined. The extract scavengedNO∗,OH∗, chelated Fe2+, and inhibited MDA production in a dose-dependent pattern with IC50values of 1.36, 0.52, and 0.38 mg/mL and 194.23 µg/mL, respectively. Gallic acid, chlorogenic acid, quercetin, and kaempferol were the most abundant phenolic compounds identified in the leaf extract. The extract also inhibited ACE and arginase activities in a dose-dependent pattern and their IC50values were 303.03 and 159.59 µg/mL, respectively. The phenolic contents, inhibition of ACE, arginase, and Fe2+-induced MDA production, and radical (OH∗,NO∗) scavenging and Fe2+-chelating abilities could be some of the possible mechanisms by whichM. oleiferaleaves could be used in the treatment and/or management of erectile dysfunction.

scholarly journals Identification of Cyclobutane Pyrimidine Dimer-Responsive Genes Using UVB-Irradiated Human Keratinocytes Transfected with In Vitro-Synthesized Photolyase mRNA

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0131141 ◽  
Author(s):  
Gábor Boros ◽  
Edit Miko ◽  
Hiromi Muramatsu ◽  
Drew Weissman ◽  
Eszter Emri ◽  
...  
Keyword(s):  
Human Keratinocytes ◽  
Pyrimidine Dimer ◽  
Cyclobutane Pyrimidine Dimer

scholarly journals Phenolic Compounds of Soybean Seeds from Two European Countries and Their Antioxidant Properties

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2075
Author(s):  
Angelika Król-Grzymała ◽  
Ryszard Amarowicz
Keyword(s):  
Phenolic Compounds ◽  
Defense Mechanism ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Cold Climate ◽  
Total Phenolic ◽  
Soybean Seeds ◽  
Phenolic Contents ◽  
Soybean Cultivars

There is only a small acreage of planted soybeans in northern Europe, as the global production of this crop is mainly dictated by the warmer temperatures needed for bountiful yields. The defense response of soybean plants to a cold climate entails the secretion of specific compounds which help mitigate oxidative stress, i.e., antioxidants, including phenolic compounds. The objective of this study was to examine differences in the concentrations of phenolic compounds, their antioxidant properties, and the concentration of key isoflavones (namely genistein, daidzein, malonyl daidzein, malonyl genistein, and daidzin) in the seeds of six soybean cultivars from two different regions of Europe, namely Poland and France. The total phenolic contents, isoflavone levels, and in vitro antioxidant capacities of soybean seeds from most of the investigated cultivars of northeast Europe were found to be greater than those from southwest Europe. The phenolic compounds of seed extracts are primarily responsible for the free-radical scavenging of soybeans. Factors regulating the production of phenolic compounds in the seeds have not been thoroughly elucidated. Hence, the results presented in this paper can be useful in the selection of soybean cultivars with higher levels of seed phenolics, because of their beneficial impact on human health and on the soybean’s defense mechanism against plant stresses.

scholarly journals Biochemical composition of the horse chestnut seed (Aesculus hippocastanum L.)

2011 ◽  
Vol 63 (2) ◽  
pp. 345-351 ◽  
Author(s):  
Jelena Cukanovic ◽  
Jelena Ninic-Todorovic ◽  
V. Ognjanov ◽  
Emina Mladenovic ◽  
Mirjana Ljubojevic ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Urban Areas ◽  
Biochemical Composition ◽  
Aesculus Hippocastanum ◽  
Horse Chestnut ◽  
High Grade ◽  
Statistical Parameters ◽  
Reproductive Material ◽  
Horse Chestnut Seed ◽  
Novi Sad

This study presents research on the chemical composition of 15 horse chestnut seed genotypes (Aesculus hippocastanum L.) conducted on three different sites. The seeds were picked from selected trees in Backa Palanka, Novi Sad and Sremski Karlovci at the end of September 2010. The statistical parameters point out the variables of the examined features of the seed. On the basis of the moisture, starch, fat and protein contents in the horse chestnut seeds, the genotypes, the reproductive material which would be used for the intensive production of the high grade seedlings for the greening of the different area categories in urban areas, are distinguished.

scholarly journals Bioactivity Potential of Aesculus hippocastanum L. Flower: Phytochemical Profile, Antiradical Capacity and Protective Effects on Human Plasma Components under Oxidative/Nitrative Stress In Vitro

2021 ◽  
Vol 14 (12) ◽  
pp. 1301
Author(s):  
Aleksandra Owczarek ◽  
Joanna Kołodziejczyk-Czepas ◽  
Paulina Marczuk ◽  
Julia Siwek ◽  
Katarzyna Wąsowicz ◽  
...  
Keyword(s):  
Human Plasma ◽  
Thiobarbituric Acid ◽  
Aesculus Hippocastanum ◽  
Horse Chestnut ◽  
Protective Effects ◽  
Total Polyphenol ◽  
Nitrative Stress ◽  
Kaempferol Glycosides ◽  
First Time

Horse chestnut (Aesculus hippocastanum) flower is a traditional medicine applied to alleviate symptoms of chronic venous insufficiency (CVI). However, its flavonoid-based composition has not been sufficiently recognized, and the data supporting its traditional application are lacking. In the work, 43 constituents were detected by UHPLC–PDA–ESI–TQ–MS/MS (flavonoids, phenolic acids, flavanols, and coumarins), including 31 reported in the flower for the first time. The quantitative HPLC–PDA study (developed and validated for quality control purposes) indicated the fractionated extraction as an efficient method for enhancing the total polyphenol content (TPHC) in the extracts (up to 414.06 mg/g) and kaempferol glycosides as their dominant constituents (75.05–82.14% TPHC). The activity studies showed significant scavenging properties of the extracts and their constituents towards reactive oxygen species (especially against highly reactive hydroxyl radical, with capacities up to 7.85 mmol ascorbic acid equivalents/g). Moreover, the analytes relevantly protected human plasma biomolecules from peroxynitrite-induced oxidative/nitrative damage; at 1–50 µg/mL, they hindered the protein nitration and lipid peroxidation, decreasing the levels of 3-nitrotyrosine (by up to 50%) and thiobarbituric acid reactive substances (by up to 70%), respectively. The extracts also averted the depletion of plasma thiols (by up to 67%) and improved the non-enzymatic antioxidant capacity of plasma. The demonstrated mechanisms might be partly responsible for the efficacy of the flower in CVI. Additionally, the anti-aggregatory and anticoagulant properties of the extracts were found only mild or negligible, which suggests that they may be safely applied with drugs impacting the coagulation process.

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