Relationship between Testicular Volume and Sperm Count in Infertile Men in Southern Nigeria

2017 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
B Manuel ◽  
E Ugboma ◽  
N Nwankwo
Keyword(s):  
Sperm Count ◽  
Testicular Volume ◽  
Infertile Men ◽  
Southern Nigeria

scholarly journals FSHB Genotype Identified as a Relevant Diagnostic Parameter Revealed by Cluster Analysis of Men With Idiopathic Infertility

2021 ◽  
Vol 12 ◽  
Author(s):  
Henrike Krenz ◽  
Andrea Sansone ◽  
Sabine Kliesch ◽  
Joerg Gromoll ◽  
Maria Schubert
Keyword(s):  
Sperm Count ◽  
Testicular Volume ◽  
Entire Study ◽  
Idiopathic Infertility ◽  
Partitioning Around Medoids ◽  
Infertile Men ◽  
Heterogenous Group ◽  
Genetic Abnormalities ◽  
Genotype Cluster ◽  
Study Population

Introduction and ObjectivesAbout 30-75% of infertile men are diagnosed with idiopathic infertility, thereby lacking major causative factors to explain their impaired fertility status. In this study, we used a large cohort of idiopathic infertile men to determine whether subgroups could be identified by an unbiased clustering approach and whether underlying etiologic factors could be delineated.Patients and MethodsFrom our in-house database Androbase®, we retrospectively selected patients (from 2008 to 2018) with idiopathic male infertility (azoo- to normozoospermia) who fit the following selection criteria: FSH ≥ 1 IU/l, testosterone ≥ 8 nmol/l, ejaculate volume ≥ 1.5 ml. Patients with genetic abnormalities or partners with female factors were excluded.For the identified study population (n=2742), we used common andrologic features (somatic, semen and hormonal parameters, including the FSHB c.-211G>T (rs10835638) single nucleotide polymorphism) for subsequent analyses. Cluster analyses were performed for the entire study population and for two sub-cohorts, which were separated by total sperm count (TSC) thresholds: Cohort A (TSC ≥ 1 mill/ejac; n=2422) and Cohort B (TSC < 1 mill/ejac; n=320). For clustering, the partitioning around medoids method was employed, and the quality was evaluated by average silhouette width.ResultsThe applied cluster approach for the whole study population yielded two separate clusters, which showed significantly different distributions in bi-testicular volume, FSH and FSHB genotype. Cluster 1 contained all men homozygous for G (wildtype) in FSHB c.-211G>T (100%), while Cluster 2 contained most patients carrying a T allele (>96.6%). In the analyses of sub-cohorts A/B, two clusters each were formed too. Again, the strongest segregation markers between the respective clusters were bi-testicular volume, FSH and FSHB c.-211G>T.ConclusionWith this first unbiased approach for revealing putative subgroups within a heterogenous group of idiopathic infertile men, we did indeed identify distinct patient clusters. Surprisingly, across all diverse phenotypes of infertility, the strongest segregation markers were FSHB c.-211G>T, FSH, and bi-testicular volume. Further, Cohorts A and B were significantly separated by FSHB genotype (wildtype vs. T-allele carriers), which supports the notion of a contributing genetic factor. Consequently, FSHB genotyping should be implemented as diagnostic routine in patients with idiopathic infertility.

scholarly journals Is there a place for nutritional supplements in the treatment of idiopathic male infertility?

2014 ◽  
Vol 86 (3) ◽  
pp. 164 ◽  
Author(s):  
Davide Arcaniolo ◽  
Vincenzo Favilla ◽  
Daniele Tiscione ◽  
Francesca Pisano ◽  
Giorgio Bozzini ◽  
...  
Keyword(s):  
Male Infertility ◽  
Sperm Count ◽  
Nutritional Supplements ◽  
Semen Parameters ◽  
Male Factor ◽  
Treatment Regimens ◽  
Idiopathic Male Infertility ◽  
Infertile Men ◽  
Wide Variability

Objective: Infertility affects 15% of couples in fertile age. Male factor is a cause of infertility in almost half of cases, mainly due to oligoasthenoteratozoospermia (OAT). The purpose of this study is to review the effects of nutritional supplements as medical treatment for idiopathic male infertility. Material and methods: A Pub Med and Medline review of the published studies utilizing nutritional supplements for the treatment of male infertility has been performed. Results: Clinical trials on Vitamin E, Vitamin A, Vitamin C. Arginine, Carnitine, N-Acetyl-Carnitine, Glutathione, Coenzyme Q10, Selenium and Zinc were reviewed. Although there is a wide variability in selected population, dose regimen and final outcomes, nutritional supplements both alone and in combination seems to be able to improve semen parameters (sperm count, sperm motility and morphology) and pregnancy rate in infertile men. Conclusions: There are rising evidences from published randomized trials and systematic review suggesting that nutritional supplementation may improve semen parameters and the likelihood of pregnancy in men affected by OAT. This improvement, however, is not consistent and there is a wide variation in the treatment regimens used. Well designed and adequately powered RCTs are needed to better clarify the role of nutritional supplements as treatment for male infertility.

O-151 Detailed characterization of infertile men with idiopathic versus unexplained infertility: a single-center experience

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
L Boeri ◽  
L Candela ◽  
E Pozzi ◽  
F Belladelli ◽  
P Capogrosso ◽  
...  
Keyword(s):  
Charlson Comorbidity Index ◽  
Semen Analysis ◽  
Unexplained Infertility ◽  
Testicular Volume ◽  
Multivariable Logistic Regression Analysis ◽  
Sperm Dna Fragmentation ◽  
Serum Hormones ◽  
Idiopathic Infertility ◽  
Infertile Men ◽  
Comorbidity Index

Abstract Study question We aimed to investigate the rate of and the clinical characteristics of men with idiopathic versus unexplained infertility from a cohort of white-European men. Summary answer Approximately 20% and 5% of men evaluated for primary couple’s infertility depicted characteristics suggestive for idiopathic and unexplained infertility, respectively. What is known already Male factor infertility (MFI) can be associated with clinical, hormonal and genetic diseases, but MFI is idiopathic in almost 30% of cases. Study design, size, duration Data from 3098 infertile men (according to WHO definition) consecutively evaluated between 2003-2020 at a single academic centre were analysed and compared with those of 103 fertile controls. Health-significant comorbidities were scored with the Charlson Comorbidity Index (CCI). Idiopathic infertility was defined for pathological semen analysis but normal physical examination and endocrine, genetic and biochemical laboratory testing. Unexplained infertility is defined as infertility of unknown origin with normal sperm parameters. Participants/materials, setting, methods Testicular volume (TV) was assessed with a Prader’s orchidometer. Serum hormones and sperm DNA fragmentation index (SDF) were measured in every patient. Vitamin D3 (VitD) deficiency was considered for vitD levels <20 ng/mL. Semen analyses were based on the 2010 WHO reference criteria. Health-significant comorbidities were scored with the Charlson Comorbidity Index (CCI). Main results and the role of chance Overall, 570 (18.5%) and 154 (5.0%) patients depicted criteria suggestive for either idiopathic or unexplained primary infertility, respectively. Groups were similar in terms of age, BMI, CCI, recreational habits, circulating serum hormones and SDF. Testicular volume was lower in men with idiopathic vs. unexplained infertility [median (IQR) 20 (15-25) vs. 20 (17-25); p < 0.001]; more idiopathic than unexplained infertile men depicted TV < 15ml (23.4% vs. 12%; p < 0.01). Similarly, vitD levels were lower [22 (17-28) vs. 27 (21-42) ng/mL; p < 0.001] in idiopathic vs. unexplained infertile men, with a higher rate of pathologic VitD levels in the same group (42.1% vs. 10%; p = 0.04). When compared to fertile controls, groups were similar in terms of age, BMI, CCI and serum hormones values. TV was larger in fertile controls than idiopathic and unexplained infertile men (all p < 0.01). At multivariable logistic regression analysis only vitD deficiency (OR 8.1, p = 0.03) was found to be associated with idiopathic infertility after accounting for age, BMI, testosterone values and TV.  Limitations, reasons for caution The small number of fertile controls may raise the possibility of biases. Wider implications of the findings Idiopathic and unexplained infertility were identified in approximately 20% and 5% of men evaluated, respectively. Idiopathic infertile men showed lower TV and lower vitD values compared to men with unexplained infertility. Future studies are needed to develop a more tailored management to these difficult MFI cases. Trial registration number .

scholarly journals Semen quality pattern and age threshold: a retrospective cross-sectional study of 71,623 infertile men in China, between 2011 and 2017

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
W. N. Li ◽  
M. M. Jia ◽  
Y. Q. Peng ◽  
R. Ding ◽  
L. Q. Fan ◽  
...  
Keyword(s):  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Cross Sectional Study ◽  
Semen Parameters ◽  
Sectional Study ◽  
Joinpoint Regression ◽  
Cross Sectional ◽  
Progressive Motility ◽  
Infertile Men

Abstract Background The aim of this study was to provide information on the semen quality pattern of infertile men and age thresholds for semen parameters in China. Methods This was a retrospective cross-sectional study investigating 71,623 infertile men from the Reproductive and Genetic Hospital of CITIC Xiangya in Hunan, China, from 2011 to 2017. The Kruskal-Wallis test, Mann-Kendall test, linear regression model and joinpoint regression were used. Results Although erratic changes were observed in the median semen parameters (sperm concentration 40.1–52.1 × 106/ml, total sperm count 117.8–153.1 × 106, sperm progressive motility 33.4–38.1%) during the 7 years of observation, no significant decrease in semen quality was found, and 47.88% of infertile men showed normal semen parameters according to the World Health Organization (WHO) criteria. According to the joinpoint regression analysis, sperm progressive motility appeared to decrease earlier than the sperm concentration and total sperm count (at 28, 58, and 42 years of age, respectively). Conclusions There is no evidence of a deterioration in semen quality among infertile men in Hunan, China. Semen parameters decreased with increasing age, with turning points noted at different ages. Semen parameters are not absolute evidence for the assessment of male fertility potential. Therefore, we believe that, among semen parameters, the sperm concentration is the best predictor of fertility for ART, followed by motility. Decreased sperm motility may affect natural pregnancy, but it is not necessary for successful IVF.

scholarly journals Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes

Biomédica ◽  
2021 ◽  
Vol 41 (4) ◽  
Author(s):  
Omid Jazayeri ◽  
Setareh Farahmand Araghi ◽  
Tahereh A. Aghajanzadeh ◽  
Fereshteh Mir Moammadrezaei
Keyword(s):  
Gene Expression ◽  
Aqueous Extract ◽  
Sperm Count ◽  
Mrna Level ◽  
Treated Group ◽  
Sperm Number ◽  
Infertile Men ◽  
Anti Cancer ◽  
Insight Into ◽  
Mouse Body Weight

Introduction: Broccoli (Brassica oleracea) is well recognized due to its properties as an anti-cancer, antioxidant and scavenging free radicals. However, its benefit in enhancing spermatogenesis is not well understood. Objectives: To investigate the effect of broccoli aqueous extract on sperm factors and also expression of the involving genes (Catsper1, Catsper2, Arl4a, Sox5 and Sox9) in sperm factors in mice. Material and methods: Male mice were divided randomly into six groups: (1) Control, (2) Cadmium (3 mg/kg mouse body weight), (3) Orally treated with 200 broccoli aqueous extract (1 g ml-1), (4) Orally treated with 400 µl of broccoli aqueous extract, (5) Orally treated with 200 broccoli aqueous extract plus cadmium, and (6) Orally treated with 400 µl of broccoli aqueous extract plus cadmium. Sperms factors and also gene expression in Catsper1, Catsper2, Arl4a, Sox5 and Sox9 genes were studied. Results: An obvious improvement in sperm number and slight enhancement in sperm motility was observed in mice treated with broccoli extract with and without cadmium. While sperm viability was reduced by broccoli extract, except for 200 µl of broccoli extract with cadmium that was significantly increased. Interestingly, Arl4a gene expression showed an increase in 400 µl broccoli-treated group. Likewise, the Arl4a mRNA level in mice treated with cadmium along with 200 µl broccoli extract was higher than in cadmium-treated mice. Furthermore, broccoli extract enhanced the mRNA level of Catsper2 and Sox5 genes in mice treated with both 200 and 400 µl broccoli extract along with cadmium than the only cadmium-treated group. Conclusion: Generally, improvement in sperm count in broccoli-treated mice provides insight into the pharmaceutical industry to make new products available to infertile men.

scholarly journals The possible association of clusterin fucosylation changes with male fertility disorders

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ewa Janiszewska ◽  
Izabela Kokot ◽  
Iwona Gilowska ◽  
Ricardo Faundez ◽  
Ewa Maria Kratz
Keyword(s):  
Male Infertility ◽  
Blood Serum ◽  
Seminal Plasma ◽  
Male Fertility ◽  
Sperm Count ◽  
Semen Analysis ◽  
Infertile Men ◽  
Abnormal Sperm ◽  
Fertility Disorders ◽  
Oligosaccharide Structures

AbstractIn the seminal plasma (n = 118) and serum (n = 90) clusterin (CLU) the fucosylation and the expression of selected fucosyltransferases (FUTs) were analyzed. Samples from infertile men were divided into groups based on the results of the standard semen analysis: normozoospermic (N), teratozoospermic (T), asthenoteratozoospermic (AT) and oligoasthenoteratozoospermic (OAT). The CLU fucosylation was analyzed using lectin-ELISAs with biotinylated lectins specific to α1,3-, α1,2-linked antennary fucose, and α1,6-linked core fucose (LTA, UEA, and LCA, respectively). The concentrations of FUT3 and FUT4, reflecting the expression of Le oligosaccharide structures, were measured using ELISA tests. The differences in serum CLU and FUT4 concentrations, and in the expression of core fucose and antennary fucose α1,2-linked in CLU glycans between the N group and other groups examined suggest that the disturbances in sperm count, motility, and morphology are not the only cause of male infertility. Lack of similarities between levels of examined parameters in blood serum and seminal plasma may suggest the differences in mechanisms leading to glycoproteins glycosylation. It confirmed the observed differences in concentrations of seminal plasma CLU, FUT3, and FUT4 between the OAT group and N, T, AT groups, indicating that decreased sperm count may be related to these parameters expression. The serum CLU concentrations and expression of core fucose and fucose α1,2-linked in CLU, seem to be good markers differentiating normozoospermic men from those with abnormal sperm parameters, which was not observed for seminal plasma.

scholarly journals Role of Dietary Antioxidants and Vitamins Intake in Semen Quality Parameters: A Cross-sectional Study

2020 ◽  
Author(s):  
Reza Ghiasvand ◽  
Laleh Dehghan Marvast ◽  
Mehran Nouri ◽  
Aida Jahanbakhsh ◽  
farahnaz haeri
Keyword(s):  
Dietary Intake ◽  
Semen Quality ◽  
Sperm Count ◽  
Cross Sectional Study ◽  
Quality Parameters ◽  
Dietary Antioxidants ◽  
Cross Sectional ◽  
Infertile Men ◽  
Semen Volume

Abstract Objective Infertility had an increasing trend between couples in Iran. Several factors such as lifestyle, physical activity, unhealthy dietary habits and stress are associated with production of ROS in seminal fluid. The aim of this study was to identify the role of dietary antioxidants and vitamins intake on semen quality parameters, among Iranian infertile men. Methods This cross-sectional study was performed on 400 newly diagnosed infertile men in Yazd Reproductive sciences Institute from July 2019 to December 2019. The presence of infertility was confirmed by an expert andrologist, based on WHO criteria. Dietary antioxidants and vitamins intake were assessed using a 168 items semi-quantitative food frequency questionnaire (FFQ). Results We found an association between semen volume and Alpha Carotene dietary intake, total motility and Beta Cryptoxanthin dietary intake, and sperm count with riboflavin intake (P = 0.03). Conclusions We concluded that dietary intake of Alpha Carotene, Beta Cryptoxanthin, and Riboflavin are related to sperm count, semen volume and total motility in Iranian infertile men, respectively. Our data suggest that that adhering to a diet that is rich in sources of antioxidant and vitamin can have a positive effect on men’s sexual health. However, more research is needed to confirm these relations and provide the evidence needed to exert these findings into clinical practice.

scholarly journals Y chromosome microdeletions in infertile male candidates for microfertilization

2008 ◽  
Vol 136 (3-4) ◽  
pp. 126-130
Author(s):  
Momcilo Ristanovic ◽  
Vera Bunjevacki ◽  
Cane Tulic ◽  
Ivana Novakovic ◽  
Tatjana Ille ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Sperm Count ◽  
Gene Mutations ◽  
Control Group ◽  
Specific Primers ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Infertile Men ◽  
Y Chromosome Microdeletions ◽  
Polymerase Chain

Introduction Y chromosome microdeletions are the second most frequent genetic cause of male infertility after Klinefelter's syndrome. Objective The aim of the study was to determine the frequency of Y chromosome microdeletions in a group of infertile men with an idiophatic cause of infertility, candidates for microfertilization (Intra-cytoplasmic Sperm Injection - ICSI) in Serbia and to correlate genotype-phenotype in patients with Y chromosome microdeletions. METHOD One hundred and sixty patients with low sperm count (less than 5x106 spermatozoa/ml) were enrolled in the study. Forty patients were excluded from the study: ten because they were diagnosed with cytogenetic abnormality and thirty patients were diagnosed with other known causes of infertility. The control group consisted of 150 men who fathered at least one child in the last two years. Genomic DNA was extracted from peripheral blood samples and two multiplex polymerase chain reactions (PCR) analyses were performed using specific primers to confirm the presence or absence of Y chromosome microdeletions. Results Microdeletions were detected in 12 of 120 (10%) cases, while no deletions were detected in the control group. Of total number of 12 deletions, nine were detected in AZFc region (75%), one in AZFa (8%), and two in AZFbc (17%). Conclusion Testing for Y chromosome microdeletions should be considered as an important element in diagnosis and genetic counselling of infertile couples in Serbia. Decisions regarding the assisted reproduction should be made based on the detailed clinical, endocrinological and cytogenetic examinations, spermogram, presence or absence and type of AZF microdeletions and CFTR gene mutations. .

scholarly journals Assessment of Steroid 5 Alpha Reductase Enzyme Levels and Its Correlation with Sex Hormones in Infertile Iraqi Men

2020 ◽  
Vol 7 (6) ◽  
pp. 227-233
Author(s):  
Fakhry Gazal Mohammad ◽  
Adnan F. AL-Azzawie
Keyword(s):  
Sex Hormones ◽  
Seminal Fluid ◽  
Sperm Count ◽  
Age Groups ◽  
Significant Negative Correlation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Infertile Men ◽  
Normal Spermatogenesis ◽  
Low Levels

This study aims to evaluate the 5-alpha reductase enzyme (SRD5A2) levels in serum and it’s association with some sex hormones such as Follicle stimulating hormone (FSH), Luteinizing hormone (LH), prolactin and testosterone hormone and the infertility type in some infertile Iraqi men. Blood and seminal fluid samples from (60) idiopathic male infertile and (30) healthful individuals as a control group aged (18 to 60 year) are collected from private clinics. Enzyme Linked Immunosorbent Assay (ELISA) has been used for estimation of serum SRD5A as enzymes and FSH, LH, prolactin and testosterone as hormones.According to types of sperm count patients have been divided to two group 49 person as azoospermia group (zero /ml) and 11 person as oligospermia group less than 20 million/ml. The levels of SRD5A2 and testosterone are significantly decreased to (P≤0.01) in the infertile men as compared with control group. While the level of FSH, LH and prolactin are significantly higher (P≤ 0.01) in the infertile men than control group. The results of SRD5A2 are non-significant, while, levels of FSH, LH and prolactin are higher significant (P≤ 0.01) except testosterone level which has significant differences (P≤ 0.05) in the age groups <20-30 year, 31–40 year and ≥ 40 year. There are significant differences (P≤ 0.01) in levels of SRD5A2, FSH and prolactin, but the levels of LH are non-significant in the infertility period ≤ 10 year, 11 – 20 year, ≥ 20 year. Smoker infertile men have low levels (P≤ 0.01) in the SRD5A2 and prolactin while have high levels in the LH hormones compared with control. Patients with family history have shown significant differences (P≤0.05) in the levels of SRD5A2, testosterone, LH and prolactin. In conclusion, this study revealed significantly decrease in the levels ofSRD5A2 in the Azoospermic and oligospermic infertile men and significant negative correlation (P<0.05) between SRD5A2 pg/ml and FSH (mIu/ml) R factor-0.328. Therefore, SRD5A2 has important role in the diagnosis of idiopathic male infertility and it's one of the important markers in the diagnosis of normal spermatogenesis.

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