scholarly journals Assessment in Vitro of Antibacterial Activity of Manipulated Product, on Solution Form, Obtained from Dry Extract of Leaves of Psidium guajava L.

2015 ◽  
Vol 8 (1) ◽  
pp. 64
Author(s):  
Marcos V.S. Silva ◽  
Tayara S. Frazão ◽  
Melyssa M.A.V. Ferreira ◽  
Jandir S. Sales ◽  
Denis B. Vieira ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Cytotoxic Activity ◽  
Staphylococcus Epidermidis ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Dry Extract ◽  
Agar Diffusion ◽  
Agar Diffusion Method

<p class="Default">The <em>Psidium guajava L</em>. specie is a perennial shrub, belonging to the <em>Myrtaceae</em> family and it is popularly known as guava, its leaves are used in therapy for treating various diseases. The study aims to evaluate the antibacterial activity <em>in vitro </em>of manipulated product obtained from dried extract of the leaves of <em>P. guajava </em>L. front standard bacteria ATCC and clinical isolates. The tests were conducted on bacterial samples: <em>Staphylococcus aureus </em>(ATCC 25923), <em>Pseudomonas aeruginosa </em>(ATCC 27883), <em>Escherichia coli </em>(ATCC 25922), <em>Salmonella spp</em>, <em>Acinetobacter baumannii</em>, <em>Proteus mirabilis</em>, <em>Shigella flexneri</em>, <em>Staphylococcus epidermidis</em>, <em>Staphylococcus haemolyticus</em>, <em>Streptococcus agalactiae, Streptococcus mutans</em>. Among the tests can be mentioned phytochemical of the ethanol extract (EE), microbiological control and physical-chemical analysis of the product and microbiological tests such as agar diffusion method (wells), minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), an evaluation test of hemolytic capacity of the solution and in vitro assay cytotoxic activity were performed, . The best result of the product in the agar diffusion method was front <em>Staphylococcus epidermidis</em>, while the lower MIC and MBC were front <em>Staphylococcus aureus </em>(ATCC 25923). The product showed no hemolytic activity and no cytotoxic activity at the tested concentrations. According to the test results, it is believed on the possibility of the production of a pharmaceutical formulation derived from the dry extract of <em>Psidium guajava</em>, since it showed great antibacterial activity.</p>

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 239
Author(s):  
Ratih Dyah Pertiwi ◽  
Joni Kristanto ◽  
Graha Ayu Praptiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Constituents ◽  
Optimal Concentration ◽  
Diffusion Method ◽  
Abrus Precatorius ◽  
Agar Diffusion ◽  
Medicinal Properties ◽  
Agar Diffusion Method ◽  
Gel Preparation

One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria

scholarly journals AKTIVITAS SEDIAAN GEL KUERSETIN TERHADAP Staphylococcus Epidermidis

2015 ◽  
Vol 1 (1) ◽  
pp. 59-64
Author(s):  
Rina Apriana Herslambang ◽  
Dina Rahmawanty ◽  
Mia Fitriana
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Phenolic Compound ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Sebaceous Glands ◽  
Agar Diffusion ◽  
Agar Diffusion Method

Acne is the abnormality production of sebum in the sebaceous glands which are usually caused by Staphylococcus epidermidis. Staphylococcus epidermidis one of bacterial that caused acne. Quercetin have a phenolic compound that can inhibit the growth of Staphylococcus epidermidis at 0.05% w/w. The purpose of this study was to determine the minimum inhibitory concentration of quercetin gel. HPMC was used as gel base. The concentrations of quercetin that was added to gel were 0.05% w/w, 0.15% w/w, and 0.25% w/w. The antibacterial activity of the gels was determined using agar diffusion method. The results showed that the inhibition zone on the F1, F2, and F3 were 7.83 mm , 6.53 mm , 4.56 mm and the result showed that quercetin gels significantly effected the growth of Staphylococcus epidermidis (p<0,05). The minimum inhibitory concentration of quercetin gel was 0,05% w/w with inhibition zone of 7.83 mm.

scholarly journals FORMULASI OBAT KUMUR GAMBIR DAN UJI EFEKTIVITAS TERHADAP BAKTERI Staphylococcus aureus

2020 ◽  
Vol 3 (1) ◽  
pp. 38-47
Author(s):  
Zola Efa Harnis
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Oral Cancer ◽  
Dental Health ◽  
Positive Control ◽  
Diffusion Method ◽  
Polyphenol Compounds ◽  
Activity Test ◽  
Agar Diffusion Method ◽  
Main Components

Oral hygiene and dental health are very important, there are various kinds of patogen  bacteria in the oral cavity, including the bacteria that cause thrush, Staphylococcus aureus. Gambir is the result of extraction containing polyphenol compounds. The main components contained in gambir consist of alkaloids, flavonoids, tannins and saponins. The use of gambir as a mouthwash preparation is an alternative to replace mouthwash preparations which generally contain an antiseptic in the form of alcohol which can trigger oral cancer. This research is to formulate gambir as a mouthwash and determine the differences in the effectiveness of antibacterial on the growth of Staphylococcus aureus with variations in the concentration of 5%, 10%, 15%. Antibacterial activity test using NA media with agar diffusion method. Physical requirements tests include organoleptic tests, pH tests and viscosity testsshowed that the preparation of 5%, 10% and 15% gambir mouthwash had a pH outside the optimum bacterial growth range of 5. Viscosity of mouthwash preparations of 1.30-5.10 Cp. The antibacterial activity of Staphylococcus aureus in F1 did not produce inhibitory zones, F2 was 5.46 mm, F3 was 9.60 mm and F4 was 10.76 mm while in f5 the positive control was 12.10 mm. Conclusio of this study is gambier mouthwash concentration of 5%, 10% and 15% can inhibit the growth of staphylococcus aureus bacteria.

scholarly journals In vitro Activity of Rambutan Binjai (Nephelium lappaceum) Peel Extract from Indonesia to Methicillin-Resistant Staphylococcus aureus (MRSA)

2018 ◽  
Vol 17 (2) ◽  
pp. 197-203
Author(s):  
Tina Rostinawati ◽  
Ami Tjitraresmi ◽  
Myra Vania Wisnuputri
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Methicillin Resistant ◽  
Nephelium Lappaceum ◽  
New Compounds ◽  
Peel Extract

Methicillin-resistant Staphylococcus aureus (MRSA) is the most common bacteria causing nosocomial infections with high levels of resistance to available antibiotics. So, it is necessary to search for new compounds to solve this problem. Various studies showed antibacterial activity of rambutan peel but for Rambutan Binjai peel extract that are from Indonesia has never been studied against the MRSA. This study aims to determine the antibacterial activity, the value of minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) using agar diffusion method. The concentration of rambutan peel ethanol extract at as much as 62.5 mg/ml showed the inhibitory diameter i.e 21.3 ± 2.4 mm. MIC and MBC were in the same range, which was between 0.98 (mg/ml) to 1.95 (mg/ml). The activity strength of tetracycline against the extract was at 1:50. This revealed that Rambutan Binjai peel extract had great potency as antibacterial agent to MRSA. Dhaka Univ. J. Pharm. Sci. 17(2): 197-203, 2018 (December)

Antibacterial Activity of Nanoparticles Produced by Ethanol Extract of Boesenbergia rotunda Rhizome Loaded with Chitosan and Alginic Acid Against Streptococcus mutans by In Vitro

2017 ◽  
Vol 7 (03) ◽  
Author(s):  
Sri Atun ◽  
Sri Handayani ◽  
Melia Aliffiana ◽  
Hajar Nur Afifah ◽  
Anna Rakhmawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
Alginic Acid ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

scholarly journals IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF 1.5-BIS (3’-ETHOXY-4’-HYDROXYPHENYL)-1-4-PENTADIENE-3-ONE

2018 ◽  
Vol 10 (5) ◽  
pp. 70
Author(s):  
Agus Purwanggana ◽  
Esti Mumpuni ◽  
Esti Mulatsari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Staphylococcus Epidermidis ◽  
In Silico ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Antibacterial Study

Objective: The main objective of this research were screened in vitro and in silico of 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one as potential antibacterial agents.Methods: The in vitro antibacterial study was carried against Staphylococcus aureus, Staphylococcus epidermidis (gram positive) and Escherichia coli, Salmonella thypi (gram negative) using broth dilution method to determine Minimum Inhibitory Concentration (MIC), disc diffusion method to determine the diameter of inhibition zone. In silico antibacterial study was carried using computational software Protein-Ligand ANT System (PLANTS), computational docking was carried using receptor with Protein Data Bank (PDB) file 3MZD. The structures were optimized prior docking using YASARA, and MarvinSketch. The results of antibacterial testing were compared to two positive control drugs i. e amoxicillin and cefadroxil.Results: In vitro evaluation showed that 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has a better antibacterial activity than amoxicillin and cefadroxil with a Minimum Inhibitory Concentration (MIC) of 0.15 ppm and diameter of inhibition zone of 11.27±0.31, 11.35±0.39, 11.25±0.33, and 11.05±0.45 mm in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Salmonella thypi, respectively. These results in line with in silico evaluation that showed 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has more negative docking score than amoxicillin, cefadroxil, and cloxacillin acyl as a native ligand on the 3MZD receptor.Conclusion: This results obtained in this research work were 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one compound potential as an antibacterial agent. 

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