scholarly journals Phytochemical Composition, Antibacterial, and Antibiofilm Activity of Malva sylvestris Against Human Pathogenic Bacteria

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Mahmoud Fathi ◽  
Maryam Ghane ◽  
Leila Pishkar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Diffusion Method ◽  
Antibiofilm Activity ◽  
Malva Sylvestris

Background: Considering the increased rate of microbial resistance to antibiotics and chemical side effects of antibiotics, there is a need for an alternative antimicrobial agent with fewer complications. Medicinal plants are rich resources of phytochemical compounds with antibacterial activity that could fight off this problem. Objectives: The aim of this research was to investigate the chemical composition, antimicrobial, and antibiofilm properties of Malva sylvestris on some pathogenic bacteria. Methods: Antibacterial effect of the extract was assessed by the well diffusion and broth microdilution methods against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli. The anti-biofilm property of the extract was also examined using the crystal violet assay. Finally, the chemical constituents and total phenols of the extract were determined by gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC), respectively. Results: The methanolic extract of M. sylvestris showed antimicrobial activity against all tested Gram-negative and Gram-positive strains by the agar well diffusion method. The minimum inhibitory concentration (MIC) of the extract ranged from 21.9 ± 0.1 to 51.9 ± 0.5 mg/mL against the tested microorganisms. In addition, the minimum bactericidal concentration (MBC) spanned from 43.7 ± 0.1 to 85.8 ± 0.3 mg/mL. The biofilm inhibitory concentration (BIC50) of the extract was found to be 40 - 87 mg/mL against the tested bacteria. Analysis of the extract by GC-MS indicated that the most abundant compounds were 1-heptacosanol (38.41%), 17-Pentatriacontene (19.78%), and 6,9,12,15-docosatetraenoic acid, methyl ester (8.08%). High-performance liquid chromatography confirmed the presence of apigenin (6.84 ppm) and salicylic acid (1.5 ppm) as phenolic compounds in M. sylvestris methanolic extract. Conclusions: The results of this study represent the high potency of M. sylvestris extract as a source of biologically-active compounds for the development of future phytotherapeutic products with antibacterial and antibiofilm activity.

scholarly journals Portrayal of Punica granatum L. peel extract through High Performance Liquid Chromatography and antimicrobial activity evaluation

2023 ◽  
Vol 83 ◽  
Author(s):  
M.S. Mahmood ◽  
A. Ashraf ◽  
S. Ali ◽  
A.B. Siddique ◽  
F. Asad ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Staph Aureus ◽  
E Coli ◽  
Peel Extract

Abstract Increasing trend in antimicrobial resistance and failure of chemically synthesized antibiotics lead to discover alternative methods for the treatment of bacterial infections. Various medicinal plants are in use traditionally and their active compounds can be further applied for treatment of bacterial diseases. This study was designed to determine the antibacterial activity of Punica granatum (P. granatum L.) (pomegranate) peel extract against Enterobacteriaceae [Escherichia coli (E. coli), Salmonella Typhimurium (S. Typhimurium) and Shigella Dysenteriae (S. Dysenteriae)] and gram-positive bacterium [Staphylococcus aureus (Staph aureus)]. Methanolic extract of P. granatum L. peel was prepared by Soxhlet apparatus method. Total flavonoid and phenolic contents from the extract were determined by High Performance Liquid Chromatography (HPLC). The antibacterial activity of P. granatum L. peel extract was evaluated through agar well diffusion method. HPLC showed the range of phenolics (gallic acid, caffeic acid, benzoic acid, cinnamic acid) and flavonoid compounds. The chemical structures of flavonoid and phenolics found in the methanolic extract of P. granatum L. peel have been reported for the first time. The methanolic peel extract (50 ul) of yellow P. granatum L. showed 26, 10, 10 and 9mm zones of inhibition (ZOI) against S. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. The methanolic extract of red P. granatum L. (100 ul) showed 27, 8, 12 and 15 mm ZOI against Staph. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. Highest ZOI was observed against Staph. aureus. Many of the bacteria studied in the present work may cause serious gastrointestinal infections, which can lead to hemorrhagic diarrhea in children. These infections can be life-threatening to young children and the elderly. There is an incentive to find alternative control measures, such as plant and herbal extracts, especially in lesser-developed countries where traditional antibiotics may not be readily available.

scholarly journals Senna leaves: component composition, pharmacological properties, standardization

2021 ◽  
Vol 24 (2) ◽  
pp. 53-65
Author(s):  
Anna A. Romanyuk ◽  
Dmitry V. Moiseev
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Component Composition ◽  
Biologically Active ◽  
Pharmacological Properties ◽  
Anthracene Derivatives ◽  
The Russian Federation ◽  
The Republic ◽  
Active Substances

Objective: generalization and analysis of scientific data on the component composition, pharmacological properties and standardization of senna leaves. Materials and methods. The article provides an overview of scientific publications in the journals of the search platforms Scopus and PubMed, as well as the databases of the Russian Science Citation Index. The analysis of the range of herbal medicinal products was carried out according to the data of the State Registers of Medicines of the Republic of Belarus, the Russian Federation and the Republic of Kazakhstan using content analysis, comparison and generalization of data. Results. In addition to the laxative effect, determined by the presence of anthracene derivatives in senna leaves, the complex of their biologically active substances has antimicrobial, hypoglycemic, antioxidant, antifungal and antitumor effects. Also, the chemical composition of the plant is represented by fatty acids, triglycerides, flavonoids, macro- and microelements. The analysis of medicinal herbal preparations based on senna leaves registered in the Russian Federation, the Republic of Belarus and the Republic of Kazakhstan has been carried out. The differences in the methods of their standardization, given in the normative documentation on quality, have been revealed. The scientific experience of using the method of high performance liquid chromatography for the determination of anthracene derivatives in senna leaves is generalized. Conclusion. The complex of biologically active substances of senna leaves exhibits various pharmacological effects, which, possibly, will expand the range of combined preparations based on them. The use of a modern and selective method of high-performance liquid chromatography in the analysis of senna leaves is a very promising direction in the field of standardization of this type of medicinal plant material.

Measurement of carbamazepine and its epoxide metabolite by high-performance liquid chromatography, and a comparison of assay techniques for the analysis of carbamazepine.

1977 ◽  
Vol 23 (12) ◽  
pp. 2283-2287 ◽  
Author(s):  
G W Mihaly ◽  
J A Phillips ◽  
W J Louis ◽  
F J Vajda
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Plasma Concentrations ◽  
Correlation Coefficients ◽  
High Performance Liquid Chromatographic ◽  
Biologically Active ◽  
Gas Liquid Chromatography ◽  
Carbamazepine Therapy ◽  
Regression Slopes

Abstract We describe a modified high-performance liquid-chromatographic method for the simultaneous analysis of carbamazepine andits biologically active metabolite, carbamazepine-10, 11-epoxide. Concentrations of both these compounds in the plasma of 35 epileptic patients receiving chronic carbamazepine therapy are presented. Concentrations of carbamazepine in plasma were related to those of carbamazepine-10, 11-epoxide (r - 0.495, P less than 0.05). Total daily doses of carbamazepine were better correlated with plasma concentrations of carbamazepine-10, 11-epoxide (r = 0.714, P less than 0.001) than of carbamazepine (r = 0.269, P greater than 0.05). Close correlations were found between results of the three assay procedures we used to measure plasma carbamazepine concentrations: high-performance liquid chromatography, gas-liquid chromatography, and enzyme immunoassay. Correlation coefficients exceeded 0.97 and regression slopes were near unity, indicating that all three procedures were individually specific for the quantification of plasma carbamazepine.

Thin-layer and high-performance liquid chromatography of biologically active agents

1986 ◽  
Vol 369 ◽  
pp. 218-221 ◽  
Author(s):  
H.S. Gatica ◽  
M.A. Frontera ◽  
O. Pieroni ◽  
M.A. Tomás ◽  
A E. Zúñiga
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
High Performance ◽  
Biologically Active

Metronidazole Concentrations in Human Plasma, Saliva, and Gingival Crevice Fluid after a Single Dose

1986 ◽  
Vol 65 (12) ◽  
pp. 1420-1423 ◽  
Author(s):  
M.A.C. Van Oosten ◽  
F.J.W. Notten ◽  
F.H.M. Mikx
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Single Dose ◽  
High Performance ◽  
Inhibitory Concentration ◽  
Human Volunteers ◽  
Agar Diffusion Assay ◽  
Micro Organisms ◽  
Diffusion Assay ◽  
Gingival Fluid

Metronidazole concentrations were estimated in four human volunteers after a single dose of 750 mg taken orally. Samples of blood, saliva, and gingival crevice fluid were collected before intake and during the following 24 hours. The concentrations of metronidazole in plasma and saliva were measured by high-performance liquid chromatography (HPLC). The concentrations in gingival fluid were estimated by a capillary agar-diffusion assay. The results of the metronidazole measurements as obtained by both methods were significantly correlated. The peak concentrations of metronidazole in plasma and saliva were in the same range, 8.7-13.8 μ g/mL, and similar concentrations were found in the gingival fluid samples. It is concluded that metronidazole taken orally has similar pharmacokinetics in both saliva and plasma, and that a single oral dose of 750 mg metronidazole leads to a concentration of the drug in the gingival crevice fluid that exceeds the minimal inhibitory concentration for most anaerobic oral micro-organisms.

scholarly journals PRODUCTION OF CAMPTOTHECIN FROM ENDOPHYTIC FUNGI AND CHARACTERIZATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND ANTICANCER ACTIVITY AGAINST COLON CANCER CELL LINE.

2018 ◽  
Vol 11 (3) ◽  
pp. 166 ◽  
Author(s):  
Aswini A ◽  
Soundhari C
Keyword(s):  
Colon Cancer ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cell Line ◽  
Cancer Cell ◽  
High Performance ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Biologically Active

 Objective: Scientists are showing increasing interest in studying fungal endophytes as sources of novel and biologically active compounds. The present study was attempted to isolate and characterize camptothecin (CPT) from endophytic fungus Aspergillus niger isolated from Indian Piper betel plant and detection of its anticancer potential on colon cancer cell line.Methods: The production of CPT was confirmed by high-performance liquid chromatography techniques. The effect of CPT on hematopoietic cell transplantation (HCT) colon cancer cell line was studied by MTT assay.Results: The amount of CPT which is isolated from A. niger was found to be around 0.175 mg/L. Significant death of HCT cells was observed and was non-toxic to normal vero cell line.Conclusion: Hence, CPT can be used as a potential lead compound in cancer research.

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