scholarly journals Clindamycin Efficacy in Patients With Methicillin-Sensitive Staphylococcus aureus in a Fourth-Level Hospital in the City of Medellín

Cureus ◽  
2022 ◽  
Author(s):  
Jose C Alvarez-Payares ◽  
Jair E. Palacios M ◽  
Edwin De la Peña ◽  
Huxdley B. Cabrera ◽  
Santiago Giraldo-Ramírez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Fourth Level ◽  
The City ◽  
Level Hospital

scholarly journals Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm

Toxins ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 14
Author(s):  
Thamiris Santana Machado ◽  
Felipe Ramos Pinheiro ◽  
Lialyz Soares Pereira Andre ◽  
Renata Freire Alves Pereira ◽  
Reginaldo Fernandes Correa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Protein A ◽  
Invasive Infection ◽  
Type I ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Spa Gene ◽  
The City

Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niterói—Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal chromosomal cassette (SCCmec), mecA, Panton-Valentine Leucocidin (PVL), icaC, icaR, ACME, and hla virulence genes. The strength of biofilm formation has also been exploited. The prevalence of SCCmec type IV (77.1%) was observed in the colonization group; however, in the invasive infection group, SCCmec type II was prevalent (62.9%). The Multilocus Sequence Typing (MLST), ST5/ST30, and ST5/ST239 analyses were the most frequent clones in colonization, and invasive infection isolates, respectively. Among the isolates selected to assess the ability to form a biofilm, 51.06% were classified as strong biofilm builders. Surprisingly, we observed that isolates other than the Brazilian Epidemic Clone (BEC) have appeared in Brazilian hospitals. The virulence profile has changed among these isolates since the ACME type I and II genes were also identified in this collection.

scholarly journals Detection of β-lactamase, blaZ and mecA in penicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Garanhuns, Brazil

2021 ◽  
Vol 15 (2) ◽  
pp. 140-145
Author(s):  
Amanda Pereira Lucas ◽  
Andriele Renata Barbosa de Farias ◽  
Elizabete Cristina da Silva ◽  
Kleber Régis Santoro ◽  
Marcelo Mendonça ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Meca Gene ◽  
Resistance Mechanism ◽  
Bovine Mastitis ◽  
Duplex Pcr ◽  
Genetic Determinants ◽  
The City

There are few reports in the literature about genetic determinants of resistance to β-lactams in Staphylococcus aureusisolated from dairy cattle located in the municipality of Garanhuns, state of Pernambuco, Brazil. Thus, this study aimed to investigate the production of β-lactamase and the presence of the blaZ and mecA genes in penicillin-resistant S. aureus isolated from cases of subclinical bovine mastitis in the city of Garanhuns. Forty-six strains of penicillin-resistant S. aureus were evalu-ated using the nitrocefin disc test and duplex PCR. The results revealed that 45 strains (97.8%) were positive for β-lactamase production and 44 (95.7%) carried the blaZ gene. Among the latter, 43 (97.7%) were β-lactamase producers and only one (2.3%) was not. The mecA gene was not detected in any of the isolates investigated. The results suggest that enzymatic inacti-vation is the main β-lactam resistance mechanism expressed by S. aureus in the herds analyzed.

scholarly journals Análise Microbiológica de Batons de Uso Compartilhado na Cidade de Juazeiro do Norte-CE

2021 ◽  
Vol 24 (5-esp.) ◽  
pp. 636-639
Author(s):  
Luana Romão Rodrigues ◽  
João Victor de Freitas Menezes ◽  
Dárcio Luiz de Sousa Júnior ◽  
Rafael de Carvalho Mendes
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Scientific Work ◽  
Quality Standard ◽  
Microbiological Analysis ◽  
Great Circulation ◽  
Effective Use ◽  
The City

A cosmetologia é a ciência que engloba e estuda os cosméticos, desde conceitos iniciais até a sua fabricação, tornando-se uma ciência multidisciplinar. Para garantia de um uso seguro e eficaz dos cosméticos é necessário a realização de análises para se conseguir um padrão de qualidade. Quando há indícios de contaminação por microrganismos no cosmético, diversos problemas podem ser encontrados, como a ocorrência de patologias específicas, causando altos riscos e complicações à saúde do usuário. O objetivo deste trabalho foi investigar qualitativamente através de análises microbiológicas a qualidade dos produtos cosméticos de maquiagem usados de forma compartilhada, utilizando amostras colhidas aleatoriamente em uma loja de varejo, que foi selecionada por ser de grande circulação, da cidade de Juazeiro do Norte, Ceará. Nesta pesquisa foram analisadas oito amostras coletadas durante o período de outubro e novembro de 2017. As análises que foram realizadas seguiram a Farmacopeia brasileira. Como resultado obteve-se um percentual de 87,5 % de contaminação microbiana, evidenciando a presença de Staphylococcus aureus, Escherichia coli, Enterococcus sp. e Pseudomonas aeruginosa, sendo que apenas uma das oito amostras analisadas mostrou-se adequada para uso seguindo os limites de aceitação microbiológicas permitidos por lei. Baseados nesses resultados foi possível concluir que o índice de contaminação das amostras de batons que são utilizadas de modo compartilhado são potencialmente maiores. Com isso, intensifica-se a necessidade de medidas que impeçam o compartilhamento dos produtos cosméticos, com a finalidade de diminuir o risco de uma infecção coletiva.   Palavras-chave: Cosméticos. Uso compartilhado. Contaminação.   Abstract Cosmetology is the science that encompasses and studies cosmetics, from initial concepts to their manufacture, becoming a multidisciplinary science. To guarantee a safe and effective use of cosmetics it is necessary to carry out analyzes to achieve a quality standard. When there is evidence of contamination by microorganisms in the cosmetic, several problems can be found, such as the occurrence of specific pathologies, causing high risks and complications to the health of the user. The objective of this scientific work was to qualitatively investigate through microbiological analysis the quality of cosmetic makeup products used in a shared way, using samples collected randomly in a retail store, which was selected because it is of great circulation, in the city of Juazeiro do Norte, Ceará. In this research, eight (8) samples collected during the period of October and November 2017 were analyzed. The analyzes that were carried out followed the Brazilian Pharmacopoeia. As a result, a percentage of 87.5 % of microbial contamination was obtained, showing the presence of Staphylococcus aureus, Escherichia coli, Enterococcus sp. and Pseudomonas aeruginosa, with only one of the eight samples analyzed being suitable for use following the limits of microbiological acceptance allowed by law. Based on these results it was possible to conclude that the contamination index of the lipstick samples that are used in a shared way are potentially higher. As a result, the need for measures to prevent the sharing of cosmetic products is intensified, in order to reduce the risk of a collective infection.   Keywords: Cosmetics. Shared Use. Contamination.

scholarly journals Comparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, Colombia

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Andrés Ceballos-Garzón ◽  
Gloria Cortes ◽  
Florent Morio ◽  
Edna L. Zamora-Cruz ◽  
Melva Y. Linares ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Fourth Level ◽  
Level Hospital ◽  
Tof Ms

Microbiological and parasitological quality of ground beef sold in Aracaju-SE

2015 ◽  
Vol 6 (2) ◽  
pp. 15
Author(s):  
Sydney Correia Leao ◽  
Debora Machado Barreto ◽  
Viviane Da Costa Ribeiro ◽  
Roneval Felix De Santana ◽  
Cláudia Moura De Melo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Ascaris Lumbricoides ◽  
Ground Beef ◽  
Fecal Coliforms ◽  
Salmonella Spp ◽  
Class A ◽  
Low Levels ◽  
The City ◽  
Butcher Shops

<p><strong><em>The goal of this work was to verify the presence of microorganisms and parasites in the ground beef sold in the city of Aracaju, Sergipe, in addition to compare the contamination in butcher shops and supermarkets in neighborhoods Class A and D. We collected 16 samples of 100 gram in triplicate (m=48) and analyzed the bacteria Staphylococcus aureus, Salmonella spp. coliforms total and thermotolerants, molds and yeasts, and parasites. It was observed that the samples are contaminated by these microorganisms examined with high total and fecal coliforms, Salmonella spp. (25%), low levels of S. aureus, molds and yeasts, in addition to the detection of Ascaris lumbricoides, larvae of flies, mites and plant artifacts.</em></strong></p>

Microbiological Quality of Spices Marketed in the City of Botucatu, São Paulo, Brazil

2009 ◽  
Vol 72 (2) ◽  
pp. 421-424 ◽  
Author(s):  
P. L. MOREIRA ◽  
T. B. LOURENÇÃO ◽  
J. P. A. N. PINTO ◽  
V. L. M. RALL
Keyword(s):  
Staphylococcus Aureus ◽  
Primary Production ◽  
Bacillus Cereus ◽  
Microbiological Quality ◽  
Black Pepper ◽  
Sao Paulo ◽  
São Paulo ◽  
Thermotolerant Coliforms ◽  
The City

The aim of this work was to assess the microbiological quality of spices sold in Botucatu, São Paulo, Brazil. A total of 233 samples were analyzed for mesophilic bacteria, thermotolerant coliforms, Bacillus cereus, Staphylococcus aureus, and Salmonella. Data showed that 21 and 5.6% of these samples were not in agreement with the standards of Brazilian law, due to an excess of coliforms and to the presence of Salmonella, respectively. Black pepper and cumin exhibited the lowest microbiological quality, whereas bay leaf showed the highest quality. It was concluded that the seasonings possessed poor microbiological quality, and new alternatives should be taken in the primary production in order to improve this quality. Irradiation may also be a tool to assure the safety of these products.

scholarly journals Characterization ofStaphylococcus aureusIsolates That Colonize Medical Students in a Hospital of the City of Cali, Colombia

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Luis Fernando Collazos Marín ◽  
Gina Estupiñan Arciniegas ◽  
Monica Chavez Vivas
Keyword(s):  
Staphylococcus Aureus ◽  
Medical Students ◽  
Pcr Amplification ◽  
Nasal Carriage ◽  
Clinical Rotation ◽  
Carriage Rate ◽  
Methicillin Resistant ◽  
Hospital Environments ◽  
The City

Introduction. Nasal carriage of methicillin-resistantStaphylococcus aureus(MRSA) represents a risk for the spread of bacteria. This study characterized theS. aureusisolated from medical students, who were in their clinical rotation at a hospital in the city of Cali.Materials and Methods. 216 students participated in the study and 63 isolates ofS. aureuswere evaluated for susceptibility and PCR amplification ofagrandmecA genes. The origin of MRSA isolates was established by analyzingagrpolymorphisms.Results. A total of 29.2% of students were colonized byS. aureusand nasal carriage rate was 23.6% and 14.3% MRSA. Threeagrgroups (agrII, andagrIII) were identified; theagrI group was the most common, with a 35% prevalence; this group is from community origin.Conclusion. The present study demonstrates that medical students carryS. aureusstrains, with the threat of spreading them both to community and hospital environments.

Carriage of Methicillin-resistant Staphylococcus aureus among people living with HIV-AIDS in inner São Paulo State, Brazil: molecular and spatial epidemiology

2020 ◽  
Author(s):  
Leticia Chamma Lastória ◽  
Maria de Lourdes Ribeiro de Souza da Cunha ◽  
Camila Sena Martins de Souza ◽  
Lenice do Rosário de Souza ◽  
Cassiano Victória ◽  
...  
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Spatial Analysis ◽  
Spatial Epidemiology ◽  
People Living With Hiv ◽  
Methicillin Resistant ◽  
Paulo State ◽  
Living With Hiv ◽  
Mrsa Colonization ◽  
The City

Abstract Background: Methicillin-resistant Staphylococcus aureus (MRSA) is increasingly recognized as a threat for people living with HIV/AIDS (PLWHA). However, the magnitude of asymptomatic MRSA colonization in that group varies among different countries and geographic regions. Methods: We conducted a study that aimed at identifying the prevalence, risk factors and spatial epidemiology of both overall S. aureus and MRSA colonization among PLWHA from small cities from inner São Paulo State, Brazil. MRSA isolates were characterized using Pulsed-Field Gel Electrophoresis (PFGE), and submitted to typing of the Staphylococcal Chromosome Cassete (SCC)mec. Spatial analysis was performed to search for geographical clusters and correlation with socioeconomic indicators. Results: In a first point prevalence survey, nasal and oropharyngeal swabs of 368 people were collected. Sixty-seven subjects from the city of Botucatu were surveyed for colonization in two other occasions, and had swabs collected from household members. The prevalence rates for S. aureus and MRSA in the first survey were 25.8% and 2.7%. The overall S. aureus colonization was negatively associated with the use of beta-lactams and of illicit drugs. On the other hand, MRSA colonized subjects were more likely to use crack and to have been admitted to a hospital during the past year. Repeated surveys found additional cases of MRSA colonization, but all subjects were positive in only one occasion. Four PFGE clusters were characterized, grouping subjects in household, city and region level. Of 19 total MRSA isolates, only one did not harbor SCCmec type IV. Spatial analysis of households of subjects living in the city of Botucatu found significant overdispersion of cases, but no association with socio-economic indicators. Conclusion: We found small but relevant prevalence of MRSA among PLWHA. Community and healthcare-associated risk factors were identified, so that predominant routes of transmission could not be determined on epidemiological grounds.

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