Use of an oncology sperm bank: a Canadian experience

Background: We performed a retrospective chart review in 2006to review oncology patients’ use of banked semen samples infertility treatments at a tertiary care centre.Methods: From 2002 to 2005, 367 oncology patients bankedsemen. During the same period, 31 patients used banked samplesin 48 treatment cycles. Samples were used for intrauterine insemination(IUI) in 28 cycles and for in vitro fertilization (IVF) withor without intracytoplasmic sperm injection (ICSI) in 20 cycles.Results: Pregnancy rates per cycle were 21% for IUI and 50%for IVF with or without ICSI. Overall, 16 of the 31 couples achieveda pregnancy with assisted reproductive technologies (52%).Conclusion: This data indicates high pregnancy success rates withthe use of banked semen samples from men with cancer.Contexte : Une revue rétrospective de dossiers a été effectuée afind’examiner le recours à des échantillons de sperme provenantde patients cancéreux pour le traitement de l’infertilité dans uncentre de soins tertiaires.Méthodes : Entre 2002 et 2005, des échantillons de spermeprovenant d’un total de 367 patients atteints de cancer ont été misen banque. Durant la même période, 31 patientes ont utilisé ceséchantillons au cours de 48 cycles de traitement. Les échantillonsont été utilisés pour insémination intra-utérine (IIU) lors de28 cycles et pour fertilisation in vitro (FIV) avec ou sans injectionintracytoplasmique de sperme (ICSI) lors de 20 cycles.Résultats : Le taux de grossesse par cycle était de 21 % avecl’IIU et de 50 % pour la FIV avec ou sans ICSI. Au total, 16 des31 patientes sont devenues enceintes (52 %).Conclusion : Ces données montrent des taux élevés de grossesseobtenus par l’utilisation d’échantillons de sperme provenantd’hommes atteints de cancer.

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Follicular Metabolites-Assisted Clinical Evaluation of IVF/ICSI Outcomes

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/9999659 ◽

2021 ◽

Vol 2021 ◽

pp. 1-13

Author(s):

Bing Qu ◽

Yunhe Xiong ◽

Xiaofan Yu ◽

Jinli Ding ◽

Jing Weng ◽

...

Keyword(s):

Assisted Reproductive Technologies ◽

Sperm Quality ◽

Public Health Problem ◽

Decisive Role ◽

Reproductive Technologies ◽

Sperm Parameters ◽

Success Rates ◽

Vitro Fertilization ◽

Icsi Outcomes

As infertility became a significant public health problem, assisted reproductive technologies (ARTs) were introduced. However, the fertilization rate of in vitro fertilization (IVF) per cycle varied, and patients needed to repeat IVF or change to intracytoplasmic sperm injection (ICSI). Here, 75 couples suffering from female fallopian tubal blockage (tubal group) and 42 spouses beset by male abnormal sperm status (dysspermia group) were recruited. We comprehensively explored the relationship among couples’ clinical factors, follicular metabolites, and IVF/ICSI stepwise outcomes. IVF/ICSI outcomes were affected by follicular metabolites and physical status in both women and men, regardless of which side infertility came from. Particularly, in the tubal group, the energy supporting pathways—glycolysis and pyruvate metabolism—were most essential in follicles, and IVF/ICSI outcomes were also related to sperm parameters. However, in the dysspermia group, in addition to sperm conditions, oocyte quality acted as a compensation for poor sperm quality, for which aminoacyl-tRNA biosynthesis and the related supporting metabolism were critical in the follicular environment, and ultimately played a decisive role in IVF/ICSI outcomes. The respective logistic regression models in combination with selective male sperm parameters, estradiol (E2), follicular alanine, glutamine, glycoprotein, lipid, and acetic acid, were constructed to predict IVF or ICSI outcomes. No matter which sex infertility comes from, factors from both men and women should be considered. The current study provides a feasible option for pre-IVF evaluation, as well as guidance for follow-up clinical intervention to improve IVF/ICSI success rates.

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Reproductive technologies, female infertility, and the risk of imprinting-related disorders

Clinical Epigenetics ◽

10.1186/s13148-020-00986-3 ◽

2020 ◽

Vol 12 (1) ◽

Author(s):

Patricia Fauque ◽

Jacques De Mouzon ◽

Aviva Devaux ◽

Sylvie Epelboin ◽

Marie-José Gervoise-Boyer ◽

...

Keyword(s):

Molecular Mechanisms ◽

Assisted Reproductive Technologies ◽

Intrauterine Insemination ◽

Reproductive Technologies ◽

Female Infertility ◽

Global Risk ◽

Vitro Fertilization ◽

Increased Risk ◽

Genomic Imprints

Abstract Background Epidemiological studies suggest that singletons born from assisted reproductive technologies (ART) have a high risk of adverse perinatal outcomes, specifically for imprinting disorders. Because ART processes take place at times when epigenetic reprogramming/imprinting are occurring, there is concern that ART can affect genomic imprints. However, little is currently known about the risk of imprinting defects according to the type of ART or the type of underlying female infertility. From the French national health database, a cohort of 3,501,495 singletons born over a 5-year period (2013–2017) following fresh embryo or frozen embryo transfers (fresh-ET or FET from in vitro fertilization), intrauterine insemination, or natural conception was followed up to early childhood. Based on clinical features, several syndromes/diseases involving imprinted genes were monitored. The effects of ART conception and the underlying cause of female infertility were assessed. Results Compared with infants conceived naturally, children born after fresh-ET had a higher prevalence of imprinting-related diseases, with an aOR of 1.43 [95% CI 1.13–1.81, p = 0.003]. Namely, we observed an increased risk of neonatal diabetes mellitus (1.96 aOR [95% CI 1.43–2.70], p < 0.001). There was an overall independent increase in risk of imprinting diseases for children with mothers diagnosed with endometriosis (1.38 aOR [95% CI 1.06–1.80], p = 0.02). Young and advanced maternal age, primiparity, obesity, smoking, and history of high blood pressure or diabetes were also associated with high global risk. Conclusions This prospective epidemiological study showed that the risk of clinically diagnosed imprinting-related diseases is increased in children conceived after fresh embryo transfers or from mothers with endometriosis. The increased perturbations in genomic imprinting could be caused by controlled ovarian hyperstimulation and potentially endometriosis through the impairment of endometrial receptivity and placentation, leading to epigenetic feto-placental changes. Further studies are now needed to improve understanding of the underlying molecular mechanisms (i.e. genetic or epigenetic causes).

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Endometriosis-Related Infertility: The Role of the Assisted Reproductive Technologies

BioMed Research International ◽

10.1155/2015/482959 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 24

Author(s):

Eric S. Surrey

Keyword(s):

Assisted Reproductive Technologies ◽

Large Body ◽

Ovarian Response ◽

Reproductive Technologies ◽

Invasive Disease ◽

Definitive Treatment ◽

Success Rates ◽

Vitro Fertilization ◽

Ovarian Endometriomas

The assisted reproductive technologies, particularly in vitro fertilization (IVF), represent the most efficient and successful means of overcoming infertility associated with endometriosis. Although older studies suggest that IVF outcomes are compromised in endometriosis patients, more contemporary reports show no differences compared to controls. The exception may be evidence of poorer outcomes and diminished ovarian response in women with advanced disease, particularly those with significant ovarian involvement or prior ovarian surgery. Prolonged pre-IVF cycle suppressive medical therapy, particularly gonadotropin releasing hormone agonists, appears to improve success rates in a subset of endometriosis patients. However, as of yet, there is no diagnostic marker to specifically identify those who would most benefit from this approach. Pre-IVF cycle surgical resection of nonovarian disease has not been consistently shown to improve outcomes with the possible exception of resection of deeply invasive disease, although the data is limited. Precycle resection of ovarian endometriomas does not have benefit and should only be performed for gynecologic indications. Indeed, there is a large body of evidence to suggest that this procedure may have a deleterious impact on ovarian reserve and response. A dearth of appropriately designed trials makes development of definitive treatment paradigms challenging.

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Clinical significance of sperm morphology in male infertility treatment

Experimental and Сlinical Urology ◽

10.29188/2222-8543-2021-14-3-127-132 ◽

2021 ◽

Vol 14 (3) ◽

pp. 127-132

Author(s):

Yu.V. Olefir ◽

◽

D.M. Monakov ◽

◽

Keyword(s):

Male Infertility ◽

Clinical Significance ◽

Sperm Morphology ◽

Assisted Reproductive Technologies ◽

Intrauterine Insemination ◽

Reproductive Technologies ◽

Vitro Fertilization ◽

Normal Sperm ◽

Starting Point

Introduction. Sperm morphology is one of the most common tests in fertility practice. The interlaboratory variability is the main drawback of the method. The clinical significance of sperm morphology in assisted reproductive technologies is controversial. The aim of the review is to address this question. Materials and methods. The search of relevant publications was carried out in PubMed and e-Library databases using the keywords «male infertility», «sperm morphology», «teratozoospermia», «IUI», «IVF», «ICSI». Conference abstracts and dissertations were excluded from analysis and 56 publications were included in this literature review. Results. The small numbers of studies were evaluated to the effect of teratozoospermia on the likelihood of natural pregnancy. The pregnancy rate was higher in the group of couples with normozoospermia. In the couples with severe teratozoospermia pregnancies rate was also detected. The most studies did not reveal a statistically significant effect of tertozoospermia on the frequency of pregnancy during intrauterine insemination. The data about the influence of sperm morphology on in vitro fertilization are contradictory. Early studies showed a positive correlation between normal sperm morphology and frequency of conception, but these results were not confirmed in further studies. The most studies have not been revealed the correlation between normal sperm morphology male fertility status, clinical and live birth rate. Discussion. To date the spermatozoa mofophology studying remains the «starting point» ofa man's examination for infertility. However, the data available do not confirm its role in choosing the method ofassisted reproductive technologies or predicting their results. Conclusions. To date the role of sperm morphology on conceive and pregnancy frequencies in ART use is controversal. The data available does not confirm the value of this test as a proxy of higher pregnancy and birth of healthy child probability. The further studies are required to address this question.

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Future developments in assisted reproduction in humans

Reproduction ◽

10.1530/rep.0.1230171 ◽

2002 ◽

pp. 171-183 ◽

Cited By ~ 27

Author(s):

K Hardy ◽

C Wright ◽

S Rice ◽

M Tachataki ◽

R Roberts ◽

...

Keyword(s):

Assisted Reproduction ◽

Assisted Reproductive Technologies ◽

Reproductive Technologies ◽

Early Embryo ◽

Embryo Biopsy ◽

Screening Methods ◽

Success Rates ◽

Aneuploidy Screening ◽

Vitro Fertilization

The advent of human in vitro fertilization (IVF) over 30 years ago has made the oocyte and preimplantation embryo uniquely accessible. This accessibility has given rise to new micromanipulation techniques, such as intracytoplasmic sperm injection for treatment of male infertility, as well as embryo biopsy for preimplantation diagnosis of both genetic disease and aneuploidy, a major cause of early embryo demise and miscarriage. In the UK, average pregnancy rates after IVF and embryo transfer are < 25%, even after transfer of several embryos. Unfortunately, a third of these pregnancies involve multiple gestations. Research is currently focusing on methods to improve IVF success rates while reducing twin and triplet pregnancies and their associated increased morbidity and mortality. One approach is to develop screening methods to identify the most viable embryos, so that transfer of fewer healthy embryos will result in a higher proportion of singleton pregnancies. Screening methods include optimizing culture conditions for prolonged culture and selection of viable blastocysts for transfer, or embryo biopsy and aneuploidy screening. Assisted reproduction is also increasingly important in other branches of medicine: survival rates for cancer sufferers are improving continually and there is now a significant need for approaches to preserve fertility after sterilizing chemo-and radiotherapy treatment. Techniques for cryopreserving male and female gametes or gonadal tissue are being developed, although systems to grow and mature these gametes are in their infancy. Finally, there are also concerns regarding the safety of these new assisted reproductive technologies.

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Reproductive Technologies

Anthropology ◽

10.1093/obo/9780199766567-0200 ◽

2019 ◽

Author(s):

Amy Speier ◽

Caridad Zamarripa

Keyword(s):

Assisted Reproductive Technologies ◽

Intrauterine Insemination ◽

Petri Dish ◽

Reproductive Technologies ◽

Human Reproduction ◽

Vitro Fertilization ◽

Gay Fathers ◽

The People ◽

Future Child

Reproductive technologies are those technologies that aid in animal and human reproduction. Assisted reproductive technologies (ARTs) are more narrowly defined as those technologies that help people suffering from social or bodily infertility create a family. Socially infertile includes single women and men as well as homosexual couples who rely on donated gametes for the creation of a future child. Intrauterine Insemination (IUI) is usually the first step taken by couples having trouble conceiving. The most general type of reproduction technology is in vitro fertilization (IVF), which means that the egg is retrieved from a woman’s uterus and sperm is introduced to these eggs in a petri dish. In the case of male infertility, intracytoplasmic sperm injections (ICSI) may be employed, which means that sperm are injected directly into the egg. IVF may include the use of donated sperm, oocytes, and embryos. In addition to gamete donation, surrogacy may be employed in cases where an intended mother or intended gay fathers cannot carry a pregnancy to term. In addition to being used to create families, contraception is also considered a reproductive technology. Anthropologists have been conducting ethnographic analyses of reproductive technologies by studying the people intimately engaged with these varying technologies. Scholarship revolves around major questions about markets and gift exchange, kinship, and how our understandings of family have shifted with the advent of reproductive technologies, as well as globalization and the ways in which bodies, people, and technologies traverse the globe.

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Study of the obstetrics and perinatal outcome of in vitro fertilization pregnancies at tertiary care centre

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20212152 ◽

2021 ◽

Vol 10 (6) ◽

pp. 2225

Author(s):

Geetha D. Balsarkar ◽

Shraddha P. Pol

Keyword(s):

Birth Weight ◽

Assisted Reproductive Technologies ◽

Meta Analysis ◽

Statistical Significance ◽

Tertiary Care ◽

Reproductive Technologies ◽

Multiple Gestation ◽

Tertiary Care Centre ◽

Vitro Fertilization

Background: Assisted reproductive techniques (ART) has been a boon to the unfortunate. However, the outcomes aren’t the same to all. The study has observed the outcomes in IVF conceived patients. The pregnant women were followed-up throughout their antenatal and post-natal (7 days) period and an appropriate meta-analysis was made.Methods: This is an Observational Prospective study. It was conducted at an IVF center of tertiary hospital. With valid written informed consents 96 patients were recruited and observed for18 months. Data collected by Universal sampling method, analyzed and interpreted.Results: A total 65.6% belonged above 30 years, 67.7% were primigravida and 7.3% had history of previous ectopic pregnancy. In 23.75% multifetal pregnancy was significant and 20.63% had OS tightening done. Most common reason for opting IVF was multiple failed IUI (71.6%). More than 40% were delivered by caesarean section. 91.1% of babies cried immediately after birth and 50.4% babies required NICU admission.13.4% babies had neonatal death and 3.1% were IUFD. 58.30% pregnancies were singleton. Mean birth weight was 1.87 Kg and 72.6% children were born low birth weight. Statistical significance (p<0.001) was found in between no. of foetuses delivered and preterm delivery. 56.3% birth were preterm birth. PIH and GDM complication is most common in multiple gestations observed in 10.7% of pregnancies.Conclusions: Assisted Reproductive technologies has helped the infertile couples. Outcomes could be unfruitful in few, while others may have a complicated antenatal period. Preterm deliveries are more common in multiple gestation thus complementing the lower birth weights. Comorbidities significantly affected obstetric outcome.

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Thickness of endometrium: predictor of the effectiveness of IVF/ICSI programs (literature review)

GYNECOLOGY ◽

10.26442/2079-5696_20.1.113-116 ◽

2018 ◽

Vol 20 (1) ◽

pp. 113-116

Author(s):

L A Bagdasaryan ◽

I E Korneyeva

Keyword(s):

Assisted Reproductive Technologies ◽

Endometrial Thickness ◽

Molecular Genetic ◽

Uterine Cavity ◽

Reproductive Technologies ◽

Genetic Characteristics ◽

Vitro Fertilization ◽

Need To Evaluate ◽

The Relationship

The aim of the study is to systematically analyze the data available in the modern literature on the relationship between endometrial thickness and the frequency of pregnancy in the program of assisted reproductive technologies (ART). Materials and methods. The review includes data from foreign and domestic articles found in PubMed on this topic. Results. The article presents data on the relationship between the thickness of the endometrium and the frequency of pregnancy in ART programs. The greatest number of studies is devoted to the evaluation of the relationship between the thickness of the endometrium and the frequency of pregnancy on the day of the ovulation trigger. Data are presented on the existence of a correlation between the thickness of the endometrium measured on the day of the ovulation trigger and the frequency of clinical pregnancy, as well as data on the need to evaluate the structure of the endometrium and the state of subendometric blood flow. The importance of multilayered (three-layered) endometrium as a prognostic marker of success in in vitro fertilization/intracytoplasmic sperm injection programs in the ovum is emphasized. The conclusion. The thickness of the endometrium can not be used as an argument for canceling the cycle or abolishing embryo transfer to the uterine cavity. Further studies in this direction are needed with a study of the morphological and molecular genetic characteristics of the endometrium, which in the future will allow us to evaluate the relationship between the thickness of the endometrium and the probability of pregnancy.

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Legal restrictions on pre-implantation genetic diagnostics in the framework of in vitro fertilization procedure: foreign experience and Russian perspective

10.21516/2413-1458-2019-18-2-162-168 ◽

2019 ◽

Author(s):

N.A. Altinnik , S.S. Zenin , V.V. Komarova et all

Keyword(s):

In Vitro Fertilization ◽

Assisted Reproductive Technologies ◽

Genetic Diseases ◽

Genetic Diagnosis ◽

Reproductive Technologies ◽

Legal Regulation ◽

Genetic Diagnostics ◽

Vitro Fertilization ◽

Medical Indications

The article discusses the factors that determine the content of the legal limitations of pre-implantation genetic diagnosis in the framework of the in vitro fertilization procedure, taking into account international experience and modern domestic regulatory legal regulation of the field of assisted reproductive technologies. The authors substantiates the conclusion that it is necessary to legislate a list of medical indications for preimplantation genetic diagnosis, as well as the categories of hereditary or other genetic diseases diagnosed in the framework of this procedure.

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Prevalence of pathogenic copy number variants among children conceived by donor oocyte

Scientific Reports ◽

10.1038/s41598-021-86242-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Sandra Monfort ◽

Carmen Orellana ◽

Silvestre Oltra ◽

Mónica Rosello ◽

Alfonso Caro-Llopis ◽

...

Keyword(s):

In Vitro Fertilization ◽

Copy Number ◽

Assisted Reproductive Technologies ◽

Copy Number Variants ◽

Reproductive Technologies ◽

Significant Excess ◽

Vitro Fertilization ◽

Donor Oocyte ◽

Increased Risk

AbstractDevelopment of assisted reproductive technologies to address infertility has favored the birth of many children in the last years. The majority of children born with these treatments are healthy, but some concerns remain on the safety of these medical procedures. We have retrospectively analyzed both the fertilization method and the microarray results in all those children born between 2010 and 2019 with multiple congenital anomalies, developmental delay and/or autistic spectrum disorder (n = 486) referred for array study in our center. This analysis showed a significant excess of pathogenic copy number variants among those patients conceived after in vitro fertilization with donor oocyte with respect to those patients conceived by natural fertilization (p = 0.0001). On the other hand, no significant excess of pathogenic copy number variants was observed among patients born by autologous oocyte in vitro fertilization. Further studies are necessary to confirm these results and in order to identify the factors that may contribute to an increased risk of genomic rearrangements, as well as consider the screening for genomic alterations after oocyte donation in prenatal diagnosis.

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