scholarly journals A new Myxobolus (Cnidaria: Myxosporea) infecting the ornamental catfish Corydoras schwartzi from the Purus River in Brazil

2020 ◽  
Author(s):  
Patrick D. Mathews ◽  
Rafael R.M. Madrid ◽  
Omar Mertins ◽  
Vera L.S. Rigoni ◽  
André C. Morandini
Keyword(s):  
Polar Capsule ◽  
Body Shape ◽  
Histological Analysis ◽  
Polar Filament ◽  
Ornamental Fish ◽  
Homogeneous Material ◽  
Frontal View ◽  
First Report ◽  
Moderate Number ◽  
Cyst Development

A new microscopic cnidarian is described, infecting five of thirty (16.6%) specimens of Corydoras schwartzi caught in the Purus River, State of Amazonas, Brazil. Histological analysis showed that cyst development occurred in the serosa layer of the intestine. Mature myxospores are ovoid in body shape in frontal view, 22.4 ± 0.3 μm in total length and 16.3 ± 0.1 μm in width. Internally, two aubergine-shaped, elongate symmetrical polar capsules occupy more than half the length of the spore, 14.3 ± 0.2 μm in length and 6.5 ± 0.1 μm in width. Ultrastructural analysis provided evidence of five polar filament coils inside the polar capsule and binucleated sporoplasm containing a moderate number of sporoplasmosomes. The valvogenic cells abutting each other form a sutural ridge and frequently a thin layer of homogeneous material separates the cells. The outer surface of the myxospore valves is smooth, with no evidence of formation of ridges in the valves. Immature myxospores in various stages of development were observed. This study is the first report of a myxosporean parasitizing C. schwartzi and the first report of a myxosporean infection in the intestine of an ornamental fish from South America.

scholarly journals Myxobolus platanus n. sp. (Myxosporea, Myxobolidae), a parasite of Mugil platanus Günther, 1880 (Osteichthyes, Mugilidae) from Lagoa dos Patos, RS, Brazil

2007 ◽  
Vol 59 (4) ◽  
pp. 895-898 ◽  
Author(s):  
J.C. Eiras ◽  
P.C. Abreu ◽  
R. Robaldo ◽  
J. Pereira Júnior
Keyword(s):  
Polar Capsule ◽  
Posterior Extremity ◽  
Polar Filament ◽  
Lateral View ◽  
Frontal View ◽  
Iodinophilous Vacuole

Myxobolus platanus n. sp. infecting the spleen of Mugil platanus Günther, 1880 (Osteichthyes, Mugilidae) from Lagoa dos Patos, Brazil is described The parasites formed round or slightly oval whitish plasmodia (about 0.05-0.1mm in diameter) on the surface of the organ. The spores were round in frontal view and oval in lateral view, 10.7µm (10-11) long, 10.8µm (10-11) wide and 5µm thick, and presented four sutural marks along the sutural edge. The polar capsules, equal in size, were prominent, surpassing the mid-length of the spore, and were oval with the posterior extremity rounded, and converging with their anteriorly tapered ends. They were 7.7µm (7-8) long and 3.8µm (3.5-4) wide. A small intercapsular appendix was present. The polar filament formed five to six coils obliquely placed to the axis of the polar capsule. No mucous envelope or distinct iodinophilous vacuole were found.

Melanization in albino mice transformed by introducing cloned mouse tyrosinase gene

Development ◽  
1990 ◽  
Vol 108 (2) ◽  
pp. 223-227 ◽  
Author(s):  
S. Tanaka ◽  
H. Yamamoto ◽  
S. Takeuchi ◽  
T. Takeuchi
Keyword(s):  
Transgenic Mice ◽  
Histological Analysis ◽  
Pigment Production ◽  
Flanking Sequence ◽  
First Report ◽  
Tyrosinase Gene ◽  
Flanking Region ◽  
Albino Mice ◽  
Successful Expression

We introduced a mouse tyrosinase minigene, mg-Tyrs-J, in which the authentic genomic 5′ non-coding flanking sequence was fused to a mouse tyrosinase cDNA, into fertilized egges of albino mice. Of the 25 animals that developed from the injected eggs, four mice exhibited pigmented hair and eyes. Histological analysis of the transgenic mice revealed that the melanogenesis was restricted to hair bulbs and eyes. These results suggest that this minigene encodes active tyrosinase protein and that its 5′ flanking region contains the sequences regulating expression of mouse tyrosinase gene. This is the first report of a successful expression of tyrosinase gene and of pigment production in transgenic mice.

Redescription of Myxidium sphaericum Thélohan, 1895 and Ceratomyxa beloneae Lubat et al., 1989 from the gall bladder of the garpike, Belone belone in the Adriatic Sea

2009 ◽  
Vol 54 (4) ◽  
Author(s):  
Ivona Mladineo ◽  
Tanja Šegvić ◽  
Ivana Bočina ◽  
Leon Grubišić
Keyword(s):  
Gall Bladder ◽  
Adriatic Sea ◽  
Polar Filament ◽  
Posterior Surface ◽  
Anterior Surface ◽  
Frontal View ◽  
Complex Species ◽  
Line Drawings ◽  
Belone Belone ◽  
Spore Length

AbstractThe garpike Belone belone (L.) is an epipelagic fish abundant in the Mediterranean, parasitized by two coelozoic myxosporidians in the gall bladder — Myxidium sphaericum Thélohan, 1895 and Ceratomyxa beloneae Lubat, Radujković, Marques et Bouix, 1989. In order to redescribe these intriguing and taxonomically complex species, whose original descriptions are limited only to line drawings based on light microscopy, we studied the morphology and ultrastructure of their vegetative and sporogenic stages. M. sphaericum has an oval to sigmoid or mild crescent shape in the frontal view, large conical polar capsules, opening at the spore end, in opposite direction of the spore length, with 8–9 coils of polar filament. C. beloneae has elongated and slightly arched valves; symmetrical and smooth, with the posterior surface almost straight and anterior surface mildly inclinated, having 5 coils of the polar filament.

scholarly journals Myxobolus opsaridiumi sp. nov. (Cnidaria: Myxosporea) infecting different tissues of an ornamental fish, Opsaridium ubangiensis (Pellegrin, 1901), in Cameroon: morphological and molecular characterization

2021 ◽  
Vol 733 ◽  
pp. 56-71
Author(s):  
Guy Benoit Lekeufack-Folefack ◽  
Armandine Estelle Tchoutezo-Tiwa ◽  
Jameel Al-Tamini ◽  
Abraham Fomena ◽  
Suliman Yousef Al-Omar ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Lateral View ◽  
Cyprinid Fish ◽  
Ornamental Fish ◽  
Histopathological Analysis ◽  
Frontal View ◽  
Blast Search ◽  
The City ◽  
Morphological And Molecular Characterization ◽  
Different Tissues

We report a new myxozoan, Myxobolus opsaridiumi sp. nov., infecting the ornamental fish Opsaridium ubangiensis (Pellegrin, 1901) collected from the Anga River near the city of Yaounde, Cameroon. Plasmodia were found in the skin, muscles and spleen. The overall prevalence of infection was 54.7% (288 parasitized fish out of 526 examined). The myxospores were ovoid to subspherical in frontal view and lenticular in lateral view. The valves were symmetrical and relatively thick, without edge markings. The myxospore measurements were 10.7 ± 0.14 (10–11.5) μm long, 9 ± 0.15 (8–10) μm wide and 6.2 ± 0.7 (5.6–7.2) μm thick. The two ovoid polar capsules were equal in size, converging and opening together at the anterior end, measuring 5 ± 0.07 (4.3–6.0) μm long and 2.7 ± 0.07 (2.2–3.0) μm wide. Polar filaments were coiled from 5 to 7 turns. Histopathological analysis revealed no inflammatory reaction associated with the infection. A BLAST search found that the newly obtained 18 rDNA sequence had a low sequence similarity with available sequences for Myxobolus on GenBank. A phylogenetical analysis based on ribosomal DNA partial sequences showed that M. opsaridiumi sp. nov. is closely associated with several species of Myxobolus infecting cyprinid fish.

scholarly journals Ocurrencia de nemátodos en ninfas de Ephemeroptera en una quebrada de bosque tropical lluvioso

Intropica ◽  
2016 ◽  
Vol 11 ◽  
pp. 67 ◽  
Author(s):  
Limarie Judith Reyes-Torres ◽  
Yazminne Meléndez-Torres ◽  
Alonso Ramírez
Keyword(s):  
Puerto Rico ◽  
Body Length ◽  
Aquatic Insects ◽  
Light Microscope ◽  
Body Shape ◽  
Feeding Strategy ◽  
Field Station ◽  
First Report ◽  
Factors Associated

Nematodes are common symbionts of aquatic insects. Here, we assessed the presence of nematodes in mayfly nymphs (Ephemeroptera), evaluated their prevalence in the population, and determined factors associated with nematode presence. Mayflies were collected (n = 130) from three stream habitats (riffles, pools, and boulders) using a D net, in Prieta stream, El Verde Field Station, Puerto Rico. Mayflies were dissected and nematode presence was determined under a light microscope (4 x and 10 x). Nematode prevalence was 50 %. Nematodes were not identified beyond Phylum level, but we were able to eliminate various groups as infective agents (Nematoda: Mermithidae and Nematomorpha: Gordiida). They were mostly found in the abdomen, head or thorax of mayflies. There were differences in infection among taxa, Neohagenulus was the group with the highest proportion of infection. Mayfly body shape, feeding strategy, or activity potentially explain differences in infection among taxa. There were no differences in infection among habitats, but mayflies were less abundant in riffles; and there was no relation between mayfly body length and the number of nematodes present. To our knowledge, this is the first report of a nematode present in mayfly nymphs in Puerto Rico.

scholarly journals Detection and genetic analysis of infectious spleen and kidney necrosis virus (ISKNV) in ornamental fish from non-clinical cases: First report from India

2020 ◽  
Author(s):  
Sabyasachi Pattanayak ◽  
Anirban Paul ◽  
Pramoda Kumar Kumar Sahoo
Keyword(s):  
Reference Strain ◽  
Phylogenetic Reconstruction ◽  
Ornamental Fish ◽  
Necrosis Virus ◽  
Genotype I ◽  
First Report ◽  
Ornamental Fish Trade ◽  
Poecilia Sphenops ◽  
Pterophyllum Scalare ◽  
Fish Trade

Infectious spleen and kidney necrosis virus (ISKNV), a type species of the genus Megalocytivirus, poses a threat to ornamental fish trade as most cases show nonspecific symptoms, thus making timely diagnosis challenging. Apparently health molly (Poecilia sphenops) and angelfish (Pterophyllum scalare) collected from two distinct geographic localities of India were screened for four genera under Iridoviridae, Megalocytivirus {ISKNV, turbot reddish body iridovirus (TRBIV) and red seabream iridovirus (RSIV); ranaviruses and Singapore grouper iridovirus; and Lymphocystivirus} through molecular approach. In total five numbers out of 17 samples (29.4%), ISKNV genome fragments were detected. A PCR assay using major capsid protein (MCP) gene was standardised to detect and differentiate infections within the Megalocytivirus genus, even without aid of sequencing. This forms the first report of ISKNV infection in ornamental fish from India. Sequence analysis of MCP gene showed that Indian isolate being 100% similar to the complete genome or reference strain of ISKNV. Phylogenetic reconstruction demonstrated the present strain belonging to ISKNV genotype I. Furthermore, structural stability of the MCP revealed this strain was more stable than ISKNV genotype II, RSIV and TRBIV at 25 degree C and pH 7.0. Thus a strong pan-India surveillance is recommended to reduce trade risk.

Ultrastructure and molecular phylogeny of Pleistophora hyphessobryconis (Microsporidia) infecting hybrid jundiara (Leiarius marmoratus × Pseudoplatystoma reticulatum) in a Brazilian aquaculture facility

Parasitology ◽  
2015 ◽  
Vol 143 (1) ◽  
pp. 41-49 ◽  
Author(s):  
ANDREW D. WINTERS ◽  
INGEBORG M. LANGOHR ◽  
MARCOS DE A. SOUZA ◽  
EDSON M. COLODEL ◽  
MAURO P. SOARES ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Developmental Stages ◽  
Polar Filament ◽  
Inflammatory Cells ◽  
Spore Wall ◽  
Ornamental Fish ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Dense Mass

SUMMARYA microsporidian infecting the skeletal muscle of hybrid jundiara (Leiarius marmoratus × Pseudoplatystoma reticulatum) in a commercial aquaculture facility in Brazil is described. Affected fish exhibited massive infections in the skeletal muscle that were characterized by large opaque foci throughout the affected fillets. Histologically, skeletal muscle was replaced by inflammatory cells and masses of microsporidial developmental stages. Generally pyriform spores had a wrinkled bi-layer spore wall and measured 4·0 × 6·0 µm. Multinucleate meronts surrounded by a simple plasma membrane were observed. The polar filament had an external membrane and a central electron dense mass. The development of sporoblasts within a sporophorous vesicle appeared synchronized. Ultrastructural observations and molecular analysis of 16S rDNA sequences revealed that the microsporidian was Pleistophora hyphessobryconis. This study is the first report of a P. hyphessobryconis infection in a non-ornamental fish.

Taxonomy of the catfish genus Pseudoplatystoma Bleeker  (Siluriformes: Pimelodidae) with recognition of eight species

Zootaxa ◽  
2007 ◽  
Vol 1512 (1) ◽  
pp. 1-38 ◽  
Author(s):  
URIEL ANGEL BUITRAGO–SUÁREZ ◽  
BROOKS M. BURR
Keyword(s):  
Amazon Basin ◽  
Body Shape ◽  
Color Pattern ◽  
Ornamental Fish ◽  
The Other ◽  
Monophyletic Group ◽  
Anatomical Features ◽  
Fish Market ◽  
Subsistence Fishing

The genus Pseudoplatystoma Bleeker consists of three species long recognized as: P. fasciatum (Linnaeus), P. tigrinum (Valenciennes), and P. corruscans (Spix & Agassiz), and five species recently recognized or described here: P. punctifer (Castelnau), P. reticulatum Eigenmann & Eigenmann, P. orinocoense n. sp., P. metaense n. sp., and P. magdaleniatum n. sp. The eight species form a monophyletic group with two clades that are supported by anatomical features (i.e., skeletal anatomy and myology). One clade (P. tigrinum and P. metaense) is restricted to the Orinoco and Amazon basins, and the other clade, comprised of the remaining six species, is found in the Guyanas, Orinoco, Amazon, and Paraná basins. The species are diagnosed on the basis of body shape, color pattern (e.g., bars, loops, and spots), skeletal anatomy, and vertebral numbers. Pseudoplatystoma punctifer and P. tigrinum) are sympatric in the Amazon Basin, P. metaense and P. orinocoense in the Orinoco Basin, and P. corruscans and P. reticulatum, are sympatric in the Paraná. Pseudoplatystoma magdaleniatum (Magdalena basin) and P. fasciatum (Guyanas) each occur as the only species of Pseudoplatystoma in their respective individual ranges. Pseudoplatystoma reticulatum may be sympatric with the two other species in the Amazon Basin, but we have no records of them being captured together in the mainstream or tributaries. All eight species are used as food in both commercial and subsistence fishing, and there is a moderate–sized ornamental fish market for the young and juveniles. A key to adults of the eight species is included.

scholarly journals PENAMBAHAN TEPUNG PUCUK Indigofera zollingeriana (Miquel, 1855) DALAM PAKAN UNTUK MENINGKATKAN KUALITAS WARNA IKAN SUMATRA Puntigrus tetrazone (Bleeker, 1855)

2019 ◽  
Vol 7 (2) ◽  
pp. 889
Author(s):  
Enggi Rizki Pratama ◽  
Berta Putri ◽  
Luki Abdullah ◽  
Indra Gumay Yudha ◽  
Dwi Mulyasih
Keyword(s):  
Experimental Design ◽  
Freshwater Fish ◽  
Body Shape ◽  
Ornamental Fish ◽  
Fish Feed ◽  
Leaf Meal ◽  
Color Quality ◽  
Randomized Design ◽  
Indigofera Zollingeriana ◽  
Completely Randomized Design

Tiger barb (Puntigrus tetrazona) is one of the ornamental freshwater fish which has unique body shape and color compare to another ornamental fish. Fish color quality is caused by chromatophore pigmen cells in the epidermis layer. Increasing of color quality in fish can be reach through the addition of indigofera leaf meal that contain carotenoid. The aim of this research to study the effect of addition meal of Indigoferazollingerianaleaf on colour quality and growth of tiger barb. The experimental design used completely randomized design with 5 treatments i.e 0%, 5%, 10%, 15%, 20% of I. zollingeriana meal added in fish feed. The results showed that the addition of I. zollingeriana meal could increase tiger barb color quality. The addition of 15% I. zollingeriana meal was the best treatment

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