scholarly journals Characterizations of novel pesticide-degrading bacterial strains from industrial wastes found in the industrial cities of Pakistan and their biodegradation potential

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12211
Author(s):  
Noreen Asim ◽  
Mahreen Hassan ◽  
Farheen Shafique ◽  
Maham Ali ◽  
Hina Nayab ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Industrial Wastes ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
16S Rrna Analysis ◽  
Degrading Bacteria ◽  
Considerable Degradation ◽  
Different Strains

Background Lack of infrastructure for disposal of effluents in industries leads to severe pollution of natural resources in developing countries. These pollutants accompanied by solid waste are equally hazardous to biological growth. Natural attenuation of these pollutants was evidenced that involved degradation by native microbial communities. The current study encompasses the isolation of pesticide-degrading bacteria from the vicinity of pesticide manufacturing industries. Methods The isolation and identification of biodegrading microbes was done. An enrichment culture technique was used to isolate the selected pesticide-degrading bacteria from industrial waste. Results Around 20 different strains were isolated, among which six isolates showed significant pesticide biodegrading activity. After 16S rRNA analysis, two isolated bacteria were identified as Acinetobacter baumannii (5B) and Acidothiobacillus ferroxidans, and the remaining four were identified as different strains of Pseudomonas aeruginosa (1A, 2B, 3C, 4D). Phylogenetic analysis confirmed their evolution from a common ancestor. All strains showed distinctive degradation ability up to 36 hours. The Pseudomonas aeruginosa strains 1A and 4D showed highest degradation percentage of about 80% for DDT, and P. aeruginosa strain 3C showed highest degradation percentage, i.e., 78% for aldrin whilst in the case of malathion, A. baumannii and A. ferroxidans have shown considerable degradation percentages of 53% and 54%, respectively. Overall, the degradation trend showed that all the selected strains can utilize the given pesticides as sole carbon energy sources even at a concentration of 50 mg/mL. Conclusion This study provided strong evidence for utilizing these strains to remove persistent residual pesticide; thus, it gives potential for soil treatment and restoration.

scholarly journals Isolation and Molecular Characterization of Pesticide Degrading Bacteria in Polluted Soil

2020 ◽  
Author(s):  
Kehinde Sowunmi ◽  
Suliat Morenike Shoga ◽  
Oluwabukola Mabel Adewunmi ◽  
Adewale Felix Oriyomi ◽  
Lukman Sowunmi
Keyword(s):  
Enrichment Culture ◽  
Lethal Effect ◽  
Living System ◽  
Culture Technique ◽  
Bacterial Strains ◽  
High Concentration ◽  
Degrading Bacteria ◽  
Quality Check ◽  
Pure Bacterial Cultures

AbstractPesticides are the substances for preventing, destroying, repelling any pest. Due to bulk handling or accidental release, they are accumulated in soil which leads to occasional entry into ecosystem that shows lethal effect on living system. An enrichment culture technique was used to isolate bacterial strains from organophosphate soil degrading high concentration of the selected pesticides. Five pure bacterial cultures were isolated. All five isolates were characterized on the basis of molecular and biochemical features like biodegradation test and substrate specificity, phosphate solubilization and screened for pesticide residue, pH, and extraction of DNA, quantity and quality check and salt tolerance. The organophosphate isolates were also tested for quantitative production. The screening of pesticide tolerance was done at for fungicides and insecticides.

The metabolism of p-fluorophenylacetic acid by a Pseudomonas sp. I. Isolation and identification of intermediates in degradation

1971 ◽  
Vol 17 (5) ◽  
pp. 635-644 ◽  
Author(s):  
D. B. Harper ◽  
E. R. Blakley
Keyword(s):  
Sole Carbon Source ◽  
Culture Medium ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Spectroscopic Techniques ◽  
Resting Cells ◽  
Pseudomonas Sp ◽  
Isolation And Identification ◽  
Hydroxyphenylacetic Acid ◽  
Organic Fluorine

A Pseudomonas sp. capable of growing on p-fluorophenylacetic acid as sole carbon source has been isolated using the enrichment culture technique. All the organic fluorine is released into the culture medium as fluoride ion during growth. A number of fluorinated intermediates have been isolated from the culture medium when resting cells were incubated with the substrate. Using infrared, nuclear magnetic resonance, and mass spectroscopic techniques together with chemical degradative procedures, these have been identified as D(+)-monofluorosuccinic acid, trans-3-fluoro-3-hexenedioic acid, (−)-4-carboxymethyl-4-fluorobutanolide, 4-fluoro-2-hydroxyphenylacetic acid, and 4-fluoro-3-hydroxyphenylacetic acid.

scholarly journals Eksplorasi Bakteri Indigen Pada Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup [Exploration Of Indigen Bacteria From Catfish (Clarias sp.) Breeding On Closed Resirculation System ]

2019 ◽  
Vol 4 (2) ◽  
pp. 193
Author(s):  
Prayogo, Boedi Setya Rahardja, Abdul Manan
Keyword(s):  
Organic Matter ◽  
Organic Materials ◽  
Pseudomonas Stutzeri ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Biological Filtration ◽  
Degrading Bacteria ◽  
Recirculation System ◽  
Pseudomonas Pseudomallei

Abstract The efforts of the catfish hatchery fish are generally confined to the central areas of hatchery that has abundant water resources. Solving the problem of limited water, appears a catfish hatchery system with a closed recirculation system. In such systems the process of biological filtration is the most important thing. It shows the handling of organic materials by utilizing the degrading bacteria is the key to successful management of the system. Thus necessary to be studied the role of bacteria degrading organic matter indigen (local bacteria) in the closed hatchery recirculation system of catfish. This study aimed to obtain bacterial strains degrading bacteria indigen as organic materials are very useful in improving water quality in the closed hatchery recirculation system of catfish and knowing the methods to the use of bacteria indigen as degrading organic matter. The method used in this study was designed based on the stages of research carried out in accordance with the objectives to be achieved. The results of the isolation and identification to the species level showed Pseudomonas stutzeri and Pseudomonas pseudomallei obtain the greatest value for hydrolysis index representing each trait protease, lipase and amylase. Bioremediation effectiveness test in vitro showed effective results in the treatment using consortia of bacteria inoculant. Consortia of bacteria inoculant effective in improving the growth rate and survival rate (SR) in the closed hatchery recirculation system of catfish.

Isolation and identification of low density polythene-degrading bacteria from soil of North West of Algeria

2019 ◽  
Vol 8 (3) ◽  
pp. 76-82
Author(s):  
K. Zerhouni ◽  
B. Abbouni ◽  
K. Kanoun ◽  
K. Larbi Daouadji ◽  
A. Tifrit ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Low Density Polyethylene ◽  
Low Density ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Daily Lives ◽  
North West ◽  
Degrading Bacteria ◽  
Solid Culture Medium ◽  
Chemical Cycling

Plas c bags (Low Density Polyethylene (LDPE) belong to the polymers, which plays a very important role in our daily lives by their diversi ed applica on. However, the accumula on of the plas c bags in the environment cons - tutes a serious problem and a real source for visual nuisance, pollu on of soil and marine environments. Furthermore, their biodegradation was the safest method of breakdown that possibly leaves behind less toxic residues and showed poten al of bio-geo chemical cycling of the substrate. The aim of the present work was the characterization of the isolated bacterial strains from a municipal land ll area of Tlemcen, North West Algeria, which were implicated by the biodegrada on ability of the Low Density Polyethylene. The degradation of the Low Density Polyethylene was inves gated by studying the bacterial growth of the isolated, inoculated on a solid culture medium, which was composed of LDPE as the sole carbon source with and with- out a nitrogen source and the selec on was based by the determination of the produced diameter of hydrolysis clear zone on the surface. Furthermore, the isolated, selected degrading Low Density Polyethylene bacterial ML002 has been iden ed by the study of their morphological, biochemical charac- teris cs and the ampli ca on of the fragment, coding the region of ARN 16S. The use of the API system indicated their belonging to the genus Bacillus Cereus, which has reduced the weight of LDPE by 0.26, 1.28, 1.53% a er 30, 90, 120 days respec vely. Furthermore, the amplified of the fragment, coding the region of ARN 16S by the isolated, selected bacterial ML002 indicated a similarity of 99.394% with Bacillus wiedmannii and Bacillus proteolyticus and 99.293% homology with Bacillus toyonensis, Bacillus cereus and Bacillus thuringiensis.

scholarly journals Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii

2021 ◽  
Vol 16 (3) ◽  
pp. 130-138
Author(s):  
Fazilah Arifin ◽  
Rozilawati Mohamed Razali ◽  
Vigneswari Sevakumaran
Keyword(s):  
16S Rdna ◽  
Anthropogenic Activities ◽  
Morphological Characteristics ◽  
Gravimetric Method ◽  
Diesel Oil ◽  
Plate Count ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Degrading Bacteria ◽  
Diesel Degradation

Marine contamination caused by anthropogenic activities has side effects and causes severe contamination to the environment. Polychaetes are benthic organisms that live in the sediment and can be a good indicator of sediment contamination by organic compounds. In this study, bacterial strains were isolated and identified from the gut of polychaete worm Marphysa moribidii and the potential of the bacteria was evaluated to degrade hydrocarbon compounds. The isolated bacteria were primary and secondary screened on Minimal Salt Media (MSM) agar supplemented with 1% v/v of diesel oil. Diesel degradation analysis was performed by inoculating potential bacterium into MSM broth with 1% v/v diesel oil and incubated at 37 oC for 20 days. Diesel degradation percentage was analyzed using the gravimetric method, while the bacteria cell densities were measured using the standard plate count method. Then, the selected isolates were identified based on their morphological characteristics and 16S rDNA sequences. As a result, two bacteria isolates coded as Isolate 6 and Isolate 8 were able to degrade diesel oil up to 52.29% and 39.24% after 20 days of incubation. The 16S rDNA sequence analysis revealed that it was identified as Bacillus sp. strain UMTFA1 (RB) and Staphylococcus kloosii strain UMTFA2 (RS). Our result showed that these strains have the potential in oil-degrading processes, which will provide new insight into bioremediation process and decrease environmental pollution in soil and water contaminated with hydrocarbons.

scholarly journals Screening, Isolation and Biochemical Characterization of Laccase Producing Bacteria from Contaminated Sites

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Siva Jyothi J. ◽  
Kishore Kumar K. ◽  
Hema Latha E.
Keyword(s):  
Biochemical Characterization ◽  
Agar Medium ◽  
Nutrient Agar ◽  
Laccase Production ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Leading Role ◽  
Degrading Bacteria ◽  
Nutrient Agar Medium ◽  
Guntur District

Screening and isolation of Laccase producing bacteria from Guntur District soil was carried out to assess the diversity of Lignocellulose degrading bacteria. Isolation and identification of environmental friendly bacteria for lignin degradation becomes an essential one, because all the researchers are mainly concentrating on fungal strains. However, bacteria seem to play a leading role in decomposing lignin. For isolation of Laccase producing bacteria nutrient agar medium containing guaiacol was used. Total nine bacterial strains were isolated from soil samples collected from different regions of Guntur district. Preliminary screening of bacterial strains was carried out on guaiacol containing nutrient agar medium for laccase production. Formation of green colour using ABTS (2,2'- azinobis(3-ethylbenzthiazoline-6-sulfonate) confirms the capability of laccase production by the bacterial strains. Nine bacterial strains showed positive results. High laccase producing bacterial isolates were examined for morphological and biochemical characteristics according to Bergey’s Manual of Systematic Bacteriology. The predominant isolates were identified as Bacillus and Enterobacter species.

scholarly journals Glyphosate Utilization as the Source of Carbon: Isolation and Identification of new Bacteria

2011 ◽  
Vol 8 (4) ◽  
pp. 1582-1587 ◽  
Author(s):  
M. Mohsen Nourouzi ◽  
T. G. Chuah ◽  
Thomas S. Y. Choong ◽  
C. J. Lim
Keyword(s):  
Carbon Source ◽  
Stenotrophomonas Maltophilia ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Providencia Alcalifaciens ◽  
Mixed Bacteria ◽  
Aminomethyl Phosphonic Acid

Mixed bacteria from oil palm plantation soil (OPS) were isolated to investigate their ability to utilize glyphosate as carbon source. Results showed that approximately all of the glyphosate was converted to aminomethyl-phosphonic acid (AMPA) (99.5%). It is worthy to note that mixed bacteria were able to degrade only 2% of AMPA to further metabolites. Two bacterial strainsi.e. Stenotrophomonas maltophiliaandProvidencia alcalifacienswere obtained from enrichment culture. Bacterial isolates were cultured individually on glyphosate as a sole carbon source. It was observed that both isolates were able to convert glyphosate to AMPA.

scholarly journals Isolation and Identification of Pesticides Degrading Bacteria from Farmland Soil

2019 ◽  
Vol 35 (2) ◽  
pp. 90-94
Author(s):  
Md Atikur Rahman ◽  
ASM Shamsul Arefin ◽  
Otun Saha ◽  
Md Mizanur Rahaman
Keyword(s):  
16S Rdna ◽  
Restriction Analysis ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Rdna Sequencing ◽  
Degrading Bacteria ◽  
Farmland Soil ◽  
Wide Range ◽  
Dna Restriction

Pesticides are recognized to be the threat to the environment and associated with a wide range of serious diseases including respiratory diseases, cancer and even birth defects. In this study, six-different bacterial strains capable of degrading Carbofuran, Emamectin Benzoate and Thiamethoxam were isolated from eight different soil samples. The isolates were characterized by using different conventional and molecular methods. The strains were identified molecularly into different genotypes using amplified ribosomal DNA restriction analysis (ARDRA) and partial sequencing of 16S rDNA. The ARDRA pattern clustered them into 3 groups. Among the isolates three were identified as Achromobacter spp. and one as Diaphorobacter sp. by biochemical tests. It was further confirmed by the partial 16S rDNA sequencing. The two identified potential bacteria can be used for biodegradation of different pesticides which can have a significant environmental impact in soil farm. Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 90-94

scholarly journals Screening for Urease-Producing Bacteria from Limestone Caves of Sarawak

2016 ◽  
Vol 6 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Armstrong Ighodalo Omoregie ◽  
Nurnajwani Senian ◽  
Phua Ye Li ◽  
Ngu Lock Hei ◽  
Dominic Ong Ek Leong ◽  
...  
Keyword(s):  
Chemical Reaction ◽  
Urease Activity ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Calcite Precipitation ◽  
Microbially Induced Calcite Precipitation ◽  
Key Enzyme ◽  
Urease Production

Urease is a key enzyme in the chemical reaction of microorganism and has been found to be associated withcalcification, which is essential in microbially induced calcite precipitation (MICP) process. Three bacterialisolates (designated as LPB19, TSB31 and TSB12) were among twenty-eight bacteria that were isolated fromsamples collected from Sarawak limestone caves using the enrichment culture technique. Isolates LPB19, TSB31and TSB12 were selected based on their quick urease production when compared to other isolates. Phenotypiccharacteristics indicate all three bacterial strains are gram-positive, rod-shaped, motile, catalase and oxidasepositive. Urease activity of the bacterial isolates were measured through changes in conductivity in the absence ofcalcium ions. The bacterial isolates (LPB19, TSB12 and TSB31) showed urease activity of 16.14, 12.45 and 11.41mM urea hydrolysed/min respectively. The current work suggested that these isolates serves as constitutiveproducers of urease, potentially useful in inducing calcite precipitates.

Sign in / Sign up

Export Citation Format

Share Document