scholarly journals PHÂN LẬP VÀ TUYỂN CHỌN CÁC CHỦNG XẠ KHUẨN CÓ KHẢ NĂNG SINH ENZYME NGOẠI BÀO, ĐỐI KHÁNG VIBRIO SPP.

2021 ◽  
Vol 18 (6) ◽  
pp. 1016
Author(s):  
Tô Đình Phúc ◽  
Nguyễn Thị Thuý Hương ◽  
Phan Thị Thu Đan ◽  
Trương Thị Mỹ Phượng
Keyword(s):  
16S Rrna ◽  
Streptomyces Hygroscopicus ◽  
Vibrio Spp

  Nghiên cứu được tiến hành nhằm phân lập và tuyển chọn các chủng xạ khuẩn từ các ao nuôi tôm thẻ chân trắng ở huyện Thạnh Phú, tỉnh Bến Tre có khả năng sinh enzyme ngoại bào và đối kháng với Vibrio spp. Có tất cả 26 chủng xạ khuẩn được phân lập, trong đó 5 chủng TM1, TM2, TM7, TM21 và TM22 được xác định đều là các chủng đa chức năng. Cả 5 chủng này đều có khả năng sản sinh tốt cả 3 loại enzyme protease, amylase và cellulase. Đặc biệt, 3 chủng TM1, TM2 và TM21 còn có khả năng đối kháng với Vibrio spp.. Phân tích kết quả giải trình tự gen 16S rRNA cho thấy cả 3 chủng TM1, TM2, TM21 đều thuộc loài Streptomyces hygroscopicus. Hai chủng TM7 và TM22 được xác định lần lượt là Streptomyces diastaticus và Streptomyces spiralis. 

scholarly journals Gut Symbiotic Microbial Communities in the IUCN Critically Endangered Pinna nobilis Suffering from Mass Mortalities, Revealed by 16S rRNA Amplicon NGS

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1002
Author(s):  
Athanasios Lattos ◽  
Ioannis A. Giantsis ◽  
Dimitrios Karagiannis ◽  
John A. Theodorou ◽  
Basile Michaelidis
Keyword(s):  
16S Rrna ◽  
Synergistic Effects ◽  
Disease Outbreaks ◽  
Healthy Population ◽  
Emerging Pathogens ◽  
Pinna Nobilis ◽  
Next Generation Sequencing Ngs ◽  
First Time ◽  
Vibrio Spp ◽  
Of Greece

Mass mortality events due to disease outbreaks have recently affected almost every healthy population of fan mussel, Pinna nobilis in Mediterranean Sea. The devastating mortality of the species has turned the interest of the research towards the causes of these events. After the haplosporidan infestation and the infection by Mycobacterium sp., new emerging pathogens have arisen based on the latest research. In the present study, a metagenomic approach of 16S rRNA next generation sequencing (NGS) was applied in order to assess the bacterial diversity within the digestive gland of diseased individuals as well as to carry out geographical correlations among the biodiversity of microbiome in the endangered species Pinna nobilis. The specimens originated from the mortalities occurred in 2019 in the region of Greece. Together with other bacterial genera, the results confirmed the presence of Vibrio spp., assuming synergistic effects in the mortality events of the species. Alongside with the presence of Vibrio spp., numerous bacterial genera were detected as well, including Aliivibrio spp., Photobacterium spp., Pseudoalteromonas spp., Psychrilyobacter spp. and Mycoplasma spp. Bacteria of the genus Mycoplasma were in high abundance particularly in the sample originated from Limnos island representing the first time recorded in Pinna nobilis. In conclusion, apart from exclusively the Haplosporidan and the Mycobacterium parasites, the presence of potentially pathogenic bacterial taxa detected, such as Vibrio spp., Photobactrium spp. and Alivibrio spp. lead us to assume that mortality events in the endangered Fan mussel, Pinna nobilis, may be attributed to synergistic effects of more pathogens.

scholarly journals Colony morphology and molecular identification of Vibrio spp. on green mussels (Perna viridis) in Yogyakarta, Indonesia tourism beach areas

2019 ◽  
Vol 20 (10) ◽  
Author(s):  
FARIDA HIKMAWATI ◽  
Ari Susilowati ◽  
RATNA SETYANINGSIH
Keyword(s):  
16S Rrna ◽  
Molecular Identification ◽  
Pathogenic Bacteria ◽  
Morphological Characteristics ◽  
Vibrio Species ◽  
Colony Morphology ◽  
Perna Viridis ◽  
Rrna Gene ◽  
Coastal Tourism ◽  
Vibrio Spp

Abstract. Hikmawati F, Susilowati A, Setyaningsih R. 2019. Colony morphology and molecular identification of Vibrio spp. on green mussels (Perna viridis) in Yogyakarta, Indonesia tourism beach areas. Biodiversitas 20: 2891-2899. Green mussels (Perna viridis) have filter feeder properties that allow pathogenic bacteria from the water environment to accumulate in relatively high levels. About 20% of foodborne diseases are caused by large quantities of seafood contaminated with bacteria. The purpose of this study is to determine the morphological characteristics, pathogenicity, identity, and the kinship of Vibrio species on green mussels in Yogyakarta coastal tourism areas. Vibrio spp. were grown on selective differential TCBS media. In this media, the suspected Vibrio spp. would produce yellow or green colonies. The ability of hemolysis of Vibrio was blood agar media, the species was molecularly identified using 16S rRNA gene sequence, and the phylogenetic relationship of the Vibrio spp., was analyzed using MEGA X Neighbor-Joining program. Based on morphological analysis, we obtained 23 bacterial isolates suspected to be Vibrio spp. Two Isolates (L1K2 6 and L2K2 13) were positive for α-hemolysis activity and 4 isolates (L1K1 3, L2K1 8, L2K2 16, and L3K2 22) were positive for β-hemolysis activity. The molecular analysis involved 18 Vibrio species, and 4 of them represented the Vibrio genus and 14 species represented 97-99% similarity species in accordance with the 16S rRNA sequence in database, namely: Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio cholerae, Vibrio neocaledonicus, Vibrio mimicus, Vibrio azureus, Vibrio diabolicus, Vibrio tapetis, Vibrio natriegens, and Vibrio owensii. The most dominant number of Vibrio isolates was V. alginolyticus, while the lowest was V. owensii. The highest number of Vibrio species in green mussels was found in Goa Cemara beach while the lowest was in Kwaru beach. Vibrio spp bacteria found in green clams in coastal tourism areas in Yogyakarta have close phylogenetic relationships with other Vibrio in seafood in Indonesian coastal waters.

scholarly journals PHÂN LẬP VÀ KIỂM TRA KHẢ NĂNG KHÁNG VI KHUẨN GÂY BỆNH CỦA Lactococcus garvieae TỪ HỆ TIÊU HÓA TÔM

2019 ◽  
Vol 128 (1E) ◽  
pp. 77-86
Author(s):  
Nguyễn Đức Huy ◽  
Lê Mỹ Tiểu Ngọc ◽  
Đặng Quang Nguyên ◽  
Đỗ Thị Hương Duyên ◽  
Trần Thúy Lan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
16S Rrna ◽  
Vibrio Parahaemolyticus ◽  
Viet Nam ◽  
E Coli ◽  
Lactococcus Garvieae ◽  
Vibrio Spp

Sử dụng chế phẩm sinh học đối kháng tác nhân gây bệnh thay thế kháng sinh là định hướng nghiên cứu có tiềm năng cao trong ứng dụng kiểm soát vi khuẩn gây bệnh. Qua quá trình phân lập và sàng lọc ban đầu, chúng tôi thu được 17 chủng vi khuẩn lactic có khả năng ức chế Vibrio parahaemolyticus từ 23 mẫu hệ tiêu hóa tôm thu thập trên địa bàn tỉnh Thừa Thiên Huế. Kết quả phân tích trình tự nucleotide của đoạn 16S rRNA cho thấy 4 chủng tương đồng cao với Lactococcus garvieae. Chủng phân lập có hoạt tính mạnh nhất được sử dụng để đánh giá hoạt tính ức chế sự sinh trưởng cộng đồng vi khuẩn Vibrio spp., Escherichia coli ATCC 85922 và Staphylococcus aureus ATCC 25023. Đường kính vòng kháng khuẩn cho thấy các chủng phân lập có khả năng ức chế với nhiều loại Vibrio sp. khác nhau với đường kính vòng đối kháng lớn nhất đạt 23 mm. Trong khi đó, khả năng đối kháng của chủng vi khuẩn phân lập này đối với S. aureus ATCC 25023 và E. coli ATCC 85922 đạt đường kính vòng lần lượt là 15 mm và 11 mm. Đây là nghiên cứu đầu tiên ở Việt Nam sử dụng L. garvieae đối kháng nhóm vi sinh vật gây bệnh trên tôm và động vật thủy sản.

scholarly journals First Report of Potential Coral Disease in the Coral Hatchery of Thailand

Diversity ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 18
Author(s):  
Suppakarn Jandang ◽  
Dewi E. Bulan ◽  
Suchana Chavanich ◽  
Voranop Viyakarn ◽  
Kornrawee Aiemsomboon ◽  
...  
Keyword(s):  
16S Rrna ◽  
Coral Disease ◽  
Molecular Techniques ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
16S Rrna Gene Analysis ◽  
Strain Isolation ◽  
Natural Reefs ◽  
Vibrio Spp ◽  
The 16S Rrna Gene

In this study, coral disease was first reported in the coral hatchery in Thailand. Disease were usually found on corals aged two to five years old during the months of November to December of each year. To identify bacterial strains, culture-based methods for strain isolation and molecular techniques of the 16S rRNA gene analysis were used. The resuts showed that the dominant genera of bacteria in diseased corals were Vibrio spp. (comprising 41.01% of the isolates). The occurrence of the disease in the coral hatchery can have a significant effect on the health and survival of juvenile corals before being transplanted to natural reefs for restoration.

scholarly journals Reclassification of Streptomyces hygroscopicus strains as Streptomyces aldersoniae sp. nov., Streptomyces angustmyceticus sp. nov., comb. nov., Streptomyces ascomycinicus sp. nov., Streptomyces decoyicus sp. nov., comb. nov., Streptomyces milbemycinicus sp. nov. and Streptomyces wellingtoniae sp. nov.

2010 ◽  
Vol 60 (4) ◽  
pp. 769-775 ◽  
Author(s):  
Yashawant Kumar ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Streptomyces Hygroscopicus ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data

A polyphasic study was undertaken to determine the taxonomic status of six strains received as Streptomyces hygroscopicus. The strains had chemotaxonomic and morphological properties typical of members of the genus Streptomyces and formed distinct phyletic lines in the Streptomyces 16S rRNA gene tree. These strains were distinguished from one another and from phylogenetically close neighbours using a combination of phenotypic properties. The combined genotypic and phenotypic data showed that all six strains form distinct centres of taxonomic variation within the genus Streptomyces. The following novel species are proposed to accommodate the strains: Streptomyces aldersoniae sp. nov. (type strain DSM 41909T =NRRL 18513T), Streptomyces angustmyceticus sp. nov., comb. nov. (type strain DSM 41683T=NRRL B-2347T), Streptomyces ascomycinicus sp. nov. (type strain DSM 40822T =NBRC 13981T), Streptomyces decoyicus sp. nov., comb. nov. (type strain DSM 41427T =NRRL 2666T), Streptomyces milbemycinicus sp. nov. (type strain DSM 41911T =NRRL 5739T) and Streptomyces wellingtoniae sp. nov. (type strain DSM 40632T =NRRL B-1503T).

scholarly journals 16S rRNA gene sequence based identification of Vibrio spp. in shrimp and tilapia hatcheries of Bangladesh

2017 ◽  
Vol 26 (1) ◽  
pp. 45-58 ◽  
Author(s):  
Md Abdur Razzak Hasan ◽  
Mohammad Anwar Siddique ◽  
Mahmud Hasan ◽  
M Anwar Hossain ◽  
Mohammad Shamsur Rahman
Keyword(s):  
16S Rrna ◽  
Vibrio Vulnificus ◽  
Restriction Analysis ◽  
Aquatic Organisms ◽  
Poor Quality ◽  
Vibrio Species ◽  
Economic Losses ◽  
Rrna Gene ◽  
Aeromonas Veronii ◽  
Vibrio Spp

Presence of Vibrio spp., one of the deadliest fish and shrimp pathogens in aquaculture facilities worldwide for which hatchery owners often suffer hectic economic losses, were identified in shrimp and tilapia hatcheries of Cox’s Bazar and Mymensingh, Bangladesh. Thirty seven Vibrio isolates, selected on the basis of their morphological dissimilarities in thiosulphate citrate bile salt sucrose agar (TCBS) plate, were subjected to amplified 16S ribosomal DNA restriction analysis (ARDRA) by using AluI restriction enzyme following their DNA extraction and amplification of 16S rRNA (1450 bp). From this analysis, representative isolates of 8 ARDRA groups, named as ARH 1 to ARH 8, of different band patterns were sequenced and identified as Vibrio alginolyticus, Aeromonas veronii, A. hydrophila, Vibrio vulnificus, V. cholerae, Edwardsiella hoshinae, Bacillus methylotrophicus and Aeromonas veronii, respectively. Vibrio species identified in this study are pathogenic for human and aquatic organisms, and were found only in shrimp hatchery with the dominance of V. alginolyticus. Findings of this study indicate the poor quality of water treatment and management of the hatchery. It was also observed that all the three Vibrio species were present in the Artemia rearing tank which indicates the possible source of pathogens. Dhaka Univ. J. Biol. Sci. 26(1): 45-58, 2017 (January)

Protein-induced conformational changes in 16S rRNA during assembly of the Escherichia Coli 30S ribosomal subunit: A STEM study

1987 ◽  
Vol 45 ◽  
pp. 910-911
Author(s):  
M. Boublik ◽  
V. Mandiyan ◽  
J.F. Hainfeld ◽  
J.S. Wall
Keyword(s):  
16S Rrna ◽  
Conformational Changes ◽  
Ribosomal Proteins ◽  
Structural Changes ◽  
Ribosomal Subunit ◽  
Compact Structure ◽  
E Coli ◽  
Freeze Dried ◽  
16S Rna ◽  
30S Subunit

The aim of this study is to understand the mechanism of 16S rRNA folding into the compact structure of the small 30S subunit of E. coli ribosome. The assembly of the 30S E. coli ribosomal subunit is a sequence of specific interactions of 16S rRNA with 21 ribosomal proteins (S1-S21). Using dedicated high resolution STEM we have monitored structural changes induced in 16S rRNA by the proteins S4, S8, S15 and S20 which are involved in the initial steps of 30S subunit assembly. S4 is the first protein to bind directly and stoichiometrically to 16S rRNA. Direct binding also occurs individually between 16S RNA and S8 and S15. However, binding of S20 requires the presence of S4 and S8. The RNA-protein complexes are prepared by the standard reconstitution procedure, dialyzed against 60 mM KCl, 2 mM Mg(OAc)2, 10 mM-Hepes-KOH pH 7.5 (Buffer A), freeze-dried and observed unstained in dark field at -160°.

Imaging of ribosomal RNA-protein interactions by dark-field STEM

1989 ◽  
Vol 47 ◽  
pp. 250-251
Author(s):  
M. Boublik ◽  
V. Mandiyan ◽  
S. Tumminia ◽  
J.F. Hainfeld ◽  
J.S. Wall
Keyword(s):  
Nucleic Acid ◽  
16S Rrna ◽  
Dark Field ◽  
Radius Of Gyration ◽  
Central Domain ◽  
The Core ◽  
16S Rna ◽  
30S Subunit ◽  
Core Particles

Success in protein-free deposition of native nucleic acid molecules from solutions of selected ionic conditions prompted attempts for high resolution imaging of nucleic acid interactions with proteins, not attainable by conventional EM. Since the nucleic acid molecules can be visualized in the dark-field STEM mode without contrasting by heavy atoms, the established linearity between scattering cross-section and molecular weight can be applied to the determination of their molecular mass (M) linear density (M/L), mass distribution and radius of gyration (RG). Determination of these parameters promotes electron microscopic imaging of biological macromolecules by STEM to a quantitative analytical level. This technique is applied to study the mechanism of 16S rRNA folding during the assembly process of the 30S ribosomal subunit of E. coli. The sequential addition of protein S4 which binds to the 5'end of the 16S rRNA and S8 and S15 which bind to the central domain of the molecule leads to a corresponding increase of mass and increased coiling of the 16S rRNA in the core particles. This increased compactness is evident from the decrease in RG values from 114Å to 91Å (in “ribosomal” buffer consisting of 10 mM Hepes pH 7.6, 60 mM KCl, 2 m Mg(OAc)2, 1 mM DTT). The binding of S20, S17 and S7 which interact with the 5'domain, the central domain and the 3'domain, respectively, continues the trend of mass increase. However, the RG values of the core particles exhibit a reverse trend, an increase to 108Å. In addition, the binding of S7 leads to the formation of a globular mass cluster with a diameter of about 115Å and a mass of ∽300 kDa. The rest of the mass, about 330 kDa, remains loosely coiled giving the particle a “medusa-like” appearance. These results provide direct evidence that 16S RNA undergoes significant structural reorganization during the 30S subunit assembly and show that its interactions with the six primary binding proteins are not sufficient for 16S rRNA coiling into particles resembling the native 30S subunit, contrary to what has been reported in the literature.

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