scholarly journals The use of PCR assay for quality testing of grain of winter wheat cultivated in organic, integrated, conventional system and monoculture in phytopathological aspect

2012 ◽  
Vol 58 (2) ◽  
pp. 55-70 ◽  
Author(s):  
Aleksander Łukanowski ◽  
Czesław Sadowski
Keyword(s):  
Winter Wheat ◽  
Soil Science ◽  
Conventional System ◽  
Amplification Product ◽  
Pcr Assay ◽  
Organic System ◽  
Plant Cultivation ◽  
Gene Coding ◽  
Quality Testing

The aim of experiments was to evaluate the occurrence of fungi on grain of winter wheat cv. Roma cultivated in four systems on the experimental fields owned by the Institute of Soil Science and Plant Cultivation. Among pathogenic species, fungi from genus <i>Fusarium</i> dominated. Their number was the lowest on grain harvested in organic system and the highest in integrated one. Saprotrophic species were represented mainly by <i>Alternaria alternata</i>, which occurred the most often in organic system. Determination of <i>F. avenaceum</i>, <i>F. culmorum</i> and <i>F. poae</i> with microscope was confirmed with a PCR assay. All isolates of <i>F. culmorum</i> and <i>F. poae</i> gave an amplification product of <i>Tri 5</i> gene coding the possibility of trichocene production, while none of isolates of <i>F. avenaceum</i>.

Influence of a chemical preparation of triazole50% cs on sowing the winter wheat against rust

2020 ◽  
Vol 3 (9) ◽  
pp. 231-233
Author(s):  
AliyevSh.K. ◽  
TuychiyevI.U ◽  
Karimov N ◽  
Umaraliev.M.I
Keyword(s):  
Winter Wheat ◽  
Field Experiments ◽  
Yellow Rust ◽  
Biological Efficiency ◽  
Chemical Preparation ◽  
Seed Growth ◽  
Biological Farming ◽  
The Given ◽  
Farming Efficiency

The article is focused on the data of the carried works on studying biological efficiency of fungi Triazole 50% on sowing the winter wheat against yellow rust as well as on the height, development and fertility of the wheat. On May 5, 2019 from 9 to 10 o’clock under the temperature 21-23 field experiments of Triazol 50% CS manufactured by the firm “Agroximstar” (Uzbekistan) were carried out on winter wheat as a protector of seeds of winter wheat of Pervitsa sort against the disease of yellow rustin the irrigated conditions in an experimental field of the Institute “Istiklal” of Andijan district of Andijan region. The aim of the given research is to study biological-farming efficiency and determination of optimal norms of preparation expenses and to study the influence of fungicide on the height and development as well as on the fertility of the wheat. The received data showed that the preparation Triazole 50% CS effected on the pathogen of yellow rust favorably and besides that it didn’t effect on seed growth and energy of growth negatively.

DETECTING MYCOBACTERIUM TUBERCULOSIS RAPIDLY AND MYCOBACTERIUM TUBERCULOSIS STRAINS WITHOUT IS6110 SEQUENCE BY PCR ASSAY

2012 ◽  
pp. 15-19
Author(s):  
Thi Chau Anh Nguyen ◽  
Hoang Bach Nguyen ◽  
Hai Duong Huynh ◽  
Nu Xuan Thanh Le ◽  
Xuan Cuong Le ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
16S Rdna ◽  
False Negative ◽  
Clinical Samples ◽  
Tuberculosis Complex ◽  
Pcr Assay ◽  
Complex Objects ◽  
Method Performance ◽  
Gene Coding ◽  
16S Rdna Pcr

Background: The Nested IS6110 PCR is used for detecting tuberculosis, however IS6110 sequence is not present in the genome of all strains of M.tuberculosis, the result may be false negative. The gene coding 16S ribosome always contains a short sequence specific to M. tuberculosis complex. Objects: Performance of the 16S Real-time PCR to detect M. tuberculosis and combining to the nested IS6110 PCR to determine the rate of Mtb strains without IS6110 from clinical samples. Materials and method: Performance of 16S rDNA PCR by commercial kit of Viet A Inc. for all 480 samples, the samples which were positive with the 16S rDNA PCR were retested in IS6110 PCR assay by in-house kit. Results: The Realtime 16S rDNA PCR detected 258 cases (53.8%) of tuberculosis. There were 3 (1.2 %) M. tuberculosis strains which do not harbor IS6110 sequence in genome. Conclusion: The IS6110 nested PCR can be applied more widely than the 16S rDNA realtime PCR. In case of using IS6110 PCR assay, results may show a low proportion of false negative. Combining 16S rDNA PCR with the IS6110 based PCR allowed detection of deletion of IS6110 sequence in M. tuberculosis isolates.

A PCR assay for sex determination of yak (Bos grunniens) meat by amplification of the male-specific SRY gene

Food Control ◽  
2010 ◽  
Vol 21 (5) ◽  
pp. 726-731 ◽  
Author(s):  
W.L. Bai ◽  
R.H. Yin ◽  
S.J. Zhao ◽  
C. Li ◽  
Z.J. Ma ◽  
...  
Keyword(s):  
Sex Determination ◽  
Sry Gene ◽  
Pcr Assay ◽  
Bos Grunniens ◽  
Male Specific

scholarly journals The effectiveness of weed regulation methods in spring wheat cultivated in integrated, conventional and organic crop production systems

2012 ◽  
Vol 52 (4) ◽  
pp. 486-493 ◽  
Author(s):  
Beata Feledyn-Szewczyk
Keyword(s):  
Spring Wheat ◽  
Crop Production ◽  
Dry Matter ◽  
Production Systems ◽  
Integrated System ◽  
Integrated Systems ◽  
Conventional System ◽  
Weed Species ◽  
Organic System ◽  
Weed Communities

Abstract The research was conducted from 2008 to 2010, and compared the influence of different weed control methods used in spring wheat on the structure of the weed communities and the crop yield. The study was carried out at the Experimental Station of the Institute of Soil Science and Plant Cultivation - State Research Institute in Osiny as part of a long-term trial where these crop production systems had been compared since 1994. In the conventional and integrated systems, spring wheat was grown in a pure stand, whereas in the organic system, the wheat was grown with undersown clover and grasses. In the conventional system, herbicides were applied two times in a growing season, but in the integrated system - only once. The effectiveness of weed management was lower in the organic system than in other systems, but the dry matter of weeds did not exceed 60 g/m2. In the integrated system, the average dry matter of weeds in spring wheat was 4 times lower, and in the conventional system 10 times lower than in the organic system. Weed diversity was the largest in spring wheat cultivated in the organic system. In the conventional and integrated systems, compensation of some weed species was observed (Viola arvensis, Fallopia convolvulus, Equisetum arvense). The comparison of weed communities using Sorenson’s indices revealed more of a similarity between systems in terms of number of weed species than in the number of individuals. Such results imply that qualitative changes are slower than quantitative ones. The yield of grain was the biggest in the integrated system (5.5 t/ha of average). It was 35% higher than in the organic system, and 20% higher than in conventional ones.

scholarly journals Microbiological evaluation of lettuce produced by conventional and organic systems in farms of Londrina, PR

2017 ◽  
Vol 38 (1) ◽  
pp. 175 ◽  
Author(s):  
Natalia Harumi Niguma ◽  
Jacinta Sanchez Pelayo ◽  
Tereza Cristina Rocha Moreira de Oliveira
Keyword(s):  
Irrigation Water ◽  
Water Samples ◽  
Total Coliform ◽  
Organic Production ◽  
Conventional System ◽  
Salmonella Spp ◽  
Organic System ◽  
E Coli ◽  
Organic Systems ◽  
Production Practices

The aims of this study were to evaluate the contamination of lettuce (Lactuca sativa), produced in Londrina, Paraná (PR), with total coliform, coliform at 45 °C, E. coli, and Salmonella spp.; and to determine the E. coli contamination of irrigation water used at the farms studied. Four farms were evaluated, of which three produced lettuce using a conventional system and one using an organic system. An evaluation of the production practices of the farms was also carried out. A total of 111 samples were analyzed, 71 lettuce samples from the conventional system and 40 samples from the organic system. A total of eight irrigation water samples were collected for analysis. Coliform at 45 °C counts above the limit tolerated by Brazilian legislation were observed in 2.8% (2/71) of conventionally grown lettuce samples, and Salmonella spp. was isolated in 1.4% (1/71) of those samples. In the organic lettuce samples, 12.5% (5/40) had coliform at 45 °C counts above the limit tolerated and Salmonella spp. was not detected. Irrigation water samples from three farms were unsatisfactory, with counts higher than 102MPN of E. coli per 100mL. The results of this study demonstrate that most conventionally grown lettuce samples show good sanitary conditions in production, and that lettuce contamination is not related to contamination found in irrigation water samples. The results also showed that the organic production practices required by Brazilian certification agencies should be applied to ensure that contamination of produced lettuce remains controlled.

scholarly journals Postharvest quality of strawberry fruits produced in organic and conventional systems

2011 ◽  
Vol 29 (4) ◽  
pp. 577-583 ◽  
Author(s):  
Letícia KP Camargo ◽  
Juliano Tadeu V de Resende ◽  
Tânia T Tominaga ◽  
Sonia Maria Kurchaidt ◽  
Cristhiano K Camargo ◽  
...  
Keyword(s):  
Titratable Acidity ◽  
Physicochemical Characteristics ◽  
Soluble Solids ◽  
Postharvest Quality ◽  
Conventional System ◽  
Organic System ◽  
Camino Real ◽  
Sensorial Attributes ◽  
Solids Content ◽  
Conventional Systems

The aim of this study was to determine some physicochemical characteristics of fruits of eight strawberry cultivars (Aromas, Camino Real, Campinas, Dover, Oso Grande, Toyonoka, Tudla-Milsei and Ventana) cultivated in organic and conventional systems. The evaluated characteristics were soluble solids (SS), titratable acidity (TA), SS/AT ratio and anthocyanins. Evaluated sensory characteristics were flavor and appearance. The cultivars had a differential performance between themselves and among the systems for the evaluated characteristics. Generally, the conventional system produced fruit with higher levels of soluble solids and, organic system produced higher concentrations of anthocyanins. The fruits of the cultivar Toyonoka had the best soluble solids content in both systems and the anthocyanins content of 'Tudla-Milsei' and 'Camino Real' were proeminent in comparison to the other cultivars. The fruits from the organic system of production were preferred by judges on the sensorial attributes. The highest notes were given to the appearance of the cultivar Ventana in the conventional system and 'Tudla-Milsei' in the organic system.

scholarly journals Comparison of the Effect of Perennial Energy Crops and Arable Crops on Earthworm Populations

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 675 ◽  
Author(s):  
Feledyn-Szewczyk ◽  
Radzikowski ◽  
Stalenga ◽  
Matyka
Keyword(s):  
Winter Wheat ◽  
Crop Production ◽  
Crop Residues ◽  
Production Systems ◽  
Arable Land ◽  
Energy Crops ◽  
Integrated System ◽  
Species Number ◽  
Organic System ◽  
Perennial Energy Crops

The purpose of the study was to compare earthworm communities under winter wheat in different crop production systems on arable land—organic (ORG), integrated (INT), conventional (CON), monoculture (MON)—and under perennial crops cultivated for energy purposes—willow (WIL), Virginia mallow (VIR), and miscanthus (MIS). Earthworm abundance, biomass, and species composition were assessed each spring and autumn in the years 2014–2016 using the method of soil blocks. The mean species number of earthworms was ordered in the following way: ORG > VIR > WIL > CON > INT > MIS > MON. Mean abundance of earthworms decreased in the following order: ORG > WIL > CON > VIR > INT > MIS > MON. There were significantly more species under winter wheat cultivated organically than under the integrated system (p = 0.045), miscanthus (p = 0.039), and wheat monoculture (p = 0.002). Earthworm abundance was significantly higher in the organic system compared to wheat monoculture (p = 0.001) and to miscanthus (p = 0.008). Among the tested energy crops, Virginia mallow created the best habitat for species richness and biomass due to the high amount of crop residues suitable for earthworms and was similar to the organic system. Differences in the composition of earthworm species in the soil under the compared agricultural systems were proven. Energy crops, except miscanthus, have been found to increase earthworm diversity, as they are good crops for landscape diversification.

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