scholarly journals Inhibition of growth and sporulation of Penicillium expansum by extracts of selected Basidiomycetes

2014 ◽  
Vol 69 (4) ◽  
pp. 263-267 ◽  
Author(s):  
Teresa Florianowicz
Keyword(s):  
Antifungal Activity ◽  
Pleurotus Ostreatus ◽  
Mycelial Growth ◽  
Penicillium Expansum ◽  
Aqueous Extracts ◽  
Coprinus Comatus ◽  
Inhibition Of Growth

A screening of antifungal activity of 33 Basidiomycetes strains was realized with their extracts. The influence of aqueous extracts of fruit-bodies of the testeded fungi after addition of spores or hyphae of <em>Penicillium expansum</em> on growth and sporulation of the fitopathogen in vitro was estimated. The species: <em>Hydnum repandum</em> L.: Fr., <em>Pleurotus ostreatus</em> (Jacq.: Fr.) Kummer, <em>Coprinus comatus</em> (Műll Fr.) S.F. Gray, <em>Coprinus atramentarius</em> (Bull: Fr.) Fr. and <em>Lactarius turpis</em> (Weinm.) Fr. reduced the mycelial growth and inhibited sporulation of this pathogen.

scholarly journals Management of Alternaria leaf blight in tomato plants by mentha essential oil

2020 ◽  
Vol 56 (No. 3) ◽  
pp. 191-196
Author(s):  
Kahkashan Perveen ◽  
Najat A. Bokhari
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Mycelial Growth ◽  
Leaf Blight ◽  
In Vitro Test ◽  
Tomato Plants ◽  
Conidia Germination ◽  
Alternaria Leaf Blight

The essential oil obtained by the hydro-distillation of the leaves of Mentha arvensis Linnaeus was evaluated for its antifungal activity against the causal agent of the Alternaria blight of tomatoes, i.e., Alternaria alternata (Fries) Keissler. The antifungal activity of the mentha essential oil was assessed both in vitro and in vivo. The chemical composition of the mentha oil was also identified by GCMS analysis. The in vitro test revealed that the maximum inhibition in the mycelial growth (93.6%) and conidia germination (90.6%) was at the highest concentration (40 µL/mL), furthermore, it was found that the inhibition of the mycelial growth and conidia germination was dose dependent. The in vivo test proved that the application of the mentha essential oil (40 µL/mL) significantly increased the plant height (84.6%), fresh weight (81.5%) and dry weight (80.0%) when compared to the untreated tomato plants. The disease incidence was 3.5 in the untreated plants, while it was 0.93 for the mentha essential oil treated plants and was 0.08 in the carbendazim treated plants. The GC-MS analysis of the mentha essential oil identified 18 compounds in total, among which the percentage of menthol was the highest (69.2%). The mentha essential oil was successful in managing the Alternaria leaf blight in the tomato plants. Therefore, it can be explored further for the development of a natural fungicide.

scholarly journals In vitro and in vivo antifungal activity of natural inhibitors against Penicillium expansum

2013 ◽  
Vol 33 ◽  
pp. 40-46 ◽  
Author(s):  
Claudia Fieira ◽  
Francieli Oliveira ◽  
Rubens Perez Calegari ◽  
Alessandra Machado ◽  
Alexandre Rodrigo Coelho
Keyword(s):  
Antifungal Activity ◽  
Penicillium Expansum ◽  
Natural Inhibitors ◽  
In Vivo Antifungal Activity

scholarly journals Microwave irradiation enhances the in vitro antifungal activity of citrus by-product aqueous extracts against Alternaria alternata

2018 ◽  
Vol 53 (6) ◽  
pp. 1510-1517 ◽  
Author(s):  
Konstantinos Papoutsis ◽  
Quan V. Vuong ◽  
Len Tesoriero ◽  
Penta Pristijono ◽  
Costas E. Stathopoulos ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Microwave Irradiation ◽  
Alternaria Alternata ◽  
Aqueous Extracts ◽  
In Vitro Antifungal Activity

Antifungal activity in vitro of ethanol and aqueous extracts of leaves and branches of Flourensia spp. against postharvest fungi

2017 ◽  
Vol 107 ◽  
pp. 499-508 ◽  
Author(s):  
D. Jasso de Rodríguez ◽  
E. de J. Salas-Méndez ◽  
R. Rodríguez-García ◽  
F.D. Hernández-Castillo ◽  
M.L.V. Díaz-Jiménez ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Aqueous Extracts

scholarly journals Salicylic acid in the induction of resistance to beet seedling damping-off and antifungal activity against Fusarium sp., in vitro

2016 ◽  
Vol 37 (1) ◽  
pp. 67 ◽  
Author(s):  
Douglas Junior Bertoncelli ◽  
Sérgio Miguel Mazaro ◽  
Rita De Cacia Dosciatti Serrão Rocha ◽  
Nean Locatelli Dalacosta ◽  
Adriano Lewandowski ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Antifungal Activity ◽  
Mycelial Growth ◽  
Experimental Unit ◽  
Damping Off ◽  
Potato Dextrose Agar Medium ◽  
Seedling Length ◽  
Fusarium Sp ◽  
And Control

The damping off is the main disease that affects the beet crop during the seedling production. The aim of this study was to evaluate different salicylic acid (SA) concentrations for resistance induction against damping-off in beet seedling and its antifungal activity against Fusarium sp., in vitro condition. Treatment of beet seed was with SA solution by immersion during 5 minutes in the 0.5, 1.0, 1.5 and 2.0 mM concentrations and control (distilled water). It was used four replications with 20 cells by experimental unit. The experiment was carried out for 14 days in cultivate chamber with temperature (23 oC ± 2°C), lighting (12 hours photoperiod) and humidity (70% ± 10%) controlled. After this time, the germination, damping off incidence, seedling length and fresh mass matter weight were evaluated. It was evaluated also in the seedling tissue the phenylalanine ammonia-lyase (PAL), ?-1.3 glucanase and chitinase level enzymes. In the in vitro the SA was putted in PDA (potato-dextrose-agar) medium, where the Fusarium sp. mycelial growth was evaluated. The SA applied for seeds treatment didn’t had effect significant on damping off of beet seedlings, but it induced the activity of ?-1.3 glucanase enzyme, it being this higher in nine times when compared the treatment control. The SA acted in the Fusarium sp. in vitro control with fungitoxic action, suppressed mycelial growth in 28% if compared to control.

scholarly journals Estimation of the antifungal activity of some preparations against Diaporthe eres under in vitro conditions

2019 ◽  
Vol 72 (4) ◽  
Author(s):  
Ewa Dorota Król ◽  
Barbara Anna Abramczyk ◽  
Beata Zimowska ◽  
Ewa Dorota Zalewska
Keyword(s):  
Natural Products ◽  
Antifungal Activity ◽  
Mycelial Growth ◽  
Fruit Trees ◽  
Thiophanate Methyl ◽  
Natural Origin ◽  
Chemical Groups

Six fungicides from various chemical groups and two natural products, i.e., Biosept Active (grapefruit extract) and Beta-Chikol (chitosan) were tested in vitro against <em>Diaporthe eres</em> isolated from the shoots of fruit trees. The preparations were incorporated in PDA medium to provide final fungicide concentrations of 1, 10, and 100 g cm<sup>−3</sup>. Biosept Active concentrations of 0.05%, 0.075%, and 0.1%, and Beta-Chikol concentrations of 1%, 2%, and 2.5%, respectively. The antifungal activity of the preparations was evaluated based on mycelial growth of <em>D. eres</em> strains after 4 and 8 days of culture and changes in the morphological structures of the fungus. The highest antifungal activity was registered for thiophanate-methyl at all tested concentrations, followed by thiram, which showed the same activity but only at 100 g cm<sup>−3</sup>. Among the preparations of natural origin, Beta-Chikol was more effective against <em>D. eres</em> than Biosept Active. The effects achieved by the former preparation were comparable with those achieved by some of the most effective fungicides tested against <em>D. eres</em>.

scholarly journals In vitro investigation of the antioxidant and antimicrobial effects of hydro-alcoholic and aqueous extracts of Globularia alypum L.

2020 ◽  
Vol 7 (1) ◽  
pp. 46-58
Author(s):  
Lamia Kraza ◽  
Senoussi Mohammed Mourad ◽  
Youcef Halis
Keyword(s):  
Antifungal Activity ◽  
Quantitative Estimation ◽  
Diffusion Method ◽  
Contact Method ◽  
Aqueous Extracts ◽  
Antioxidant Power ◽  
Antimicrobial Effects ◽  
Globularia Alypum ◽  
In Vitro Investigation

AbstractThe aim of this work was to establish the antioxidant and antimicrobial activity of hydro-alcoholic and aqueous extracts prepared from the leaves of Globularia alypum L. The quantitative estimation of total polyphenols (by the Folin-Ciocalteu method) and total flavonoids (by the method of aluminium trichloride) showed that the aqueous and ethyl acetate extracts had the highest content of phenolic and flavonoid compounds. Moreover, the results of antioxidant power assessed by both method DPPH and phosphomolybdenum indicated that leaves extracts of G. alypum expressed a considerable activity. The evaluation of the antimicrobial effects, using the disc diffusion method from antibacterial screening and the direct contact method from the antifungal activity, indicated strong antibacterial and antifungal activity.

scholarly journals Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum

2017 ◽  
Vol 53 (No. 3) ◽  
pp. 134-143 ◽  
Author(s):  
Mohammadi Parisa ◽  
Tozlu Elif ◽  
Kotan Recep ◽  
Kotan Merve Şenol
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Mycelial Growth ◽  
Spore Production ◽  
Penicillium Digitatum ◽  
Broth Culture ◽  
Agrobacterium Radiobacter ◽  
Green Mould

Ten bacteria isolate (4 Bacillus subtilis, 2 Bacillus pumilus, 2 Bacillus cereus, 1 Bacillus megaterium, and 1 Agrobacterium radiobacter) were tested in vitro for antagonistic properties against Penicillium digitatum, the causal agent of citrus green mould. The effect of these bacteria was also observed on mycelial growth, spore germination, and spore production of the pathogenic fungus in broth culture. Extracellular enzyme activities of the bacteria were determined. According to the results of in vitro antagonistic tests and enzymes activities, the most promising bacteria were Bacillus subtilis and Agrobacterium radiobacter. These bacteria were tested for disease suppression on lemon fruits. In addition, these bacterial isolates also showed remarkable antifungal activity against the pathogen on lemon fruits. The results of this study showed that Bacillus subtilis and Agrobacterium radiobacter showed remarkable antifungal activity against the pathogen. Chitinase and glucanase enzyme activity of all the tested bacteria was positive. Protease enzyme activity was positive in all tested bacteria with the exception of Agrobacterium radiobacter. In addition, all bacteria inhibited mycelial growth and spore germination (except Agrobacterium radiobacter) of the fungus. Bacillus subtilis, Bacillus cereus, and Agrobacterium radiobacter inhibited spore production in broth culture. Bacillus subtilis and Agrobacterium radiobacter were tested on lemon fruits significantly reduced disease severity. Consequently, these isolates can be used as new biocontrol agents in controlling the post-harvest decay of citrus fruits caused by Penicillium digitatum.

scholarly journals Antimicrobial Activity of two Semisynthetic Triterpene Derivatives from Euphorbia Officinarum Latex Against Fungal and Bacterial Phytopathogens

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Amal Smaili ◽  
Noureddine Mazoir ◽  
Lalla Aicha Rifai ◽  
Tayeb Koussa ◽  
Kacem Makroum ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Mycelial Growth ◽  
Erwinia Amylovora ◽  
Antimicrobial Effect ◽  
Penicillium Expansum ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Verticillium Dahlia

This study evaluated the in vitro antimicrobial effect of 3β-acetoxy-norlup-20-one (1) and 3-chloro-4α,14α-dimethyl-5α-cholest-8-ene (2), triterpene derivatives from Euphorbia officinarum latex against fungal and bacterial phytopathogens. Results showed that although mycelial growth of several strains of Verticillium dahlia, and Fusarium oxysporum fsp. melonis and Penicillium expansum was affected only moderately, the two compounds were able to reduce highly conidia formation and germination, suggesting that they act as fungistatic compounds. Their antibacterial activity was tested against Pseudomonas syringae pv. syringae (Pss), P. syringae pv. tabacci (Pst), Erwinia amylovora (Ea) and Agrobacterium tumefaciens (At) using disc diffusion method. Results showed that compound 2 was more effective in inhibiting the growth of Pss, Pst and Ea than compound 1.

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