scholarly journals ANALYSIS OF ESSENTIAL OIL CONSTITUENTS OF THREE ANNONA SPECIES GROWING IN KERALA, INDIA

2021 ◽  
Vol 12 (5) ◽  
pp. 45-48
Author(s):  
Sonia Mol Joseph ◽  
Amala Dev A R

The volatile chemical composition of leaf essential oils of three Annona species (Annona cherimola, Annona muricata and Annona squamosa) from the different regions of Kerala, South Indian was determined using gas chromatography-mass spectrometry analysis. A total of 41 constituents belonging to monoterpenoids, sesquiterpenoids and diterpenoids were identified. Monoterpenes and sesquiterpenoids were the major class of volatile compounds in most of the Annona species examined. The study led to the identification of major compounds as germacrene D (23.5%), bicyclogermacrene (14.6%) and β-caryophyllene (11.7%) in A. cherimola, α-pinene (13.3%), β-caryophyllene (11.2%) and β-pinene (10.1%) in A. muricata, and β-caryophyllene (11.9%) and α-pinene (8.2%) in A. squamosa respectively. The examined essential oils showed that β-caryophyllene is the common constituent identified in all the three species and other constituents are more specific for each species under study. Present investigation reports, a comparison of essential oil compositions of three Annona species from Kerala. Results of this study prove that essential oils examined have considerable dissimilarity in chemical composition with previously reported leaf essential oil compositions from other regions. A chemotaxonomic analysis of these essential oils based on the distribution of compounds has revealed an efficient method to differentiate Annona species unambiguously.

Plants ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 79 ◽  
Author(s):  
María Ibáñez ◽  
María Blázquez

The chemical composition of winter savory, peppermint, and anise essential oils, and in vitro and in vivo phytotoxic activity against weeds (Portulaca oleracea, Lolium multiflorum, and Echinochloa crus-galli) and food crops (maize, rice, and tomato), have been studied. Sixty-four compounds accounting for between 97.67–99.66% of the total essential oils were identified by Gas Chromatography-Mass Spectrometry analysis. Winter savory with carvacrol (43.34%) and thymol (23.20%) as the main compounds produced a total inhibitory effect against the seed germination of tested weed. Menthol (48.23%), menthone (23.33%), and iso-menthone (16.33%) from peppermint only showed total seed germination inhibition on L. multiflorum, whereas no significant effects were observed with trans-anethole (99.46%) from anise at all concentrations (0.125–1 µL/mL). Low doses of peppermint essential oil could be used as a sustainable alternative to synthetic agrochemicals to control L. multiflorum. The results corroborate that in vivo assays with a commercial emulsifiable concentrate need higher doses of the essential oils to reproduce previous in vitro trials. The higher in vivo phytotoxicity of winter savory essential oil constitutes an eco-friendly and less pernicious alternative to weed control. It is possible to achieve a greater in vivo phytotoxicity if less active essential oil like peppermint is included with other active excipients.


2018 ◽  
Vol 16 (1) ◽  
pp. 317-323 ◽  
Author(s):  
Tomasz Baj ◽  
Izabela Korona-Głowniak ◽  
Radosław Kowalski ◽  
Anna Malm

AbstractHyssopus officinalis L. is a common plant that is most usually found in three color forms - f. cyaneus (blue), f. ruber Mill. (purple/pink) and f. albus Alef (white). In the present work, we evaluated the chemical composition and antimicrobial activity of essential oils obtained from Polish-sourced white- and pink-flowered H. officinalis. Gas chromatography-mass spectrometry analysis of the essential oil has shown that both forms of color have a different content of main components. The principle essential oil component of white-flowered H. officinalis L. was pinocamphone (51%), while pink-flowered H. officinalis L. contained almost equal amounts of pinocamphone (28.8%) and isopinocamphone (21.9%). Of note, the essential oil of the pink form was more active against Grampositive bacteria, especially against Bacillus subtilis.


2020 ◽  
Vol 75 (11-12) ◽  
pp. 473-478
Author(s):  
Natasa Mohd Shakri ◽  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Shamsul Khamis ◽  
Nor Azah Mohamad Ali ◽  
Shazlyn Milleana Shaharudin

AbstractPolyalthia is one of the largest genera in the Annonaceae family, and has been widely used in folk medicine for the treatment of rheumatic fever, gastrointestinal ulcer, and generalized body pain. The present investigation reports on the extraction by hydrodistillation and the composition of the essential oils of four Polyalthia species (P. sumatrana, P. stenopetalla, P. cauliflora, and P. rumphii) growing in Malaysia. The chemical composition of these essential oils was determined by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The multivariate analysis was determined using principal component analysis (PCA) and hierarchical clustering analysis (HCA) methods. The results revealed that the studied essential oils are made up principally of bicyclogermacrene (18.8%), cis-calamenene (14.6%) and β-elemene (11.9%) for P. sumatrana; α-cadinol (13.0%) and δ-cadinene (10.2%) for P. stenopetalla; δ-elemene (38.1%) and β-cubebene (33.1%) for P. cauliflora; and finally germacrene D (33.3%) and bicyclogermacrene for P. rumphii. PCA score and HCA plots revealed that the essential oils were classified into three separated clusters of P. cauliflora (Cluster I), P. sumatrana (Cluster II), and P. stenopetalla, and P. rumphii (Cluster III) based on their characteristic chemical compositions. Our findings demonstrate that the essential oil could be useful for the characterization, pharmaceutical, and therapeutic applications of Polyalthia essential oil.


2010 ◽  
Vol 113-116 ◽  
pp. 1644-1647 ◽  
Author(s):  
Zhi Ming Liu ◽  
Hai Ying Wang ◽  
Shan Shan Liu ◽  
Nai Xiang Jiang

The volatile components of essential oils of fresh aerial parts from horseweed (Conyza canadensis) collected in October with hydrodistillation and steam distillation, respectively, were analyzed by gas chromatography-mass spectrometry. The results showed that limonene, β-pinene, trans-α-bergamotene, cis-β-ocimene, β-myrcene, germacrene D, 1,3,8-p-menthatriene, caryophyllene, (E)-β-farnesene, (Z)-β-farnesene, α-curcumene, caryophyllene oxide were the common volatile components in the essential oils between hydrodistillation and steam distillation. High limonene content (68.87%) was in the essential oil with hydrodistillation. Limonene or other terpenoids has the potential to develop as the biomimetic synthesis template for environmentally-friendly herbicide.


Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1829
Author(s):  
Alexandru Ciocarlan ◽  
Lucian Lupascu ◽  
Aculina Aricu ◽  
Ion Dragalin ◽  
Violeta Popescu ◽  
...  

The producers of essential oils from the Republic of Moldova care about the quality of their products and at the same time, try to capitalize on the waste from processing. The purpose of the present study was to analyze the chemical composition of lavender (Lavanda angustifolia L.) essential oil and some by-products derived from its production (residual water, residual herbs), as well as to assess their “in vitro” antimicrobial activity. The gas chromatography-mass spectrometry analysis of essential oils produced by seven industrial manufacturers led to the identification of 41 constituents that meant 96.80–99.79% of the total. The main constituents are monoterpenes (84.08–92.55%), followed by sesquiterpenes (3.30–13.45%), and some aliphatic compounds (1.42–3.90%). The high-performance liquid chromatography analysis allowed the quantification of known triterpenes, ursolic, and oleanolic acids, in freshly dried lavender plants and in the residual by-products after hydrodistillation of the essential oil. The lavender essential oil showed good antibacterial activity against Bacillus subtilis, Pseudomonas fluorescens, Xanthomonas campestris, Erwinia carotovora at 300 μg/mL concentration, and Erwinia amylovora, Candida utilis at 150 μg/mL concentration, respectively. Lavender plant material but also the residual water and ethanolic extracts from the solid waste residue showed high antimicrobial activity against Aspergillus niger, Alternaria alternata, Penicillium chrysogenum, Bacillus sp., and Pseudomonas aeroginosa strains, at 0.75–6.0 μg/mL, 0.08–0.125 μg/mL, and 0.05–4.0 μg/mL, respectively.


2018 ◽  
Vol 16 (S1) ◽  
pp. S104-S108 ◽  
Author(s):  
M. Touaibia ◽  
F. Saidi ◽  
D. Abdellali ◽  
F.Z. Raber Elmaizi

Essential oil of Ammodaucus leucotrichus subsp. leucotrichus Coss & Dur (endemic), extracted from the seeds by hydrodistillation, was screened for its possible antimicrobial activity as well as its chemical composition. According to the Gas Chromatography/Mass Spectrometry analysis, 35 components were identified (98.23%), perillaldehyde (45.58%) and limonene (14.02%) being the major components. The antimicrobial test of the essential oil showed that Staphylococcus aureus (MIC = 4.5 mg/ml) and Bacillus cereus (MIC = 9 mg/ml) were the most sensitive strains, followed by Candida krusei and Candida albicans (MIC = 18 mg/ml both).


2016 ◽  
Vol 46 (5) ◽  
pp. 937-942 ◽  
Author(s):  
Marcelo Nogueira Xavier ◽  
Cássia Cristina Fernandes Alves ◽  
Cristiane de Melo Cazal ◽  
Nathalia Horrana Santos

ABSTRACT: The Annonaceae family consists of ~135 genera with diverse species and a large number of chemical compounds arising from the secondary metabolism. However, the chemical composition of the essential oil of several species of the family such as Cardiopetalum calophyllum has not been completely determined. In this study, the essential oils extracted from the leaves, flowers, and fruits of C. calophyllum , collected in typical areas of the Cerrado of Goiás, were characterized. The essential oil was extracted by hydrodistillation for 4h using a Clevenger apparatus. The chemical analysis was carried out by gas chromatography-mass spectrometry (GC-MS), and the constituents were identified by comparing the spectra and retention index obtained with those available in the literature. Twenty three compounds were identified in the essential oil of leaves; the oxygenated sesquiterpene, spathulenol (28.78%), was the major compound. Twenty five compounds were identified in the flowers, mainly comprising sesquiterpene hydrocarbons, germacrene-D (37.03%) and germacrene-B (13.72%). Seventeen compounds were reported in fruits, mainly comprising sesquiterpene hydrocarbons, germacrene-D (28.19%) and germacrene-B (20.90%), and an oxygenated sesquiterpene, spathulenol (11.53%). This is the first report on the chemical composition of the essential oils from leaves, flowers, and fruits of C. calophyllum.


2020 ◽  
Vol 10 ◽  
Author(s):  
Ionara I. Dalcol ◽  
Alessandra O. Pereira ◽  
Luisa H. Paz ◽  
Gabriela Benetti ◽  
Fallon S. Siqueira ◽  
...  

Background: Aristolochia triangularis Cham., popularly known as the "cipó-mil-homens", "angelicó" and "ypê-mi", is applied for the treatment of wounds, skin diseases (6,7), digestive and circulatory system diseases as an antipyretic and for malaria fever. Objective: In this work we investigated the chemical composition, the antimicrobial and antimycobacterial activities of the essential oils (EOs) extracted from A. triangularis fresh stems and leaves collected in Southern Brazil. Methods: Fresh stems and fresh leaves of Aristolochia triangularis Cham. were separately subjected to hydrodistillation using a Clevenger-type apparatus. The chemical composition of the essential oils (EOs) were analyzed by gas chromatography/mass spectrometry (GC/MS). The oil samples were evaluated for their antimycobacterial, antibacterial and antifungal activities against twenty-four microorganisms. Results: Hydrodistillation of fresh stems and leaves of A. triangularis resulted in 0.16% (w/w) and 0.37% (w/w) respectively of light-yellow oils. Germacrene D is found in 13.2 - 13.5% in both EOs. The constituent most abundant in the stems EO (19.18%) was the oxygenated diterpene ent-Kaur-16-en-19-al (10), along with E-nerolidol (17.89%). The main constituents of the leaves EO were bicyclogermacrene (24.79%), β-elemene (11.30%), E-caryophyllene (10.40%) and germacrene A (9.42%), in addition to the previously mentioned germacrene D. The stems and leaves EOs showed capacity to inhibit the Gram-negative Enterobacter aerogenes and the stems EO capacity to inhibit Staphylococcus aureus, with MIC values of 31.2 µg/mL. S. aureus was moderately sensitive to leaves EO, while stems EO displayed moderate activity against Enterococcus faecalis and Salmonella typhimurium (MIC values of 62.5 µg/mL). Candida glabrata was highly susceptible to both EOs (MIC values < 3.9 µg/mL). The EOs showed moderate potential to inhibit the growth of Cryptococcus gatti and Cryptococcus neoformans (MICs of 62.5 μg/mL). Conclusion: The A. triangularis essential oils from stems and leaves displayed capacity to inhibit Enterobacter aerogenes (MIC values of 31.2 µg/mL) and high antifungal effect against Candida glabrata (MIC values of <3.9 µg/mL). Mycobacterium massiliense and M. abscessus were susceptible to the leaves EO, with MICs of 39.06 μg/mL. These results showed the A. triangularis essential oils potential as antifungal and antimycobacterial to be used in the development of new antibiotic.


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