The in vitro antioxidant property of methanolic extract of Afzelia africana (Smith.)

The methanolic extract of Litsea glutinosa (Lauraceae) was evaluated for in vitro antioxidant activity by determination of hydrogen peroxide scavenging activity, total antioxidant capacity, assay of nitric oxide scavenging activity and reducing power test and in vivo antinociceptive effect in acetic acid induced writhing model in swiss albino mice. The results revealed the presence of pronounced antioxidant property as compared with ascorbic acid used as standard and a dose-dependent (250 and 500 mg/kg) analgesic effect. The antioxidant and antinociceptive properties observed seem to be in good accordance with the traditional uses of Litsea glutinosa. Bangladesh J. Sci. Ind. Res. 47(4), 401-406, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.14069

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Antioxidant and cytotoxic potential of methanol extract of Tabernaemontana divaricata leaves

International Current Pharmaceutical Journal ◽

10.3329/icpj.v1i2.9446 ◽

1970 ◽

Vol 1 (2) ◽

pp. 27-31 ◽

Cited By ~ 7

Author(s):

Nowshin Nowaz Rumzhum ◽

Md Mostafizur Rahman ◽

Md Khalequzzaman Kazal

Keyword(s):

Cytotoxic Effect ◽

Methanolic Extract ◽

Reducing Power ◽

Pharmaceutical Journal ◽

Antioxidant Property ◽

Positive Control ◽

Dependent Manner ◽

Tabernaemontana Divaricata ◽

Vincristine Sulphate

The methanolic extract obtained from the leaves of Tabernaemontana divericata (Family: Apocynaceae) was evaluated for in vitro antioxidant potential by determination of total antioxidant capacity, assay of nitric oxide scavenging activity and reducing power test. The extract was also screened for its cytotoxic effect using brine shrimp lethality bioassay. The results revealed potent antioxidant property in all antioxidant assays compared to the reference antioxidant, ascorbic acid in a dose dependent manner. Further, the methanolic extract of Tabernaemontana divericata showed significant cytotoxic effect (LC50: 3.12μg/ml) compared with positive control, Vincristine Sulphate (LC50: 0.331μg/ml).Key Words: Tabernaemontana divaricata; Apocynaceae; Antioxidant; Cytotoxicity.DOI: http://dx.doi.org/10.3329/icpj.v1i2.9446International Current Pharmaceutical Journal 2012, 1(2): 27-31

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IN VITRO ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF LEAF EXTRACTS OF GREWIA HETEROTRICHA MAST

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2016v8i4.15282 ◽

2016 ◽

Vol 8 (4) ◽

pp. 68 ◽

Cited By ~ 1

Author(s):

B. Usha ◽

K. C. Pushpalatha

Keyword(s):

Aqueous Extract ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Antioxidant Property ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Scavenging Activity ◽

Leaf Extracts

Objective: To investigate the presence of phytochemical components and to evaluate the in vitro antioxidant activity of pet. ether, chloroform, methanol and aqueous extracts of Grewia heterotricha mast leaves.Methods: The leaves of Grewia heterotricha mast were dried and extracts were prepared using a pet. ether, chloroform, methanol by soxhlet extraction method. The aqueous extract was prepared using distilled water by cold extraction method. The preliminary phytochemical analysis was carried out on aqueous, methanol, chloroform and pet. either leaf extracts of the plant using standard qualitative procedures. The total phenolic content (TPC) was estimated using modified Folin-Ciocalteau method, tannin content by Folin-Denis method and total flavonoids by aluminum chloride method. In vitro, antioxidant activities were evaluated by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, H2O2 scavenging activity and FRAP assay.Results: The preliminary phytochemical analysis revealed the presence of complex bioactive constituents like phenols, tannins, alkaloids, terpenoids, flavonoids, saponins, steroids, glycosides, coumarins, proteins and carbohydrates. Methanolic extract showed highest total phenolic content (87.58±2.52 mg CE/g) than aqueous extract (78.46±5.36 mg CE/g). Higher tannin content was found in the aqueous extract (148.0±8.96 mg TAE/g). Total flavonoids were highest in chloroform extract (314.9±25.06 mg QE/g) followed by aqueous (242.98±32.42 mg QE/g) and methanolic extract (217.0±18.32 mg QE/g) and lowest in a pet. ether extract (188.86±23.35 mg QE/g). The methanolic extract had shown very significant DPPH radical scavenging activity (IC50 98.95 µg/ml) and H2O2 scavenging activity (IC50110.1µg/ml) compared to the standard ascorbic acid. Higher reducing ability was observed in methanol extract (131.8±11.67 mg AE/g).Conclusion: The results obtained reveal that the leaves of Grewia heterotricha mast have potent antioxidant property. The observed activity may be associated with bioactive components like phenolics, flavonoids present in the leaf extracts and could have greater importance as therapeutic agents in oxidative stress-related degenerative diseases. Further studies are needed in order to purify bioactive compounds responsible for the antioxidant property.

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Evaluation of Antioxidant and Antinociceptive Properties of Methanolic Extract of Clerodendrum viscosum Vent.

Stamford Journal of Pharmaceutical Sciences ◽

10.3329/sjps.v4i1.8873 ◽

1970 ◽

Vol 4 (1) ◽

pp. 74-78 ◽

Cited By ~ 4

Author(s):

Md Mostafizur Rahman ◽

Nowshin N Rumzhum ◽

Kudrat-E-Khuda Zinna

Keyword(s):

Ascorbic Acid ◽

Methanolic Extract ◽

Antinociceptive Effect ◽

Reducing Power ◽

Antioxidant Property ◽

Swiss Albino Mice ◽

Albino Mice ◽

Clerodendrum Viscosum

The PDF for this article was updated on 18/11/2011.NN Rumzhum and K Zinna were added as authors on 18/11/2011.The methanolic extract of Clerodendrum viscosum vent. (Verbenaceae) was evaluated for in vitro antioxidant activity by determination of total antioxidant capacity, assay of nitric oxide scavenging activity and reducing power test and in vivo antinociceptive effect in acetic acid induced writhing model in swiss albino mice. The results revealed the presence of pronounced antioxidant property as compared with ascorbic acid used as standard and a dose-dependent (250 and 500 mg/kg) analgesic effect in Clerodendrum viscosum vent. The antioxidant and antinociceptive activities obtained seem to be in good accordance with the traditional uses of Clerodendrum viscosum. Key words: Clerodendrum viscosum; Verbenaceae; Antioxidant; Ascorbic acid; Antinociceptive; Swiss albino mice DOI: http://dx.doi.org/10.3329/sjps.v4i1.8873 SJPS 2011; 4(1): 74-78

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Phytochemical and Pharmacological Activity of Myristica fragrans Houtt (Myristicaceae)

INTERNATIONAL JOURNAL OF TOXICOLOGICAL AND PHARMACOLOGICAL RESEARCH ◽

10.25258/ijtpr.v9i01.9038 ◽

2017 ◽

Vol 9 (01) ◽

Author(s):

Md Arifur Rahman Chowdhury ◽

Manirujjaman . ◽

Md Mazedul Haq

Keyword(s):

Acetic Acid ◽

Phenolic Content ◽

Methanolic Extract ◽

Radical Scavenging ◽

Antioxidant Property ◽

Free Radical Scavenging ◽

Total Phenolic ◽

Writhing Test ◽

Myristica Fragrans ◽

Myristica Fragrans Houtt

Objective: Myristica fragrans Houtt commonly used as traditional medicine for alleviating of various disorders. The purpose of our study was to map out the in vitro antioxidant property and in vivo anti-hyperglycemic and analgesic effect of the methanolic extract of Myristica fragrans Houtt. (Seed and mace) (Myristicaceae) on Swiss albino mice. Methods: The processed powder of Myristica fragrans Houtt (seed and mace) were subjected to methanolic extraction by soxhlet filtration methods, and the desiccated extract was used for screening of antioxidant by DPPH free radical scavenging assessment as well as total phenolic content by using folin-ciocalteu reagent.Anti-hyperglycemic effect and analgesic action tested through alloxan induced antidiabetics test and acetic acid-tempted writhing test on mice. Results: In DPPH free radical scavenging assessment, free radicals neutralization expressed as % of inhibition 49.69±0.06% also by IC50 values as 68.43 µg/ml surmise middle level of antioxidant property. The total phenolic content expressed as 186.25 mg/g equivalent of gallic acid indicates, active phenolic content. Oral administration of 200 and 400 mg/kg of extract dose and reference drug vildagliptin (50 mg/kg) for the duration of the 4-day study period, and initiated % of inhibition the blood glucose level measured as 22.48%, 44.78% and 62.02% regard as the significant anti-hyperglycemic properties. The analgesic activity was investigated by using the acetic acid-induced writhing test in mice, at the dose of 200 mg/kg body and 400mg/kg weight, and resulting 50.4% and 68.10% correspondingly, which was considerably significant with a standard drug. Conclusion: The present study suggests that methanolic extract of seed and mace of Myristica fragrans Houtt can manage moderate oxidative stress as well as perform the painkilling action. Besides, prolong medication may enhance the new dimension of anti-hyperglycemic activity.

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α-Glucosidase Inhibition and Docking Studies of 5-Deoxyflavonols and Dihydroflavonols Isolated from Abutilon pakistanicum

Current Organic Chemistry ◽

10.2174/1385272823666191001224741 ◽

2019 ◽

Vol 23 (17) ◽

pp. 1857-1866

Author(s):

Munawar Hussain ◽

Zaheer Ahmed ◽

Shamsun N. Khan ◽

Syed A. A. Shah ◽

Rizwana Razi ◽

...

Keyword(s):

Methanolic Extract ◽

Docking Studies ◽

Hydroxyl Group ◽

Coumaric Acid ◽

In Vitro Activity ◽

Aerial Parts ◽

First Time ◽

Acid Esters ◽

Phenol Moiety

Three new 5-deoxyflavonoid and dihydroflavonoids 2, 3 and 4 have been isolated from the methanolic extract of Abutioln pakistanicum aerial parts, for which structures were elucidated explicitly by extensive MS- and NMR-experiments. In addition to these, 3,7,4′-trihydroxy-3′-methoxy flavonol (1) is reported for the first time from Abutioln pakistanicum. Compound 2 and 4 are p-coumaric acid esters while compounds 2–4 exhibited α-glucosidase inhibitory activity. Docking studies indicated that the ability of flavonoids 2, 3 and 4 to form multiple hydrogen bonds with catalytically important residues is decisive hence is responsible for the inhibition activity. The docking results signified the observed in-vitro activity quite well which is in accordance with previously obtained conclusion that phenol moiety and hydroxyl group are critical for the inhibition of α-glucosidase enzyme.

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Extraction, Chemical Composition, Antioxidant Property and In vitro Anticancer Activity of Silymarin from Silybum Marianum On Kb and A549 Cell Lines

Current Drug Discovery Technologies ◽

10.2174/1570163817666200827111127 ◽

2020 ◽

Vol 17 ◽

Author(s):

Mojgan Azadpour ◽

Mohammad Mehdi Farajollahi ◽

Ali Mohammad Varzi ◽

Pejman Hashemzadeh ◽

Hossein Mahmoudvand ◽

...

Keyword(s):

Anticancer Activity ◽

Cell Lines ◽

A549 Cell ◽

Hplc Analysis ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antioxidant Property ◽

Silybum Marianum ◽

Stable Free Radical

Introduction: This study aimed to evaluate the antioxidant property of silymarin (SM) extracted from the seed of Silybum marianum and its anticancer activity on KB and A549 cell lines following 24, 48, and 72 h of treatment. Methods: Ten grams of powdered S. marianum seeds were defatted using n-hexane for 6 hours and then extracted by methanol. The silymarin extracted of extraction components The extracted components of silymarin were measured by spectrophotometric assay and HPLC analysis. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, phenol content, total flavonoid content, and total antioxidant capacity were measured to detect the antioxidant properties of SM. The anticancer activity of the SM on cell lines evaluated by MTT. Results: In HPLC analysis, more than 50% of the peaks were related to silibin A and B. SM was reducedDPPH (the stable free radical) with a 50% inhibitory concentration (IC50) of 6.56 μg/ ml in comparison with butylated hydroxyl toluene (BHT), which indicated an IC50 of ~3.9 μg/ ml.The cytotoxicity effect of SM on the cell lines was studied by MTT assay. The cytotoxicity effect of the extracted silymarin on KB and A549 cell lines was observed up to 80 and 70% at 156 and 78 µg/ml, respectively. The IC50 value of the extracted SM on KB and A549 cell lines after 24 hours of treatment was seen at 555 and 511 µg/ml, respectively. Conclusion: Due to the good antioxidant and anticancer properties of the isolated silymarin, its use as an anticancer drug is suggested.

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Investigation of Oral Subacute Toxicity and In-Vitro Antioxidant Activity of Standardized Methanolic Extract of Quercus serrata Leaves

Current Bioactive Compounds ◽

10.2174/1573407216999201224150602 ◽

2020 ◽

Vol 16 ◽

Author(s):

Maibam Beebina Chanu ◽

Biseshwori Thongam ◽

Khumukcham Nongalleima ◽

Hans Raj Bhat ◽

Surajit Kumar Ghosh ◽

...

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Biochemical Parameters ◽

Methanolic Extract ◽

Reducing Power ◽

Quercus Serrata ◽

Control Group ◽

Blood Cell Count ◽

Toxicological Profile

Background: Quercus serrata Murray leaves have been used traditionally in the treatment of diabetes, dysmenorrhoea, inflammation and urinary tract infection. So, far no study had been reported on the toxicological profile and antioxidant properties of the plant. Objective: The present study was aimed to investigate the in-vivo toxicological profile and in-vitro antioxidant activities of the methanolic extract of standardized Quercus serrata leaves. Methods: Per-oral sub-acute toxicity study was performed in rats using three dose levels (200, 400 and 800 mg/kg b.w.) of the extract for 28-days. Control group received gum acacia suspended in water. Bodyweight was measured weekly. Biochemical parameters were analysed using the serum, the blood-cell count was done using whole blood. Pathological changes were also checked in highly perfused tissues. Further, in-vitro reducing power assay, nitric oxide scavenging assay, DPPH free-radical scavenging assay were performed to check the antioxidant activity of the extract. Results: There were no significant alterations in the blood-cell count and biochemical parameters analysed in the treatment group when compared with the normal control. Histopathology study of liver, kidney, pancreas, heart and brain revealed normal cellular architecture in the treatment groups alike the control group animals. Quercus serrata also showed a significant reduction of DPPH with IC50 4.48±0.254 µg/mL, in-vitro reducing power activity with IC50121.65±0.320 µg/mL and nitric oxide scavenging activity IC50 106.43±0.338 µg/mL. Conclusion: The above study showed that standardized methanolic extract of Quercus serrata leaves was safe after subacute oral administration in rats and has good antioxidant potential.

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