A review of Euryoryzomys legatus (Rodentia, Sigmodontinae): morphological redescription, cytogenetics, and molecular phylogeny

The taxonomic history of Euryoryzomys legatus has been complex and controversial, being either included in the synonymy of other oryzomyine species or considered as a valid species, as in the most recent review of the genus. Previous phylogenetic analyses segregated E. legatus from E. russatus, its putative senior synonym, but recovered it nested within E. nitidus. A general lack of authoritative evaluation of morphological attributes, details of the chromosome complement, or other data types has hampered the ability to choose among alternative taxonomic hypotheses, and thus reach a general consensus for the status of the taxon. Herein we revisit the status of E. legatus using an integrated approach that includes: (1) a morphological review, especially centered on specimens from northwestern Argentina not examined previously, (2) comparative cytogenetics, and (3) phylogenetic reconstruction, using mitochondrial genes. Euryoryzomys legatus is morphologically and phylogenetically distinct from all other species-level taxa in the genus, but its 2n=80, FN=86 karyotype is shared with E. emmonsae, E. nitidus, and E. russatus. Several morphological and morphometric characters distinguish E. legatus from other species of Euryoryzomys, and we provide an amended diagnosis for the species. Morphological characters useful in distinguishing E. legatus from E. nitidus, its sister taxon following molecular analyses, include: larger overall size, dorsal fur with a strong yellowish brown to orange brown tinge, flanks and cheeks with an orange lateral line, ventral color grayish-white with pure white hairs present only on the chin, presence of a thin blackish eye-ring, tail bicolored, presence of an alisphenoid strut and a well-developed temporal and lambdoid crests in the skull, and a labial cingulum on M3. Molecular phylogenetic analyses recovered E. legatus as a monophyletic group with high support nested within a paraphyletic E. nitidus; genetic distances segregated members of both species, except for an exemplar of E. nitidus. Our integrated analyses reinforce E. legatus as a full species, but highlight that E. macconnelli, E. emmonsae, and E. nitidus each may be a species complex and worthy of systematic attention. Finally, we also evaluated the chromosome evolution of the genus within a phylogenetic context.

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Complement to the knowledge of the Haploops species (Crustacea, Gammaridea, Ampeliscidae), with the description of two new species from North Atlantic Ocean. [Contribution to the knowledge of the Haploops genus. 10.]

Zootaxa ◽

10.11646/zootaxa.5048.2.1 ◽

2021 ◽

Vol 5048 (2) ◽

pp. 151-175

Author(s):

R.A. KAIM-MALKA ◽

D. BELLAN-SANTINI ◽

J.C. DAUVIN

Keyword(s):

Atlantic Ocean ◽

North Atlantic ◽

North Atlantic Ocean ◽

Morphological Characters ◽

Valid Species ◽

Single Pair ◽

The North ◽

Two New Species ◽

The Status ◽

The North Atlantic

Two new Haploops species are described from the North Atlantic Ocean: a blind species Haploops faroensis spec. nov. and Haploops truncata spec. nov. with a single pair of corneal lenses. In addition, Haploops vallifera Stephensen 1925 and Haploops similis Stephensen 1925, are re-described and the status of Haploops spinosa Shoemaker 1931, is re-established as a valid species. A table is given of the 75 morphological characters of the studied species.

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A curious nematode, Diploscapteroides persicus n. sp. (Nematoda: Rhabditida: Rhabditomorpha), from Iran with a note on the status of the male genital papillae

Nematology ◽

10.1163/15685411-00002922 ◽

2015 ◽

Vol 17 (9) ◽

pp. 1045-1056 ◽

Cited By ~ 1

Author(s):

Razieh Ghaemi ◽

Ebrahim Pourjam ◽

Sergio Álvarez-Ortega ◽

Majid Pedram ◽

Mohammad Reza Atighi

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Tail Length ◽

Molecular Data ◽

Rrna Genes ◽

Valid Species ◽

Female Tail ◽

The Status ◽

Species Being ◽

Male Genital

Diploscapteroides persicus n. sp. is described and illustrated based on morphological, morphometric and molecular data. The new species is mainly characterised by female body length of 469-673 μm, female tail length of 51-99 μm, shape of gubernaculum and spicules and arrangement of male genital papillae. Also, the arrangement of male genital papillae of the new species shows the presence of precloacal papillae, a feature that has not been previously described for the genus. The new species is comparable with all valid species of the genus, namely D. boettgeri, D. brevicauda, D. chitinolabiatus, D. coroniger, D. dacchensis and D. flexuosus, from which the morphological comparisons of the new species with aforementioned species are discussed. Phylogenetic analyses using partial sequences of SSU and LSU rRNA genes were performed for the new species, being the first representative of the genus to be phylogenetically studied using both Bayesian inference (BI) and maximum likelihood (ML) methods, and revealed that D. persicus n. sp. formed a clade with a species of Cephaloboides.

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Species Delineation Within the Euwallacea fornicatus (Coleoptera: Curculionidae) Complex Revealed by Morphometric and Phylogenetic Analyses

Insect Systematics and Diversity ◽

10.1093/isd/ixy018 ◽

2018 ◽

Vol 2 (6) ◽

Cited By ~ 17

Author(s):

Demian F Gomez ◽

James Skelton ◽

M Sedonia Steininger ◽

Richard Stouthamer ◽

Paul Rugman-Jones ◽

...

Keyword(s):

Species Complex ◽

Phylogenetic Analyses ◽

Integrated Approach ◽

Type Specimen ◽

Morphological Characters ◽

Morphological Evidence ◽

Pest Species ◽

Species Delineation ◽

Genetic Lineages ◽

C Subunit

Abstract The ambrosia beetle Euwallacea fornicatus Eichhoff sensu lato is a complex of genetically divergent emerging pests responsible for damages to tree industries and ecosystems around the world. All lineages within the species complex are currently considered morphologically identical, presenting problems for their delineation and highlighting the shortcomings of species concepts based solely on type-specimen morphology. The objectives of this work were to 1) broaden the geographic sampling of the E. fornicatus complex in Asia, 2) reconstruct relationships between clades and populations, 3) find morphological characters or combinations of characters which are useful in delimiting the genetic lineages of the E. fornicatus species complex, and 4) propose taxonomic delimitation of species where morphology and phylogenetic identity correlate. Our integrated approach using molecular and morphological evidence suggests four clades that differ morphologically, but with overlap, therefore, cytochrome oxidase c subunit I (COI) barcoding remains necessary for assigning specimens to a clade. The following taxonomic changes are proposed: E. fornicatus (Eichhoff 1868) (= ‘Tea Shot Hole Borer Clade a’); E. fornicatior (Eggers 1923), stat. rev. (= ‘Tea Shot Hole Borer Clade b’); E. whitfordiodendrus (Schedl 1942), stat. rev. (= ‘Polyphagous Shot Hole Borer’); and E. kuroshio Gomez and Hulcr, sp. nov. (= ‘Kuroshio Shot Hole Borer’). This approach delivers a practical, evidence-based guidance for species delineation that can address overlapping variation in morphological characters of an emerging pest species complex.

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Total evidence phylogeny of the North American harvestman family Stygnopsidae (Opiliones : Laniatores : Grassatores) reveals hidden diversity

Invertebrate Systematics ◽

10.1071/is16053 ◽

2017 ◽

Vol 31 (3) ◽

pp. 317 ◽

Cited By ~ 4

Author(s):

Jesús A. Cruz-López ◽

Oscar F. Francke

Keyword(s):

Bayesian Inference ◽

Phylogenetic Analyses ◽

Mitochondrial Protein ◽

Morphological Characters ◽

Molecular Data ◽

Total Evidence ◽

The North ◽

Protein Encoding ◽

The Status ◽

Taxonomic Changes

Systematic relationships among Laniatores have received considerable attention during the past few years. Many significant taxonomic changes have been proposed, particularly in the superfamily Gonyleptoidea. As part of this superfamily, the basalmost Stygnopsidae is the least known family. In order to propose the first total evidence phylogeny of the family, we produced four datasets: three molecular markers – partial nuclear 28S, mitochondrial ribosomal 16S, mitochondrial protein-encoding cytochrome c oxidase subunit I; and 72 morphological characters. With these data, we performed three different phylogenetic analyses: (1) Bayesian Inference with molecular data, and (2) Bayesian Inference and (3) Maximum Likelihood using combined data. Our results are congruent: a monophyletic Stygnopsidae subdivided into two major clades: Stygnopsinae and Karosinae, subfam. nov. The following genera are redefined: Stygnopsis, Hoplobunus and Serrobunus stat. rev. The following taxa are described: Iztlina venefica, gen. nov., sp. nov. and Tonalteca, gen. nov. Additionally, the following changes are proposed: Serrobunus queretarius (Šilhavý, 1974), comb. nov., Stygnopsis apoalensis (Goodnight & Goodnight, 1973), comb. nov., Stygnopsis mexicana (Roewer, 1915), comb. nov., Stygnopsis oaxacensis (Goodnight & Goodnight, 1973), comb. nov., and Tonalteca spinooculorum (Goodnight & Goodnight, 1973), comb. nov. We also discuss the status of the genera Isaeus stat. rev. and Mexotroglinus. Finally, we discuss the evolution of male genitalia and convergence of selected homoplastic diagnostic characters.

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Molecular phylogenetic analyses and morphological variation point to taxonomic problems among four genera of parasitoid doryctine wasps (Hymenoptera : Braconidae)

Invertebrate Systematics ◽

10.1071/is14064 ◽

2015 ◽

Vol 29 (6) ◽

pp. 591 ◽

Cited By ~ 4

Author(s):

Marco Gebiola ◽

Antonio P. Garonna ◽

Umberto Bernardo ◽

Sergey A. Belokobylskij

Keyword(s):

Phylogenetic Analyses ◽

Morphological Characters ◽

Molecular Data ◽

Molecular Evidence ◽

A Posteriori ◽

Morphological Examination ◽

Ongoing Effort ◽

The Status ◽

Molecular Phylogenetic

Doryctinae (Hymenoptera : Braconidae) is a large and diverse subfamily of parasitic wasps that has received much attention recently, with new species and genera described and phylogenies based on morphological and/or molecular data that have improved higher-level classification and species delimitation. However, the status of several genera is still unresolved, if not controversial. Here we focus on two related groups of such genera, Dendrosoter Wesmael–Caenopachys Foerster and Ecphylus Foerster–Sycosoter Picard & Lichtenstein. We integrated morphological and molecular (COI and 28S–D2 genes) evidence to highlight, by phylogenetic analyses (maximum likelihood and Bayesian) and a posteriori morphological examination, previously overlooked variation, which is here illustrated and discussed. Monophyly of Dendrosoter and Caenopachys and the presence of synapomorphic morphological characters support synonymy of Caenopachys under Dendrosoter. Low genetic differentiation and high variability for putatively diagnostic morphological characters found in both C. hartigii (Ratzeburg) and C. caenopachoides (Ruschka) supports synonymy of D. caenopachoides under D. hartigii, syn. nov. Morphological and molecular evidence together also indicate independent generic status for Sycosoter, stat. rev., which is here resurrected. This work represents a further advancement in the framework of the ongoing effort to improve systematics and classification of the subfamily Doryctinae.

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Rejection of the monotypic status of Peromyscus furvus (Rodentia: Cricetidae), with consequences for its species group

Therya ◽

10.12933/therya-21-1122 ◽

2021 ◽

Vol 12 (2) ◽

pp. 347-367

Author(s):

Alejandro Cruz-Gómez ◽

A. Alondra Castro Campillo ◽

Zamira A. Ávila-Valle ◽

Livia León-Paniagua ◽

Marcia Ramírez-Sánchez ◽

...

Keyword(s):

Phylogenetic Analyses ◽

Mitochondrial Gene ◽

Species Group ◽

Probabilistic Methods ◽

Valid Species ◽

Single Type ◽

Morphometric Characters ◽

Size And Shape ◽

Skull Size ◽

Haplotype Networks

Previous studies using Cytochrome-b or ND3-ND4 mitochondrial gene have yielded intriguing evidence about the phylogenetic relationships among populations of Peromyscus furvus; however, those studies each based on phylogenies for a single type of genes, yielded conflicting topologies. In addition, analyses with traditional morphometrics have revealed differences in skull size among certain populations of the species. Therefore, in order to reassess the systematic and taxonomic status of P. furvus, we incorporated a suite of genetic and morphometric characters and employed cladistic analyses. Herein, we present results mostly derived from our genetic analyses (results from the phylogenetic examination of skull size and shape will appear later). Phylogenetic analyses were conducted using four mitochondrial genes (Cytb and ND3-ND4L-ND4) with the respective data analyzed separately or combined, followed by an analysis with genetic and morphometric data (size and shape characters). Most phylogenetic constructions were made with parsimonious methods, but probabilistic methods also were used in the analyses with the genes separated by type. Similar topologies were recovered from all analyses of the Cytb gene and from all parsimony analyses of the NADH genes; however, conflicting topologies were obtained with the probabilistic methods for the NADH genes. Additionally, to better understand the genetic variation in each type of gene, analyses for genetic divergence were conducted within and among genetic groups and haplotype networks were constructed. All the topologies obtained using genetic data questioned the monotypic status of P. furvus, as two additional clades were identified that seemingly correspond to unrecognized entities. The first of these, P. latirostris, occurs in the northern region and could be considered as either a species or subspecies. An unknown Peromyscus species nova that occurs to the south is considered as a valid species. Further, P. furvus s. s. becomes a polytypic species by recognizing at least two subspecies (P. f. angustirostris and P. f. furvus). Phylogenetic analyses also rejected membership of P. melanocarpus and P. ochraventer within the furvus species group. Instead, P. melanocarpus showed a greater affinity to P. mexicanus totontepecus, whereas, P. ochraventer either joined to the clade containing P. melanocarpus and P. m. tototepecus or to Megadontomys cryophilus in a sister clade. Finally, Osgoodomys banderanus (subgenus Haplomylomys) always remained basally positioned and segregated from all members of the subgenus Peromyscus.

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Hidden diversity in European bees: Andrena amieti sp. n., a new Alpine bee species related to Andrena bicolor (Fabricius, 1775) (Hymenoptera, Apoidea, Andrenidae)

Alpine Entomology ◽

10.3897/alpento.3.29675 ◽

2019 ◽

Vol 3 ◽

pp. 11-38 ◽

Cited By ~ 4

Author(s):

Christophe Praz ◽

Andreas Müller ◽

David Genoud

Keyword(s):

Phylogenetic Analyses ◽

Mitochondrial Gene ◽

Nuclear Gene ◽

World Heritage Site ◽

Swiss Alps ◽

Valid Species ◽

Morphological Examination ◽

Unesco World Heritage Site ◽

Alpine Species ◽

The Status

We revise the Alpine bee taxa related to Andrenabicolor (Fabricius, 1775), including A.montana Warncke, 1973 and A.allosa Warncke, 1975, the status of which has remained contentious. Phylogenetic analyses of one mitochondrial gene and one nuclear gene, as well as morphological examination reveal the presence of four Alpine species in this complex, one of which is new to science, A.amietisp. n. This new species is widely distributed in the Alps from southern France throughout Switzerland, northern Italy and southern Germany to Austria; a single record is known from the Apennines. The type locality is located within the Unesco World Heritage site “Swiss Alps Jungfrau-Aletsch”. Two widely divergent mitochondrial lineages are found in sympatry in A.amietisp. n.; the status of these lineages, which together form a paraphyletic unit from which A.allosa arose, is briefly discussed. We show that A.allosa, A.amietisp. n. and A.montana are polylectic but that each species exhibits a distinct spectrum of pollen hosts: the univoltine A.allosa shows affinities for pollen of the early-blooming Alpine plant genus Crocus. A.amietisp. n. is bivoltine and, as in A.bicolor, the summer generation exhibits a distinct preference for Campanulaceae, while the spring generation is widely polylectic. A.montana has a single generation in the summer and forages on a diversity of flowers such as Campanulaceae, Cistaceae and Caryophyllaceae. An identification key is presented for central European members of the subgenus Euandrena Hedicke, 1932. Lastly, the new Alpine species appears to represent the tip of the iceberg of substantial cryptic diversity in southern European Andrena (Euandrena): A.croatica Friese, 1887 is resurrected from synonymy with A.bicolor and treated as a valid species (stat. rev.), A.pileata Warncke, 1875, described as a subspecies of A.allosa, is elevated to species rank (stat. n.), and three additional unclear taxa are briefly described.

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Across the benthic and pelagic realms: a species‐level phylogeny of Benthesicymidae (Crustacea:Decapoda)

Invertebrate Systematics ◽

10.1071/is21004 ◽

2021 ◽

Vol 35 (7) ◽

pp. 776

Author(s):

Alexander L. Vereshchaka ◽

Dmitry N. Kulagin ◽

Anastasiia A. Lunina

Keyword(s):

Phylogenetic Analyses ◽

Bottom Layer ◽

Morphological Characters ◽

Valid Species ◽

Phylogenetic Study ◽

Depth Range ◽

Sea Floor ◽

Higher Taxa ◽

Insight Into ◽

Rare Opportunity

Benthesicymidae is a monophyletic group of Decapoda adapted to a life on the sea-floor, in the near-bottom layer, in the bathy- and in the mesopelagic, within an impressive depth range from a few hundred metres (Gennadas) to several thousand metres (Benthesicymus). Higher taxa are known to conquer all main oceanic biotopes such as the benthic, benthopelagic, and pelagic and a wide depth range but few family-level groups have clades evolved within all these oceanic realms. Therefore, the global fauna of Benthesicymidae provides a rare opportunity for an insight into phylogenetic processes favouring colonisation of all principal oceanic biotopes. The first comprehensive phylogenetic study of Benthesicymidae (all 37 valid species) is based on six molecular markers and 105 morphological characters (including 72 female and male copulatory characters). Analyses resulted in trees with similar topology and the same set of robust clades. Molecular methods based on 167 sequences (84 new) provided better resolution of deeper nodes and generally higher support of the clades, while morphological methods allowed analyses of all valid species of the global fauna. Phylogenetic analyses support the monophyly and robustness of all currently known genera except Gennadas, which was split into Gennadas Bate, 1881, Amalopenaeus Smith, 1882, and Notogennema gen. nov. We also retrieved two major clades for which we erected two new subfamilies: Benthesicyminae subfam. nov. (presumably benthic, genera Altelatipes, Bathicaris, Benthesicymus, and Benthonectes) and Gennadinae subfam. nov. (presumably pelagic, genera Amalopenaeus, Bentheogennema, Benthoecetes, Boreogennema, Gennadas, Maorrancaris, and Notogennema gen. nov.). We revealed two groups of morphological characters, that are interlinked evolutionarily: (1) petasma and thelycum; (2) body, mouthparts, and pereopods. Morphological traits within benthic and pelagic clades are different, a model explaining the differences is proposed. Along with previous studies, our results confirm the idea that the elaboration of the copulatory structures is a key to successful colonisation of the pelagic realm. These results extend our knowledge about evolution in the largest habitual biotope of our planet and phylogenetic processes favouring colonisation of all principal oceanic biotopes.

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Resurrection of Crypturgus subcribrosus Eggers 1933 stat. n., and its close phylogenetic relationship to Nearctic Crypturgus (Coleoptera, Scolytinae)

Zootaxa ◽

10.11646/zootaxa.1606.1.3 ◽

2007 ◽

Vol 1606 (1) ◽

pp. 41-50 ◽

Cited By ~ 4

Author(s):

BJARTE H. JORDAL ◽

MILOŠ KNÍšEK

Keyword(s):

Sequence Data ◽

Phylogenetic Analyses ◽

Elongation Factor ◽

Morphological Characters ◽

Valid Species ◽

Dna Barcodes ◽

Elongation Factor 1Α ◽

Sister Relationship ◽

Dna Sequence Data ◽

Close Phylogenetic Relationship

Crypturgus subcribrosus Eggers is removed from synonymy under C. cinereus (Herbst) and reinstated as a valid species based on evidence from DNA sequence data and morphological features. Phylogenetic analyses of Elongation Factor 1α and Cytochrome Oxidase I sequences in conjunction with morphological characters revealed a sister relationship between C. subcribrosus and two Nearctic species of Crypturgus, with C. cinereus unrelated to any of these taxa. Type material of C. cinereus has been located and lectotype with paralectotypes are designated. Amended diagnoses that include DNA barcodes are presented for C. subcribrosus and C. cinereus together with an identification key to the Fennoscandian species of Crypturgus.

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A molecular phylogeny of Porcellionidae (Isopoda, Oniscidea) reveals inconsistencies with present taxonomy

ZooKeys ◽

10.3897/zookeys.801.23566 ◽

2018 ◽

Vol 801 ◽

pp. 163-176 ◽

Cited By ~ 3

Author(s):

Andreas C. Dimitriou ◽

Stefano Taiti ◽

Helmut Schmalfuss ◽

Spyros Sfenthourakis

Keyword(s):

Phylogenetic Analyses ◽

Morphological Characters ◽

Morphological Evidence ◽

Molecular Evidence ◽

Geographical Pattern ◽

The Family ◽

The Status ◽

Tree Topologies ◽

Globally Distributed ◽

Basal Position

Porcellionidae is one of the richest families of Oniscidea globally distributed but we still lack a comprehensive and robust phylogeny of the taxa that are assigned to it. Employing five genetic markers (two mitochondrial and three nuclear) we inferred phylogenetic relationships among the majority of Porcellionidae genera. Phylogenetic analyses conducted via Maximum Likelihood and Bayesian Inference resulted in similar tree topologies. The mtDNA genes cytochrome oxidase I (COI) and 16s rRNA (16s) were used for clade dating using previously published mutation rates. Our results provide evidence against the monophyly of both Porcellionidae and the largest genus of the family Porcellio. These results are compared to previous published work based on morphological evidence. The genera Leptotrichus and Brevurus are not grouped with the rest of Porcellionidae whereas Agnaridae are grouped with part of Porcellionidae. Armadillidium and Schizidium (Armadillidiidae) occupy a basal position on the phylogenetic tree. Even though the African genera Tura and Uramba (distributed in East Africa) are grouped together there is no general geographical pattern in other sub-clades. Additional taxonomic issues that arise in this work such as the assignment of the recently described genus Levantoniscus, arealso discussed. The status of Porcellionidae should be further revised and morphological characters traditionally used in Oniscidea taxonomy should be reconsidered in view of molecular evidence. The origin of the monophyletic clade within Porcellionidae as indicated in the present work is dated back to the Oligocene (~32 mya).

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