scholarly journals Estimation of antioxidative potential of thyme (Thymus alpestris L.)

2017 ◽  
Vol 1 (9) ◽  
Author(s):  
Ljubica Vasiljević ◽  
Mirjana Beribaka ◽  
Jelena Vulinović ◽  
Slađana Petronić
Keyword(s):  
Phenolic Compounds ◽  
Plant Species ◽  
Ethanol Extract ◽  
Antioxidant Potential ◽  
Soxhlet Method ◽  
Polyphenolic Acids

Family Lamiaceae (labia) includes a large number of cosmopolitan speciesincluding Thymus alpestris (Thyme) characterized by the presence of essential oilsand phenolic compounds. It represents a perennial semi-shrubby plant. Theantioxidant potential of thyme is due to the presence of polyphenolic acids andflavonoids. The topic of this paper is the extraction of thyme (Thymus alpestris)with 40% ethanol and 50% ethanol, using Soxhlet method (circulatoryextraction), after which, the content of phenolics and flavonoids was determinedin order to confirm the antioxidant potential of this plant species. The resultsshow that 40% ethanol extract has the highest content of phenolics andflavonoids and the highest antioxidant potential, resultingly.

scholarly journals Bioactivity and Toxicity of Senna cana and Senna pendula Extracts

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
J. A. Monteiro ◽  
J. M. Ferreira Júnior ◽  
I. R. Oliveira ◽  
F. L. A. Batista ◽  
C. C. C. Pinto ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Polyphenolic Compounds ◽  
Hexane Extract ◽  
Antioxidant Potential ◽  
Therapeutic Uses ◽  
Inhibitory Activities ◽  
Ethanol Extracts

This work investigated the content of total polyphenolic compounds and flavonoids as well as their toxicity and larvicidal and acetylcholinesterase inhibitory activities. The antioxidant activities of two medicinal Senna species extracts (Senna cana and Senna pendula) were also investigated. The ethanol extract of the leaves of S. cana and the ethanol extract of the branches of S. pendula presented the best performance in the DPPH/FRAP and ABTS/ORAC assays, respectively. For the inhibition of acetylcholinesterase, the hexane extract of the flowers of S. pendula presented the lowest IC50 value among the ethanol extracts of the leaves of S. cana and showed the best performance in some assays. The hexane extract of the leaves of S. pendula and the hexane extract of the branches of S. cana were moderate to Artemia salina Leach. In the quantification of phenols and flavonoids, the ethanol extract of the leaves of S. cana presented the best results. The ethanol extracts of the leaves of S. cana were found to be rich in antioxidants, phenolic compounds, and flavonoids. These results indicate the antioxidant potential of the extracts of Senna species and can be responsible for some of the therapeutic uses of these plants.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Etanol 70% Bunga Telang (Clitoria ternatea L) dari Daerah Sleman dengan Metode DPPH

2020 ◽  
Vol 17 (1) ◽  
pp. 70-76
Author(s):  
Disa Andriani ◽  
Lusia Murtisiwi
Keyword(s):  
Free Radicals ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Antioxidant Potential ◽  
Clitoria Ternatea ◽  
Flower Extract ◽  
Ic50 Value

Telang flower (Clitoria ternatea L.) contains phenolic compounds which can act as antioxidants by donating hydrogen so as to stabilize the lack of electrons in free radicals. This study aims to determine the antioxidant potential of telang flower ethanol extract by looking at the IC50 value. Telang flowers were extracted by maceration method using 70% ethanol solvent. The potential for antioxidants was determined by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method with a comparison of vitamin C which had been shown to have the potential of free radicals which were very potent. The results showed the IC50 value of telang flower ethanol extract was 41.36 ± 1.191 μg / mL, based on that value the telang flower extract from the Sleman area was in the very potent category so that it could be developed as a source of antioxidants from natural ingredients.

Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits

2020 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Nadezhda Petkova ◽  
Manol Ognyanov ◽  
Blaga Inyutin ◽  
Petar Zhelev ◽  
Panteley Denev
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Frap Assay ◽  
Phytochemical Composition ◽  
Malus Baccata

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

scholarly journals High-Throughput Screening and Characterization of Phenolic Compounds in Stone Fruits Waste by LC-ESI-QTOF-MS/MS and Their Potential Antioxidant Activities

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 234 ◽  
Author(s):  
Yili Hong ◽  
Zening Wang ◽  
Colin J. Barrow ◽  
Frank R. Dunshea ◽  
Hafiz A. R. Suleria
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Prunus Persica ◽  
Phenolic Content ◽  
Antioxidant Potential ◽  
Stone Fruit ◽  
Total Phenolic ◽  
Stone Fruits ◽  
Antioxidant Power ◽  
Pharmaceutical Industries

Stone fruits, including peach (Prunus persica L.), nectarine (Prunus nucipersica L.), plum (Prunus domestica L.) and apricot (Prunus armeniaca L.) are common commercial fruits in the market. However, a huge amount of stone fruits waste is produced throughout the food supply chain during picking, handling, processing, packaging, storage, transportation, retailing and final consumption. These stone fruits waste contain high phenolic content which are the main contributors to the antioxidant potential and associated health benefits. The antioxidant results showed that plum waste contained higher concentrations of total phenolic content (TPC) (0.94 ± 0.07 mg gallic acid equivalents (GAE)/g) and total flavonoid content (TFC) (0.34 ± 0.01 mg quercetin equivalents (QE)/g), while apricot waste contained a higher concentration of total tannin content (TTC) (0.19 ± 0.03 mg catechin equivalents (CE)/g) and DPPH activity (1.47 ± 0.12 mg ascorbic acid equivalents (AAE)/g). However, nectarine waste had higher antioxidant capacity in ferric reducing-antioxidant power (FRAP) (0.98 ± 0.02 mg AAE/g) and total antioxidant capacity (TAC) (0.91 ± 0.09 mg AAE/g) assays, while peach waste showed higher antioxidant capacity in 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay (0.43 ± 0.09 mg AAE/g) as compared to other stone fruits waste. Qualitative and quantitative phenolic analysis of Australian grown stone fruits waste were conducted by liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS) and HPLC-photodiode array detection (PDA). The LC-ESI-QTOF-MS/MS result indicates that 59 phenolic compounds were tentatively characterized in peach (33 compounds), nectarine (28), plum (38) and apricot (23). The HPLC-PDA indicated that p-hydroxybenzoic acid (18.64 ± 1.30 mg/g) was detected to be the most dominant phenolic acid and quercetin (19.68 ± 1.38 mg/g) was the most significant flavonoid in stone fruits waste. Hence, it could be concluded that stone fruit waste contains various phenolic compounds and have antioxidant potential. The results could support the applications of these stone fruit wastes in other food, feed, nutraceutical and pharmaceutical industries.

scholarly journals Screening of Phenolic Compounds in Australian Grown Berries by LC-ESI-QTOF-MS/MS and Determination of Their Antioxidant Potential

Antioxidants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 26
Author(s):  
Vigasini Subbiah ◽  
Biming Zhong ◽  
Malik A. Nawaz ◽  
Colin J. Barrow ◽  
Frank R. Dunshea ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acid ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Vaccinium Corymbosum ◽  
Rubus Idaeus ◽  
Antioxidant Potential ◽  
Fresh Frozen ◽  
Flavonoid Quercetin

Berries are grown worldwide with the most consumed berries being blackberries (Rubus spp.), blueberries (Vaccinium corymbosum), red raspberries (Rubus idaeus) and strawberries (Fragaria spp.). Berries are either consumed fresh, frozen, or processed into wines, juices, and jams. In recent times, researchers have focused their attention on berries due to their abundance in phenolic compounds. The current study aimed to evaluate the phenolic content and their antioxidant potential followed by characterization and quantification using LC-ESI-QTOF-MS/MS and HPLC-PDA. Blueberries were highest in TPC (2.93 ± 0.07 mg GAE/gf.w.) and TFC (70.31 ± 1.21 µg QE/gf.w.), whereas the blackberries had the highest content in TTC (11.32 ± 0.13 mg CE/gf.w.). Blueberries had the highest radical scavenging capacities for the DPPH (1.69 ± 0.09 mg AAE/gf.w.), FRAP (367.43 ± 3.09 µg AAE/gf.w.), TAC (1.47 ± 0.20 mg AAE/gf.w.) and ABTS was highest in strawberries (3.67 ± 0.14 mg AAE/gf.w.). LC-ESI-QTOF-MS/MS study identified a total of 65 compounds including 42 compounds in strawberries, 30 compounds in raspberries, 28 compounds in blueberries and 21 compounds in blackberries. The HPLC-PDA quantification observed phenolic acid (p-hydroxybenzoic) and flavonoid (quercetin-3-rhamnoside) higher in blueberries compared to other berries. Our study showed the presence of phenolic acids and provides information to be utilized as an ingredient in food, pharmaceutical and nutraceutical industries.

scholarly journals Profile, antioxidant potential, and applicability of phenolic compounds extracted from Spirulina platensis

2015 ◽  
Vol 14 (41) ◽  
pp. 2903-2909 ◽  
Author(s):  
Denardi de Souza Taiana ◽  
Prietto Luciana ◽  
Moraes de Souza Michele ◽  
Badiale Furlong Eliana
Keyword(s):  
Phenolic Compounds ◽  
Spirulina Platensis ◽  
Antioxidant Potential

scholarly journals Sephadex® LH-20, Isolation, and Purification of Flavonoids from Plant Species: A Comprehensive Review

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4146
Author(s):  
Javad Mottaghipisheh ◽  
Marcello Iriti
Keyword(s):  
Phenolic Compounds ◽  
Secondary Metabolites ◽  
Plant Species ◽  
Health Benefits ◽  
Significant Information ◽  
Comprehensive Review ◽  
Isolation And Purification ◽  
Phytochemical Investigation ◽  
Plant Families ◽  
Pure Methanol

Flavonoids are considered one of the most diverse phenolic compounds possessing several valuable health benefits. The present study aimed at gathering all correlated reports, in which Sephadex® LH-20 (SLH) has been utilized as the final step to isolate or purify of flavonoid derivatives among all plant families. Overall, 189 flavonoids have been documented, while the majority were identified from the Asteraceae, Moraceae, and Poaceae families. Application of SLH has led to isolate 79 flavonols, 63 flavones, and 18 flavanones. Homoisoflavanoids, and proanthocyanidins have only been isolated from the Asparagaceae and Lauraceae families, respectively, while the Asteraceae was the richest in flavones possessing 22 derivatives. Six flavones, four flavonols, three homoisoflavonoids, one flavanone, a flavanol, and an isoflavanol have been isolated as the new secondary metabolites. This technique has been able to isolate quercetin from 19 plant species, along with its 31 derivatives. Pure methanol and in combination with water, chloroform, and dichloromethane have generally been used as eluents. This comprehensive review provides significant information regarding to remarkably use of SLH in isolation and purification of flavonoids from all the plant families; thus, it might be considered an appreciable guideline for further phytochemical investigation of these compounds.

scholarly journals Antioxidant and fatty acid profile of gabiroba seed (Campomanesisa Xanthocarpa Berg)

2012 ◽  
Vol 32 (2) ◽  
pp. 234-238 ◽  
Author(s):  
Marli da Silva Santos ◽  
Obdulio Gomes Miguel ◽  
Carmen Lúcia Oliveira Petkowicz ◽  
Lys Mary Bileski Cândido
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Unsaturated Fatty Acids ◽  
Ethanol Extract ◽  
Solvent Polarity ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Total Phenolic Compounds

This study aimed to evaluate the antioxidant potential and fatty acid profile of gabiroba (Campomanesia xanthocarpa Berg) seeds. In order to obtain the extract, the seeds were dried, crushed, and subjected to sequential extraction by maceration and percolation in a modified soxhlet extractor using solvent polarity gradient composed of hexane, chloroform, ethyl acetate, and alcohol, respectively. The extraction time was six hours. The ethanol extract showed the highest antioxidant potential, given by the EC50 value and the amount of total phenolic compounds. High amounts of unsaturated fatty acids were found in the oil studied, especially the oleic acid.

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