scholarly journals Abscisic acid (ABA)-mediated inhibition of seed germination involves a positive feedback regulation of ABA biosynthesis in Arachis hypogaea L.

2010 ◽  
Vol 9 (11) ◽  
pp. 1578-1586 ◽  
Author(s):  
Hu Bo ◽  
Wan Xiao rong ◽  
Liu Xiao hui ◽  
Guo Dong liang ◽  
Li Ling
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Arachis Hypogaea ◽  
Positive Feedback ◽  
Feedback Regulation ◽  
Arachis Hypogaea L ◽  
Aba Biosynthesis

ABA-dependent sucrose regulation of antioxidant metabolism in wheat cultivars varying in ABA-sensitivity

Biologia ◽  
2015 ◽  
Vol 70 (2) ◽  
Author(s):  
Sandeep Kaur ◽  
Anil Kumar Gupta ◽  
Vikramjit Kaur Zhawar
Keyword(s):  
Antioxidant Enzymes ◽  
Abscisic Acid ◽  
Seed Germination ◽  
Culture Medium ◽  
Sodium Tungstate ◽  
Wheat Cultivars ◽  
Antioxidant Metabolism ◽  
Aba Sensitivity ◽  
Aba Biosynthesis

AbstractEffect of sucrose and its abscisic acid (ABA) dependence was studied for seed germination, growth and antioxidant related parameters in two wheat cultivars PBW343 (ABA-lesser sensitive) and C306 (ABA-higher sensitive) by using sucrose (Suc) with or without sodium tungstate (inhibitor for ABA biosynthesis) in culture medium. Exogenous Suc (at 1.5% and 3%) inhibited germination in both cultivars where ABA-dependency for such inhibitions was seen only under 3% sucrose in PBW343 and 1.5% sucrose in C306. Exogenous Suc at 1.5% stimulated growth, which was ABA-dependent in both cultivars. Exogenous Suc at 3% inhibited growth in both cultivars but such inhibitions were ABA-dependent only in PBW343. Suc supply (3%) decreased antioxidant enzymes and H

Transcriptomics of nine-cis-epoxycarotenoid dioxygenase 6 induction in imbibed seeds reveals feedback mechanisms and long non-coding RNAs

2017 ◽  
Vol 27 (4) ◽  
pp. 251-261 ◽  
Author(s):  
Khadidiatou Sall ◽  
David Hendrix ◽  
Taira Sekine ◽  
Yoshihiko Katsuragawa ◽  
Ryosuke Koyari ◽  
...  
Keyword(s):  
Gene Expression ◽  
Positive Feedback ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Feedback Regulation ◽  
Negative Effects ◽  
Conditional Expression ◽  
Non Coding Rnas ◽  
Altered Gene ◽  
Aba Biosynthesis

AbstractInduction of nine-cis-epoxycarotenoid dioxygenase 6 (NCED6), an abscisic acid (ABA) biosynthesis gene, alone is sufficient to suspend germination in testa-ruptured seeds, which are at the final step of germination. Molecular consequences of NCED6 induction in imbibed seeds were investigated by RNA sequencing. The analysis identified many unknown and uncharacterized genes that were up-regulated by NCED6 induction, in addition to the major regulators of ABA signalling. Interestingly, other NCEDs were up-regulated by NCED6 induction, suggesting that the major rate-limiting enzymes in the ABA biosynthesis pathway are subject to positive-feedback regulation. ZEAXANTHIN EPOXIDASE and ABSCISIC ALDEHYDE OXIDASE3, which function upstream and downstream of NCED, were also up-regulated in seeds by NCED6 induction, which suggests that the distinct layers of positive feedback loops are coordinately operating in the NCED6-induced seeds. SOMNUS (SOM), which was also up-regulated by NCED6 induction, was the major mediator of enhanced ABA signalling in NCED6-induced seeds. SOM exerted negative effects on GA biosynthesis, which also contributes to a high ABA–GA ratio and reinforces the suppressive state of germination. Besides these coding genes, long intergenic non-coding RNAs (lincRNAs) were also up-regulated upon NCED6 induction (termed N6LINCRs). Conditional expression of N6LINCR1 altered gene expression profiles in seeds. Twenty-six genes were up-regulated and 66 genes were down-regulated by the induction of N6LINCR1. These results suggest that some of N6LINCRs have a regulatory role in gene expression in seeds, which potentially contributes to the regulation of germination by ABA.

scholarly journals ENAP1 retrains seed germination via H3K9 acetylation mediated positive feedback regulation of ABI5

PLoS Genetics ◽  
2021 ◽  
Vol 17 (12) ◽  
pp. e1009955
Author(s):  
Bo Zhao ◽  
Likai Wang ◽  
Zhengyao Shao ◽  
Kevin Chin ◽  
Daveraj Chakravarty ◽  
...  
Keyword(s):  
Seed Germination ◽  
Histone Acetylation ◽  
Molecular Mechanism ◽  
Positive Feedback ◽  
Target Genes ◽  
Feedback Regulation ◽  
Binding Activity ◽  
Promoter Regions ◽  
Protein Levels ◽  
H3k9 Acetylation

Histone acetylation is involved in the regulation of seed germination. The transcription factor ABI5 plays an essential role in ABA- inhibited seed germination. However, the molecular mechanism of how ABI5 and histone acetylation coordinate to regulate gene expression during seed germination is still ambiguous. Here, we show that ENAP1 interacts with ABI5 and they co-bind to ABA responsive genes including ABI5 itself. The hypersensitivity to ABA of ENAP1ox seeds germination is recovered by the abi5 null mutation. ABA enhances H3K9Ac enrichment in the promoter regions as well as the transcription of target genes co-bound by ENAP1 and ABI5, which requires both ENAP1 and ABI5. ABI5 gene is directly regulated by ENAP1 and ABI5. In the enap1 deficient mutant, H3K9Ac enrichment and the binding activity of ABI5 in its own promoter region, along with ABI5 transcription and protein levels are all reduced; while in the abi5-1 mutant, the H3K9Ac enrichment and ENAP1 binding activity in ABI5 promoter are decreased, suggesting that ENAP1 and ABI5 function together to regulate ABI5- mediated positive feedback regulation. Overall, our research reveals a new molecular mechanism by which ENAP1 regulates H3K9 acetylation and mediates the positive feedback regulation of ABI5 to inhibit seed germination.

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