The effect of indole-butyric acid and kinetin on rooting of rose cuttings in winter and summer

The effect of indole-butyric acid, both alone and with a low concentration of kinetin, on the rooting of rose cuttings in winter and summer, is presented in this paper. The experiments were conducted using 500 and 1000 mg/l IBA with or without the addition of 5 mg/l kinetin. The growth regulators were applied by dipping the base of a cutting for 5 s in an aqueous solution of these substances. Cuttings 5-6 cm in length were made from the mid-part of a stem of a rose grown in a greenhouse. The experiments were carried out using 'Queen of Bermuda' and 'Baccara' cuttings. The investigations showed that treating rose cuttings rooted in winter with an IBA solution had a significant promotive effect on the quantity of rooted cuttings, number of formed roots on the cutting, as well as on the length of the longest root. A distinctive increase in the number of breaking buds was also seen on the cuttings treated with IBA. The IBA solution applied to cuttings rooted in the summer significantly decreased the number of rooted cuttings and breaking buds. However, no significant influence on the number and length of formed roots was found. Addition of kinetin to the IBA solutions did not have any effect on the rooting of rose cuttings either in winter or summer.

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Rooted cuttings by IBA (Indole butyric acid) and NAA (Naphthalene acetic acid) in black pepper (piper nigrum) - A case study in Vietnam

International Journal of Chemical Studies ◽

10.22271/chemi.2020.v8.i3z.9480 ◽

2020 ◽

Vol 8 (3) ◽

pp. 1880-1885

Author(s):

Tran Quyen Nguyen ◽

Ba Huy Nguyen ◽

Dieu Hien Tran Thi ◽

Oanh Duong Thi ◽

Quang Ngoc Nguyen ◽

...

Keyword(s):

Acetic Acid ◽

Butyric Acid ◽

Black Pepper ◽

Piper Nigrum ◽

Naphthalene Acetic Acid ◽

Indole Butyric Acid ◽

Rooted Cuttings

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PROPAGATING ROSEMARY (ROSEMARINUS OFFICINALIS) BY CUTTINGS

HortScience ◽

10.21273/hortsci.26.5.487e ◽

1991 ◽

Vol 26 (5) ◽

pp. 487e-487

Author(s):

Nancy E. Maness ◽

James E. Motes

Keyword(s):

Butyric Acid ◽

Indole Butyric Acid ◽

Rooted Cuttings ◽

Rooted Cutting ◽

The One ◽

Methods Of Application

`Arp' rosemary cuttings were treated with indole butyric acid (IBA) using three different application procedures to determine speed and quality of rooted cuttings produced at three weeks under mist. Five concentrations of IBA, 0%, 0.8%, 1.6%, 3.0%, and 4.5%, and three methods of application were compared. Methods of application were 1) cuttings treated with solutions containing IBA, captan, benomyl, and streptomycin applied as a one minute soak or 2) as a post plant drench or 3) cuttings soaked for one minute in a solution of captan, benomyl and streptomycin and then dipped into IBA + talc mixture. After the treatments were applied, the cuttings were placed on a mist bench for three weeks. Visual shoot ratings were made weekly and visual root ratings were made at three and four weeks after treatments were applied. Root dry weights were determined. Results indicated the one minute soak in a captan, benomyl, and streptomycin solution then dipped in 0.8% IBA + talc consistently resulted in a higher quality rooted cutting.

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Stability of 4,4,4-Trifluoro-3-(indole-3-)butyric Acid Dissolved in Water on Growth of Rice Roots

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1460 ◽

2012 ◽

Vol 550-553 ◽

pp. 1460-1463

Author(s):

Xiu Ping Li ◽

Yan Lai Zhang ◽

Sasakawa Hideo

Keyword(s):

Aqueous Solution ◽

Butyric Acid ◽

Root Elongation ◽

Room Temperature ◽

Microwave Oven ◽

Promotion Effect ◽

Direct Heating ◽

Rice Roots ◽

Promotive Effect ◽

The Stability

The stability of 4,4,4-trifluoro-3-(indole-3-) butyric acid (TFIBA) were examined. Elongation of rice seminal root was promoted approximately 80% by continuous supply of 10-5 molL-1 TFIBA. Such promotion effect on root elongation is not found in IAA or IBA. The promotive effect of TFIBA on root elongation was not reduced by either direct heating in a microwave oven or autoclaving at 121°C for 15 min and root elongation was promoted by 70 - 80% at the concentration of 10-5 mol L-1. The effect of TFIBA was not lost by heat of microwave oven or autoclave. Although the effect of TFIBA dissolved in water was gradually lost at 30°C in the light, it was maintained at 4°C in the light and at 30°C in the dark. Consequently, aqueous solution of TFIBA can be stored at room temperature for at least 1 month when it is kept in the dark.

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Characteristics of Litchi Seed Germination

HortScience ◽

10.21273/hortsci.31.7.1187 ◽

1996 ◽

Vol 31 (7) ◽

pp. 1187-1189 ◽

Cited By ~ 6

Author(s):

J.S. Prasad ◽

Raj Kumar ◽

Mukund Mishra ◽

Rajesh Kumar ◽

A.K. Singh ◽

...

Keyword(s):

Seed Germination ◽

Relative Humidity ◽

Gibberellic Acid ◽

Butyric Acid ◽

Growth Regulators ◽

Filter Paper ◽

Seed Viability ◽

Indole Butyric Acid ◽

Polyethylene Bags ◽

Better Than

Seed germination of four Litchi chinensis Sonn. cultivars (`Deshi', `Kasba', `Purbi', and `Early Bedana') was studied under various conditions, viz. in soil beds exposed to sunlight or in shade, in sand beds exposed to sunlight or in shade, and on moist filter paper. Among all, shaded, humid sand at 35 ± 2C gave the highest germination. Delaying sowing seeds after removal from the fruit significantly reduced germination. Litchi seeds held in polyethylene bags up to 4 days at 37 ± 2C at 90% relative humidity delayed loss of seed viability. Germination was improved by ethephon in `Deshi' and `Early Bedana', by IBA in `Deshi' and `Purbi', and by 100 mm GA3 in all litchi cultivars. Cultivars responded differently to growth regulators, with `Deshi' responding significantly better than `Purbi', `Kasba', or `Early Bedana'. These studies point to the recalcitrant nature of litchi seeds. Chemical names used: gibberellic acid (GA3); indole butyric acid (IBA); 2-chloroethylphosphonic acid (ethephon).

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Role of Auxin and Microclimate on the Success of Rooted Cuttings of Cocoa

Pelita Perkebunan (a Coffee and Cocoa Research Journal) ◽

10.22302/iccri.jur.pelitaperkebunan.v23i1.52 ◽

2007 ◽

Vol 23 (1) ◽

Author(s):

Adi Prawoto ◽

Arifin . ◽

Syamsul Bachri ◽

K.C Setyaningtyas

Keyword(s):

Light Intensity ◽

Butyric Acid ◽

Root Number ◽

Indole Butyric Acid ◽

Rooted Cuttings ◽

Randomized Design ◽

Auxin Content ◽

Main Plot ◽

Genetic And Environmental Factors ◽

Available Technology

In Indonesia, cocoa reproduction by cuttings is undeveloped yet because the available technology is more expensive than the other clonal reproduction methods. The success of cocoa cuttings is influenced by genetic and environmental factors. The purpose of this research is to study effect of endogenous auxin content, effects of light intensity and exogenous auxin application, on the rooted cuttings. The 2 nd research purpose is to study effects of PVP (Polyvinylpyrrolidon) and IBA (β-indole-butyric acid), clones, and microclimate. The experiment was conducted in Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute in Jember (45 m a.s.l. and D rainfall type according to Schmidt Ferguson). The design for the 1 st experiment was splitsplit plot, replicated three times. The main plot was light intensity inside the roof, i.e.15%, 30%, and 45% to direct sun radiation. The sub plot was cocoa clones, i.e. DR 2 and ICS 13, and the sub-sub plot was IBA concentration, i.e. 0 ppm, 1500 ppm, 3000 ppm and 4500 ppm. The 2 nd experiment was designed factorial 3 x 3 of CRD, replicated 3 times. Clones of KW 163, KW 162 and KW 165, and IBA at 0, 3000, and 6000 ppm were the factors. In the same time, effect of IBA 6000 ppm, PVP 6000 ppm IBA+PVP 6000 ppm, and control were observed using KW 165 clone, and designed in complete randomized design (CRD), replicated 3 times. The result showed that auxin content of ICS 13 was higher than DR 2 (62.67 ppm vs 40.90 ppm) so that gave higher rooted cuttings and more root number. Exogenous application of IBA improved auxin content of the cutting materials and promoted root growth. The optimum IBA concentration for root number was 3500 ppm. Light intensity of 45% improved number of rooted cuttings three times compared to 15%, however compared to the 2 nd research, percentage of rooted cuttings was still very low. Cocoa cutting method to gain rooted cuttings 80—90% has been obtained. The method was using IBA 3000 ppm or 6000 ppm mixtured with or without PVP 6000 ppm, conducted during rainy season, the nursery using permanent shade trees of leucena sp., temperature of 24—27oC and relative humidity was 78—87%. PVP was supposed inhibit oxidation of IBA so that the effect of IBA was more optimum. The response of KW 162, KW 163 and KW 165 clones to root were similar. Rooted cuttings during dry season was low due to the less fresh of cutting materials and high temperature inside the bed roof. Key Words: Theobroma cacao, cuttings, light intensity, auxin, β-indole-butyric acid, Polyvinylpyrrolidon

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Pengaruh Hormon Indole Butyric Acid (IBA) dan 6-Benzyl Amino Purin (BAP) terhadap Induksi Kalus Piper betle L. var Nigra

Journal of Pharmacy and Science ◽

10.53342/pharmasci.v4i2.141 ◽

2019 ◽

Vol 4 (2) ◽

pp. 85-90

Author(s):

Junairiah . ◽

Artifa Rachmah ◽

Yosephine Sri Wulan Manuhara ◽

Ni’matuzahroh . ◽

Lilis Sulistyorini ◽

...

Keyword(s):

Butyric Acid ◽

Growth Regulators ◽

Callus Induction ◽

Callus Formation ◽

Dry Weight ◽

Piper Betle ◽

Fresh Weight ◽

Indole Butyric Acid ◽

Randomized Design ◽

Benzyl Amino Purine

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh kombinasi konsentrasi zat pengatur tumbuh IBA (Indole Butyric Acid) dan BAP (6-Benzyl Amino Purine) yang paling baik untuk induksi kalus sirih hitam (Piper betle L.). Penelitian ini menggunakan rancangan acak lengkap dengan 25 perlakuan dan setiap perlakuan memiliki 6 ulangan sehingga terdapat 150 unit ekperimen. Pada tahap kultur kalus dilakukan dengan menambahkan zat pengatur tumbuh IBA dan BAP ke dalam medium Murashige and Skoog (MS). Hasil uji tersebut menunjukkan bahwa zat pengatur tumbuh IBA dan BAP dengan kombinasi konsentrasi berbeda berpengaruh terhadap waktu induksi kalus, berat segar dan berat kering kalus sirih hitam. Hasil penelitian menunjukkan waktu tercepat pembentukan kalus pada IBA 2,0 mg/L dan BAP 2,0 mg/L yaitu 10 hari. Berat segar dan berat kering tertinggi pada IBA 2,0 mg/L dan 2,0 mg/L yaitu 0,8507 gram untuk berat segar dan 0,0769 untuk berat kering. Warna kalus adalah putih kehijauan dengan tekstur kompak dan remah.Kata kunci: Induksi kalus, Piper betle L., Indole Butyric Acid, 6-Benzyl Amino Purine.ABSTRACTThe purpose of this research to determine the effect of the combination concentration of growth regulators IBA (Indole Butyric Acid) and BAP (6-Benzyl Amino Purine) was best for callus induction black betel (Piper betle L.). This research used completely randomized design with 25 treatments and 6 replicates of each treatment, hence there were 150 experimental units. At this stage of callus culture was done by adding the growth regulators IBA and BAP into Murashige and Skoog (MS). The test results showed that plant growth regulators IBA and BAP in combination with different concentrations of influence on callus induction time, fresh weight and dry weight callus Piper betle L. The results showed the fastest time of callus formation at IBA 2,0 mg/L and BAP 2,0 mg/L at 10 days. Fresh weight and dry weight of the highest in the IBA 2,0 mg/L and BAP 2,0 mg/L were 0,8507 grams and 0,0769 grams fresh weight to dry weight. The color of callus was white greenish with compact and friable texture.Keywords: Callus induction, Piper betle L., Indole Butyric Acid, 6-Benzyl Amino Purine.

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Effect of Cow Urine and IBA on the Growth of Sweet Leaf (Sauropus androgynus (L.) Merr.) Cuttings

JURNAL AGRONIDA ◽

10.30997/jag.v5i1.1854 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Fawziyah Nurshabrina ◽

Arifah Rahayu ◽

Oktavianus Lt

Keyword(s):

Growth Regulators ◽

Plant Roots ◽

Indole Butyric Acid ◽

Rooted Cuttings ◽

Shoot Height ◽

Cow Urine ◽

Two Factors ◽

Number Of Leaves ◽

Leaf Vegetable ◽

Number Of Shoots

Sweet leaf (Sauropus androgynus (L.) Merr.), a leaf vegetable that is generally propagated vegetatively with cuttings. This study aims to determine the effect of accession and administration of natural and synthetic auxin growth regulators on the growth of sweet leaf cuttings. This study uses factorial Randomized Complete Design consisting of two factors, namely accession (Banten from Pandeglang, Bogor from Tajur, and Cianjur from Cugenang) and auksin combination (IBA 100%R, urine 25%R + IBA 75%R, urine 50%R + IBA 50%R, urine 75%R + IBA 25%R, and urine 100%R). The results showed that the growth of Cianjur accession had the best results on the percentage of growth cuttings, the percentage of shoot buds, shoot height, number of shoots, number of leaves, percentage of rooted cuttings, and number of roots compared to Banten and Bogor accessions. Giving urine 100%R significantly increases plant height, shoot height, number of shoots, and number of sweet leaf plant roots compared with other auxin combinations. The combination treatment of auxin and accession showed results that were not significantly different from the variable root length and weight of the harvest. In sweet leaf plants, cow urine can replace the use of IBA (Indole Butyric Acid). Keywords: Sauropus androgynus, vegetative, urine, IBA

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Effects of Wounding, IBA and Basal Trimming on Rooting of Boxwood Cuttings

Journal of Environmental Horticulture ◽

10.24266/0738-2898-4.3.72 ◽

1986 ◽

Vol 4 (3) ◽

pp. 72-73

Author(s):

Thomas J. Banko ◽

Marcia A. Stefani

Keyword(s):

Aqueous Solution ◽

Butyric Acid ◽

Potassium Salt ◽

Indole Butyric Acid ◽

Basal Node ◽

Buxus Sempervirens

Wounding, indole butyric acid (IBA) and trimming of cuttings to a basal node were evaluated for their effects on rooting of Buxus sempervirens L. (American boxwood), B. sempervirens L. ‘Suffruticosa’ (English boxwood), B. microphylla var. koreana Nakai. (Korean boxwood), and B. microphylla var. japonica (Müll. Agr.) Rehd. and Wils. (Japanese boxwood) cuttings. Rooting of all cultivars was improved by a 5 second dip in a 0.4% aqueous solution of the potassium salt of IBA. Wounding the basal end of the cuttings only improved rooting for Korean and Japanese boxwoods. However, the combination of wounding and IBA dip gave the best results for all cultivars. Trimming the cuttings to a basal node was beneficial only to Korean boxwoods.

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Lipid and Yield Evaluation in Salicornia bigelovii by the Influence of Chitosan-IBA, in Conditions of the Sonora Desert

Agronomy ◽

10.3390/agronomy11030428 ◽

2021 ◽

Vol 11 (3) ◽

pp. 428

Author(s):

Gerardo Zapata-Sifuentes ◽

Pablo Preciado-Rangel ◽

Reyna Roxana Guillén-Enríquez ◽

Francisca Sánchez Bernal ◽

Ramon Jaime Holguin-Peña ◽

...

Keyword(s):

Lipid Profile ◽

Seed Production ◽

Butyric Acid ◽

Lipid Content ◽

Production System ◽

Large Scale ◽

Physicochemical Analysis ◽

Indole Butyric Acid ◽

Salicornia Bigelovii ◽

Yield Production

The present investigation aimed to evaluate the effect of Chitosan-Indole Butyric Acid (IBA) in the seed of Salicornia bigelovii under field conditions in Sonora, Mexico. During two vegetative cycles (2018/2019–2019/2020), cuttings of S. bigelovii were treated with 100 and 50% Chitosan from shrimp exoskeletons and indole butyric acid at 0.937 and 1.25 g·kg−1 and placed in basins under conditions of the Sonora desert, Mexico. Variables were measured: seed production, physicochemical analysis and lipid profile of the seed. The results affected significant increases (p < 0.05) in the evaluated variables, highlighting the treatment based on Chitosan 100%—IBA 0.937 g·kg−1. The results based on chitosan and IBA in cuttings, are a biostimulant in the morpho-physiology, yield production, and lipid content of S. bigelovii. Large-scale studies as a production system should be considered in further studies.

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Submist is Effective for Propagation of Korean Lilac and Inkberry by Stem Cuttings

HortTechnology ◽

10.21273/horttech03970-18 ◽

2018 ◽

Vol 28 (3) ◽

pp. 378-381

Author(s):

Bryan J. Peterson ◽

Stephanie E. Burnett ◽

Olivia Sanchez

Keyword(s):

Butyric Acid ◽

Woody Plants ◽

Root Biomass ◽

Potassium Salt ◽

Foliar Nutrients ◽

Stem Cuttings ◽

Rooted Cuttings ◽

Soilless Substrate

Although overhead mist revolutionized the propagation industry, it does suffer from potential drawbacks that include the application of large volumes of water, potentially unsanitary conditions, irregular misting coverage, and leaching of foliar nutrients. We explored the feasibility of submist as an alternative as it might avoid these problems by applying water exclusively from below the cutting, which is inserted basally into an enclosed rooting chamber. We propagated cuttings of korean lilac (Syringa pubescens ssp. patula) and inkberry (Ilex glabra) using both overhead mist and submist to compare effectiveness of the systems. Cuttings of korean lilac were wounded and dipped basally into 8000 mg·L−1 of the potassium salt of indole-3-butyric acid (K-IBA), and those in the overhead mist systems were inserted into coarse perlite. Cuttings of inkberry were wounded and treated with 5000 mg·L−1 K-IBA, and those in the overhead mist systems were inserted into 50:50 peat:perlite (by vol). Cuttings of korean lilac in the submist systems produced more than twice as many roots as cuttings in the overhead mist systems, with roots more than 2.6 times the length. Similarly, cuttings of inkberry in the submist systems produced more than three times the root counts and root lengths as cuttings in the overhead mist systems. For korean lilac, root dry weights averaged 58 mg for cuttings in the submist system, compared with only 18 mg among cuttings receiving overhead mist. Likewise, root dry weights averaged 70 and 7 mg for cuttings of inkberry propagated by submist and overhead mist, respectively. Rooted cuttings of korean lilac transplanted well into a soilless substrate, where they more than tripled their root biomass to 218 mg (vs. 59 mg for cuttings transplanted from overhead mist). We did not evaluate transplant performance of inkberry. Our results show that submist systems might merit consideration for the propagation of woody plants by leafy stem cuttings.

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