scholarly journals ALLELIC COMPOSITION OF HMW-GLUTENIN PROTEIN AND THEIR RELATIONSHIP WITH QUALITY OF WHEAT

AGROFOR ◽  
2018 ◽  
Vol 3 (2) ◽  
Author(s):  
Desimir KNEŽEVIĆ ◽  
Aleksandra Novoselskaya DRAGOVIČ ◽  
Aleksandr KUDRYAVCEV ◽  
Danijela KONDIĆ ◽  
Gordana BRANKOVIĆ ◽  
...  

High molecular weight glutenin subunits (HMW-GS) proteins deposited inendosperm of wheat seed which have significant impact on bread quality. TheHMW-GS encoded by genes located at the long arm of chromosomes 1A, 1B, 1D.The aim of this work was study allele polymorphysms at Glu-A1, Glu-B1 and Glu-D1 locus and loaf volume, grain protein content, sedimentation volume of eightwheat genotypes (G-3130, G-35183, G-3501, G3512, G-3574, G-3027, G-3075, G-3097) harvested in two years with various weather condition. For each genotypes,flour used for extraction of glutenin which separated by method of electrophoresison SDS gel (11.8%). Electrophoregrams used for determining Glu-1 alleles.Technological quality parameters analyzed by standard laboratory methods. Thethree alleles alleles (a, b, c) at the Glu-A1, three alleles (b, c, d) at the Glu-B1 and 2alleles (a, d) at the Glu-D1 were identified. The highest protein sedimentationvolume had wheat genotype G-3075 in the both years (54.0ml; 58.0ml) while thelowest sedimentation volume had G-3512 (34.0ml; 36.0ml). Grain protein content(GPC) was the highest in G-3075 in both years (14.20%; 15.40%) while the lowestGPC had G-3097 (11.60%) in first and G-3512 (12.60%) in the second year. Loafvolume was the highest in G-3075 in both year (520ml; 540ml) while the lowestwas in G-3512 (400ml) in both years of experiment. The estimated quality traitsvaried depending on genotype and year. The better quality, in average, had thewheat genotypes which carried Glu-D1d allele.

2021 ◽  
Author(s):  
Pushpendra K. Gupta ◽  
Harindra S. Balyan ◽  
Parveen Chhuneja ◽  
Jai P. Jaiswal ◽  
Shubhada Tamhankar ◽  
...  

Abstract Improvement of grain protein content (GPC), loaf volume and resistance to rusts was achieved in 11 Indian wheat cultivars that are widely grown in four different agro-climatic zones of India. This involved use of marker-assisted backcrossing (MABC) for introgression and pyramiding of the following genes: (i) the high GPC gene Gpc-B1; (ii) HMW glutenin subunits 5 + 10 at Glu-D1 loci, and (iii) rust resistance genes, Yr36, Yr15, Lr24 and Sr24. GPC was improved by 0.8–3.3%, although high GPC was generally associated with yield penalty. Further selection among high GPC lines, allowed development of progenies with higher GPC associated with improvement in 1000-grain weight and grain yield in the following four cultivars: NI5439, UP2338, UP2382 and HUW468. The high GPC progenies (derived from NI5439) were also improved for grain quality using HMW glutenin subunits 5 + 10 at Glu-D1 loci. Similarly, progenies combining high GPC and rust resistance were developed in the backgrounds of following five cultivars: Lok1, HD2967, PBW550, PBW621 and DBW1. The improved pre-bred lines developed during the present study should prove useful for development of cultivars with improved nutritional quality associated with rust resistance in future wheat breeding programmes.


1990 ◽  
Vol 70 (3) ◽  
pp. 629-637 ◽  
Author(s):  
CHARLES F. McGUIRE ◽  
LARRY G. BLACKWOOD

The United States Department of Agriculture (USDA) grading standards for wheat places hard red spring and hard red winter (Triticum aestivum L. em. Thell) wheat into separate classes. One important criterion for this designation is kernel type. Because of genotypes being released by plant breeders in recent years, distinction between these two classes is difficult for grain graders. As a consequence some people in the grain industry favor placing both of these wheat types into one class. One hazard of this action is that end use properties of these two wheats, according to some industrial firms, is class dependent. We studied quality characteristics of five hard red spring and seven hard red winter wheat cultivars grown at the same three Montana locations in 5 different years to evaluate this concept. Analysis of variance indicated quality differences between classes for all traits except flour yields, which were similar for the two classes. Flour ash content, farinograph absorption, peak time, stability time, valorimeter, grain protein content, bake absorption, mix time, and loaf volume were all significantly higher for spring than winter wheats. These values were still higher for spring than winter wheats except for test weight when wheat protein content was the co-variate. Both statistical treatments show that hard red spring wheat flour has higher water absorption percent, longer dough mixing requirements, longer dough stability times, and higher loaf volumes than hard red winter wheat flour.Key words: Bread wheat quality, loaf volume, grain protein content, protein quality


2021 ◽  
Author(s):  
Mekonnen Gebeyaw

The greatest use of barley for malting purpose mostly for brewing industry. The quality of malt depends upon various grain parameters as kernel shape, size, boldness, grain protein content etc., which affects the malt quality that is malt yield, friability, homogeneity.The availability of barley for malting is not a problem, but whatever barley is available it is very poor interims of quality and not meeting the minimum standards of malting quality. So, that identification of malt barely varieties with different grain and malt parameters, which are desired for better malt production and quality improvement, needed for various products is very essential. Potential areas that boost the production, pertinent agronomic practice studies and strengthening micro malting laboratory and expert capacity are recommended to overcome the limitations of malt barley production and malt quality improvement.


Author(s):  
А.N. FILATOV ◽  
◽  
V.N. MAZUROV ◽  
V.K. KHRAMOY ◽  
E.R. ARLANTSEVA

Providing the brewing industry with locally produced raw materials is important both from an economic point of view and in terms of import substitution. Breeders create brewing barley varieties with a reduced protein content, however, in production they often use universal varieties that combine high yields with an average protein content of 11–12%. For such varieties, it is necessary to develop technological methods for obtaining a crop with specified properties, depending on the purposes of using the products. The quality indicators of barley grain and the efficiency of its production are significantly influenced by mineral fertilizers and the minimization of soil cultivation. In this regard, the authors studied the effect of minimal tillage on the yield and grain quality of spring barley of the Vladimir variety at two levels of mineral nutrition (without fertilizers 26 and with full supply oif mineral fertilizer – N35P40K135) in a two-factor field experiment carried out in 2018–2019 on the medium-loamy light gray forest soil. The experiment helped determine the yield and structure of the crop, the content of protein, starch, and grain hoodness (hull content). It was found that the lack of precipitation during the barley tillering – heading period leads to a decrease in grain yield by 40.0–54.8% as compared with the conditions of increased moisturizing. At the same time, there was an increase in the grain protein content by 1.4–1.7% and the grain filminess by 0.5–0.9%. The use of moderate rates of complete mineral fertilizer (N35P40K85) led to an increase in the barley yield by an average of 13.2% and the grain protein content by an average of 0.2%. On the other hand, it led to a decrease in the grain filminess by 0.2% and starch content by 0.4–0.6%. The positive effect of nitrogen fertilizers on the increase in protein content in barley grain was higher under minimal tillage. Under minimal tillage, regardless of the level of moisture supply and mineral nutrition, there was a tendency towards a decrease in the barley yield by an average of 3.9%, mainly due to a decrease in the density of the productive stalk. There was also a tendency towards a decrease in the protein content in the barley grain – by 0.3% – when no complete mineral fertilizer was used.


2019 ◽  
Vol 40 (6) ◽  
pp. 2571 ◽  
Author(s):  
Raphael Rossi Silva ◽  
Claudemir Zucareli ◽  
Inês Cristina de Batista Fonseca ◽  
Diego Gazola ◽  
Carlos Roberto Riede

The quality of wheat is a pre-eminent factor in the criteria for commercialization of this cereal, and several parameters contribute to the evaluation of wheat quality. The objective of this study was to verify the effects of nitrogen fertilization on the cover and the environment for wheat cultivars and the effects on the industrial quality of the grain. Furthermore, we sought to identify correlations between the parameters of industrial quality. Experiments were conducted in Londrina for 2 years, in two distinct environments (A1 and A2). The experimental design was a randomized complete block design with four replications. The cultivars IPR Catuara TM, BRS Gaivota, Quartzo, and CD 120 were evaluated with different forms of nitrogen management: N1, without N in coverage; N2, 60 kg ha-1 of N in the form of urea at the beginning of tillering; N3, 80 kg ha-1 of N in urea formulation (60 kg ha-1 in tillering and 20 kg ha-1 in booting); N4, 100 kg ha-1 of N in the form of urea (60 kg ha-1 in tillering and 40 kg ha-1 in booting); N5, 80 kg ha-1 of N (60 kg ha-1 of N in the form of urea at the beginning of tillering, and 20 kg ha-1 of N in the form of ammonium sulfate in booting); N6, 100 kg ha-1 of N (60 kg ha-1 of N in the form of urea at the beginning of tillering, and 40 kg ha-1 of N in the form of ammonium sulfate in booting). Further, we evaluated grain yield, protein content, sedimentation volume with sodium dodecyl sulfate, and parameters of alveograph and farinograph. Nitrogen fertilization did not influence industrial quality parameters. The stability of the mass was influenced by the isolated effects of cultivars and the environment. The interaction of cultivars and environments influenced the parameters of alveograph and water absorption of the mass. The higher the protein content, the lower the stability of the mass, independent of the cultivar.


2021 ◽  
Vol 29 ◽  
pp. 87-91
Author(s):  
N. A. Kozub ◽  
I.O. Sozinov ◽  
H.Ya. Bidnyk ◽  
N.O. Demianova ◽  
O.I. Sozinova ◽  
...  

Aim. The aim of this study was to analyze grain quality indices in winter common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis. marked by storage protein loci. Methods. Acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins were performed to identify introgressions. Grain quality indices (SDS sedimentation volume and grain protein content) were analyzed in lines with introgressed chromosome 1U or its arm 1UL, as well as in the cultivars Panna and Bezostaya 1. Results. SDS-sedimentation volume in the cultivars and lines depended on year’s conditions. The studying of the lines during two years has demonstrated that the presence of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis was associated with a high volume of SDS-sedimentation SDS30 (higher than that in the cultivar Bezostaya 1). The introgressive lines show high grain protein content. Conclusions. The effect of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis on SDS-sedimentation value is similar to that of the high-quality allele Glu-B1al. The lines with the introgressed allele at Glu-U1 from Ae. biuncialis are valuable initial material for breeding for quality.Keywords: Triticum aestivum, Aegilops biuncialis, high molecular weight glutenin subunits, SDS-sedimentation, protein content.


2021 ◽  
Vol 31 (3) ◽  
pp. 105-110
Author(s):  
Batbold Serchin ◽  
Ganbaatar Bodisad ◽  
Urtnasan Ganbaatar

The study was provided in IPAS during 2016-2019. The objective of this study was to determine the grain quality characteristics of spring wheat varieties based on bread making quality related parameters. Results of study shown early maturing varieties Darkhan-160, Darkhan-172 have 0.5-2.1 cn/ha more yield than Darkhan-131 and middle maturing varieties Darkhan-74, Altaiskaya-325, Altaiskaya-70 have 0.3-2.5 cn/ha more yield than Darkhan-131. Comparing grain protein content of favorable and drought condition early maturing varieties were reduced protein content by 2.0%, middle maturing – 1.8% and late maturing – 1.3%. By ranking grain quality parameters wheat varieties Darkhan-160, Buryatskaya ostistaya, Arvin, Darkhan-144 and  Darkhan-181have a good quality. Буудайн сортуудын ургац ба чанарыг судалсан дүн Монгол оронд нутагшсан, үйлдвэрлэлд зонхилон тариалагдаж буй буудайн нийт 16 сортуудыг ургац ба чанарын үзүүлэлтүүдээр харьцуулан судалж арвин ургацтай, чанар сайтай сортыг илрүүлэх зорилгоор судалгааг 2016-2019 онд УГТХ-ийн Үр тарианы селекцийн туршлагын талбайд явуулсан. Судалгааны дүнгээр эртийн болцтой Дархан-160, Дархан-172 сортууд (ХХБЗ05=0.5 ц/га) жишиг Дархан-131 сортыг, Дархан-160, Дархан-172 сортууд дунд эртийн болцтой Алтайская-325, Алтайская-70 сортууд (ХХБЗ05=1.2 ц/га) жишиг Дархан-34 сортыг бодитой давсан нэмүү  ургац тус тус  бүрдүүллээ.  Харин дунд-оройн болцтой Дархан-181 сорт (ХХБЗ05=1.5 ц/га) жишиг Дархан-144 сортоос 1.5 ц/га бага ургацтай байсан боловч энэ нь алдааны түвшинд байв. Чийгтэй жилийн уургийн агуулалтыг гантай жилийнхтэй харьцуулахад буудайн сортуудын уургийн агуулалтын бууралт нь эртийн болцтой бүлэгт 2.0%, дунд эртийн болцтой бүлэгт 1.8%, дунд оройн болцтой бүлэгт 1.3% байлаа.  Уургийн агуулалт эртийн болцтой бүлгээс Дархан-160 (12.6%), дунд эртийн болцтой бүлгээс Дархан-74 (5.9%), дунд-оройн болцтой бүлгээс Дархан-144 (7.2%) зэрэг сортуудад хамгийн бага буурсан. Буудайн сортуудын гурил, талхны чанарын нэгдсэн дүнд ранкын үнэлгээ өгөхөд Дархан-160, Бурятская остистая, Дархан-144, Арвин, Дархан-181 сортууд шалгарч байна. Эдгээр сортууд нь арвин ургац бүрдүүлдэг, хүнсний буудайн улсын стандарт шаардлагын I, II ангиллыг хангадаг, гурил, талхны чанар сайтай учир зах зээлд өрсөлдөх чадвартай, хүнсний аюулгүй байдлыг  хангах чухал нөөц болно.  Түлхүүр үг: ургац, уураг, цавуулаг, гурил, талх, ранк


2012 ◽  
Vol 40 (4) ◽  
pp. 532-541 ◽  
Author(s):  
V. Mladenov ◽  
B. Banjac ◽  
A. Krishna ◽  
M. Milošević

Sign in / Sign up

Export Citation Format

Share Document