scholarly journals Role of Auxin and Microclimate on the Success of Rooted Cuttings of Cocoa

2007 ◽  
Vol 23 (1) ◽  
Author(s):  
Adi Prawoto ◽  
Arifin . ◽  
Syamsul Bachri ◽  
K.C Setyaningtyas
Keyword(s):  
Light Intensity ◽  
Butyric Acid ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Rooted Cuttings ◽  
Randomized Design ◽  
Auxin Content ◽  
Main Plot ◽  
Genetic And Environmental Factors ◽  
Available Technology

In Indonesia, cocoa reproduction by cuttings is undeveloped yet because the available technology is more expensive than the other clonal reproduction methods. The success of cocoa cuttings is influenced by genetic and environmental factors. The purpose of this research is to study effect of endogenous auxin content, effects of light intensity and exogenous auxin application, on the rooted cuttings. The 2 nd research purpose is to study effects of PVP (Polyvinylpyrrolidon) and IBA (β-indole-butyric acid), clones, and microclimate. The experiment was conducted in Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute in Jember (45 m a.s.l. and D rainfall type according to Schmidt Ferguson). The design for the 1 st experiment was splitsplit plot, replicated three times. The main plot was light intensity inside the roof, i.e.15%, 30%, and 45% to direct sun radiation. The sub plot was cocoa clones, i.e. DR 2 and ICS 13, and the sub-sub plot was IBA concentration, i.e. 0 ppm, 1500 ppm, 3000 ppm and 4500 ppm. The 2 nd experiment was designed factorial 3 x 3 of CRD, replicated 3 times. Clones of KW 163, KW 162 and KW 165, and IBA at 0, 3000, and 6000 ppm were the factors. In the same time, effect of IBA 6000 ppm, PVP 6000 ppm IBA+PVP 6000 ppm, and control were observed using KW 165 clone, and designed in complete randomized design (CRD), replicated 3 times. The result showed that auxin content of ICS 13 was higher than DR 2 (62.67 ppm vs 40.90 ppm) so that gave higher rooted cuttings and more root number. Exogenous application of IBA improved auxin content of the cutting materials and promoted root growth. The optimum IBA concentration for root number was 3500 ppm. Light intensity of 45% improved number of rooted cuttings three times compared to 15%, however compared to the 2 nd research, percentage of rooted cuttings was still very low. Cocoa cutting method to gain rooted cuttings 80—90% has been obtained. The method was using IBA 3000 ppm or 6000 ppm mixtured with or without PVP 6000 ppm, conducted during rainy season, the nursery using permanent shade trees of leucena sp., temperature of 24—27oC and relative humidity was 78—87%. PVP was supposed inhibit oxidation of IBA so that the effect of IBA was more optimum. The response of KW 162, KW 163 and KW 165 clones to root were similar. Rooted cuttings during dry season was low due to the less fresh of cutting materials and high temperature inside the bed roof. Key Words: Theobroma cacao, cuttings, light intensity, auxin, β-indole-butyric acid, Polyvinylpyrrolidon

scholarly journals Rooted cuttings by IBA (Indole butyric acid) and NAA (Naphthalene acetic acid) in black pepper (piper nigrum) - A case study in Vietnam

2020 ◽  
Vol 8 (3) ◽  
pp. 1880-1885
Author(s):  
Tran Quyen Nguyen ◽  
Ba Huy Nguyen ◽  
Dieu Hien Tran Thi ◽  
Oanh Duong Thi ◽  
Quang Ngoc Nguyen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Rooted Cuttings

scholarly journals Response of ‘Bignay’ [Antidesma bunius (Linn.) Spreng] to cutting origins, IBA and BioGroe treatments

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Edgar V Benabise ◽  
Jonalyn J Quinan ◽  
Joel G Carig
Keyword(s):  
Butyric Acid ◽  
Interaction Effect ◽  
Shoot Length ◽  
Root Number ◽  
Percent Survival ◽  
Randomized Design ◽  
Study Results ◽  
Completely Randomized Design ◽  
Different Levels ◽  
Bottom Portion

The response of ‘Bignay’ [Antidesma bunius (Linn.) Spreng] to the cutting origins and different levels of plant bio-regulators consist of Indole-3-butyric Acid (IBA) and Biogroe treatments were investigated by means of 3 x 9 factorial experiment in Completely Randomized Design (CRD) using an automated mist propagator. Two hundred sixteen (216) healthy seedlings containing 9 nodes each were used in the study. Results revealed that cutting origins significantly increased shoot length but have no influence on the root number, percent rooting and percent survival. The cuttings originated from the bottom portion of the stem recorded the longest mean in terms of shoot length (12.48 mm) including the highest percent survival and percent rooting (82.41%). Highest mean number of roots were observed on the top cuttings (1.93). Indole-3-butyric Acid (IBA) and Biogroe treatments on cuttings have no effects on the different parameters evaluated. The interaction effect between cutting origins and IBA/Biogroe treatments significantly increased the percent rooting and percent survival except the shoot length and root number of Bignay cuttings. Overall, the findings inferred that A. bunius can be propagated by any cutting origin derived from the main stem of the donor plants tested. Cuttings can effectively be induced to produce roots and survive and can be economically mass propagated even without the application of different concentrations of IBA and BioGroe.

scholarly journals PROPAGATING ROSEMARY (ROSEMARINUS OFFICINALIS) BY CUTTINGS

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 487e-487
Author(s):  
Nancy E. Maness ◽  
James E. Motes
Keyword(s):  
Butyric Acid ◽  
Indole Butyric Acid ◽  
Rooted Cuttings ◽  
Rooted Cutting ◽  
The One ◽  
Methods Of Application

`Arp' rosemary cuttings were treated with indole butyric acid (IBA) using three different application procedures to determine speed and quality of rooted cuttings produced at three weeks under mist. Five concentrations of IBA, 0%, 0.8%, 1.6%, 3.0%, and 4.5%, and three methods of application were compared. Methods of application were 1) cuttings treated with solutions containing IBA, captan, benomyl, and streptomycin applied as a one minute soak or 2) as a post plant drench or 3) cuttings soaked for one minute in a solution of captan, benomyl and streptomycin and then dipped into IBA + talc mixture. After the treatments were applied, the cuttings were placed on a mist bench for three weeks. Visual shoot ratings were made weekly and visual root ratings were made at three and four weeks after treatments were applied. Root dry weights were determined. Results indicated the one minute soak in a captan, benomyl, and streptomycin solution then dipped in 0.8% IBA + talc consistently resulted in a higher quality rooted cutting.

scholarly journals PERTUMBUHAN SETEK KRISAN (Chrysanthemum sp.) PADA BERBAGAI KONSENTRASI HORMON IBA (Indole Butyric Acid) DI BANGKA DENGAN SISTEM EX-VITRO

2020 ◽  
Vol 3 (1) ◽  
pp. 589-602
Author(s):  
Anggri Restikadia ◽  
Keyword(s):  
Experimental Method ◽  
Butyric Acid ◽  
Research Method ◽  
Ex Vitro ◽  
Indole Butyric Acid ◽  
Randomized Design ◽  
Flower Plant ◽  
Minimum Potential ◽  
Completely Randomized Design ◽  
Experimental Garden

Chrysanthemum is an ornamental flower plant that has advantage because of various colors and has long vase time. The minimum potential for chrysanthemum development in Bangka is because of limited seed and the enviroment conditions in Bangka are less than optimal for chrysanthemum plants. The increasing chrysanthemum seedlings can be done vegetatively by addition of IBA hormone with an ex-vitro system. This study aims to determine the effect of IBA (Indole Butyric Acid) hormone on the growth of chrysanthemum cuttings in Bangka. This research was conducted from March to May 2020 at the Research and experimental Garden, Faculty of Agriculture, Fisheries and Biology, Universitas Bangka Belitung. The research method used an experimental method with a single completely randomized design (CRD) consisting of 6 treatments, namely (Control), IBA 50 ppm, IBA 100 ppm, IBA 150 ppm, IBA 200 ppm, IBA 250 ppm. The results showed that giving IBA had no significant effect on the growth of chrysanthemum cuttings in Bangka. Treatment (IBA 150 ppm) tends to be better for the growth of chrysanthemum cuttings in Bangka.

scholarly journals Clonal Propagation of Coffea arabica with Indole Butyric Acid and Acclimatization Conditions in Amazonas, Peru

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Jardy Chichipe Oyarce ◽  
Alina Camacho ◽  
Leidy G. Bobadilla ◽  
Carmen N. Vigo ◽  
Héctor V. Vásquez ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Clonal Propagation ◽  
Leaf Length ◽  
Indole Butyric Acid ◽  
Randomized Design ◽  
Rooting Of Cuttings ◽  
Completely Randomized Design ◽  
Stage 1 ◽  
Two Stages ◽  
Typical Variety

The use of hormones is important in improving the propagation process. The objective of the experiment was to identify the best dose of indole butyric acid (IBA) hormone for the rooting of coffee cuttings of the typical variety through clonal propagation in the Amazon region. The experiment was carried out in two stages. Both stages were carried out under a Completely Randomized Design (CRD). The percentage of rooting of cuttings and the number of roots were evaluated. The coffee tree clones were collected from rust tolerant elite plants. In the laboratory, the cuttings were immersed for 10 minutes in fungicide and then in five doses of IBA hormone (1000, 1500, 2000, 2500, and 3000 mg L−1) of IBA. They were then placed in a microtunnel. With the best dose obtained in stage 1 of IBA (T4: 2000 mg L−1), which achieved a rooting percentage of 52.50% and 5.8 roots on average, stage 2 was installed for the evaluation of acclimatization conditions (greenhouse and nursery). Thirty plants of the Típica variety were evaluated per treatment. Regarding the results after 90 days, the greenhouse obtained the highest values with 72.22% survival, a plant height of 9.48 cm, 4.32 mm stem diameter, 6.53 leaves per plant, 9.65 cm leaf length, and 3.83 cm leaf width. The IBA hormone in a controlled environment such as the greenhouse achieves good vegetative propagation for the Típica variety.

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

scholarly journals Rooting Ability of Hardwood Cuttings in Highbush Blueberry (Vaccinium corymbosum L.) Using Different Indole-butyric Acid Concentrations

HortScience ◽  
2018 ◽  
Vol 54 (2) ◽  
pp. 194-199 ◽  
Author(s):  
Haishan An ◽  
Jiajia Meng ◽  
Fangjie Xu ◽  
Shuang Jiang ◽  
Xiaoqing Wang ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Root Length ◽  
Vaccinium Corymbosum ◽  
Control Treatment ◽  
Highbush Blueberry ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Rooting Ability ◽  
Rooting Response ◽  
Main Factor

Vegetative propagation by cuttings is a very popular method. However, blueberry propagation using cuttings is still a main factor limiting its expansion because its results can vary according to the blueberry cultivar and environmental factors. This study aimed to evaluate the rooting abilities of hardwood cuttings for six blueberry cultivars (O’Neal, Misty, Diana, Biloxi, Bluebeauty, and Coville) using three different exogenous indole-butyric acid (IBA) concentrations (1000, 2000, and 3000 ppm), and to determine if the cutting position (basal, central, apical) affects rooting performance. A control treatment (0 ppm IBA) was also performed. After 90 days of each treatment, rooting percentage, average root length, and average root number per cutting were assessed and used to calculate rooting index, which is a measure of rooting ability. The rooting percentages of hardwood cuttings differed largely among cultivars and were highest for ‘Bluebeauty’ (68.55%), followed by ‘Biloxi’ (68.01%). The rooting index values of these two cultivars (33.59 and 35.18, respectively) were significantly higher than those of the other four cultivars. The rooting response of blueberry hardwood cuttings to IBA concentrations was quadratic, and 1000 and 2000 ppm IBA were sufficient to express the maximum rooting percentage in most cultivars. The rooting abilities of basal, central, and apical cuttings were similar with treatments with high IBA concentrations. The effects of the cultivar, IBA concentration, and interaction between them on rooting percentage, average root length, and average root number were significant; however, the effects of the cutting position on the rooting percentage and average root length were not. This suggested that the rooting abilities of blueberry hardwood cuttings were significantly influenced by the cultivar and IBA concentration rather than by the cutting position.

scholarly journals Effect of Indole-3-Butyric Acid on Clonal Propagation of Swietenia macrophylla through Branch Cutting

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Md. Salim Azad ◽  
Md. Abdul Matin
Keyword(s):  
Overall Survival ◽  
Butyric Acid ◽  
Clonal Propagation ◽  
Root Number ◽  
Swietenia Macrophylla ◽  
Rooted Cuttings ◽  
Survival Percentage ◽  
Shoot Number ◽  
Number Of Leaves ◽  
Branch Cutting

The study discloses the scopes of clonal propagation Swietenia macrophylla through branch cutting treated with IBA. A total of four hundred cuttings were used with four replications to assess the rooting ability. The study exposed significant (p<0.5) difference of rooted cuttings among the treatments. The highest (62.51%) rooting percent was observed in cutting with 0.4% IBA treatment. In addition, root number and its length per cutting were increased with increasing IBA concentration (p<0.5). The experiment showed significant (p<0.5) difference of sprouting among the treatments. The highest (67.27%) percent of sprouting was observed in cuttings with 0.4% solution, which showed a similar fashion of percent of rooting. The study also showed significant (p<0.5) difference of shoot number per cutting, the length of the longest shoot, and number of leaves per cutting among the treatments. The overall survival of rooted cuttings after transfer to polybag significantly (p<0.5) differed among the cuttings treated with different IBA solution. The highest survival percentage (69.67%) was observed in the cuttings rooted with 0.4% IBA treatment and the lowest (55.6%) survival was found in cuttings treated with control. The use of 0.4% IBA treatment is suggested for rooting of juvenile leafy branch cutting of S. macrophylla.

scholarly journals Pertumbuhan Tanaman Nanas pada Berbagai Konsentrasi IBA Secara In Vitro

2014 ◽  
Vol 4 (2) ◽  
pp. 287-292
Author(s):  
Zarmiyeni Zarmiyeni ◽  
Siti Munawarah Munawarah
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Butyric Acid ◽  
Market Demand ◽  
Indole Butyric Acid ◽  
The People ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
A New Technique

       The people of Hulu Sungai Utara Regency are very fond of pineapple, this can be seen from the continued increase in market demand for pineapple. Pineapple production in Hulu Sungai Utara Regency from 2010-2013 continues to increase. However, the availability of pineapple is still widely supplied from other regions. This is due to limited land and the availability of superior seeds. Therefore we need a new technique to overcome this problem by multiplying it in vitro. This study aims (i) to determine the response of pineapple plant growth to various concentrations of IBA (Indole Butyric Acid) in vitro and (ii) to obtain the best IBA (Indole Butyric Acid) concentration for pineapple plant growth in vitro. The study was conducted in the Amuntai STIPER tissue culture laboratory from March to June 2014, using a Completely Randomized Design (CRD) with 5 treatment levels, 4 replications. The factor tested was the IBA concentration, i1 = 1.5 ppm, i2 = 2 ppm, i3 = 2.5 ppm, i4 = 3 ppm, and i5 = 3.5 ppm. The results showed no known response and the best concentration of IBA on pineapple plant growth.

scholarly journals Respon Setek Cabang Bambu Betung (Dendrocalamus Asper) Akibat Pemberian Asam Indol Butirat (AIB)

2016 ◽  
Vol 4 (2) ◽  
pp. 61
Author(s):  
Erviana Sari ◽  
Indriyanto . ◽  
Afif Bintoro
Keyword(s):  
Butyric Acid ◽  
Experimental Unit ◽  
Development Research ◽  
Indole Butyric Acid ◽  
Randomized Design ◽  
Shoot Number ◽  
Bamboo Plantation ◽  
Dendrocalamus Asper ◽  
Completely Randomized Design ◽  
Branch Cutting

Betung bamboo (Dendrocalamus asper) as multipurpose plant could be developed with vegetative and generative propagation.  Branch cuttings was the easiest way in the betung bamboo vegetative propagation.  One of plant growth regulators that could be used to spur betung bamboo branch cutting was indole butyric acid (IBA).  This study was conducted to evaluate the betung bamboo branch cutting response from various concentration of IBA and to know the best concentration of IBA to spur the growth.  The experiment was conducted in Palembang’s Bamboo Plantation Collection and Development Research Institute of Forest areal, Tanjung Agung, District Tanjungan, South Lampung Regency, from March to June 2012.  This research used Completely Randomized Design (CRD) with five treatments: IBA 0 ppm (control), 100 ppm, 200 ppm, 300 ppm, and 400 ppm of concentration.  Each of experimental unit consisted of five cuttings with five replications.  Percentage of life, length of shoot, number of shoot, number of leave, shoot’s diameter, and root’s length were the observed variables.  The result showed that betung bamboo branch cuttings were responsive to IBA, and IBA 200 ppm concentration gave better results to the betung bamboo branch cuttings than IBA 0 ppm (control), 100 ppm, 300 ppm, and 400 ppm concentration. Based on this study, it was recomended to use 200 ppm of IBA for betung bamboo branch cutting. Keywords : betung bamboo, branch cutting, indole butyric acid

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