scholarly journals Novel Application of Immunomodulatory Mushroom Polysaccharide (β-Glucan) and Triterpenes for Diabetic Wound Care

2020 ◽  
Author(s):  
Shiu-Nan Chen ◽  
Yu-Sheng Wu ◽  
Sherwin Chen ◽  
Ya-Chin Chang ◽  
Chung-Lun Lu
Keyword(s):  
Wound Healing ◽  
Ganoderma Lucidum ◽  
Cellular Proliferation ◽  
Wound Care ◽  
Immunomodulatory Activity ◽  
Diabetic Patients ◽  
Medicinal Mushroom ◽  
Positive Effects ◽  
Inflammatory Status ◽  
Healing Wound

β-Glucan and triterpenes are two important derivative compounds from traditional medicinal mushroom, such as Ganoderma lucidum and Antrodia cinnamomea. β-glucan and triterpenes are considered to have immunoregulatory properties in disease treatment for long years. The immunoregulatory effects are usually activated through some transcription of pro-inflammatory genes and possess immunomodulatory activity. Difficulty in healing wound now is a common condition that occurred in diabetic patients, and the physiological hyperglycemic status of diabetic patients resulting in the wounds continue to produce an inflammatory response. Thus, we hope to use β-glucan and triterpenes for difficult wound healing that possess immunomodulatory activity on the wound micro-environment and stimulate the positive effects on healing. In this chapter, these two important derivative compounds from traditional medicinal mushroom were examined by diabetic mammal’s wound healing models. In these models, the skin wounds’ microenvironment is expected close to diabetic foot, suffering in hyperglycemic and inflammatory status. The results are clearly presented, with the immunomodulatory effects from mushroom β-glucan and triterpenes that involved in modulating the cell-mediated immune system to cause cellular proliferation and further to introduce healing performance of the chronic inflammation wounds.

scholarly journals EFEKTIFITAS ANTARA PERAWATAN LUKA DENGAN MENGGUNAKAN NaCl 0,9% DAN BETADIN TERHADAP PROSES PENYEMBUHAN LUKA POST OPERASI

2020 ◽  
Vol 7 (1) ◽  
pp. 782-788
Author(s):  
Sri Lestari ◽  
Kunidah Kunidah
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Healing Process ◽  
Care Patient ◽  
Sectio Caesarea ◽  
Wound Healing Process ◽  
P Value ◽  
Purposive Sampling ◽  
Healing Wound ◽  
Post Operation

Penyembuhan luka merupakan suatu proses yang kompleks karena berbagai kegiatan bio-seluler, bio-kimia terjadi berkesinambungan. Sifat penyembuhan pada semua luka sama, dengan variasinya bergantung pada lokasi, keparahan, dan luasnya cedera. Tujuan penelitian ini untuk mengetahui efektifitas antara perawatan luka menggunakan NaCl 0,9% dengan betadin terhadap proses penyembuhan luka post operasi pada pasien sectio caesarea. Penelitian ini merupakan jenis penelitian Quaisy Experimen. Dengan populasi seluruh pasien perawatan luka post operasi sectio caesarea yaitu 26, dan menggunakan teknik sampel purposive sampling dengan jumlah 13 responden setiap kelompok. Penelitian ini dilakukan di Rumah Sakit Ciremai Kota Cirebon. Hasil penelitian ini adalah perawatan luka post operasi sectio caesarea menggunakan NaCl 0,9% sebanyak 13 responden dengan proses penyembuhan luka ≤ 3 hari di dapatkan rata-rata 7,07. Dan hasil bivariate menunjukkan bahwa ada perbedaan antara efektifitas proses penyembuhan luka pada perawatan luka pasien post operasi sectio caesarea menggunakan NaCl 0,9% dengan nilai p value = 0,000 < (a=0,05).Kata kunci     : Proses penyembuhan luka, perawatan luka, NaCl 0,9%, Betadin ABSTRACTWound healing is a complex process because of the various activies of bio-chemical, bio-cell occurs continuosly. Wound healing properties on the all the same, with variations depending on the location, saverity, and the extent of the injury. The purpose of this reseach was to determine the effectiveness of wound care using NaCl 0,9 % with using betadin to wound healing process post operation on patients sectio caesarea. This research is a kind of the quaisy experimental. With a population of whole patients wound care post operatition sectio caesarea which is 26, and using the tecnique of purposive sampling with a total of 13 respondents per-group. The research was conducted at the ciremai hospital of cirebon city.The results of this research are wound care post operation sectio caesarea using NaCl 0,9% as much 13 respondents with the healing process of wound ≤ 3 days obtained of average 7,07. Bivariate and result showed the there is between the effectiveness of the process of wound healing in wound care patient post operation sectio caesarea using NaCl 0,9% with a value p = 0,000 < (a=0,05).Keywords      :     The process of wound healing, wound care, NaCl 0,9%, betadin

COMPLEMENTARY ALTERNATIVE MEDICINE ( CAM ) TO PROMOTE WOUND HEALING IN DIABETIC ULCERS PATIENT: A LITERATURE REVIEW

2020 ◽  
Author(s):  
Ashar Prima ◽  
Amzal Mortin Andas ◽  
Asmiana Saputri Ilyas
Keyword(s):  
Wound Healing ◽  
Literature Review ◽  
Alternative Medicine ◽  
English Language ◽  
Wound Care ◽  
Cost Effective ◽  
Complementary Alternative Medicine ◽  
Diabetic Patients ◽  
Diabetic Ulcer ◽  
Key Word

Diabetic ulcer is one of the main reasons of morbidity in diabetic patients, and it accounts for about 50% of non-traumatic amputations throughout the world. Wound dressing is an integral part in the management of diabetic ulcer. The Literature Review intends to analyze the effectiveness of natural ingredients as complementary alternative medicine used in wound care for diabetic ulcer patients. A Method used is critical review full-textof the last 10 years periods(2007-2017) in English language. The Database used is PubMed, ScienceDirect, EBSCOhost, dan SAGE Journal. Search strategies used PICO (Population, Intervention, Comparison, Outcome), key word :“Diabetic Ulcer” and “Wound Healing’ and “ Complementary Alternative Medicine”. The articles selected gradually and obtained 18 articles. The Result of the literature review found 4 natural ingredient that can be used as dressing in the treatment of diabetic ulcer. The used of dressings can be used as an option in diabetic wound care because it effectively promote in wound healing and more cost effective.

scholarly journals Rosiglitazone accelerates wound healing by improving endothelial precursor cell function and angiogenesis in db/db mice

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7815
Author(s):  
Guoliang Zhou ◽  
Xue Han ◽  
Zhiheng Wu ◽  
Qiaojuan Shi ◽  
Xiaogang Bao
Keyword(s):  
Wound Healing ◽  
Cell Viability ◽  
Insulin Signaling ◽  
Tube Formation ◽  
Precursor Cell ◽  
Diabetic Patients ◽  
Diabetic Mice ◽  
Positive Effects ◽  
And Migration ◽  
Endothelial Precursor Cell

Background & Aims Endothelial precursor cell (EPC) dysfunction is one of the risk factors for diabetes mellitus (DM) which results in delayed wound healing. Rosiglitazone (RSG) is a frequently prescribed oral glucose-lowering drug. Previous studies have shown the positive effects of RSG on ameliorating EPC dysfunction in diabetic patients. Interestingly, knowledge about RSG with regard to the wound healing process caused by DM is scarce. Therefore, in this study, we investigated the possible actions of RSG on wound healing and the related mechanisms involved in db/db diabetic mice. Methods Db/db mice with spontaneous glucose metabolic disorder were used as a type 2 DM model. RSG (20 mg/kg/d, i.g.,) was administered for 4 weeks before wound creation and bone marrow derived EPC (BM-EPC) isolation. Wound closure was assessed by wound area and CD31 staining. Tubule formation and migration assays were used to judge the function of the BM-EPCs. The level of vascular endothelial growth factor (VEGF), stromal cell derived factor-1α (SDF-1α) and insulin signaling was determined by ELISA. Cell viability of the BM-EPCs was measured by CCK-8 assay. Results RSG significantly accelerated wound healing and improved angiogenesis in db/db mice. Bioactivities of tube formation and migration were decreased in db/db mice but were elevated by RSG. Level of both VEGF and SDF-1α was increased by RSG in the BM-EPCs of db/db mice. Insulin signaling was elevated by RSG reflected in the phosphorylated-to-total AKT in the BM-EPCs. In vitro, RSG improved impaired cell viability and tube formation of BM-EPCs induced by high glucose, but this was prevented by the VEGF inhibitor avastin. Conclusion Our data demonstrates that RSG has benefits for wound healing and angiogenesis in diabetic mice, and was partially associated with improvement of EPC function through activation of VEGF and stimulation of SDF-1α in db/db mice.

The Evaluation of the Effects of Nanoemulsion Formulations Containing Boron and/or Zinc on the Wound Healing in Diabetic Rats

2020 ◽  
pp. 153473462096189
Author(s):  
Gulsah Gundogdu ◽  
Kemal Alp Nalci ◽  
Afife Busra Ugur Kaplan ◽  
Koksal Gundogdu ◽  
Tuba Demirci ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblast ◽  
Clinical Problem ◽  
Human Dermal Fibroblast ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Scratch Assay ◽  
Positive Effects ◽  
Droplet Sizes ◽  
Hdf Cells

Wound healing remains a challenging clinical problem, especially in the presence of diabetes. Diabetic patients have the impaired ability to fight infection and insufficient inflammatory response. The aim of this study was to evaluate the effects of boronophenylalanine (BFA) and/or Zn-containing nanoemulsion (NE) formulations on wound healing in diabetic rats. MTT and scratch assays were performed to evaluate the proliferative effects of BFA and/or Zn on human dermal fibroblast (HDF) cells and the migration of these cells, respectively. The BFA and/or Zn-NE were prepared, and the effects of NEs on wound healing in diabetic rats were evaluated by applying once a day for 14 days. MTT assay showed that 10 to 25 µM BFA and/or 50 µM Zn had very significant positive effects on cell proliferation. In the scratch assay, 10 µM BFA significantly increased the migration of HDF cell compared with control. The droplet sizes of all the NEs were <115 nm and their zeta potential values were in range of (−) 23.9 ± 2.356 to (−) 33.1 ± 1.438 mV. There was a significant reduction in the wound contraction values (%) of the groups treated with the BFA and/or Zn-NE on the 14th day compared with the untreated diabetic rats group. According to histopathological findings, wound healing was nearly complete in BFA and/or Zn-NE compared with untreated diabetic rats. Especially, the group treated with the NE containing the low concentration of BFA showed highly promising results in wound healing of diabetic rats within 14 days with complete epithelialization and the completely closed wound area.

scholarly journals mTOR inhibitor INK128 promotes wound healing by regulating MDSCs

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yi Li ◽  
Yujun Xu ◽  
Xinhan Liu ◽  
Xin Yan ◽  
Yue Lin ◽  
...  
Keyword(s):  
Wound Healing ◽  
High Glucose ◽  
Kinase Inhibitor ◽  
Mammalian Target Of Rapamycin ◽  
Suppressor Cells ◽  
Immunohistochemical Analysis ◽  
Mtor Signaling ◽  
Diabetic Patients ◽  
Promote Wound Healing ◽  
Healing Wound

Abstract Background Skin wounds in diabetic patients hardly recover. Accumulating evidence has shown that mammalian target of rapamycin (mTOR) pathway and myeloid-derived suppressor cells (MDSCs) are involved in inflammatory-related response. INK128 is a novel mTOR kinase inhibitor in clinical development. However, the exact roles of MDSCs and INK128 in healing wound of diabetic patients are unclear. Methods Mice models of normal, diabetic, and diabetic+INK128 were constructed. Bone marrow (BM)-derived macrophages and RAW264.7 cell line co-cultured with MDSCs, which were induced at different conditions. Flow cytometry, western blot, quantitative real-time PCR, and immunohistochemical analysis were performed. Results Diabetic mice (DM) had a slower recovery rate, thinner epidermis and dermis, and less blood vessels than those of normal mice. MDSCs were abnormally accumulated in DM, mTOR was activated in MDSCs of DM, and the cells were treated with high glucose. Moreover, mTOR signaling inhibitor INK128 could promote wound healing through reducing the MDSCs. MDSC function was disordered in DM and high-glucose environments, while INK128 could help retrieve their function. Furthermore, high glucose and other factors in DM could promote M-MDSC differentiation to M1 pro-inflammatory macrophage cells, thus inhibiting wound healing. The differentiation, which was dependent on mTOR signaling, could be reversed by INK128. Conclusion INK128 is potential to be developed as a clinical strategy to promote wound healing of diabetic patients.

scholarly journals Cutaneous wound healing: Current concepts and advances in wound care

2014 ◽  
Vol 47 (03) ◽  
pp. 303-317 ◽  
Author(s):  
Kenneth C Klein ◽  
Somes Chandra Guha
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Standard Of Care ◽  
Medical Community ◽  
Medical Society ◽  
Cutaneous Wounds ◽  
General Acceptance ◽  
Healing Wound ◽  
The Usa ◽  
Variable Success

ABSTRACTA non-healing wound is defined as showing no measurable signs of healing for at least 30 consecutive treatments with standard wound care.[1] It is a snapshot of a patient’s total health as well as the ongoing battle between noxious factors and the restoration of optimal macro and micro circulation, oxygenation and nutrition. In practice, standard therapies for non-healing cutaneous wounds include application of appropriate dressings, periodic debridement and eliminating causative factors.[2] The vast majority of wounds would heal by such approach with variable degrees of residual morbidity, disability and even mortality. Globally, beyond the above therapies, newer tools of healing are selectively accessible to caregivers, for various logistical or financial reasons. Our review will focus on the use of hyperbaric oxygen therapy (HBOT), as used at our institution (CAMC), and some other modalities that are relatively accessible to patients. HBOT is a relatively safe and technologically simpler way to deliver care worldwide. However, the expense for including HBOT as standard of care for recognized indications per UHMS(Undersea and Hyperbaric Medical Society) may vary widely from country to country and payment system.[3] In the USA, CMS (Centers for Medicare and Medicaid Services) approved indications for HBOT vary from that of the UHMS for logistical reasons.[1] We shall also briefly look into other newer therapies per current clinical usage and general acceptance by the medical community. Admittedly, there would be other novel tools with variable success in wound healing worldwide, but it would be difficult to include all in this treatise.

scholarly journals Effects of Local Use of Insulin on Wound Healing in Non-diabetic Patients

2017 ◽  
Vol 26 (2) ◽  
pp. 75-79 ◽  
Author(s):  
Mario Aurelio Martínez-Jiménez ◽  
Francisco Javier Valadez-Castillo ◽  
Jorge Aguilar-García ◽  
José Luis Ramírez-GarciaLuna ◽  
Francisco Israel Gaitán-Gaona ◽  
...  
Keyword(s):  
Wound Healing ◽  
Standard Care ◽  
Therapeutic Option ◽  
Controlled Trial ◽  
Diabetic Patients ◽  
Double Blind ◽  
Positive Effects ◽  
Significant Difference ◽  
Vessel Growth ◽  
Local Use

Background: Clinical trials have shown the positive effects of local insulin therapy in the formation of new vessels and fibrosis in acute and chronic diabetic wounds without major adverse effects. Objective: The aim of this study was to investigate the effects of local insulin use on wound healing in non-diabetic patients. Methods: A randomized, split-plot, double-blind, placebo-controlled trial was conducted. Ten non-diabetic patients with full-thickness acute wounds were recruited (5 due to trauma, 3 to burns, and 2 to pressure). All wounds received standard bedside treatment. Each wound was divided into 2 zones. One side received a standard care plus insulin, while the other received standard care plus injection of saline solution. A biopsy specimen was taken from both sites on days 0 and 14. The amount of blood vessel growth and the percentage of fibrosis were evaluated. Results: A significant difference in the number of new vessels was observed on the insulin-treated site (70.6 [29.21]) compared to saline only (26.5 [34.3]; P < .04). The percentage of fibrosis (insulin 34.7 [28.02] vs saline 27.8 [29.9]) showed no significant difference. No adverse events related to the study occurred. The clinical implications of this study are considerable in terms of the formation of blood vessels but not fibrosis. Conclusion: We suggest that local insulin administration is a safe therapeutic option for angiogenesis in wounds of non-diabetic patients.

scholarly journals mTOR inhibitor INK128 promotes wound healing by regulating MDSCs

2021 ◽  
Author(s):  
Yi Li ◽  
Yujun Xu ◽  
Xinghan Liu ◽  
Xin Yan ◽  
Yue Lin ◽  
...  
Keyword(s):  
Wound Healing ◽  
High Glucose ◽  
Kinase Inhibitor ◽  
Mammalian Target Of Rapamycin ◽  
Suppressor Cells ◽  
Immunohistochemical Analysis ◽  
Mtor Signaling ◽  
Diabetic Patients ◽  
Promote Wound Healing ◽  
Healing Wound

Abstract Background: Skin wounds in diabetic patients are hardly to recover. Accumulating evidence has shown that mammalian target of rapamycin (mTOR) pathway and myeloid-derived suppressor cells (MDSCs) are involved in inflammatory-related response. INK128 is a novel mTOR kinase inhibitor in clinical development. However, the exact roles of MDSCs and INK128 in healing wound of diabetic patients are unclear. Methods: Mice models of normal, diabetic, and diabetic+INK128 were constructed. Bone marrow (BM)-derived macrophages and RAW264.7 cell line co-cultured with MDSCs, which were induced at different conditions. Flow cytometry, western blot, quantitative real-time PCR, and immunohistochemical analysis were performed. Results: Diabetic mice (DM) had a slower recovery rate, thinner epidermal and dermal, and less blood vessels than those of normal mice. MDSCs were abnormally accumulated in DM and mTOR was activated in MDSCs of DM and the cells treat with high glucose. Moreover, mTOR signaling inhibitor INK128 could promote wound healing through reducing the MDSCs. MDSCs function was disordered in DM and high glucose environments, while INK128 could help retrieve their function. Furthermore, high glucose and other factors in DM could promote M-MDSCs differentiation to M1 pro-inflammatory macrophage cells, thus inhibiting wound healing. The differentiation, which was dependent on mTOR signaling, could be reversed by INK128. Conclusion: INK128 is potential to be developed as a clinical strategy to promote wound healing of diabetic patients.

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