Revisiting Therapeutic Potentials of Ethanolic Extract of Curcuma longa L. rhizomes to Evaluate wound Healing Progression upon Topical Application of its Ointment

ABSTRACT Remek daging (Hemigraphis colorata W.Bull) have been studied and used traditionally for wound healing. This study aimed to determine the effect of topical application of remek daging leaves ethanolic extract 70% on the burn wound. The animals used for this study were 30 rats, divided into five groups, namely 20, 10, 5% remek daging extract ointment, negative control (vaseline flavum), and positive control (silver sulfadiazine 1%). Histology observations were held on days 3, 7, and 14 after burn wound induction. Histological observations showed an increase number of macrophages, fibroblasts, collagen density, and re-epithelialization in the extract ointment group significantly compare to the negative control (p <0.05). The application of ointment extract 20% to the rats showed comparable results to silver sulfadiazine 1% (p> 0.05). It can be concluded that remek daging ointment extract can accelerate the healing of burn wounds with the best results at a concentration of 20%. Keywords: Hemigraphis colorata, burns, macrophages, fibroblasts, collagen. ABSTRAK Remek daging (Hemigraphis colorata W.Bull) telah diteliti dan digunakan untuk penyembuhan luka secara tradisional. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol 70% daun remek daging secara topikal pada luka bakar tikus putih. Hewan yang digunakan adalah 30 ekor tikus yang dibagi menjadi 5 kelompok yaitu kelompok salep ekstrak daun remek daging 20, 10, 5% (%b/b), kontrol negatif (vaselin flavum) dan kontrol positif (silver sulfadiazine 1% (%b/b)). Pengamatan secara histologi dilakukan pada hari ke 3, 7 dan 14 setelah induksi luka bakar. Pengamatan histologi menunjukkan peningkatan jumlah makrofag, jumlah fibroblas, kepadatan kolagen dan ketebalan re-epitelisasi pada kelompok salep ekstrak daun remek daging secara signifikan dibandingkan kontrol negatif (p<0,05). Tikus yang diberikan perlakuan salep ekstrak 20% menunjukkan hasil sebanding dengan silver sulfadiazin 1% (p>0,05). Dapat disimpulkan bahwa salep ekstrak daun remek daging dapat mempercepat penyembuhan luka bakar dengan hasil terbaik pada konsentrasi 20%. Kata kunci: Hemigraphis colorata, luka bakar, makrofag, fibroblas, kolagen.

Download Full-text

Effect of Curcuma longa Crude Extract, Curcumin and Nano Curcumin-based Gel Topical Administration on Excised Skin Wound Healing in Swiss-Webster Mice

Biomedical & Pharmacology Journal ◽

10.13005/bpj/2165 ◽

2021 ◽

Vol 14 (02) ◽

pp. 635-641

Author(s):

Afiat Berbudi ◽

Ali Akbar Fadlillah ◽

Merry Afni ◽

Nur Atik

Keyword(s):

Wound Healing ◽

Topical Application ◽

Curcuma Longa ◽

Area Under The Curve ◽

Topical Administration ◽

Treated Group ◽

Healing Time ◽

Control Group ◽

Skin Wound Healing ◽

Significant Difference

Patients suffering from skin wounds often have their life heavily affected economically and socially. In general, wound healing consists of four different sequentially and overlapping phases. Interrupting those phases results in extending the healing time. Turmeric (Curcuma longa) is widely used by Indonesians to treat wounds, with curcumin as an active substance, which is proven to have various effects on improving wound healing. Therefore, this study aims to determine how the topical application of turmeric gel on affects the acceleration of wound closure. Analytical laboratory experiments were conducted on 24 male Swiss-Webster mice. Subjects were divided into four groups according to where the excision wounds were made on the back, and observed on days 0, 3, 6, 9, 12, and 15 post wound induction. The wound healing acceleration was observed after 3 (p = 0.0003), 6 (p = 0.0085), and 9 (p = 0.0023) days in C. longa-treated mice compared to the control group. However, there was no significant difference found on days 12 and 15 after wounding. In addition, the area under the curve analysis shows significant difference between the C. longa-treated group compared to the control, but not with the group treated with neither curcumin nor nano curcumin gel. In conclusion, topical application of 3% C. longa extract gel improves excised skin healing.

Download Full-text

Effect of Diabetes Condition on Topical Treatment of Binahong Leaf Fraction in Wound Healing Process

Majalah Obat Tradisional ◽

10.22146/tradmedj.27921 ◽

2017 ◽

Vol 22 (2) ◽

pp. 103

Author(s):

Kintoko Kintoko ◽

Hanifah Karimatulhajj ◽

Trie Yuni Elfasyari ◽

Ersi Arviana Ihsan ◽

Teguh Adiyas Putra ◽

...

Keyword(s):

Wound Healing ◽

Blood Glucose ◽

Topical Application ◽

Healing Process ◽

Ethanolic Extract ◽

Positive Control ◽

Diabetic Rats ◽

Wound Healing Process ◽

Diabetic Ulcers ◽

Control Diabetes

Diabetes mellitus (DM) is a metabolic disease which is becoming the first number of health problem in Indonesia, based on the results of the Basic Health Research of Ministry of Health in 2013. One of the diabetes complications affected by high levels of blood glucose is diabetic ulcers wich 85% the number of cases was overed by amputation as the result of improper handling. Herbal treatments could be an alternative treatment of diabetic ulcers, one of them is binahong plant. The results of previous studies have shown the ability of ethanolic extract of binahong leaf in accelerating wound healing in diabetic rats. This study would be a continued study to test five kinds of binahong leaf fractions (FDB) with gradual solvent polarity in accelerating wound healing in diabetic ulcer. Wound healing parameters observed were percentage of wound healing from the wound diameter contraction. The test begins with ethanolic extract of binahong leaf fractionation to produce fractions of hexane (FHDB), chloroform (FKDB), ethyl acetate (FEADB), and ethanol (FEDB). Water fraction (FADB) was obtained from ethanolic leaf extract residues of binahong leaf extraction. Each fraction activity was tested topically twice daily on dorsal of test animals that created wounds using a punch biopsy 5 mm diameters. Grouping of test animals were divided into 13 groups with normal control group (non diabetes), negative control (diabetes + topical application of biocream®), positive control (diabetes + topical application of madecassol), and 10 diabetes groups with topical application of 5 kinds of binahong leaf fractions with each fractions consist of a concentrations of 5% and 10% with biocream® as a vehicle. Diabetic parameters measured include blood glucose levels (KGD) and weight lost percentage (PB%) in day 1 and 10 during a 10-day treatment. The results showed the influence of KGD in the condition of diabetes on wound healing rats diabetic ulcers which topically treated with binahong leaf fractions. FADB 10% has significant differences potention in wound healing process in diabetic rats macroscopically that accelerates wound contraction compared with negative and positive control groups (p<0,05).

Download Full-text

Bacterial Cellulose Membrane Containing Epilobium angustifolium L. Extract as a Promising Material for the Topical Delivery of Antioxidants to the Skin

International Journal of Molecular Sciences ◽

10.3390/ijms22126269 ◽

2021 ◽

Vol 22 (12) ◽

pp. 6269

Author(s):

Anna Nowak ◽

Paula Ossowicz-Rupniewska ◽

Rafał Rakoczy ◽

Maciej Konopacki ◽

Magdalena Perużyńska ◽

...

Keyword(s):

Bacterial Cellulose ◽

Topical Application ◽

Phenolic Acid ◽

Antioxidant Properties ◽

Ethanolic Extract ◽

Cellulose Membrane ◽

Hydroxybenzoic Acid ◽

Epilobium Angustifolium ◽

Bacterial Cellulose Membrane

Bacterial cellulose membranes (BCs) are becoming useful as a drug delivery system to the skin. However, there are very few reports on their application of plant substances to the skin. Komagataeibacter xylinus was used for the production of bacterial cellulose (BC). The BC containing 5% and 10% ethanolic extract of Epilobium angustifolium (FEE) (BC-5%FEE and BC-10%FEE, respectively) were prepared. Their mechanical, structural, and antioxidant properties, as well as phenolic acid content, were evaluated. The bioavailability of BC-FESs using mouse L929 fibroblasts as model cells was tested. Moreover, in vitro penetration through the pigskin of the selected phenolic acids contained in FEE and their accumulation in the skin after topical application of BC-FEEs was examined. The BC-FEEs were characterized by antioxidant activity. The BC-5% FEE showed relatively low toxicity to healthy mouse fibroblasts. Gallic acid (GA), chlorogenic acid (ChA), 3,4-dihydroxybenzoic acid (3,4-DHB), 4-hydroxybenzoic acid (4-HB), 3-hydroxybenzoic acid (3-HB), and caffeic acid (CA) found in FEE were also identified in the membranes. After topical application of the membranes to the pigskin penetration of some phenolic acid and other antioxidants through the skin as well as their accumulation in the skin was observed. The bacterial cellulose membrane loaded by plant extract may be an interesting solution for topical antioxidant delivery to the skin.

Download Full-text

Topical Application of Ozonated Oils for the Treatment of MRSA Skin Infection in an Animal Model of Infected Ulcer

Biology ◽

10.3390/biology10050372 ◽

2021 ◽

Vol 10 (5) ◽

pp. 372

Author(s):

Vanessa Silva ◽

Cecília Peirone ◽

Rosa Capita ◽

Carlos Alonso-Calleja ◽

José A Marques-Magallanes ◽

...

Keyword(s):

Wound Healing ◽

Animal Model ◽

Topical Application ◽

Early Stage ◽

Bacterial Load ◽

Ozone Treatment ◽

Diabetic Patients ◽

Colony Forming Units ◽

Group 2 ◽

Epidermal Regeneration

Diabetic foot ulcers are a common cause of morbidity in diabetic patients. One of the main pathogens found in these ulcers is methicillin-resistant Staphylococcus aureus (MRSA). MRSA often carries resistance to several classes of antibiotics and their infections are becoming harder to treat. Therefore, new alternatives are urgently needed. Thus, this study aimed to investigate the capacity of topical ozonated oil application on the treatment of early-stage skin infected with MRSA in an animal model. Ozonated oil was prepared from a mixture of oils subjected to a gas stream of O2/O3 mixture. Sixteen Wistar rats were inoculated by an intradermic injection of MRSA suspension, producing an abscess lesion. After 3 days, the skin epidermis was removed to open the wound. Group 1 received an application of oil mixture without ozone treatment and Group 2 received an application of ozonated oil. After the treatment period, skin was collected, colony-forming units (CFU) of bacteria were quantified and the histological analysis of the skin was carried out. Skin samples from the control 1 and 2 had a bacterial load was of 1.1 × 105 and 5.7 × 103 CFU/mL, respectively. Group 2 showed better wound healing from mild to moderate epidermal regeneration. Topical application of ozonated vegetable oil in MRSA-infected skin in rats showed a small reduction of the bacterial load and better wound healing.

Download Full-text

Acceleration of wound healing by topical application of honey

The American Journal of Surgery ◽

10.1016/0002-9610(83)90204-0 ◽

1983 ◽

Vol 145 (3) ◽

pp. 374-376 ◽

Cited By ~ 121

Author(s):

Arieh Bergman ◽

Joseph Yanai ◽

Jerry Weiss ◽

David Bell ◽

Menachem P. David

Keyword(s):

Wound Healing ◽

Topical Application

Download Full-text

Evaluation of Aqueous Creams Containing Ethanolic Extract of Curcuma longa (Turmeric) as Bioactive Ingredient for the Management of Wounds

International Journal of Applied Biology and Pharmaceutical Technology ◽

10.26502/ijabpt.202103 ◽

2021 ◽

Vol 12 (01) ◽

Author(s):

Kenneth Chinedu Ugoeze ◽

Majiroghene Uyoyoumaero Okpa ◽

Nkemakolam Nwachukwu ◽

Bruno Chukwuemeka Chinko ◽

Kennedy Emeka Oluigbo

Keyword(s):

Curcuma Longa ◽

Ethanolic Extract

Download Full-text

WOUND HEALING ACTIVITY OF THE WHOLE PLANT OF MOMORDICA CHARANTIA LINN. IN RATS

INDIAN DRUGS ◽

10.53879/id.55.11.11317 ◽

2018 ◽

Vol 55 (11) ◽

pp. 64-70

Author(s):

M.P Singh ◽

◽

Y.S. Sarangdevot ◽

S.S. Sisodia

Keyword(s):

Wound Healing ◽

Ethyl Acetate ◽

Petroleum Ether ◽

Ethanolic Extract ◽

Momordica Charantia ◽

Alcohol Extract ◽

Whole Plant ◽

Healing Property ◽

Aqueous Ethanolic Extract ◽

Wound Healing Activity

The aim of the present study was to explore the effect of wound healing activity of the whole plant of Momordica charantia Linn. in rats. The effect of aqueous ethanolic extract of Momordica charantia Linn. (Family; Cucurbetaceae) was evaluated on resutured incision, granuloma pouch and excision wound models in rats. The wound healing activity was measured by the rate of wound contraction, skin breaking strength, weight of the granulation tissue, and collagen content. The concentrated ethyl alcohol extract was subject to fractionation by using petroleum ether (40-60o V/V), solvent ether, ethyl acetate, butanol, butanone, ethanol and methanol in succession. All the fractions were screened for wound healing property. The results of pharmacological activity led to the conclusion that the petroleum ether fraction exhibited more significant wound healing promotion property followed by solvent ether and butanone, from all the parameters studied. Ethyl acetate, ethanol and methanol fractions were least effective.

Download Full-text