scholarly journals Investigation of lipolytic activity of the red king crab hepatopancreas homogenate by NMR spectroscopy

PeerJ ◽  
2022 ◽  
Vol 10 ◽  
pp. e12742
Author(s):  
Maria Timchenko ◽  
Vladislav Molchanov ◽  
Maxim Molchanov ◽  
Alexander Timchenko ◽  
Evgeny Sogorin
Keyword(s):  
Nmr Spectroscopy ◽  
Lipolytic Activity ◽  
Red King Crab ◽  
Moderate Activity ◽  
Reaction Products ◽  
King Crab ◽  
Crab Hepatopancreas ◽  
Wide Ph Range ◽  
The Moment ◽  
Ph Range

The digestive gland of craboids (hepatopancreas) is rich in a huge number of various enzymes (collagenases, nucleases, hyaluronidases, proteases), which are well studied at the moment. However, little is known about crustacean lipases. In this work, using 1H NMR spectroscopy, it was found that the hepatopancreas homogenate of the red king crab Paralithodes camtschaticus demonstrates high lipolytic activity against triacetin in a wide pH range and shows moderate activity against the caprylic/capric triglyceride emulsion. Under the action of the hepatopancreas homogenate, triacylglycerols are converted into 1,2-diacylglycerol, and then into 2-monoacylglycerol and 1-monoacylglycerol. The 1-monoacylglycerol predominates in the reaction products. The use of NMR spectroscopy makes it possible to quickly detect hydrolysis products and evaluate the reaction direction.

scholarly journals Prospects of Red King Crab Hepatopancreas Processing: Fundamental and Applied Biochemistry

Recycling ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 3
Author(s):  
Tatyana Ponomareva ◽  
Maria Timchenko ◽  
Michael Filippov ◽  
Sergey Lapaev ◽  
Evgeny Sogorin
Keyword(s):  
Soviet Union ◽  
Point Of View ◽  
Ecological Niches ◽  
Red King Crab ◽  
Crab Species ◽  
Production Scale ◽  
King Crab ◽  
The Soviet Union ◽  
Fundamental Point ◽  
Crab Hepatopancreas

Since the early 1980s, a large number of studies on enzymes from the red king crab hepatopancreas were conducted. They have been relevant both from a fundamental point of view in terms of studying the enzymes of marine organisms and in terms of rational natural resource management aimed to obtain new valuable products from the processing of crab fishing waste. Most of these works were performed by Russian scientists due to the area and amount of waste of red king crab processing in Russia (or the Soviet Union). However, the close phylogenetic kinship and the similar ecological niches of commercial crab species and the production scale of the catch provide the bases for the successful transfer of experience in the processing of the red king crab hepatopancreas to other commercial crab species caught worldwide. This review describes the value of recycled commercial crab species, discusses processing problems, and suggests possible solutions for these issues. The main emphasis is made on hepatopancreatic enzymes as the most salubrious products of red king crab waste processing.

scholarly journals Prospects of Red King Crab Hepatopancreas Processing: Fundamental and Applied Biochemistry

2020 ◽  
Author(s):  
Tatyana Ponomareva ◽  
Maria Timchenko ◽  
Michael Filippov ◽  
Sergey Lapaev ◽  
Evgeny Sogorin
Keyword(s):  
Soviet Union ◽  
Point Of View ◽  
Ecological Niches ◽  
Red King Crab ◽  
Crab Species ◽  
Production Scale ◽  
King Crab ◽  
The Soviet Union ◽  
Crab Hepatopancreas ◽  
Rational Management

Since the early 1980s, a large number of research works on enzymes from the red king crab hepatopancreas have been conducted. These studies have been relevant both from a fundamental point of view for studying the enzymes of marine organisms and in terms of the rational management of nature to obtain new and valuable products from the processing of crab fishing waste. Most of these works were performed by Russian scientists due to the area and amount of waste of red king crab processing in Russia (or the Soviet Union). However, the close phylogenetic kinship and the similar ecological niches of commercial crab species and the production scale of the catch provide the bases for the successful transfer of experience in the processing of red king crab hepatopancreas to other commercial crab species mined worldwide. This review describes the value of recycled commercial crab species, discusses processing problems, and suggests possible solutions to these problems. The main emphasis is placed on the enzymes of the hepatopancreas as the most highly salubrious product of waste processed from red king crab fishing.

scholarly journals Perspectives on the Use of Hyaluronidase from the Red King Crab Hepatopancreas in Treating Filler Complications: In Vitro Investigations

2019 ◽  
Author(s):  
Tatyana Ponomareva ◽  
Dmitrii Sliadovskii ◽  
Maria Timchenko ◽  
Maxim Molchanov ◽  
Alexander Timchenko ◽  
...  
Keyword(s):  
Red King Crab ◽  
King Crab ◽  
Force Microscopy ◽  
Atomic Force ◽  
Safe Product ◽  
Crab Hepatopancreas ◽  
Aggregation Formation ◽  
Turbidimetric Analysis ◽  
Filler Complications

This study focused on hydrolysis of cosmetic fillers hyaluronic acid (HA) and kinetics of the HA hydrolysis using the homogenate of the red king crab hepatopancreas. Turbidimetric analysis of the reaction mixture revealed a bell-shaped time dependence of aggregation formation. It was shown that the obtained homogenate has the similar activity to the commercially available hyaluronidase. The atomic force microscopy (AFM) examination found that the HA fillers were represented by spherical-like structures. These structures were destroyed under the action of the homogenate of the red king crab hepatopancreas. NMR of the reaction mixture showed that HA degradation lasts for some days, but a maximum rate of the reaction is detected in the first hours of incubation. The preparation with hyaluronidase activity obtained from the red king crab hepatopancreas could be used as potentially safe product for treating filler complications.

scholarly journals Perspectives on the Use of Hyaluronidase from the Red King Crab Hepatopancreas in Treating Filler Complications: In Vitro Investigations

2019 ◽  
Author(s):  
Tatyana Ponomareva ◽  
Dmitrii Sliadovskii ◽  
Maria Timchenko ◽  
Alexander Timchenko ◽  
Evgeny Sogorin
Keyword(s):  
Red King Crab ◽  
King Crab ◽  
Force Microscopy ◽  
Atomic Force ◽  
Safe Product ◽  
Crab Hepatopancreas ◽  
Aggregation Formation ◽  
First Time ◽  
Filler Complications

The kinetics of the hydrolysis of hyaluronic acid (HA) of cosmetic fillers using thehomogenate of the red king crab hepatopancreas was studied for the first time. Turbidimetricanalysis of the reaction mixture revealed a bell–shaped time dependence of aggregation formation. The HA fillers were examined by atomic force microscopy (AFM) and it was found that they wererepresented by spherical–like structures. These structures were disrupted under the action of thehomogenate of the red king crab hepatopancreas. It was shown that the prepared homogenate hasthe activity which is similar to that observed in the commercially available hyaluronidase products.The preparation with hyaluronidase activity obtained from the red king crab hepatopancreas couldbe used as potentially safe product for treating filler complications.

scholarly journals Multifunctional Composition Based on Surfactants and a Complex of Buffer Systems to Enhance Oil Recovery of High-Viscosity Oil Deposits

2021 ◽  
pp. 30-37
Author(s):  
Liubov K. Altunina ◽  
Vladimir A. Kuvshinov ◽  
Ivan V. Kuvshinov ◽  
Liubov A. Stasyeva
Keyword(s):  
Oil Recovery ◽  
High Viscosity ◽  
Buffer System ◽  
Reaction Products ◽  
Wide Ph Range ◽  
Low Interfacial Tension ◽  
Oil Deposits ◽  
Ph Range ◽  
Enhance Oil Recovery ◽  
High Viscosity Oil

To enhance oil recovery of high-viscosity oil deposits in the temperature range of 20-210 °C, a multifunctional composition with adjustable viscosity and high oil-displacing ability has been developed based on surfactants, aluminum and ammonium salts, an adduct of inorganic acid, carbamide and polyol. High buffer capacity of the composition in a wide pH range, 2.5-10 units pH, is provided by a complex of buffer systems: in the acidic range of pH, 2.5-4.0, the system “polyolboric acid and its salt”, in the alkaline range of pH – anammonia-borate buffer system. The composition has a low interfacial tension, a prolonged reaction with carbonate rocks, prevents the precipitation of insoluble reaction products, and increases the permeability of reservoirs. The composition is both oil-displacing and flow-diverting, it provides an enhance in the oil recovery factor, both due to the increase in the displacement factor and the the reservoir sweep by waterflooding or thermal steam stimulation

scholarly journals The effect of hepatopancreas homogenate of the Red king crab on HA-based filler

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8579 ◽  
Author(s):  
Tatyana Ponomareva ◽  
Dmitrii Sliadovskii ◽  
Maria Timchenko ◽  
Maxim Molchanov ◽  
Alexander Timchenko ◽  
...  
Keyword(s):  
Red King Crab ◽  
King Crab ◽  
Force Microscopy ◽  
Atomic Force ◽  
Hydrolysis Process ◽  
Crab Hepatopancreas ◽  
Turbidimetric Analysis ◽  
Hydrolysis Of ◽  
Microscopy Images ◽  
Filler Complications

In this study, several methods were used to analyze the hydrolysis of hyaluronic acid (HA)-based cosmetic fillers by the hepatopancreas homogenate of the Red king crab. The results show that the homogenate and commercially available hyaluronidases have similar hydrolysis activities on the fillers. Atomic force microscopy images reveal that the HA fillers consist mainly of spherical-like particles, which are converted into filamentous structures as a result of hydrolysis by the Red king crab hepatopancreas homogenate. Turbidimetric analysis of the hydrolysis process shows that HA aggregation with acidic albumin exhibits a bell-shaped dependence on reaction time. Analysis of the hydrolysis process by nuclear magnetic resonance shows that HA degradation lasts several days. The maximum rate of the reaction is detected in the 1st h of incubation. The data confirm that the purified homogenate of the Red king crab hepatopancreas exerts hyaluronidase activity on HA-based cosmetic fillers; therefore, it may be considered as a potential therapeutic agent for treating filler complications.

scholarly journals Overexpression and Biochemical Characterization of an Endo-α-1,4-polygalacturonase from Aspergillus nidulans in Pichia pastoris

2020 ◽  
Vol 21 (6) ◽  
pp. 2100
Author(s):  
Hua Xu ◽  
Pengfei Zhang ◽  
Yuchen Zhang ◽  
Zebin Liu ◽  
Xuebing Zhang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Aspergillus Nidulans ◽  
Biochemical Characterization ◽  
Structural Characteristics ◽  
Maximum Activity ◽  
Feed Additives ◽  
Moderate Activity ◽  
Multiple Sequence ◽  
Wide Ph Range ◽  
Ph Range

Pectinases have many applications in the industry of food, paper, and textiles, therefore finding novel polygalacturonases is required. Multiple sequence alignment and phylogenetic analysis of AnEPG (an endo-α-1,4-polygalacturonase from Aspergillus nidulans) and other GH 28 endo-polygalacturonases suggested that AnEPG is different from others. AnEPG overexpressed in Pichia pastoris was characterized. AnEPG showed the highest activity at pH 4.0, and exhibited moderate activity over a narrow pH range (pH 2.0–5.0) and superior stability in a wide pH range (pH 2.0–12.0). It displayed the highest activity at 60 °C, and retained >42.2% of maximum activity between 20 and 80 °C. It was stable below 40 °C and lost activity very quickly above 50 °C. Its apparent kinetic parameters against PGA (polygalacturonic acid) were determined, with the Km and kcat values of 8.3 mg/mL and 5640 μmol/min/mg, respectively. Ba2+ and Ni2+ enhanced activity by 12.2% and 9.4%, respectively, while Ca2+, Cu2+, and Mn2+ inhibited activity by 14.8%, 12.8%, and 10.2% separately. Analysis of hydrolysis products by AnEPG proved that AnEPG belongs to an endo-polygalacturonase. Modelled structure of AnEPG by I-TASSER showed structural characteristics of endo-polygalacturonases. This pectinase has great potential to be used in food industry and as feed additives.

Polarographic and spectrophotometric behaviour of some N-p-phenyl substituted benzamidines

1991 ◽  
Vol 56 (12) ◽  
pp. 2791-2799 ◽  
Author(s):  
Juan A. Squella ◽  
Luis J. Nuñez-Vergara ◽  
Hernan Rodríguez ◽  
Amelia Márquez ◽  
Jose M. Rodríguez-Mellado ◽  
...  
Keyword(s):  
Spectrophotometric Study ◽  
Absorption Bands ◽  
Electrochemical Parameters ◽  
Wide Ph Range ◽  
Group A ◽  
Substituent Constants ◽  
Ph Range ◽  
The Waves

Five N-p-phenyl substituted benzamidines were studied by DC and DP polarography in a wide pH range. Coulometric results show that the overall processes are four-electron reductions. Logarithmic analysis of the waves indicate that the process are irreversible. The influence of the pH on the polarographic parameters was also studied. A UV spectrophotometric study was performed in the pH range 2-13. In basic media some variations in the absorption bands were observed due to the dissociation of the amidine group. A determination of the pK values was made by deconvolution of the spectra. Correlations of both the electrochemical parameters and spectrophotometric pK values with the Hammett substituent constants were obtained.

Sign in / Sign up

Export Citation Format

Share Document