scholarly journals Micromorphology of flowers, anatomy and ultrastructure of Chamomilla recutita (L.) Rausch. (Asteraceae) nectary

2012 ◽  
Vol 64 (4) ◽  
pp. 23-34 ◽  
Author(s):  
Aneta Sulborska
Keyword(s):  
Glandular Trichomes ◽  
Single Layer ◽  
Apical Part ◽  
Vascular Bundles ◽  
Golgi Bodies ◽  
Transmission Electron ◽  
Cell Layers ◽  
Palisade Cells ◽  
Subcuticular Space ◽  
Nectariferous Tissue

Investigations of the micromorphology of flowers and the structure of nectaries in <i>Chamomilla recutita</i> L. (Rausch.) were carried out with the use of stereoscopic, light, scanning and transmission electron microscopy. Biseriate glandular trichomes consisting of 5-6 cell layers were found on the surface of the corollas of ray and disc florets. Accumulation of secretion within the subcuticular space was accompanied by degradation of trichome cells. Secretion release followed rupture of the cuticle in the apical part of the trichome. The ovary of the ray florets exhibited characteristic ribs covered with epidermis composed of radially elongated palisade cells. Nectariferous glands were present only in the disc florets. The ring-like nectary (93 × 163 µm; height × diameter) was located above the inferior ovary. The gland structure was formed by single-layer epidermis and 5-8 layers of specialised nectariferous parenchyma. Nectar was released via modified 15-20 µm wide stomata. The guard cells were slightly elevated above the surface of the other epidermal cells or were located slightly below them. The stomatal cells were characterised by small external and internal cuticular ledges. No vascular bundles were observed in the nectary. The gland was supplied by branches of vascular bundles reaching the style and ending at the nectary base. The nectariferous tissue was formed by isodiametric cells with a diameter of 11-20 µm. The cell interior was filled with electron dense cytoplasm containing a large nucleus, numerous pleomorphic plastids, mitochondria with a distinct system of cristae, Golgi bodies, ER profiles, and ribosomes. The plastid stroma was characterised by presence of pastoglobuli, intraplastid tubules, and lighter zones. Several small vacuoles were found in each cell. Plasmodesmata were visible in the walls of some cells. Lighter periplasmic space in which apoplastic transport of nectar might take place was observed between the plasmalemma and the cell wall. The presence of an osmiophilic substance in the intercellular spaces additionally corroborates this assumption.

scholarly journals The structure of the spur nectary in Dendrobium finisterrae Schltr. (Dendrobiinae, Orchidaceae)

2012 ◽  
Vol 64 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Magdalena Kamińska ◽  
Małgorzata Stpiczyńska
Keyword(s):  
Electron Microscopy ◽  
Cell Walls ◽  
Single Layer ◽  
Secretory Cells ◽  
Vascular Bundles ◽  
Nectar Guides ◽  
Floral Nectary ◽  
Transmission Electron ◽  
Secretory Tissue ◽  
The One

To date, the structure of the nectary spur of <i>Dendrobium finisterrae</i> has not been studied in detail, and the present paper compares the structural organization of the floral nectary in this species with the spurs of other taxa. The nectary spur of <i>D. finisterrae</i> was examined by means of light microscopy (LM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). It is composed of a single layer of secretory epidermis and several layers of small and compactly arranged subepidermal secretory cells. The secretory cells have thick cellulosic cell walls with primary pits. The secretory tissue is supplied by vascular bundles that run beneath in ground parenchyma and are additionally surrounded by strands of sclerenchymatous fibers. The flowers of the investigated species displayed morphological features characteristic of bee-pollinated taxa, as they are zygomorphic, creamy-green coloured with evident nectar guides. They also emit a weak but nice scent. However, they possess some characters attributed to bird-pollinated flowers such as a short, massive nectary spur and collenchymatous secretory tissue that closely resembles the one found in the nectaries of certain species that are thought to be bird-pollinated. This similarity in anatomical organization of the nectary, regardless of geographical distribution and phylogeny, strongly indicates convergence and appears to be related to pollinator-driven selection.

scholarly journals Morpho-Anatomy, Physiochemical and Phytochemical Standardization with HPTLC Fingerprinting of Aerial Parts of Trichosanthes lobata Roxb.

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
Patil Kalpana ◽  
Wadekar Raju
Keyword(s):  
Quantitative Estimation ◽  
Single Layer ◽  
Correct Identification ◽  
Vascular Bundles ◽  
Inorganic Elements ◽  
Aerial Parts ◽  
Different Types ◽  
Anomocytic Stomata ◽  
Palisade Cells

To study the morpho-anatomy of the aerial parts of Trichosanthes lobata (Cucurbitaceae) to increase the knowledge and standardization parameters of these plants. Morpho-anatomical studies of leaves have been carried out by free hand. The different types of histochemical test were performed by using staining reagents. Phytochemical and quantitative estimation has been determined along with HPTLC fingerprinting. Leaves simple, reniform, or ovate, 5 lobbed and glabrous. Anomocytic stomata are present on lower epidermis. A single layer of elongated palisade cells are present below upper epidermis. Leaf bears covering trichomes unicellular to 3-4 celled long. The central region is occupied by arc shaped vascular bundles comprising of xylem vessels surrounded with phloem cells. Physicochemical paradigms such as; ash value, inorganic elements, moisture content and extractive values were determined to develop stringent Pharmacognostic standards. Qualitative, and Quantitative standardization and, HPTLC fingerprint study of alcoholic and aqueous extracts confirmed the presence of quercitin as biomarker polyphenolic compound. These studies provides referential information for correct identification, as well as assessment of purity, quality of this plant, which definitely gaining the relevance in plant drug research and establishment of plant monograph.

scholarly journals Glandular trichomes of Robinia viscosa Vent. var. hartwigii (Koehne) Ashe (Faboideae, Fabaceae)—morphology, histochemistry and ultrastructure

Planta ◽  
2020 ◽  
Vol 252 (6) ◽  
Author(s):  
Agata Konarska ◽  
Barbara Łotocka
Keyword(s):  
Glandular Trichomes ◽  
Secretory Cells ◽  
Polyphenol Compounds ◽  
Urban Greenery ◽  
Stage Of Development ◽  
Transmission Electron ◽  
Secretory Products ◽  
Subcuticular Space ◽  
First Time ◽  
Anatomy And Ultrastructure

Abstract Main Conclusion Permanent glandular trichomes of Robinia viscosa var. hartwigii produce viscous secretion containing several secondary metabolites, as lipids, mucilage, flavonoids, proteins and alkaloids. Abstract Robinia viscosa var. hartwigii (Hartweg’s locust) is an ornamental tree with high apicultural value. It can be planted in urban greenery and in degraded areas. The shoots, leaves, and inflorescences of this plant are equipped with numerous persistent glandular trichomes producing sticky secretion. The distribution, origin, development, morphology, anatomy, and ultrastructure of glandular trichomes of Hartweg's locust flowers as well as the localisation and composition of their secretory products were investigated for the first time. To this end, light, scanning, and transmission electron microscopy combined with histochemical and fluorescence techniques were used. The massive glandular trichomes differing in the distribution, length, and stage of development were built of a multicellular and multiseriate stalk and a multicellular head. The secretory cells in the stalk and head had large nuclei with nucleoli, numerous chloroplasts with thylakoids and starch grains, mitochondria, endoplasmic reticulum profiles, Golgi apparatus, vesicles, and multivesicular bodies. Many vacuoles contained phenolic compounds dissolved or forming various condensed deposits. The secretion components were transported through symplast elements, and the granulocrine and eccrine modes of nectar secretion were observed. The secretion was accumulated in the subcuticular space at the trichome apex and released through a pore in the cuticle. Histochemical and fluorescence assays showed that the trichomes and secretion contained lipophilic and polyphenol compounds, polysaccharides, proteins, and alkaloids. We suggest that these metabolites may serve an important function in protection of plants against biotic stress conditions and may also be a source of phytopharmaceuticals in the future.

Micro-Structural Stability of Micropropagated Plants of Vitex negundo L.

2021 ◽  
pp. 1-9
Author(s):  
M. Manokari ◽  
S. Priyadharshini ◽  
Mahipal S. Shekhawat
Keyword(s):  
Structural Changes ◽  
Growth Conditions ◽  
Culture Industry ◽  
Vascular Bundles ◽  
Vitex Negundo ◽  
Structural Variations ◽  
Structural Repair ◽  
Micropropagated Plants ◽  
Palisade Cells

Abstract Micropropagation techniques allow producing large numbers of clones of genetically identical plants. However, there is evidence of disorders in internal structures due to sophisticated in vitro conditions. Such variations are responsible for the mortality of plantlets in the field and cause huge loss to the tissue culture industry. Anatomical evaluation at different growth conditions allows for understanding structural repair of in vitro raised plantlets. Therefore, the present study was aimed to identify the structural changes that occurred in micropropagated plants of Vitex negundo under heterotrophic, photomixotrophic, and photoautotrophic conditions. To achieve this, structural variations were analyzed in the plantlets obtained from in vitro, greenhouse and field transferred stages using light microscopy. Underdeveloped dermal tissues, palisade cells, intercellular spaces, mechanical tissues, vascular bundles, and ground tissues were observed with the plants growing under in vitro conditions. The self-repairing of structural disorders and transitions in vegetative anatomy was observed during hardening under the greenhouse environment. Field transferred plantlets were characterized by well-developed internal anatomy. These findings showed that the micropropagated plantlets of V. negundo were well-adapted through a series of self-repairing the in vitro induced structural abnormalities at the subsequent stages of plant development.

Ultrastructure of the female reproductive organs (ovary, vitellaria, and Mehlis' gland) of Halipegus eccentricus (Trematoda: Derogenidae)

1986 ◽  
Vol 64 (10) ◽  
pp. 2203-2212 ◽  
Author(s):  
Jon M. Holy ◽  
Darwin D. Wittrock
Keyword(s):  
Cell Types ◽  
Reproductive Organs ◽  
Golgi Body ◽  
Digenetic Trematode ◽  
Secretory Cells ◽  
Cortical Granules ◽  
Golgi Bodies ◽  
Transmission Electron ◽  
Vitelline Cells ◽  
Female Reproductive Organs

The female reproductive organs (ovary, vitellaria, and Mehlis' gland) of the digenetic trematode Halipegus eccentricus were studied by transmission electron microscopy. Oocytes entered diplotene while in the ovary and produced cortical granules and lipid bodies. Vitelline cells produced large amounts of eggshell protein but no yolk bodies. Two types of Mehlis' gland secretory cells were present, distinguishable by the morphology of their rough endoplasmic reticulum, Golgi bodies, and secretory bodies, and by the persistence of recognizable secretory material within the ootype lumen after exocytosis. In an attempt to standardize the nomenclature regarding the cell types of the Mehlis' gland, a classification that takes into account these four criteria is proposed. Two basic types of Golgi body organization were noted for the cells of the female reproductive system: a stack of flattened cisternae (Mehlis' gland alpha cells) and spherical Golgi bodies with vesicular cisternae (oocytes, vitelline cells, and Mehlis' gland beta cells).

scholarly journals Morphology and anatomy of leaf mine in Richterago riparia Roque (Asteraceae) in the campos rupestres of Serra do Cipó, Brazil

2002 ◽  
Vol 62 (1) ◽  
pp. 179-185 ◽  
Author(s):  
G. F. A. MELO DE PINNA ◽  
J. E. KRAUS ◽  
N. L. de MENEZES
Keyword(s):  
Host Plant ◽  
Vascular System ◽  
Glandular Trichomes ◽  
Protective Layer ◽  
Vascular Bundles ◽  
Campos Rupestres ◽  
Structural Alterations ◽  
Serra Do Cipó ◽  
Phenolic Substances

The leaf mine in Richterago riparia is caused by a lepidopteran larva (lepidopteronome). The leaves of R. riparia show campdodrome venation; the epidermis is unistratified, with stomata and glandular trichomes in adaxial and abaxial surfaces. The mesophyll is bilateral and the vascular system is collateral. During the formation of the mine, the larva consumes the chlorenchyma of the mesophyll and the smaller vascular bundles (veins of third and fourth orders). Structural alterations in the tissues of the host plant were not observed, except for the formation of a wound meristem and the presence of cells with phenolic substances next to the mine. Three cephalic exuviae of the miner were found in the mesophyll. This lepidopteronome is parenchymatic and the epidermis remains intact, but forms a protective layer for the mining insect.

Microstructure of SrTiO3 Thin Films as Single Layer and Incorporated in Yba2Cu3O7-x/SrTiO3 Multilayers

1995 ◽  
Vol 401 ◽  
Author(s):  
L. Ryen ◽  
E. Olssoni ◽  
L. D. Madsen ◽  
C. N. L. Johnson ◽  
X. Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Thin Films ◽  
Transmission Electron Microscopy ◽  
Lattice Mismatch ◽  
Single Layer ◽  
Transmission Electron ◽  
Tilt Boundaries ◽  
Interfacial Dislocations ◽  
The Individual ◽  
Film Substrate

AbstractEpitaxial single layer (001) SrTiO3 films and an epitaxial Yba2Cu3O7-x/SrTiO3 multilayer were dc and rf sputtered on (110)rhombohedral LaAIO3 substrates. The microstructure of the films was characterised using transmission electron microscopy. The single layer SrTiO3 films exhibited different columnar morphologies. The column boundaries were due to the lattice mismatch between film and substrate. The boundaries were associated with interfacial dislocations at the film/substrate interface, where the dislocations relaxed the strain in the a, b plane. The columns consisted of individual subgrains. These subgrains were misoriented with respect to each other, with different in-plane orientations and different tilts of the (001) planes. The subgrain boundaries were antiphase or tilt boundaries.The individual layers of the Yba2Cu3O7-x/SrTiO3 multilayer were relatively uniform. A distortion of the SrTiO3 unit cell of 0.9% in the ‘001’ direction and a Sr/Ti ratio of 0.62±0.04 was observed, both in correspondence with the single layer SrTiO3 films. Areas with different tilt of the (001)-planes were also present, within each individual SrTiO3 layer.

scholarly journals Changes in Structure and Histochemistry of Glandular Trichomes ofThymus quinquecostatusCelak

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Ping Jia ◽  
Ting Gao ◽  
Hua Xin
Keyword(s):  
Essential Oil ◽  
Secretory Pathway ◽  
Development Process ◽  
Glandular Trichomes ◽  
Capitate Trichomes ◽  
Different Types ◽  
Distribution Structure ◽  
Distinctive Difference ◽  
Subcuticular Space ◽  
Three Components

The types, morphology, distribution, structure, and development process of the glandular trichomes on the leaves ofThymus quinquecostatusCelak had been investigated in this study. Two different types of glandular trichomes were determined in detail, namely, capitate trichomes and peltate ones. Besides, there were distinct differences on morphology, distribution, structure, and development process between the two kinds of trichomes. As the peltate trichome stepping into senium stage, it caved in the epidermis integrally, which was different from the capitate one. The secretion of the capitate trichome contained essential oil, polyphenols, and flavonoids, while, in addition to these three components, the secretion of the peltate one also contained acid polysaccharides. A distinctive difference was also seen in the secretory pathway of the secretion between the two types of trichomes. The secretion of capitate one was extruded through the cuticle of the head cell, but the secretion of the peltate one kept accumulating in the subcuticular space of the head cells until it was released by cuticle rupture.

scholarly journals Macroscopy and Microscopy Study of Scopraia dulcis L.

2018 ◽  
Vol 23 (1) ◽  
pp. 56
Author(s):  
Djoko Santosa ◽  
Subagus Wahyuono ◽  
Sugeng Riyanto ◽  
SM Widyastuti
Keyword(s):  
Glandular Trichomes ◽  
Principal Component ◽  
Morphological Diversity ◽  
Microscopy Study ◽  
Sampling Location ◽  
Scoparia Dulcis ◽  
Region Selection ◽  
Microscopy Analysis ◽  
Palisade Cells ◽  
Selection Of

A morphological diversity analysis of Scoparia dulcis by macroscopy and microscopy has been conducted. The aim of this research is to determine the morphological and anatomical character of S. dulcis which grow in Opak watershed, Special Region of Yogyakarta. Samples were taken from Sleman (Cangkringan, Ngemplak Kalasan, and Prambanan) and Bantul (Imogiri and Pundong) region. Selection of sampling location is based on altitude difference. The samples are the third leaf order from the tip of the stem. They were measured in length and width. Microscopy analysis was performed by observing the number of glandular trichomes, palisade cells in the epidermis and stomata in the upper and lower epidermis. The data obtained were analyzed using principal component analysis (PCA). The results show that based on morphological and anatomical assessment, there were found 3 clusters of S. dulcis, i.e. Cangkringan-Ngemplak-Prambanan, Kalasan, and Imogiri-Pundong cluster.

scholarly journals Use of morphological and anatomical characters to delimit varieties of Paliurus spina-christi Mill. (Rhamnaceae)

2018 ◽  
Vol 6 (2) ◽  
pp. 1-14
Author(s):  
Saleem E. Shahbaz ◽  
Nazar M. Shareef
Keyword(s):  
Stomatal Density ◽  
Vascular Bundles ◽  
Epidermal Layer ◽  
Adaxial Side ◽  
Anatomical Features ◽  
Northern Iraq ◽  
Anatomical Characters ◽  
Palisade Cells ◽  
Different Parts ◽  
Fruit Diameter

Introduction: Paliurus spina-christi Mill is a species with two varieties namely Paliurus spina-christi L. var. spina-christi and Paliurus spina-christivar. macrocarpa Beck native to mountains of Kurdistan, rarely growing in the upper plains of northern Iraq. Materials and Methods: A total of 15 plants from different parts of Kurdistan region were sampled. 30measurements for each characters of Leaf, Inflorescence, Flowers, Fruit, seeds, and leaf anatomical characters were measured for comparison between the two varieties. Results and Discussion: Fruit diameter of var. macrocarpa is significantly larger than fruit diameter of var. spina-christi. Most mean flower parts especially the ovary, in addition to seed size is larger in var. macrocarpa. Upper epidermal layer is always thicker than the lower epidermal layer for the same blade. The isobilateral mesophyll includes 2 layers of long palisade cells adaxially and 2-3 layers of short cells abaxially. Many vascular bundles are seen equally spaced in the mesophyll tissue.With the exception of the presence of simple hairs on the petiole adaxial side of the var. spina-cristi and the absence of these hairs from the same site of var. macrocarpa, all other anatomical features are considered to be of no taxonomic application. Stomatal density of the adaxial leaf side is up to 19 stomata per mm² in var. spina-christi while it is up to 38 stomata per mm² in var. macrocarpa. Conclusions: Both fruit diameter and the stomatal density of the adaxial leaf side constitute diagnostic characters for separating between the two varieties of Paliurus spina-christi Mill

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