scholarly journals The metabolism of aged seeds The formation of polyribosomes in the embryos of germinating rye grains of different viability

2014 ◽  
Vol 54 (4) ◽  
pp. 417-427
Author(s):  
Kazimierz Zalewski
Keyword(s):  
Seed Germination ◽  
Rna Synthesis ◽  
Seed Aging ◽  
Quantitative Changes ◽  
Aged Seeds

Changes during seed germination in the cytoplasmic ribosomal fraction, the formation of polysomes and RNA synthesis in rye grain embryos of different age, were studied. Quantitative changes in embryo ribosomes accompanied seed aging. As the viability of the grain decreased, monosomes constituted an increasing proportion of the total ribosome content. The observed fall in RNA synthesis is considered a symptom of the deteriorative processes involved in seed aging.

scholarly journals Cytokinins and urea derivatives stimulate seed germination in Lotus corniculatus L.

2007 ◽  
Vol 59 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Radomirka Nikolic ◽  
Nevena Mitic ◽  
Suzana Zivkovic ◽  
D. Grubisic ◽  
Mirjana Neskovic
Keyword(s):  
Seed Germination ◽  
Lotus Corniculatus ◽  
Urea Derivatives ◽  
Adenine Sulfate ◽  
Germination Potential ◽  
Aged Seed ◽  
Isopentenyl Adenine ◽  
Aromatic Cytokinins ◽  
Aged Seeds

We studied the effects of various cytokinins and urea derivatives on germination of aged seeds of in Lotus corniculatus L. The following substances were applied: N6-isoprenoid cytokinins (isopentenyl adenine and zeatin), adenine sulfate, N6-aromatic cytokinins (kinetin, benzyladenine) and their N9-ribosides, N-benzyl-9-(2- tetrahydropyranyl)adenine, and urea derivatives (diphenylurea, thidiazuron, and chloro-pyridyl phenylurea). With the exception of adenine sulfate, all cytokinins increased the percentage of seed germination up to twofold, depending on their kind and concentration. It is concluded that cytokinins may be among the missing factors in aged seeds of L. corniculatus contributing to the implementation of their full germination potential. They could be used to improve germination of both freshly harvested and aged seed samples, if necessary. .

scholarly journals The metabolism of aged seeds. The formation of polyribosomes in germinating field bean ( Vicia faba sp. minor) seeds of different ages

2014 ◽  
Vol 61 (2) ◽  
pp. 203-210
Author(s):  
Kazimierz Zalewski
Keyword(s):  
Dehydrogenase Activity ◽  
Vicia Faba ◽  
Rna Synthesis ◽  
Field Bean ◽  
Embryonic Axes ◽  
Uridine Incorporation ◽  
Different Ages ◽  
Seed Ageing ◽  
Aged Seeds

Total dehydrogenase activity and formation of polyribosomes in embryonic axes and cotyledons of field bean seeds from different harvest years were studied. <sup>3</sup>H-uridine incorporation experiments showed that seed ageing was accompanied by decreased capability for RNA synthesis and polyribosome formation. The embryonic axes of seeds with reduced vigor contained lower levels of polyribosomes.

scholarly journals Effect of hydropriming and seed aging on seed germination and biochemical characteristics of pinto bean (Phaseolus vulgaris) seed under salt stress

2018 ◽  
Vol 4 (2) ◽  
pp. 37-55
Author(s):  
Majid Ghanbari ◽  
Seyed Ali Mohammad Modarres-Sanavy ◽  
Ali Mokhtassi Bidgoli ◽  
Parniyan Talebi-Siah Saran ◽  
◽  
...  
Keyword(s):  
Salt Stress ◽  
Phaseolus Vulgaris ◽  
Seed Germination ◽  
Biochemical Characteristics ◽  
Seed Aging ◽  
Pinto Bean

scholarly journals Metabolism of aged seeds. Changes in rye embryo polyribosomes during seed aging

2014 ◽  
Vol 61 (3-4) ◽  
pp. 369-379
Author(s):  
Kazimierz Zalewski
Keyword(s):  
Dehydrogenase Activity ◽  
Ribosomal Proteins ◽  
Control Sample ◽  
Electrophoretic Analysis ◽  
Winter Rye ◽  
Control Embryo ◽  
Ribonucleic Acids ◽  
Seed Aging ◽  
Almost All ◽  
Aged Seeds

Germination, total dehydrogenase activity, ribosomal proteins and ribonucleic acids of embryos obtained from winter rye harvested in different years and of distinctly differing viability were studied. Diminishing viability was accompanied by declines in the amount of ribosome-bound mRNA and a drop in the intensity of its synthesis during germination. Electrophoretic analysis of control embryo (fully viable) ribosome proteins revealed 55 different bands: 10 acidic and 45 basic. The embryos of aged seeds had 43 different bands of which 8 were acidic, 35 basic and almost all of which had an altered electrophoretic mobility as compared with the control sample. As aging progressed, the percentages of lysine, histidine and arginine increased while those of tyrosine, methionine, proline and serine decreased.

ZmAGA1 Hydrolyzes RFOs Late during the Lag Phase of Seed Germination, Shifting Sugar Metabolism toward Seed Germination Over Seed Aging Tolerance

2021 ◽  
Vol 69 (39) ◽  
pp. 11606-11615
Author(s):  
Yumin Zhang ◽  
Dan Li ◽  
Lynnette M. A. Dirk ◽  
A. Bruce Downie ◽  
Tianyong Zhao
Keyword(s):  
Seed Germination ◽  
Sugar Metabolism ◽  
Lag Phase ◽  
Seed Aging

scholarly journals Analysis of Stored mRNA Degradation in Acceleratedly Aged Seeds of Wheat and Canola in Comparison to Arabidopsis

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1707
Author(s):  
Liang Zhao ◽  
Hong Wang ◽  
Yong-Bi Fu
Keyword(s):  
Aging Time ◽  
Fragment Size ◽  
Mrna Degradation ◽  
Recent Analysis ◽  
Seed Aging ◽  
Degradation Rates ◽  
Stable Conditions ◽  
Stored Mrna ◽  
Aging Conditions ◽  
Aged Seeds

Seed aging has become a topic of renewed interest but its mechanism remains poorly understood. Our recent analysis of stored mRNA degradation in aged Arabidopsis seeds found that the stored mRNA degradation rates (estimated as the frequency of breakdown per nucleotide per day or β value) were constant over aging time under stable conditions. However, little is known about the generality of this finding to other plant species. We expanded the analysis to aged seeds of wheat (Triticum aestivum) and canola (Brassica napus). It was found that wheat and canola seeds required much longer periods than Arabidopsis seeds to lose seed germination ability completely under the same aging conditions. As what had been observed for Arabidopsis, stored mRNA degradation (∆Ct value in qPCR) in wheat and canola seeds correlated linearly and tightly with seed aging time or mRNA fragment size, while the quality of total RNA showed little change during seed aging. The generated β values reflecting the rate of stored mRNA degradation in wheat or canola seeds were similar for different stored mRNAs assayed and constant over seed aging time. The overall β values for aged seeds of wheat and canola showed non-significant differences from that of Arabidopsis when aged under the same conditions. These results are significant, allowing for better understanding of controlled seed aging for different species at the molecular level and for exploring the potential of stored mRNAs as seed aging biomarkers.

scholarly journals The Effect of Hydro-Priming on Germination Characteristics, Seedling Growth and Antioxidant Activity of Accelerated Aging Wheat Seeds

2015 ◽  
Vol 47 (4) ◽  
pp. 41-48 ◽  
Author(s):  
E. Ghasemi ◽  
M. Goodarzian Ghahfarokhi ◽  
B. Darvishi ◽  
Z. Heidari Kazafi
Keyword(s):  
Electrical Conductivity ◽  
Peroxidase Activity ◽  
Dry Weight ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Seed Aging ◽  
Seedling Length ◽  
Normal Seedling ◽  
Mean Time ◽  
Aged Seeds

Abstract Seed aging is an important problem in developing countries which seeds stored in inappropriate condition. Delayed germination, reduced normal seedling percentage and changed antioxidant enzymes activity are some indications of aged seeds. Priming is a technique applied before planting and can improve seed characteristics especially under abiotic stress conditions. The main objective of the study was to evaluate aging effect on seed quality and to study the interaction between seed aging and seed priming. A factorial experiment based on completely randomized design with three replicates was conducted. The experimental treatments included cultivar, priming and aging. Results showed that seed aging reduced germination percentage, germination index, seedling length, normal seedling percentage, seedling dry weight, catalase and ascorbate peroxidase activity and increased the germination mean time and electrical conductivity of seeds. The highest germination percentage, germination index, seedling length, normal seedling percentage, seedling dry weight, catalase and ascorbate peroxidase activity and the minimum germination mean time and electrical conductivity of seeds were attained from hydro-priming treatment under non aged condition. Hydro-priming improved aged seeds quality and increased enzymes activity. Therefore, priming is a technique can be applied to improve aged seeds germination and seedling characteristics.

HEAT SHOCK TREATMENT CAN IMPROVE SOME SEED GERMINATION INDEXES AND ENZYME ACTIVITY IN PRIMED SEEDS WITH GIBBERELLIN OF MOUNTAIN RYE (SECALE MONTANUM) UNDER ACCELERATED AGING CONDITIONS

2013 ◽  
Vol 46 (4) ◽  
pp. 21-30
Author(s):  
O. Ansari ◽  
F. Sharif-Zadeh ◽  
A. Moradi ◽  
M.S. Azadi ◽  
E. Younesi
Keyword(s):  
Enzyme Activity ◽  
Heat Shock ◽  
Seed Germination ◽  
Accelerated Aging ◽  
Shock Treatment ◽  
Seed Vigor ◽  
Heat Shock Treatment ◽  
Significant Difference ◽  
Secale Montanum ◽  
Aged Seeds

ABSTRACT Seed priming with gibberellin (GA) enhances seed germination performance; but the quality of primed seeds in aging condition often reduces more than non-primed seeds. An experiment was conducted to evaluate the effect(s) of heat shock treatments on germination characteristics and enzyme activity of primed mountain rye (Secale montanum) seeds with gibberellin under accelerated aging. Heat shock treatments, can substantially decrease the speed of quality reduction of mountain rye (Secale montanum) primed seeds. In primed seeds with gibberellin, which has non-aged, the highest germination percentage (GP) and normal seedling percentage (NSP) was attained from heat shock treatment at 35°C for 3 h, also after 3 days aging, it was attained from heat shock treatment at 35°C for 3 h. After 3 days of aging the highest germination index (GI) was attained from unprimed seeds, but no significant difference with heat shock treatment at 35°C for 3 h. The minimum means time germination (MTG) was in heat shock treatment at 30°C for 3 h in non-aged seeds. After 3 days of aging, heat shock treatment reduce MTG as compared to the primed seeds. Heat shock treatment at 35°C for 3 h increased seed vigor index (SVI) as compared to the unprimed and primed seed in non-aged seeds and after 3 days aging. Seedling length (SL) increases with heat shock treatment at 30°C for 4 h in non-aged seeds as compared to the primed and unprimed seeds, but after 3 days of aging heat shock treatment except at 35°C for 3 h and 40°C for 4 h reduced SL as compared to the primed and unprimed seeds. Also, heat shock treatments increase some antioxidant enzymes [Catalase (CAT), Ascorbat peroxidase (APX)].

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