scholarly journals Effect of Diabetes Condition on Topical Treatment of Binahong Leaf Fraction in Wound Healing Process

2017 ◽  
Vol 22 (2) ◽  
pp. 103
Author(s):  
Kintoko Kintoko ◽  
Hanifah Karimatulhajj ◽  
Trie Yuni Elfasyari ◽  
Ersi Arviana Ihsan ◽  
Teguh Adiyas Putra ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Glucose ◽  
Topical Application ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Diabetic Ulcers ◽  
Control Diabetes

Diabetes mellitus (DM) is a metabolic disease which is becoming the first number of health problem in Indonesia, based on the results of the Basic Health Research of Ministry of Health in 2013. One of the diabetes complications affected by high levels of blood glucose is diabetic ulcers wich 85% the number of cases was overed by amputation as the result of improper handling. Herbal treatments could be an alternative treatment of diabetic ulcers, one of them is binahong plant. The results of previous studies have shown the ability of ethanolic extract of binahong leaf in accelerating wound healing in diabetic rats. This study would be a continued study to test five kinds of binahong leaf fractions (FDB) with gradual solvent polarity in accelerating wound healing in diabetic ulcer. Wound healing parameters observed were percentage of wound healing from the wound diameter contraction. The test begins with ethanolic extract of binahong leaf fractionation to produce fractions of hexane (FHDB), chloroform (FKDB), ethyl acetate (FEADB), and ethanol (FEDB). Water fraction (FADB) was obtained from ethanolic leaf extract residues of binahong leaf extraction. Each fraction activity was tested topically twice daily on dorsal of test animals that created wounds using a punch biopsy 5 mm diameters. Grouping of test animals were divided into 13 groups with normal control group (non diabetes), negative control (diabetes + topical application of biocream®), positive control (diabetes + topical application of madecassol), and 10 diabetes groups with topical application of 5 kinds of binahong leaf fractions with each fractions consist of a concentrations of 5% and 10% with biocream® as a vehicle. Diabetic parameters measured include blood glucose levels (KGD) and weight lost percentage (PB%) in day 1 and 10 during a 10-day treatment. The results showed the influence of KGD in the condition of diabetes on wound healing rats diabetic ulcers which topically treated with binahong leaf fractions. FADB 10% has significant differences potention in wound healing process in diabetic rats macroscopically that accelerates wound contraction compared with negative and positive control groups (p<0,05).

scholarly journals Efek aplikasi topikal gel ekstrak pandan wangi terhadap penyembuhan luka gingiva

2017 ◽  
Vol 2 (2) ◽  
pp. 53
Author(s):  
Icha Nofikasari ◽  
Afifah Rufaida ◽  
Chynintia Dewi Aqmarina ◽  
Failasofia Failasofia ◽  
Annisa Rahmi Fauzia ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessel ◽  
Topical Application ◽  
Healing Process ◽  
Punch Biopsy ◽  
Positive Control ◽  
Negative Control ◽  
Wound Healing Process ◽  
Wound Area ◽  
Pandanus Amaryllifolius

The Effect of topical application pandan extract gel on gingival wound. Post-gingivectomy wound is usually covered by periodontal dressing, which generally contains some chemical compounds to protect the wound. However, it can provide allergic effect on some patients. Pandan leaves (Pandanus amaryllifolius Roxb.) contain a number of active substances that have anti-inflammatory, antioxidant and antibacterial effect and play a role in wound healing. This study aims to determine the effect of topical application of Pandan leaf extract gel on gingival wound healing. Gingivectomy model was carried out on mandibular incisive gingival using the 2.5-mm punch biopsy. Thirty-six rats were randomly divided into 3 groups of treatment: negative control (CMC-Na), positive control (Aloclair), and 50% of pandan extract gel. The gels on each group were applied (twice in a day) to the wound area after gingivectomy. The observation of the wound healing process was also carried out on day 1, 3, 7, and 14 by making the histological preparations of gingival wound area. The number of blood vessels was observed using microscope and data was analysed using Two- Way Anova and LSD. The result showed that number of blood vessel increased on day 3 and the peak was on day 7. Anova and LSD test showed several significant differences comparison the number blood vessel between treatment and control. In conclusion, topical application Pandan leaves extract gel could accelerate gingival wound healing.ABSTRAKLuka pasca gingivektomi dibalut dengan periodontal dressing yang mengandung senyawa kimia dengan tujuan melindungi luka, namun senyawa kimia periodontal dressing yang ada di pasaran dapat menimbulkan efek alergi terhadap beberapa pasien. Daun pandan wangi (Pandanus amaryllifolius Roxb.) mengandung zat aktif yang memiliki anti inflamasi, antioksidan, dan antibakteri kemungkinan berperan dalam proses penyembuhan luka. Penelitian ini bertujuan untuk mengetahui peran gel ekstrak daun pandan wangi dalam proses penyembuhan luka pasca gingivektomi pada tikus wistar melalui pengamatan jumlah pembuluh darah. Model gingivektomi dilakukan pada gingiva incisivus mandibula dengan menggunakan punch biopsy diameter 2,5 mm. Tiga puluh enam tikus dibagi secara acak ke dalam 3 kelompok perlakuan yaitu kontrol negatif (Gel CMC-Na), kontrol positif (Aloclair), dan gel ekstrak pandan wangi 50%. Gel uji pada masing- masing kelompok diaplikasikan pada area luka pasca gingivektomi dua kali sehari. Pengamatan proses penyembuhan luka dilakukan pada hari ke 1, 3, 7, dan 14 dengan membuat preparat histologi gingiva area luka. Parameter penyembuhan luka yang diamati adalah jumlah pembuluh darah. Data jumlah pembuluh darah dianalisis dengan menggunakan uji statistik parametrik. Hasil penelitian menunjukkan jumlah pembuluh darah mengalami peningkatan mulai hari ke-3 dan mencapai puncaknya pada hari ke-7. Hasil uji two way Anova menunjukkan adanya perbedaan yang bermakna rerata jumlah pembuluh darah antar kelompok perlakuan dengan kontrol positif maupun negatif (p<0,05). Hasil uji LSD juga menunjukkan perbedaan yang bermakna perbandingan kelompok kontrol dan perlakuan pada semua hari pengamatan. Kesimpulan penelitian ini adalah gel ekstrak pandan wangi 50% dapat mempercepat proses penyembuhan luka pasca gingivektomi dengan kemampuannya meningkatkan jumlah pembuluh darah.

scholarly journals Effects of Low-Intensity Laser Irradiation on Wound Healing in Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Hui Ma ◽  
Ying-xin Li ◽  
Hong-li Chen ◽  
Mei-ling Kang ◽  
Timon Cheng-Yi Liu
Keyword(s):  
Wound Healing ◽  
Blood Glucose ◽  
Laser Irradiation ◽  
Healing Process ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Low Intensity ◽  
Intensity Laser ◽  
Later Phase

Objective.The effects of low-intensity 630 nm semiconductor laser irradiation at 3.6 J/cm2(LISL) on wound healing in diabetic rats were studied in this paper.Methods.36 diabetic rats with dorsal cutaneous excisional wounds were divided into three LISL groups and a control group randomly. The three LISL groups were irradiated with LISL at 5, 10, and 20 mW/cm2five times a week for two weeks, respectively. The process of wound healing was assessed by assessing blood glucose, calculating percentage of wound closure, histopathological evaluation, and immunohistochemical quantification.Results.Blood glucose of all groups remained at similar levels throughout the experiment. LISL could obviously promote wound contraction, fibroblasts proliferation, and collagen synthesis, alter bFGF and TGF-β1 expression, and reduce inflammatory reaction in the early and middle phases of chronic wound-healing process. However, LISL could not shorten cicatrization time, and the treatment effects were not sensitive to illuminate parameters in the later phase of the experiment.Conclusions.LISL might have auxiliary effects in the early and middle phases of wound healing in STZ-induced diabetic rats, but the reciprocity rule might not hold. The wound-healing process of early-phase diabetes rats shows typical characteristics of self-limited disease.

Use of bromelain in cutaneous wound healing in streptozocin-induced diabetic rats: an experimental model

2020 ◽  
Vol 29 (9) ◽  
pp. 488-495
Author(s):  
Amene Nikgoftar Fathi ◽  
Mohammad Hassan Sakhaie ◽  
Sepehr Babaei ◽  
Soroush Babaei ◽  
Fateme Slimabad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Wound Area ◽  
Small Vessels ◽  
Histological Sections ◽  
Therapeutic Benefits ◽  
Wide Range

Objective: To assess the effect of bromelain on different aspects of the wound healing process in type 1 diabetic rats. Method: In this study, 112 streptozocin-diabetic (type 1) male Wistar rats were euthanised; 28 each on days three, five, seven and 15, after a wound incision had been made. To estimate changes in a number of different cellular and tissue elements, histological sections were provided from all wound areas and stained with haematoxylin and eosin. Some 1.056mm2 of total wound area from all specimens were evaluated, by assessment of 4200 microscope photos provided from all histological sections, by stereological methods. A biomechanical test of each wound area was performed with an extensometer to evaluate the work-up to maximum force and maximum stress of the healed wound on day 15. Results: In the experimental groups, bromleain caused significant wound contraction and reduced granulation tissue formation by day 7 (p=0.003); increased neovasculars (new small vessels that appear in the wound area during wound healing) on days three, five and seven (p=0.001); significantly increased fibroblasts on day five but decreased by day seven (p=0.002); and significantly decreased macrophage numbers and epithelium thickness on all days of study (p=0.005). Wound strength significantly increased in experimental groups by day 15. Conclusion: Bromelain has a wide range of therapeutic benefits, but in most studies the mode of its action is not properly understood. It has been proved that bromelain has no major side effects, even after prolonged use. According to the results of this study, bromelain can be used as an effective health supplement to promote and accelerate wound healing indices, reduce inflammation and improve biomechanical parameters in diabetic wounds.

scholarly journals Ethanol extract ofCotinus coggygrialeaves accelerates wound healing process in diabetic rats

2016 ◽  
Vol 54 (11) ◽  
pp. 2732-2736 ◽  
Author(s):  
Halil Aksoy ◽  
Ali Sen ◽  
Mesut Sancar ◽  
Turgut Sekerler ◽  
Dilek Akakin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Wound Healing Process

scholarly journals Esktrak ikan haruan (Channa striata) menurunkan jumlah makrofag pada fase inflamasi proses penyembuhan luka (Extract of haruan (Channa striata) decreases macrophages count in inflammation phase of wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 6
Author(s):  
Putri Sri Hartini ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Wistar Rats ◽  
Healing Process ◽  
Positive Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Post Test ◽  
Channa Striata

Haruan is a kind of freshwater fishes commonly found in South Kalimantan. Haruan extract contains nutritional substancessuch as albumin, Zn, Fe, Cu, and unsaturated fatty acid which can help accelerate wound healing. The aim of this studywas to histopathologically assess the effect of 100%, 50%, and 25%haruan extract compared to aquadest and ibuprofenon macrophages count in inflammation phase of wistar rats’ buccal mucosa wound healing. This research was a trueexperimental with post test-only with control design. Samples used were 30 wistar rats divided into 5 groups, 100%, 50%,25% haruan extract treatment groups, ibuprofen treatment group as positive control, and aquadest treatment group asnegative control. Mean macrophages counted on day 3 of 100%, 50%, 25% haruan extract treatment groups aquadest,and ibuprofenl were 2.05, 4.4, 3.9, 3.3, 2.4 respectively. In conclusion, haruan extract had a significant effect in decreasingmacrophages count in inflammation phase of wound healing.

scholarly journals An assessment of the wound healing potential of a herbal gel containing an Azadirachta indica leaf extract

2021 ◽  
Vol 66 (No. 3) ◽  
pp. 99-109
Author(s):  
M Munir ◽  
SNH Shah ◽  
U Almas ◽  
FA Khan ◽  
A Zaidi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Leaf Extract ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Physicochemical Characteristics ◽  
Wound Healing Process ◽  
E Coli ◽  
Carbopol 940 ◽  
Gel Formulations

The objective of this study was to produce a Carbopol 940 based gel formula containing an Azadirachta indica leaf extract and evaluate its wound healing potential. The ethanolic extract was derived from the dried leaves of Azadirachta indica and was subjected to a phytochemical evaluation. Three gel formulations of Carbopol 940 containing an Azadirachta indica extract in three different concentrations, i.e., 1, 2, and 3% w/w were prepared. These gels were evaluated for their physical appearance, stability, antimicrobial activity, extrudability, skin irritability, pH, spreadability, and viscosity. The prepared formulas were stable, greenish and homogeneous. None of them showed irritation to the skin. The spreadability (g.cm/sec), viscosity (cps), and pH of all three formulations was 34.68, 53 270–65 400, and 6–7, respectively. Gel-III exhibited the highest antimicrobial potential against E. coli and P. aeruginosa with a zone of inhibition of 16.2 ± 0.6 mm and 15.6 ± 0.6 mm, respectively. It was revealed from the wound healing studies that the epithelialisation time for the Albino rabbits treated with Gel-III was 23 days. The Albino rabbits treated with Gel-I, Gel-II, a standard gel, and those with the untreated one (control), epithelialised in 27, 25, 26, and 34 days, respectively. A formulation containing 3% w/w extract showed better antimicrobial activity, physicochemical characteristics, and pharmacological parameters than the other formulations. It can be concluded that the wound healing process was faster with the gel formulation containing 3% w/w of the Azadirachta indica extract, proposing that this formulation is a promising candidate for wound healing.

scholarly journals An EGF- and Curcumin-Co-Encapsulated Nanostructured Lipid Carrier Accelerates Chronic-Wound Healing in Diabetic Rats

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4610
Author(s):  
Hye-Jin Lee ◽  
Moses Jeong ◽  
Young-Guk Na ◽  
Sung-Jin Kim ◽  
Hong-Ki Lee ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Healing Process ◽  
Double Emulsion ◽  
Average Diameter ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Nanostructured Lipid Carrier

Nanostructured lipid carriers (NLC) are capable of encapsulating hydrophilic and lipophilic drugs. The present study developed an NLC containing epidermal growth factor (EGF) and curcumin (EGF–Cur-NLC). EGF–Cur-NLC was prepared by a modified water-in-oil-in-water (w/o/w) double-emulsion method. The EGF–Cur-NLC particles showed an average diameter of 331.8 nm and a high encapsulation efficiency (81.1% and 99.4% for EGF and curcumin, respectively). In vitro cell studies were performed using two cell types, NIH 3T3 fibroblasts and HaCaT keratinocytes. The results showed no loss of bioactivity of EGF in the NLC formulation. In addition, EGF–Cur-NLC improved in vitro cell migration, which mimics the wound healing process. Finally, EGF–Cur-NLC was evaluated in a chronic wound model in diabetic rats. We found that EGF–Cur-NLC accelerated wound closure and increased the activity of antioxidant enzymes. Overall, these results reveal the potential of the NLC formulation containing EGF and curcumin to promote healing of chronic wounds.

scholarly journals Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments

2008 ◽  
Vol 53 (No. 12) ◽  
pp. 652-659 ◽  
Author(s):  
P. Gal ◽  
Kilik ◽  
R ◽  
M. Mokry ◽  
B. Vidinsky ◽  
...  
Keyword(s):  
Wound Healing ◽  
Quantitative Methods ◽  
Healing Process ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Polymorphonuclear Leucocytes ◽  
Skin Wound Healing ◽  
Simple Method

The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round full thickness skin wounds were performed on the back of male Sprague-Dawley rats. Animals were sacrificed two, six, and fourteen days after surgery. Sections were stained with hematoxylin-eosin and van Gieson. Both semi-quantitative (wound reepithelization; presence of: inflammatory cells, fibroblasts, new wessels, and collagen) and quantitative methods (polymorphonuclear leucocytes/tissue macrophages ratio, percentage of re-epithelization, area of the granulation tissue) were used to evaluate the histological changes during wound healing. As compared to the control group the wound healing process of both experimental groups was decelerated. Interestingly, wound reepithelization and angiogenesis were significantly inhibited only in the steroid rats while epithelization was accelerated in diabetic rats. In conclusion, when compared to primary sutured wound healing it can be concluded that the excisional model is more appropriate for histological assessment of the effect of various factors on wound healing. In addition, administration of corticosteroids represents simple and inexpensive model of a complex skin wound healing impairment.

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