scholarly journals PROXIMATE COMPOSITION, STRUCTURAL CHARACTERIZATION AND PHYTOCHEMICAL SCREENING OF THE SEED OIL OF ADENANTHERA PAVONINA LINN

2016 ◽  
Vol 11 (6) ◽  
pp. 253-263 ◽  
Author(s):  
Olayinka O. Ajani ◽  
Taiwo F. Owoeye ◽  
Grace I. Olasehinde ◽  
Oluwatosin Y. Audu ◽  
Fisayo E. Owolabi ◽  
...  

2016 ◽  
Vol 16 (4) ◽  
pp. 102-111 ◽  
Author(s):  
Olayinka O. Ajani ◽  
Taiwo F. Owoeye ◽  
Grace I. Olasehinde ◽  
Deborah K. Akinlabu ◽  
Fisayo E. Owolabi ◽  
...  

Author(s):  
RAPHAEL C MORDI ◽  
GRACE I OLASEHINDE ◽  
ADEBOLA P OKEDERE ◽  
ARNOLD N ELEGWULE ◽  
JOAN I AYO-AJAYI ◽  
...  

Objectives: Dacryodes edulis is a versatile plant in many African countries, as its various parts are employed to treat several diseases. Like most plants used in traditional medicine, the possible mechanism by which D. edulis functions is still unknown. This study was designed to investigate the components of the plant seed with a view of justifying its use as traditional medicine. Methods: The seed oil of D. edulis was exhaustively extracted with a Soxhlet extractor from 500 g seeds and 200 g seeds of D. edulis using ethanol and petroleum ether as solvent, respectively. The extraction solvent was removed to obtain the oil which was then subjected to antimicrobial activity test to determine its activity against the following clinical isolates namely Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, and Escherichia coli using gentamycin as positive control. Phytochemical screening and gas chromatography-mass spectrometry (GC-MS) analyses were carried out following standard methods Results: Ethanolic extract resulted in a higher percentage of oil yield (11.6%) than petroleum ether (5.3%). D. edulis seed oil showed remarkable activity against Gram-negative and Gram-positive isolates: E. coli, Bacillus spp, and S. aureus but not against P. aeruginosa. The presence of saponins, quinones, cardiac glycosides, terpenoids, and phenol was confirmed during qualitative phytochemical screening, and the preliminary results from GC-MS analysis show the presence of terpinen-4-ol, 4,6,6-trimethyl bicyclo[3.1.1]hept-3-en-2-one, ethyl 14-methyl-hexadecanoate, methyl 19-methyl-eicosanoate, squalene, C-14 to C-18 fatty acids, and their esters. Conclusion: The study, therefore, confirms that the use of D. edulis as component of traditional medicine may be justified.


2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Sewara J. Mohammed ◽  
Hassan H. H. Amin ◽  
Shujahadeen B. Aziz ◽  
Aram M. Sha ◽  
Sarwar Hassan ◽  
...  

This study was aimed to investigate the structure of bioactive components of black seed oil (BSO) and their antimicrobial and cytotoxic effects. Initially, the structural examination was conducted using various spectroscopic techniques, such as FTIR, TLC, and UV-visible spectroscopy, which are important in determining substituents, functional groups, and the presence of conjugated double bonds in BSO. From the FTIR spectra, a variety of sharp, strong, and weak peaks were specified relating to the main components of thymoquinone (TQ), dithymoquinone, thymohydroquinone, and thymol in BSO. The results of UV-visible spectroscopy confirmed the presence of thymoquinone as a major compound, and conjugated double bonds were also found. In addition, qualitative TLC analysis was used to identify thymoquinone from the methanol-extracted layer in BSO, by calculating the retention factor (Rf) value. Furthermore, antimicrobial activity of BSO was studied against various types of bacteria. Strong bacterial inhibitory effects were observed, especially against Bacillus subtilis, with an average inhibition zone of 15.74 mm. Moreover, through the use of the MTT assay in vitro, it was shown that BSO does not exhibit any cytotoxicity towards human peripheral blood mononuclear cells (PBMCs). It was also found from the structural characterization of BSO that the existence of TQ is responsible for potential antibacterial activity without any cytotoxic effects. The main observation of this work is that BSO has antimicrobial activity even against methicillin-resistant Staphylococcus aureus (MRSA).


2019 ◽  
Vol 1 ◽  
pp. 108-113
Author(s):  
B A Anhwange ◽  
I G Agbidye ◽  
B A Kyenge ◽  
P O Ngbede

The leaves of Jatropha tanjarensis were collected and assessed for their phytochemical compositions and antimicrobial activities using methanolic and hexane extracts as to determine the bioactive components and their effects on microorganisms. Also, the nutritional potential was investigated through the determination of proximate composition using standard procedures. The result of phytochemical screening indicated the presence of saponins, cardiac glycosides, reducing sugars, steroids, alkaloids and flavonoids in methanolic extract, the same observation was recorded in n-hexane extract except that reducing sugars were absent and phlobotannins were present. The antimicrobial activities of the leaves extract show that it have some antimicrobial properties. Results of the proximate composition and mineral analysis revealed that the plant is a good source of fiber and minerals. Therefore, leaves of Jatropha tanjarensisapart from being used as antimicrobial agents, can serve as good sources of fiber and minerals when used as vegetables.


2021 ◽  
Vol 21 (3) ◽  
pp. 546
Author(s):  
Nadia Toudert ◽  
Farida Zakkad ◽  
Noureddine Dadda ◽  
Abdelouaheb Djilani ◽  
Amadou Dicko ◽  
...  

Euphorbia species possess pharmacological properties that have been widely used for medical purposes worldwide. In this paper, three plants belonging to the Euphorbia genus growing in North-East of Algeria were studied. The phenolic contents were identified using LC-MS, while the fatty acid composition of their fixed oils was determined with GC-MS. The quantification of the total condensed tannins and the leaves' entire anthocyanin content were performed using photometric methods. The main constituents of the polyphenolic compounds identified by LC-MS were ascorbic, chlorogenic, and ellagic acids. The oil yield of the seeds of E. terracina, E. biumbellata, and E. dendroides was 17.48%, 18.5%, and 20.05%, respectively. Quantitative analyses of these oils using GC-MS showed variations in the species' fatty acid constituents' concentrations and compositions. Besides, the phytochemical screening results showed that E. terracina possessed a high amount of tannin and anthocyanin content compared with other studied plants.


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