Proximate Composition, Qualitative and Quantitative Phytochemical Screening of Aqeous Extract of Aspilia africana (Asteraceae) C.D Adams Leaves

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

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Phytopharmacognostical and Chromatographic Evaluation of Leaves of Launaea procumbens

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00746 ◽

2021 ◽

pp. 4291-4299

Author(s):

Brijesh Charadva ◽

Urvashi Ghataliya ◽

Pooja Meena ◽

Krishna Karia ◽

Tixa Lakhlani ◽

...

Keyword(s):

Solvent System ◽

Acid Anhydride ◽

Phase System ◽

Phytochemical Screening ◽

Calcium Oxalate Crystal ◽

Quantitative Microscopy ◽

Qualitative And Quantitative ◽

Anomocytic Stomata ◽

Chloroform Methanol ◽

Launaea Procumbens

Launaea procumbens leaves are galactagogue, diuretic, antifungal, anorexic, anti-arthritic and hepatoprotective according to Ayurvedic texts as well as modern research. Launaea species, particularly L. pinnatifida, is mentioned as one of the sources of Gojihva, regarding which there is controversy with respect to its true botanical identity. However, no detailed anatomical, phytochemical or chromatographic investigation is available for leaf of L. procumbens which can differentiate it from L. pinnatifida. The aim of this work is to develop standardization parameters of L. procumbens leaves by performing its pharmacognostical evaluation, preliminary phytochemical screening, HPTLC and GC-MS fingerprints. Pharmacognostic investigation of the leaves was performed by its morphological study, qualitative and quantitative microscopy as well as powder microscopy. Extraction of leaves was done by maceration using methanol. This extract was used for preliminary phytochemical screening and chemoprofiling by GC-MS, as well as for developing its HPTLC fingerprint. A mobile phase system was developed by pilot TLC, following which an HPTLC fingerprint was performed using the solvent system chloroform: methanol: ethyl acetate (3:7:6). Diagnostic microscopic characters identified in powder include unicellular covering trichome, xylem vessels, anomocytic stomata, and prisms of calcium oxalate crystal. Phytochemical screening revealed the presence of phytoconstituents classes like phenolics, anthraquinones, saponin glycosides, carbohydrates, sterols, triterpenoids and flavonoids. HPTLC fingerprinting detected 6 peaks with Rf 0.25, 0.37, 0.41, 0.50, 0.65, 0.78 at 254nm and 4 peaks with Rf 0.32, 0.37, 0.50, 0.66 at 366nm. GC-MS fingerprint revealed presence of propanoic acid anhydride, valeric anhydride, 2- Pyrrolidine acetic acid, phthalan, 5- (Hydroxy methyl)-2-(dimethoxy methyl) furan, vanillin, methyl β-l-Arabino pyranoside, 1,6-anhydro-β-D-Gluco pyranose and 6-Methyl-2-Heptanone, 6-(3,5-dimethyl-2-furanyl). Present study will be very useful for herbal industry in differentiating Launaea procumbens from other species of the genus Launaea, particularly L. pinnatifida (Gojihva) as well as for authentication, standardization and detection of adulteration in the leaf formulations of Launaea procumbens.

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PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i6.17803 ◽

2017 ◽

Vol 10 (6) ◽

pp. 225 ◽

Cited By ~ 1

Author(s):

María Cristina Furrianca ◽

Marysol Alvear ◽

Tomás Zambrano ◽

Víctor Fajardo ◽

Luis Salazar

Keyword(s):

Secondary Metabolites ◽

Cardiac Glycosides ◽

Methanolic Extract ◽

Ethanolic Extract ◽

Phytochemical Screening ◽

Qualitative And Quantitative ◽

Medicinal Properties ◽

Phytochemical Constituents ◽

First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

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Proximate composition, amino acid profile and phytochemical screening of Lophira Lanceolataseeds

African Journal of Food Agriculture Nutrition and Development ◽

10.4314/ajfand.v10i1.51476 ◽

2010 ◽

Vol 10 (1) ◽

Cited By ~ 1

Author(s):

SA Lohlum

Keyword(s):

Amino Acid ◽

Proximate Composition ◽

Amino Acid Profile ◽

Phytochemical Screening ◽

Lophira Lanceolata

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Phytochemical Screening, Antimicrobial Activities and Nutritional content of Jatropha Tanjorensis Leaves

NIGERIAN ANNALS OF PURE AND APPLIED SCIENCES ◽

10.46912/napas.114 ◽

2019 ◽

Vol 1 ◽

pp. 108-113

Author(s):

B A Anhwange ◽

I G Agbidye ◽

B A Kyenge ◽

P O Ngbede

Keyword(s):

Proximate Composition ◽

Antimicrobial Agents ◽

Reducing Sugars ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Phytochemical Screening ◽

Bioactive Components ◽

Standard Procedures ◽

Hexane Extracts

The leaves of Jatropha tanjarensis were collected and assessed for their phytochemical compositions and antimicrobial activities using methanolic and hexane extracts as to determine the bioactive components and their effects on microorganisms. Also, the nutritional potential was investigated through the determination of proximate composition using standard procedures. The result of phytochemical screening indicated the presence of saponins, cardiac glycosides, reducing sugars, steroids, alkaloids and flavonoids in methanolic extract, the same observation was recorded in n-hexane extract except that reducing sugars were absent and phlobotannins were present. The antimicrobial activities of the leaves extract show that it have some antimicrobial properties. Results of the proximate composition and mineral analysis revealed that the plant is a good source of fiber and minerals. Therefore, leaves of Jatropha tanjarensisapart from being used as antimicrobial agents, can serve as good sources of fiber and minerals when used as vegetables.

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SKRINING FITOKIMIA DAN KANDUNGAN TOTAL FLAVANOID PADA BUAH Carica pubescens Lenne & K. Koch DI KAWASAN BROMO, CANGAR, DAN DATARAN TINGGI DIENG

el–Hayah ◽

10.18860/elha.v5i2.3022 ◽

2015 ◽

Vol 5 (2) ◽

pp. 73 ◽

Cited By ~ 1

Author(s):

Eko Budi Minarno

Keyword(s):

Positive Sample ◽

Screening Tests ◽

Total Flavonoids ◽

Test Results ◽

Phytochemical Screening ◽

Qualitative And Quantitative ◽

The Third ◽

Fruit Samples ◽

The Tropics ◽

Carica Pubescens

Carica pubescens Lenne & K. Koch is one of the species in the tropics, which adapt to the plateau environment and low temperatures. In Indonesia, this plant is found at Cangar, Bromo, and Dieng Plateau. This study aims to determine the results of phytochemical screening and total flavonoids in fruit samples were taken from the third place. Qualitative and quantitative tests carried out in the Laboratory of Department of Biology and Chemistry, Faculty of Science and Technology, Maulana Malik Ibrahim State Islamic University, Malang. Analysis of total flavonoids using a spectrophotometer at λ = 510 nm. The results of qualitative phytochemical screening tests on samples of C. pubescens fruits at Cangar, Bromo, and Dieng Plateau shows that the positive sample contains flavonoids, polyphenols, tannins, and triterpenoids. Quantitative test results show that the C. pubescens at Cangar contains total flavonoids quercetin equivalent with value 800 mg / L, Bromo with value 816.65 mg / L, and Dieng Plateau with value 633.35 mg / L, respectively.

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Qualitative and Quantitative Phytochemical Composition of Brachystegia Eurycoma Root, Leaf and Stem Bark

Advances in Bioengineering and Biomedical Science Research ◽

10.33140/abbsr.03.02.01 ◽

2020 ◽

Vol 3 (2) ◽

Keyword(s):

Standard Procedure ◽

Stem Bark ◽

Phytochemical Screening ◽

Steroid Saponin ◽

Root Extracts ◽

Qualitative And Quantitative ◽

Aqueous Solvent ◽

Medicinal Properties ◽

Phytochemical Constituents ◽

High Concentrations

This study assesses the phytochemical screening of Brachystegia eurycoma root, leaf and bark using aqueous solvent. Phytochemical screening was conducted to determine the presence of natural products (alkaloids, tannins, steroid, saponin, phenol, carbohydrate, flavonoid, terpenoid, triterpenoids, quinone and cardial glycoside) using standard procedure. The qualitative phytochemical constituents of Brachystegia eurycoma leaf, root and stem bark revealed the presence Alkaloid, Flavonoid, quinone, tannin, saponin and Terpenoids while Phenol, carbohydrate, steroids and triterpenoids are absent in the leaf, stem bark and root extracts of the plant. Cardiac glycoside was only present in root extracts. The quantitative phytochemical screening of the extracts showed that they had high concentrations of alkaloids and tannins and they were relatively low in Terpenoids. The results evidently specifies aqueous extract of leaf, bark and root of Brachystegia eurycoma contains various bioactive compounds which have various medicinal properties that can be used for the treatment of many diseases. However, isolation of individual phytochemical constituents and subjecting it to the biological activity will definitely provide more fruitful results. Therefore, it is recommended as a plant of phytopharmaceutical importance.

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