scholarly journals Effect of landfill leachate on germination of lettuce seeds (Lactuca sativa L.)

2017 ◽  
Vol 21 ◽  
pp. 67
Author(s):  
Heider Alves Franco ◽  
Monica Regina da Costa Marques ◽  
Clarisse Ferreira Braga ◽  
Allana De Souza Izidorio ◽  
Sérgio Thode Filho
Keyword(s):  
Lactuca Sativa ◽  
Landfill Leachate ◽  
Lettuce Seeds ◽  
Lactuca Sativa L

scholarly journals Preventing thermoinhibition in a thermosensitive lettuce genotype by seed imbibition at low temperature

2003 ◽  
Vol 60 (3) ◽  
pp. 477-480 ◽  
Author(s):  
Warley Marcos Nascimento
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
High Temperatures ◽  
Lactuca Sativa ◽  
Lettuce Seed ◽  
Temperature Dependent ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Lettuce Seed Germination ◽  
Dark Green

Lettuce (Lactuca sativa L.) seed germination is strongly temperature dependent and under high temperatures, germination of most of genotypes can be erratic or completely inhibited. Lettuce seeds of 'Dark Green Boston' (DGB) were incubated at temperatures ranging from 15° to 35°C at light and dark conditions. Other seeds were imbibed in dark at 20°; 25°; 30°; and 35°C for 8 and 16 hours and then transferred to 20 or 35°C, in dark. Seeds were also incubated at constant temperature of 20° and 35 °C, in the dark, as control. In another treatment, seeds were primed for 3 days at 15°C with constant light. DGB lettuce seeds required light to germinate adequately at temperatures above 25°C. Seeds incubated at 20°C had 97% germination, whereas seeds incubated at 35°C did not germinate. Seeds imbibed at 20°C for 8 and 16 hours had germination. At 35°C, seeds imbibed initially at 20°C for 8 and 16 hours, had 89 and 97% germination, respectively. Seeds imbibed at 25°C for 16 hours, germinated satisfactory at 35°C. High temperatures of imbibition led to no germination. Primed and non-primed seeds had 100% germination at 20°C. Primed seeds had 100% germination at 35°C, whereas non-primed seeds germinate only 4%. The first hours of imbibition are very critical for lettuce seed germination at high temperatures.

The effects of ultra-high diluted gibberellic acid on in vitro lettuce seed germination.

2021 ◽  
Vol 15 (4) ◽  
pp. 50-54
Author(s):  
Carolina Santos Barreto ◽  
Fortune Homsani ◽  
Nina C Barboza Da Silva ◽  
Carla Holandino
Keyword(s):  
Gibberellic Acid ◽  
Lactuca Sativa ◽  
Germination Rate ◽  
Petri Dish ◽  
New Drugs ◽  
Lettuce Seed ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Petri Dishes

Lettuce seeds bioassays have been used in many different tests such as: alellopathyc models; developing of new drugs; ecotoxicity tests. In most cases, lettuce (Lactuca sativa L., Asteraceae) has been used because of its sensitivity, simultaneous and rapid germination, reliability of germination percentage and homogeneity of seeds. The main goal was to evaluate the effects of ultra-high diluted gibberellic acid (GA3) on lettuce seeds germination and seedling growth. Experiment was performed using Petri dishes containing one disk of Whatman nº01 paper watered with 1ml of water. In each Petri dish 10 lettuce seeds(Lactuca sativa L.) cv Regina 500 were placed and 2ml of the different treatment solutions were add: GA33µmol, GA3 3CH (10-6), GA3 12CH (10-24), water 12CH and water (no dilution and succussion). One milliliter solutions were added every 2 days of experiment. The experiment was repeated twice and each one consisted in 5 Petri dishes per treatment (n=100). All seeds were maintained in germination incubator under controlled temperature (25°C) and photoperiod (16L/8D). The tested substances were prepared according to Brazilian Homeopathic Pharmacopoeia (Brazil, 2011). The experiment was blinded all the time. All seeds germinated at same time (2 days) and after 7 days the germination rate was the same in all treatments. Root was affected just by Water 12 CH, in which shown the longest length (4.59 cm) when compared with others treatments. Shoot length was higher where gibberellin was added in concentration upper then Avogrado’s number.

The high reducing sugar content during germination contributes to desiccation damage in lettuce (Lactuca sativa L.) radicles

1995 ◽  
Vol 5 (3) ◽  
pp. 145-149 ◽  
Author(s):  
P. van der Toorn ◽  
B. D. McKersie
Keyword(s):  
Hydroxyl Radicals ◽  
Lactuca Sativa ◽  
Sugar Content ◽  
Primary Source ◽  
Reducing Sugar ◽  
Amino Groups ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Lipid Aldehydes

AbstractAt the time of protrusion, radicles of lettuce seeds became susceptible to desiccation damage. Concomitant with radicle elongation, both the reducing sugar content and the amount of lipid-aldehydes increased. The role of the hydroxy- and lipid-aldehydes in browning reactions that occurred during desiccation and re-imbibition was analysed. It was concluded that both types of aldehydes reacted with amino-groups during desiccation and that the increase in lipid-aldehydes during germination could be due to the augmented reducing sugar content. Therefore, the high reducing sugar content in radicles of germinated lettuce seeds may be the primary source of hydroxyl radicals, and thereby, a principal cause of desiccation damage.

scholarly journals Fitoquímica e Alelopatia da Aroeira -Vermelha (Schinus terebinthifolius Raddi) na Germinação de Sementes

2021 ◽  
Vol 25 (1) ◽  
pp. 02-08
Author(s):  
Gislayne de Araujo Bitencourt ◽  
Cibele Caren Moraes Gonçalves ◽  
Artur Guerra Rosa ◽  
Deizeluci De Fátima Pereira Zanella ◽  
Rosemary Matias
Keyword(s):  
Lactuca Sativa ◽  
Color Change ◽  
Eucalyptus Camaldulensis ◽  
Allelopathic Effect ◽  
Alcoholic Extract ◽  
Schinus Terebinthifolius ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Randomized Design ◽  
Germination And Growth

Os compostos alelopáticos podem afetar o crescimento e inibir a germinação das espécies. O objetivo deste trabalho foi analisar a fitoquímica e o efeito alelopático do extrato das folhas de Schinus terebinthifolius Raddi na germinação e crescimento de plântulas de Lactuca sativa L. e, posteriormente, aplicar a metodologia em eucalipto vermelho (Eucalyptus camaldulensis). Os compostos químicos foram determinados em extrato alcoólico das folhas, comparados e contrastados observando a alteração de cor. As sementes de alface e eucalipto vermelho foram distribuídas em gerbox contendo papel filtro como substrato, umedecido com 7 mL do extrato aquoso das folhas frescas, nas seguintes concen trações: (0; 12,5; 25; 50; 75 e 100%). Para todas as análises e experimentos foram utilizados três repetições. Os gerbox foram distribuídos em um delineamento inteiramente casualizado, mantidos em câmara de germinação com temperatura de 20 °C para alface e 28°C para eucalipto e fotoperíodo de 12 horas. Durante o segundo e quinto dia foram realizadas contagens das sementes germinadas de alface. O período de avaliação do experimento foi de cinco dias para alface e 12 dias para eucalipto. Ao final, foram determinadas as porcentagens de germinação, comprimento da raiz e de parte aérea das plântulas. Os resultados demonstraram interferência negativa na germinação e crescimento de alface e eucalipto em função do aumento da concentração do extrato. Entre os aleloquímicos predominantes estão os compostos fenólicos e derivados, com destaque aos flavonoides, terpenos e heterosídeos cardioativos o que pode estar relacionados ao efeito alelopático negativo da aroeira. O uso da aroeira associado ao eucalipto deve ser feito com um manejo adequado.   Palavras-chave: Alface. Aleloquímicos. Anacardiaceae. Eucalipto. Efeito Alelopático.   Abstract Allelopathic compounds can affect growth and inhibit species germination. The objective of this work was to analyze the phytochemistry and the allelopathic effect of  extract of  Schinus terebinthifolius Raddi leaves on the  Lactuca sativa L. seedlings germination and growth and later apply the methodology in red eucalyptus (Eucalyptus camaldulensis). The chemical compounds were determined in the leaves alcoholic extract, compared and contrasted observing the color change. The lettuce and red eucalyptus seeds were distributed in a gearbox containing filter paper as a substrate, moistened with 7 mL of the fresh leaves aqueous extract in the following concentrations: (0; 12.5; 25; 50; 75 and 100%). For all analyzes and experiments, three repetitions were used. The gearbox was distributed in a completely randomized design, maintained in a germination chamber with a temperature of 20 °C for lettuce and 28 °C for eucalyptus and a photoperiod of 12 hours. Germinated lettuce seeds were counted during the second and fifth days. The experiment evaluation period was five days for lettuce and 12 days for eucalyptus. At the end, the germination percentages, root length and the seedlings aerial part were determined. The results showed negative interference in the germination and growth of lettuce and eucalyptus due to the extract increased concentration. The most frequent allelochemicals were flavonoids, terpenes, tannins, anthocyanins and heterosides, which are probably related to the negative allelopathic effect. The use of  aroeira associated with eucalyptus must be done with proper management.   Keywords: Lettuce, Allelochemicals Anacardiaceae. Eucalyptus. Allelopathic Effect.

scholarly journals DEVELOPMENT OF ELEMENTS OF TECHNOLOGY FOR PLANTING MATERIAL OF LETTUCE (LACTUCA SATIVA L.) ON VIRUS-FREE BASIS USING METHODS OF BIOTECHNOLOGY

2019 ◽  
pp. 22-26 ◽  
Author(s):  
O. B. Romanova ◽  
A. V. Soldatenko ◽  
O. A. Chichvarina ◽  
V. A. Akhramenko ◽  
O. V. Pavlova ◽  
...  
Keyword(s):  
Lactuca Sativa ◽  
Nutrient Medium ◽  
Culture Media ◽  
Time Interval ◽  
Planting Material ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Different Temperatures

The article presents the results of research on the production in vitro of regenerated plants from the seeds of cultivars of lettuce (Lactuca sativa L.) Emerald, Bouquet, Chameleon (FSBSI Federal Scientific Vegetable Center), susceptible of aspermia tomato (Tomato aspermy cucumovirus) – AsTV. Seeds of strongly susceptible to AsTV varieties of salad Chameleon and Bouquet were subjected to thermotherapy at different temperatures (37°C, 38°C, 40°C) for a different time interval (1, 3, 5, 7, 10 days) in dry form and when moistened. Marked varietal specificity during germination of seeds after thermotherapy. Thus, the greatest number of seedlings in the emerald variety was obtained after 5 days of thermotherapy (10.0±0), while the Bouquet variety had the best results after 3 days of thermotherapy (9.3±1.2) with moisture. After thermotherapy of dry seeds by 40°C plant material of cultivar Emerald was planted on solid and liquid culture media. The conditions of step sterilization of lettuce seeds for introduction into the culture in vitro were chosen: washing in 96% ethanol, then in 50% aqueous solution of "Whiteness" with the addition of Twin-20, after in sterile distilled water. The nutrient medium for germination of lettuce seeds was used: Gamborg B5 (2% sucrose, 7.0 g/l agar), and the liquid nutrient medium was of that composition. The obtained seedlings were cutted and transferred to medium MS (2% sucrose, 0.1 mg/l ha and 1 mg/l BAP, 3.0 g/l phytogel). The formed shoots for rooting were transferred to the MS medium (2% sucrose, 3.0 g/l phytogel). In the future, lettuce plants will be adapted in vivo and tested for the presence of tomato aspermia virus in the planting material.

scholarly journals ENVIRONMENTAL IMPACTS CAUSED BY RESIDUAL VEGETABLE OIL IN THE SOIL-PLANT SYSTEM

2017 ◽  
Vol 39 (3) ◽  
pp. 748
Author(s):  
Sérgio Thode Filho ◽  
Julieta Laudelina de Paiva ◽  
Heider Alves Franco ◽  
Daniel Vidal Perez ◽  
Monica Regina da Costa Marques
Keyword(s):  
Vegetable Oil ◽  
Lactuca Sativa ◽  
Clayey Soil ◽  
Plant Morphology ◽  
Physical Parameters ◽  
Radicle Growth ◽  
Plant System ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Potential Contaminant

Vegetable oils are widely used for food production at different levels: domestic, commercial or industrial. The estimated production of vegetable oil, in Brazil, is three billion liters per year. Only 2.5% of cooking oil waste (WCO) are recycled. This paper presents to evaluate the environmental impact of OVR in two Brazilian soils (sandy and loamy) and to estimate the terrestrial toxicity level (leakage test) and phytotoxicity (germination tests using lettuce seeds - Lactuca sativa L. – as well as in lettuce cultivation under greenhouse conditions). After leaching, the sandy soil had a slight lower WCO adsorption capacity than the clayey soil. The introduction of WCO caused clayey soil compaction. The WCO presence was toxic to earthworms, regardless of soil type. WCO negatively influenced germination and radicle growth in the eco toxicity assays. The different tested doses of WCO in lettuce (Lactuca sativa L.) cultivation caused changes in plant morphology for all physical parameters analyzed for both soils. We concluded that the WCO is a potential contaminant residue when improperly disposed over the soil, compromising the soil-plant system.

scholarly journals Evaluation of the impact of vegetable oils on the germination of lettuce seeds (Lactuca sativa L.)

2017 ◽  
Vol 21 ◽  
pp. 78
Author(s):  
Sérgio Thode Filho ◽  
Emanuele Nunes de Lima Figueiredo Jorge ◽  
Leonardo Corrêa de Souza ◽  
Matheus Lucas Rocha da Silva Pereira ◽  
Raiane De Almeida Marinho Nascimento ◽  
...  
Keyword(s):  
Vegetable Oils ◽  
Lactuca Sativa ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
The Impact
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