Antioxidant, antibacterial activities and gc-ms analysis of methanol extract of buds of Hypericum hookerianum wight and arnott

2020 ◽  
Vol 13 (8) ◽  
pp. 3709
Author(s):  
S Brintha ◽  
C Sivaraj ◽  
K Saraswathi ◽  
P Arumugam ◽  
L Jeyanthi Rebecca
2019 ◽  
Vol 11 (5) ◽  
pp. 1055-1063
Author(s):  
Choudhary Choudhary D ◽  
Shekhawat Shekhawat JK ◽  
Kataria Kataria V

Author(s):  
PAVITHRA S ◽  
SEKAR T

Objective: In the present study is investigated of phytochemicals and antioxidant activities of the leaf extracts from Meliosma simplicifolia (L.). Methods: The seaweed sample was subjected to extraction with solvents of different polarities (hot water, methanol, acetone, chloroform, and petroleum ether) and screened for phytochemicals according to standard methods. The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, and superoxide radical (O2•) scavenging activity. The ICPMS and GC-MS analysis of the methanol leaf extract of M. simplicifolia was revealed the presence of antibacterial. Results: Phytochemical analysis revealed the presence of Preliminary metabolites Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (40.71±0.94 GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia leaf for as followed the studies. In leaf the highest DPPH scavenging activity (132.3 μg/ml), ICP-MS analysis of the leaf extract showed the presence minerals such as Mg, Fe, Cu, Na, and Zn in excess. The leaf extract of the plant was also tested for its antibacterial activity and results confirmed that it has potential activity. Conclusion: The preliminary studies in the methanol extract of the leaf of M. simplicifolia are suggestive of the antibacterial potentials of the methanol extract of leaves of M. simplicifolia.


2011 ◽  
Vol 11 ◽  
pp. 2237-2242 ◽  
Author(s):  
S. C. Sati ◽  
Savita Joshi

The antibacterial activity of methanol, ethanol, chloroform, and hexane extracts of the leaves of Himalayan gymnospermous plantGinkgo bilobaL. was assessed against five animal and plant pathogenic strains (Agrobacterium tumefaciens, Bacillus subtilis, Escherichia coli, Erwinia chrysanthemi, and Xanthomonas phaseoli) employing disc-diffusion and broth-dilution assays. The methanol extract showed the highest activity (zone of inhibition of 15–21 mm) followed by ethanol (14–19 mm), chloroform (15–20 mm), and hexane (14–19 mm) extracts at 250 μg/mL. A minimum inhibitory concentration (MIC) of 7.8 μg/mL was found for the methanol extract against most of the pathogens tested.


Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4271
Author(s):  
Asma Alshamari ◽  
Mahmoud Al-Qudah ◽  
Fedaa Hamadeh ◽  
Lo’ay Al-Momani ◽  
Sultan Abu-Orabi

A series of derivatives of trans-3-(2,4,6-trimethoxyphenyl)4,5-dihydroisoxazolo-4,5-bis[carbonyl-(4′phenyl)thiosemicarbazide (9) and of trans-3-(2,4,6-trimethoxyphenyl)-4,5-dihydro isoxazolo-4,5-bis(aroylcarbohydrazide) (10a–c) were synthesized from trans-3-(2,4,6-trimethoxyphenyl)-4,5-dihydro-4,5-bis(hydrazenocarbonyl)isoxazole (8). The structures of the compounds were elucidated by both elemental and spectral (IR, NMR, and MS) analysis. Compound 9 shows activity against some bacterial species. No antibacterial activities were observed for compounds 10a–c. The antioxidant activity of the new compounds has been screened. Compound 9 showed higher antioxidant activity using the DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2’-azino–bis(3-ethylbenzoline-6-sulfonic acid) diammonium salt methods.


Author(s):  
Igor K. Voukeng ◽  
Blaise K. Nganou ◽  
Louis P. Sandjo ◽  
Ilhami Celik ◽  
Veronique P. Beng ◽  
...  

2016 ◽  
Vol 11 (4) ◽  
pp. 920 ◽  
Author(s):  
Vineetha M. Shrikanth ◽  
Bhavya Janardhan ◽  
Sunil S. More

<p class="Abstract"><em>Canthium </em>parviflorum is used in the traditional therapy to treat snakebite victims. In the present study, partially purified methanol root extract of <em>C. </em>parviflorum was used for the neutralization of Eachis carinatus and <em>Naja naja</em> venom. The extract inhibited <em>in vitro</em> phosphomonoesterase, phosphodiesterase, acetylcholinesterase, hyaluronidase, protease, phospholipase A<sub>2 </sub>and 5’nucleotidase activities of both venoms. One of the promising band exhibited neutralization of all the<em> in vitro</em> enzyme activities and was further subjected to GC-MS analysis which revealed the presence of eight active phytocompounds. These phytochemicals might be responsible for <em>in vitro</em> enzyme neutralization. Methanol extract possesses potent active phytochemicals against the site specific toxins and hydrolytic enzymes analyzed.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract"><a href="https://youtube.com/v/oSLdFcfaJxU">Phospholipase A2 assay</a>: 1 min 36 sec</p>


2019 ◽  
Vol 9 (2) ◽  
pp. 28-30
Author(s):  
Kalpana Garg ◽  
Birendra Shrivastava ◽  
Anurag Bhargava

Introduction: To identify the various phytoconstituents present in the plant Sphaeranthus indicus by using gas chromatography and mass spectrometry. Meth­ods: The fruits of Sphaeranthus indicus were extracted with Different solvents of increasing polarity. The methanol and ethylacetate extract were subjected to GCMS analysis to detect the phytoconstituents. Results: Totally 26 compounds were identified. Among these 13 constituents in methanol extract and 13 constituents in ethylacetate extract were identified during the GC-MS analysis. Stigmasterol and lupeol which were identified in the plant is considered to have antiarthritic properties. Keywords: Sphaeranthus indicus, Gas chromatography, Mass spectrometry.


2019 ◽  
Vol 5 (3) ◽  
pp. 52
Author(s):  
Ahmed Yacouba Coulibaly ◽  
Pierre Alexandre Eric Djifaby Sombié ◽  
Rokiah Hashim ◽  
Shaida Fariza Sulaiman ◽  
Othman Sulaiman ◽  
...  

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