The effects of ultra-high diluted gibberellic acid on in vitro lettuce seed germination.

2021 ◽  
Vol 15 (4) ◽  
pp. 50-54
Author(s):  
Carolina Santos Barreto ◽  
Fortune Homsani ◽  
Nina C Barboza Da Silva ◽  
Carla Holandino
Keyword(s):  
Gibberellic Acid ◽  
Lactuca Sativa ◽  
Germination Rate ◽  
Petri Dish ◽  
New Drugs ◽  
Lettuce Seed ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Petri Dishes

Lettuce seeds bioassays have been used in many different tests such as: alellopathyc models; developing of new drugs; ecotoxicity tests. In most cases, lettuce (Lactuca sativa L., Asteraceae) has been used because of its sensitivity, simultaneous and rapid germination, reliability of germination percentage and homogeneity of seeds. The main goal was to evaluate the effects of ultra-high diluted gibberellic acid (GA3) on lettuce seeds germination and seedling growth. Experiment was performed using Petri dishes containing one disk of Whatman nº01 paper watered with 1ml of water. In each Petri dish 10 lettuce seeds(Lactuca sativa L.) cv Regina 500 were placed and 2ml of the different treatment solutions were add: GA33µmol, GA3 3CH (10-6), GA3 12CH (10-24), water 12CH and water (no dilution and succussion). One milliliter solutions were added every 2 days of experiment. The experiment was repeated twice and each one consisted in 5 Petri dishes per treatment (n=100). All seeds were maintained in germination incubator under controlled temperature (25°C) and photoperiod (16L/8D). The tested substances were prepared according to Brazilian Homeopathic Pharmacopoeia (Brazil, 2011). The experiment was blinded all the time. All seeds germinated at same time (2 days) and after 7 days the germination rate was the same in all treatments. Root was affected just by Water 12 CH, in which shown the longest length (4.59 cm) when compared with others treatments. Shoot length was higher where gibberellin was added in concentration upper then Avogrado’s number.

scholarly journals Preventing thermoinhibition in a thermosensitive lettuce genotype by seed imbibition at low temperature

2003 ◽  
Vol 60 (3) ◽  
pp. 477-480 ◽  
Author(s):  
Warley Marcos Nascimento
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
High Temperatures ◽  
Lactuca Sativa ◽  
Lettuce Seed ◽  
Temperature Dependent ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Lettuce Seed Germination ◽  
Dark Green

Lettuce (Lactuca sativa L.) seed germination is strongly temperature dependent and under high temperatures, germination of most of genotypes can be erratic or completely inhibited. Lettuce seeds of 'Dark Green Boston' (DGB) were incubated at temperatures ranging from 15° to 35°C at light and dark conditions. Other seeds were imbibed in dark at 20°; 25°; 30°; and 35°C for 8 and 16 hours and then transferred to 20 or 35°C, in dark. Seeds were also incubated at constant temperature of 20° and 35 °C, in the dark, as control. In another treatment, seeds were primed for 3 days at 15°C with constant light. DGB lettuce seeds required light to germinate adequately at temperatures above 25°C. Seeds incubated at 20°C had 97% germination, whereas seeds incubated at 35°C did not germinate. Seeds imbibed at 20°C for 8 and 16 hours had germination. At 35°C, seeds imbibed initially at 20°C for 8 and 16 hours, had 89 and 97% germination, respectively. Seeds imbibed at 25°C for 16 hours, germinated satisfactory at 35°C. High temperatures of imbibition led to no germination. Primed and non-primed seeds had 100% germination at 20°C. Primed seeds had 100% germination at 35°C, whereas non-primed seeds germinate only 4%. The first hours of imbibition are very critical for lettuce seed germination at high temperatures.

scholarly journals Effect of Gibberellic Acid, Kinetin, and Ethylene plus Carbon Dioxide on the Thermodormancy of Lettuce Seed (Lactuca sativa L. cv. Mesa 659)

1975 ◽  
Vol 56 (6) ◽  
pp. 826-829 ◽  
Author(s):  
Robert D. Keys ◽  
Orrin E. Smith ◽  
Junji Kumamoto ◽  
Jessye L. Lyon
Keyword(s):  
Carbon Dioxide ◽  
Gibberellic Acid ◽  
Lactuca Sativa ◽  
Lettuce Seed ◽  
Lactuca Sativa L

scholarly journals DEVELOPMENT OF ELEMENTS OF TECHNOLOGY FOR PLANTING MATERIAL OF LETTUCE (LACTUCA SATIVA L.) ON VIRUS-FREE BASIS USING METHODS OF BIOTECHNOLOGY

2019 ◽  
pp. 22-26 ◽  
Author(s):  
O. B. Romanova ◽  
A. V. Soldatenko ◽  
O. A. Chichvarina ◽  
V. A. Akhramenko ◽  
O. V. Pavlova ◽  
...  
Keyword(s):  
Lactuca Sativa ◽  
Nutrient Medium ◽  
Culture Media ◽  
Time Interval ◽  
Planting Material ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Different Temperatures

The article presents the results of research on the production in vitro of regenerated plants from the seeds of cultivars of lettuce (Lactuca sativa L.) Emerald, Bouquet, Chameleon (FSBSI Federal Scientific Vegetable Center), susceptible of aspermia tomato (Tomato aspermy cucumovirus) – AsTV. Seeds of strongly susceptible to AsTV varieties of salad Chameleon and Bouquet were subjected to thermotherapy at different temperatures (37°C, 38°C, 40°C) for a different time interval (1, 3, 5, 7, 10 days) in dry form and when moistened. Marked varietal specificity during germination of seeds after thermotherapy. Thus, the greatest number of seedlings in the emerald variety was obtained after 5 days of thermotherapy (10.0±0), while the Bouquet variety had the best results after 3 days of thermotherapy (9.3±1.2) with moisture. After thermotherapy of dry seeds by 40°C plant material of cultivar Emerald was planted on solid and liquid culture media. The conditions of step sterilization of lettuce seeds for introduction into the culture in vitro were chosen: washing in 96% ethanol, then in 50% aqueous solution of "Whiteness" with the addition of Twin-20, after in sterile distilled water. The nutrient medium for germination of lettuce seeds was used: Gamborg B5 (2% sucrose, 7.0 g/l agar), and the liquid nutrient medium was of that composition. The obtained seedlings were cutted and transferred to medium MS (2% sucrose, 0.1 mg/l ha and 1 mg/l BAP, 3.0 g/l phytogel). The formed shoots for rooting were transferred to the MS medium (2% sucrose, 3.0 g/l phytogel). In the future, lettuce plants will be adapted in vivo and tested for the presence of tomato aspermia virus in the planting material.

scholarly journals REGENERATION SYSTEM OF LETTUCE (LACTUCA SATIVA L.) THROUGH IN VITRO CULTURE

2019 ◽  
pp. 15-17
Author(s):  
O. V. Romanova ◽  
A. V. Soldatenko ◽  
V. S. Romanov
Keyword(s):  
Aqueous Solution ◽  
Lactuca Sativa ◽  
Leaf Explants ◽  
Distilled Water ◽  
Ms Medium ◽  
Regeneration System ◽  
Fluorescent Lamps ◽  
Lettuce Seeds ◽  
Petri Dishes

The article is devoted to the problem of obtaining regenerative plant varieties of lettuce (Lactuca sativa L.) Bouquet, Emerald, Chameleon (FSBSI Federal Scientific Vegetable Center). Salad culture is insensitive to in vitro conditions and requires the development of alternative regeneration conditions. Seeds were surface sterilized in 96% ethanol (30 s), then 15 minutes in 50% aqueous solution of "Whiteness" with the addition of Twin-20 (1 drop per 100 ml), washed three times in sterile distilled water. Then lettuce seeds were placed in Petri dishes on Gamborg B5 medium (2% sucrose, 3,0 g/l fitogeli). After 10 days, the obtained seedlings were cut, the leaf blades were cut into squares 1 cm in size and transferred to medium MS B5 (2% sucrose) with three variants of hormone concentrations: 0.1 mg/l NAA and 1 mg/l BAP, 1 mg/l NAA and 1 mg/l BAP, 1 mg/l NAA and 0.1 mg/l BAP. The formed shoots were rooted on MS medium (2% sucrose, 7,0 g/l agar). Cultivation was carried out on racks with fluorescent lamps at 25°C and a photoperiod of 14 hours, illumination of 2,5 thousand Lux. According to the results of studies, the concentration of hormones (0,1 mg/l NAA, 1 mg/l BAP) was selected, which allowed to obtain up to 10 shoots from one leaf explant. Moreover, the largest number of shoots was noted on leaf explants located closer to the veins of the sheet. The obtained data allowed to improve the system of regeneration of varieties of emerald lettuce, Bouquet and Chameleon.

Activity of glucose-6-phosphate dehydrogenase from lettuce seeds (Lactuca sativa)

1974 ◽  
Vol 52 (10) ◽  
pp. 2225-2227 ◽  
Author(s):  
Henry L. Speer
Keyword(s):  
Enzyme Activity ◽  
Lactuca Sativa ◽  
Red Light ◽  
Supernatant Fraction ◽  
Lettuce Seed ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Glucose 6 Phosphate Dehydrogenase

The effects of phosphate, arsenate, arsenite, and sulfate on lettuce seed (Lactuca sativa L.) glucose-6-phosphate dehydrogenase were investigated. All four substances inhibited the enzyme and inhibition was substrate dependent. The reaction was specific for glucose-6-phosphate and NADP. No effect of red or far-red light could be observed on enzyme activity. All enzyme activity was confined to the supernatant fraction.

scholarly journals Induction of Dormancy in Nondormant Seeds

1994 ◽  
Vol 119 (3) ◽  
pp. 408-413 ◽  
Author(s):  
Anwar A. Khan
Keyword(s):  
Abscisic Acid ◽  
Lycopersicon Esculentum ◽  
Gibberellic Acid ◽  
Lactuca Sativa ◽  
Daucus Carota ◽  
Apium Graveolens ◽  
Lettuce Seeds ◽  
Moist Chilling ◽  
Dormancy Induction ◽  
Ga Biosynthesis

A gibberellic acid (GA) biosynthesis inhibitor, tetcyclacis, induced dormancy in nondormant seeds of lettuce (Lactuca sativa L.), tomato (Lycopersicon esculentum Mill.), pepper (Capsicum annuum L.), carrot [Daucus carota var. sativus (Hoffn.)], onion (Allium cepa L.), celery (Apium graveolens L.), and impatiens (Impatiens novette), as most of the seeds failed to germinate after washing under conditions that permitted germination before dormancy induction. In lettuce seeds, tetcyclacis and paclobutrazol were more effective in inhibiting germination in light than in darkness. A 16- to 24-h soak treatment with tetcyclacis was sufficient to induce dormancy in nearly all seeds. Tetcyclacis failed to induce dormancy if applied after 6 h presoak in water. Dormancy induced by tetcyclacis was released by GA4+7 (a mixture of gibberellin A4 and A7), light, and moist-chilling treatments. When GA4+7 was applied with tetcyclacis, dormancy induction was prevented under both favorable, e.g., 25C, and unfavorable, e.g., 5C, or low water potential (Ψ), germination conditions. Unlike tetcyclacis, abscisic acid (ABA) failed to induce dormancy in lettuce seeds. Thermodormancy induction in lettuce seeds at 35C was prevented by fluridone. However, neither ABA nor tetcyclacis countered its effect. Dormancy was also induced in lettuce seeds by ancymidol, flurprimidol, or paclobutrazol. Dormancy induced by tetcyclacis in pepper, tomato, carrot, and onion seeds was released by GA4+7, but not by irradiation or moist-chilling. Chemical names used: 5-(4-chlorophenyl)-3, 4, 5, 9, 10-pentaazatetracyclo [5.4.102,6.08,11]-dodeca-3, 9-diene (tetcyclacis); 1-(4-chlorophenyl)-4, 4-dimethyl-2-(1H-1, 2, 4-triazole-1-yl)-3-pentanol (paclobutrazol); α-cyclopropyl-α-(4-methoxyphenyl)-5-pyrimidine methanol (ancymidol); α-(1-methyl)-α-[4-(trifluoromethoxy) phenyl]-5-pyrimidine-methanol (flurprimidol); 1-methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4 (1H)-pyridinone (fluridone).

scholarly journals Chemical Composition and Antifungal Effects of Vitex agnus-castus L. and Myrtus communis L. Plants

2016 ◽  
Vol 44 (2) ◽  
pp. 466-471 ◽  
Author(s):  
Melih YILAR ◽  
Yusuf BAYAN ◽  
Abdurrahman ONARAN
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Plant Pathogens ◽  
Petri Dish ◽  
Myrtus Communis ◽  
Mycelium Growth ◽  
Agnus Castus ◽  
Petri Dishes

The purpose of this study was to assess the effectiveness of essential plant oils from Vitex agnus-castus L. (VAC) and Myrtus communis L. against the plant pathogens, Fusarium oxysporum f. sp. radicis-lycopersici (Sacc.) W.C. Synder & H.N. Hans, Rhizoctonia solani J.G. Kühn., Sclerotinia sclerotiorum (Lib.) de Bary and Verticillium dahliae Kleb., and to determine the chemical composition of the compounds in these essential oils. GC/MS analysis was identified 25 different compounds in VAC essential oil, while the main compounds were determined as Eucalyptol (17.75%), β-Caryophyllene (13.21%) and Spathulenol (10.41%). On the other hand, the essential oil of M. communis, consisted of 16 different compounds which were Eucalyptol (49.15%), Myrtenol (19.49%) and α-Pinene (8.38%) being its main compounds. An assessment of antifungal activity was performed under in vitro conditions. Plant pathogens were inoculated onto Petri dishes (60 mm) containing PDA medium (10 mL/Petri-1), and plant essential oils were applied at concentrations of 0.5, 1, 1.5, 2, 5 and 10 (μL/Petri-1) into the 5 mm diameter wells opened on the Petri dish surface. After that, the Petri dishes incubated at 22±2 °C. The results of this study, the essential oil of M. communis, at a dose of 10 μL/ Petri, inhibited the 100% mycelium growth of V. dahliae, S. sclerotiorum and R. solani. The highest dose of VAC essential oil was also 100% inhibited V. dahliae and S. sclerotiorum. The LC50 and LC90 values of M. communis and VAC essential oil calculated for V. dahliae, FORL, S. sclerotiorum and R. solani. This plant extracts were shown by in vitro conditions to be potential antifungal agents.

Sperm Performance Better on Diamond than on Polystyrene

2013 ◽  
Vol 1511 ◽  
Author(s):  
Andrei P. Sommer ◽  
Dan Zhu ◽  
Friedrich Gagsteiger ◽  
Hans-Jörg Fecht
Keyword(s):  
Embryonal Carcinoma ◽  
Petri Dish ◽  
Carcinoma Cells ◽  
Oxygen Species ◽  
Embryonal Carcinoma Cells ◽  
Vitro Fertilization ◽  
Polystyrene Petri Dish ◽  
Petri Dishes ◽  
New Generation

ABSTRACTRecently we postulated that polystyrene Petri dishes become soft when in contact with an aqueous milieu. Specifically, we assumed that the effect is restricted to a superficial nanolayer, a condition presumably favoring the establishment of a stable nanolayer of reactive oxygen species (ROS) at the liquid/solid-interface. Cells are known to be hypersensitive to ROS. Previously we used P19 mouse embryonal carcinoma cells and systematically analyzed their capability to climb different substrates placed vertically into a Petri dish. The worst and best performance was found on polystyrene (Petri dish material) and nanocrystalline diamond, respectively. Polystyrene Petri dishes are today standard in laboratories conducting in vitro fertilization (IVF). Here we proceed and extend the investigation to human spermatozoa and show that their performance (vitality) on polystyrene Petri dishes is low compared to that on diamond Petri dishes. This work may propel further research and inspire the development of a new generation of cell-friendly Petri dishes.

scholarly journals Effect of landfill leachate on germination of lettuce seeds (Lactuca sativa L.)

2017 ◽  
Vol 21 ◽  
pp. 67
Author(s):  
Heider Alves Franco ◽  
Monica Regina da Costa Marques ◽  
Clarisse Ferreira Braga ◽  
Allana De Souza Izidorio ◽  
Sérgio Thode Filho
Keyword(s):  
Lactuca Sativa ◽  
Landfill Leachate ◽  
Lettuce Seeds ◽  
Lactuca Sativa L
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