scholarly journals RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae)

2018 ◽  
Author(s):  
Ruixue Ran ◽  
Tianyu Li ◽  
Xinxin Liu ◽  
Hejia Ni ◽  
Wenbin Li ◽  
...  
Keyword(s):  
Rna Interference ◽  
Larval Mortality ◽  
Crop Protection ◽  
Abnormal Development ◽  
Signalling Molecules ◽  
Rnai Technology ◽  
Pod Borer ◽  
Soybean Pest ◽  
Protection Strategies ◽  
Immune Related Genes

RNA interference (RNAi) technology may be useful for developing new crop protection strategies against the soybean pod borer (SPB; Leguminivora glycinivorella), which is a critical soybean pest in northeastern Asia. Immune-related genes have been recently identified as potential RNAi targets for controlling insects. However, little is known about these genes or mechanisms underlying their expression in the SPB. In this study, we completed a transcriptome-wide analysis of SPB immune-related genes. We identified 41 genes associated with SPB microbial recognition proteins, immune-related effectors or signalling molecules in immune response pathways (e.g., Toll and immune deficiency pathways). Eleven of these genes were selected for a double-stranded RNA artificial feeding assay. The down-regulated expression levels of LgToll-5-1a and LgPGRP-LB2a resulted in relatively high larval mortality rates and abnormal development. Our data represent a comprehensive genetic resource for immune-related SPB genes, and may contribute to the elucidation of the mechanism regulating innate immunity in Lepidoptera species. Furthermore, two immune-related SPB genes were identified as potential RNAi targets, which may be used in the development of RNAi-mediated SPB control methods.

scholarly journals RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae)

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4931 ◽  
Author(s):  
Ruixue Ran ◽  
Tianyu Li ◽  
Xinxin Liu ◽  
Hejia Ni ◽  
Wenbin Li ◽  
...  
Keyword(s):  
Rna Interference ◽  
Larval Mortality ◽  
Crop Protection ◽  
Abnormal Development ◽  
Signalling Molecules ◽  
Rnai Technology ◽  
Pod Borer ◽  
Soybean Pest ◽  
Protection Strategies ◽  
Immune Related Genes

RNA interference (RNAi) technology may be useful for developing new crop protection strategies against the soybean pod borer (SPB; Leguminivora glycinivorella), which is a critical soybean pest in northeastern Asia. Immune-related genes have been recently identified as potential RNAi targets for controlling insects. However, little is known about these genes or mechanisms underlying their expression in the SPB. In this study, we completed a transcriptome-wide analysis of SPB immune-related genes. We identified 41 genes associated with SPB microbial recognition proteins, immune-related effectors or signalling molecules in immune response pathways (e.g., Toll and immune deficiency pathways). Eleven of these genes were selected for a double-stranded RNA artificial feeding assay. The down-regulated expression levels of LgToll-5-1a and LgPGRP-LB2a resulted in relatively high larval mortality rates and abnormal development. Our data represent a comprehensive genetic resource for immune-related SPB genes, and may contribute to the elucidation of the mechanism regulating innate immunity in Lepidoptera species. Furthermore, two immune-related SPB genes were identified as potential RNAi targets, which may be used in the development of RNAi-mediated SPB control methods.

scholarly journals RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae)

2018 ◽  
Author(s):  
Ruixue Ran ◽  
Tianyu Li ◽  
Xinxin Liu ◽  
Hejia Ni ◽  
Wenbin Li ◽  
...  
Keyword(s):  
Rna Interference ◽  
Larval Mortality ◽  
Crop Protection ◽  
Abnormal Development ◽  
Signalling Molecules ◽  
Rnai Technology ◽  
Pod Borer ◽  
Soybean Pest ◽  
Protection Strategies ◽  
Immune Related Genes

RNA interference (RNAi) technology may be useful for developing new crop protection strategies against the soybean pod borer (SPB; Leguminivora glycinivorella), which is a critical soybean pest in northeastern Asia. Immune-related genes have been recently identified as potential RNAi targets for controlling insects. However, little is known about these genes or mechanisms underlying their expression in the SPB. In this study, we completed a transcriptome-wide analysis of SPB immune-related genes. We identified 41 genes associated with SPB microbial recognition proteins, immune-related effectors or signalling molecules in immune response pathways (e.g., Toll and immune deficiency pathways). Eleven of these genes were selected for a double-stranded RNA artificial feeding assay. The down-regulated expression levels of LgToll-5-1a and LgPGRP-LB2a resulted in relatively high larval mortality rates and abnormal development. Our data represent a comprehensive genetic resource for immune-related SPB genes, and may contribute to the elucidation of the mechanism regulating innate immunity in Lepidoptera species. Furthermore, two immune-related SPB genes were identified as potential RNAi targets, which may be used in the development of RNAi-mediated SPB control methods.

scholarly journals RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae)

2018 ◽  
Author(s):  
Ruixue Ran ◽  
Tianyu Li ◽  
Xinxin Liu ◽  
Hejia Ni ◽  
Wenbin Li ◽  
...  
Keyword(s):  
Rna Interference ◽  
Larval Mortality ◽  
Crop Protection ◽  
Abnormal Development ◽  
Signalling Molecules ◽  
Rnai Technology ◽  
Pod Borer ◽  
Soybean Pest ◽  
Protection Strategies ◽  
Immune Related Genes

RNA interference (RNAi) technology may be useful for developing new crop protection strategies against the soybean pod borer (SPB; Leguminivora glycinivorella), which is a critical soybean pest in northeastern Asia. Immune-related genes have been recently identified as potential RNAi targets for controlling insects. However, little is known about these genes or mechanisms underlying their expression in the SPB. In this study, we completed a transcriptome-wide analysis of SPB immune-related genes. We identified 41 genes associated with SPB microbial recognition proteins, immune-related effectors or signalling molecules in immune response pathways (e.g., Toll and immune deficiency pathways). Eleven of these genes were selected for a double-stranded RNA artificial feeding assay. The down-regulated expression levels of LgToll-5-1a and LgPGRP-LB2a resulted in relatively high larval mortality rates and abnormal development. Our data represent a comprehensive genetic resource for immune-related SPB genes, and may contribute to the elucidation of the mechanism regulating innate immunity in Lepidoptera species. Furthermore, two immune-related SPB genes were identified as potential RNAi targets, which may be used in the development of RNAi-mediated SPB control methods.

scholarly journals RNAi technology for plant protection and its application in wheat

aBIOTECH ◽  
2021 ◽  
Author(s):  
Shaoshuai Liu ◽  
Shuaifeng Geng ◽  
Aili Li ◽  
Yingbo Mao ◽  
Long Mao
Keyword(s):  
Rna Interference ◽  
Future Development ◽  
Recent Progress ◽  
Plant Protection ◽  
Crop Protection ◽  
Significant Progress ◽  
Target Mrnas ◽  
Rnai Technology ◽  
The World ◽  
Made In

AbstractThe RNAi technology takes advantage of the intrinsic RNA interference (RNAi) mechanism that exists in nearly all eukaryotes in which target mRNAs are degraded or functionally suppressed. Significant progress has been made in recent years where RNAi technology is applied to several crops and economic plants for protection against diseases like fungi, pests, and nematode. RNAi technology is also applied in controlling pathogen damages in wheat, one of the most important crops in the world. In this review, we first give a brief introduction of the RNAi technology and the underneath mechanism. We then review the recent progress of its utilization in crops, particular wheat. Finally, we discuss the existing challenges and prospect future development of this technology in crop protection.

Application of the RNA Interference (RNAi) Technology to Angiogenesis Research

2004 ◽  
pp. 167-176
Author(s):  
Sung-Suk Chae ◽  
Ji-Hye Paik ◽  
Jonathan Shubert-Coleman ◽  
Henry Furneaux ◽  
Timothy Hla
Keyword(s):  
Rna Interference ◽  
Rnai Technology

Harnessing chemical ecology for environment-friendly crop protection

2021 ◽  
Author(s):  
Seogchan Kang ◽  
Rhea Lumactud ◽  
Ningxiao Li ◽  
Terrence H Bell ◽  
HyeSeon Kim ◽  
...  
Keyword(s):  
Disease Control ◽  
Large Scale ◽  
Crop Protection ◽  
Bioactive Metabolites ◽  
Small Scale ◽  
Environment Friendly ◽  
Protection Strategies ◽  
Health Related ◽  
Microbe Interactions ◽  
Synthetic Pesticides

Heavy reliance on synthetic pesticides for crop protection becomes increasingly unsustainable, calling for robust alternative strategies that do not degrade the environment and vital ecosystem services. There exist numerous reports of successful disease control using various microbes in small-scale trials. However, their inconsistent efficacy has hampered large-scale applications. An enhanced understanding of how beneficial microbes interact with plants, other microbes, and the environment and which factors affect their efficacy of disease control is crucial to deploy microbial allies as effective and reliable pesticide alternatives. Diverse metabolites produced by plants and microbes participate in pathogenesis and defense, regulate the growth and development of themselves and neighboring organisms, help maintain cellular homeostasis under varied environmental conditions, and affect the assembly and activity of plant and soil microbiomes. However, research on the metabolites associated with plant growth/health-related processes, except antibiotics, has not received adequate attention. This review highlights several classes of metabolites known or suspected to affect plant health, focusing on those associated with biocontrol and belowground plant-microbe and microbe-microbe interactions. The review also presents how new insights anticipated from systematically exploring the diversity and mechanism of action of bioactive metabolites can be harnessed to develop novel crop protection strategies.

The economics of RNAi-based innovation: from the innovation landscape to consumer acceptance.

2021 ◽  
pp. 159-166
Author(s):  
Vera Ventura ◽  
Dario G. Frisio
Keyword(s):  
Rna Interference ◽  
Gene Silencing ◽  
Crop Protection ◽  
Consumer Perceptions ◽  
Market Potential ◽  
Consumer Acceptance ◽  
Innovative Technology ◽  
Sustainable Solutions

Abstract RNA interference (RNAi) is an innovative technology of gene silencing which offers great opportunities for the development of sustainable solutions for crop protection. This chapter discusses the market potential of RNAi innovation, the application of RNAi for biocontrol, and stakeholder and consumer perceptions of RNAi technologies.

scholarly journals Bacterium-Mediated RNA Interference: Potential Application in Plant Protection

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 572 ◽  
Author(s):  
Simon Goodfellow ◽  
Daai Zhang ◽  
Ming-Bo Wang ◽  
Ren Zhang
Keyword(s):  
Rna Interference ◽  
Potential Application ◽  
Plant Protection ◽  
Crop Protection ◽  
Environmentally Friendly ◽  
Agricultural Pests ◽  
Promising Tool ◽  
Current State ◽  
Potential Applications

RNAi has emerged as a promising tool for targeting agricultural pests and pathogens and could provide an environmentally friendly alternative to traditional means of control. However, the deployment of this technology is still limited by a lack of suitable exogenous- or externally applied delivery mechanisms. Numerous means of overcoming this limitation are being explored. One such method, bacterium-mediated RNA interference, or bmRNAi, has been explored in other systems and shows great potential for application to agriculture. Here, we review the current state of bmRNAi, examine the technical limitations and possible improvements, and discuss its potential applications in crop protection.

scholarly journals Functional Analysis of RNA Interference-Related Soybean Pod Borer (Lepidoptera) Genes Based on Transcriptome Sequences

2018 ◽  
Vol 9 ◽  
Author(s):  
Fanli Meng ◽  
Mingyu Yang ◽  
Yang Li ◽  
Tianyu Li ◽  
Xinxin Liu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Rna Interference ◽  
Pod Borer ◽  
Transcriptome Sequences
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