scholarly journals Ameliorative Effect of L-Arginine as Antioxidant Agent on Hematological and Testicular Functions in Lead-Induced Toxicity in Male Rats

2018 ◽  
pp. 1-8
Author(s):  
Marwan A. Ibrahuim
Keyword(s):  
Binding Capacity ◽  
Iron Status ◽  
Hematological Parameters ◽  
Protective Role ◽  
Male Rats ◽  
Daily Injection ◽  
Heme Synthesis ◽  
Ameliorative Effect ◽  
Iron Binding Capacity ◽  
Induced Changes

Aims: The present study aimed to elucidate the protective role of L-Arginine on lead – induced suppression of the pathway of heme synthesis and the toxicity of lead on testis. Methodology: The current study included two experiments; the first was carried out on two groups to follow up the changes that could occur in iron status and reproductive system of male rats because of the lead exposure. The second experiment, three comparisons were made between three groups of rats with lead treatment only and other three groups rats with lead treatment followed by additional of daily injection of L-Arginine intraperitoneal about 500 mg L-Arginine / kg.b.wt. / day for 10, 20 and 30 days after the end of lead treatment. Results: Lead significantly abolished heme synthesis presented by decrease in Hb, Fe and ceruloplasmin (Cp) and elevation in total iron binding capacity (TIBC) and serum ferritin. Furthermore, testosterone (total and free) significantly decreased after rats treated with 1% lead acetate while, estradiol (E2) and acid phosphatase (total and prostatic) were elevated. The concentration of dehydroepiandrosterone sulphat (DHEA-S) did not change after lead treatment. Conclusion: Daily treatment with 500 mg L-Arginine/kg body weight prevented the suppressive effects of lead on hematological parameters and testicular functions. The mechanism of L-Arginine action on lead induced changes was attributed to protection of antioxidant capacity in cells in addition to the ability of L-Arginine to scavenge free radicals and acts as cell membrane stabilizer.

scholarly journals Low Serum Iron Status Is Associated With Low Incidence of Coronary Artery Disease In Women

2022 ◽  
Author(s):  
Shuren Guo ◽  
Xiaohuan Mao ◽  
Xiaohua Li ◽  
Huan Ouyang
Keyword(s):  
Coronary Artery ◽  
Serum Level ◽  
Iron Metabolism ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Protective Role ◽  
Artery Disease ◽  
Iron Binding Capacity ◽  
Low Serum

Abstract Background: Disorders of iron metabolism has been implicated in cardiovascular disease. However, the association of serum ferritin and coronary artery disease (CAD) remains inconsistent. Here, we investigated the associations of serum iron metabolism with the incidence of CAD, the severity of coronary artery stenosis, metabolic biomarkers, and 1-year restenosis after coronary artery revascularization. Methods: A total of 643 CAD patients and 643 healthy controls were enrolled to assess the associations of serum iron status with the presence of CAD, the severity of CAD, and 1-year rehospitalization after revasculation. Serum iron metabolism and other metabolic markers were measured in all subjects. All statistical analyses were analyzed using SPSS22.0 software and STATA statistical package.Results: Serum level of iron metabolism markers, including serum iron, ferritin, unsaturated transferrin iron binding capacity (UIBC), Total iron binding capacity (TIBC) levels, in CAD groups was significantly higher than the control group (P<0.001). UIBC and TIBC were negatively correlated with ferritin in both sexes. Serum level of iron (OR=0.806, 95% CI (0.687-0.944), UIBC (OR=0.919, 95% CI (0.852-0.992), and TIBC (OR=0.864, 95% CI (0.787-0.95) were found to have a protective role for CAD in women (P<0.05, Table 3). The OR for ferritin was significant in the both sexes (OR=1.029, 95% CI (1.002-1.058) in men, OR=1.02, 95% CI (1.005-1.034) in women, P<0.05). Conclusion: Low Serum level of iron, UIBC, TIBC and ferritin levels were found to have a protective role for CAD in women, but not in men. Elevated serum ferritin is independently and positively associated with CAD in men and women.

The protective role of luteolin against the methotrexate-induced hepato-renal toxicity via its antioxidative, anti-inflammatory, and anti-apoptotic effects in rats

2021 ◽  
pp. 096032712199190
Author(s):  
AA Dar ◽  
A Fehaid ◽  
S Alkhatani ◽  
S Alarifi ◽  
WS Alqahtani ◽  
...  
Keyword(s):  
Renal Toxicity ◽  
Histopathological Examination ◽  
Protective Role ◽  
Male Rats ◽  
Antioxidant Enzyme Activities ◽  
Serum Samples ◽  
Liver And Kidney ◽  
Anti Inflammatory ◽  
Induced Changes ◽  
Apoptotic Effects

Methotrexate (MTX) is frequently used drug in treatment of cancer and autoimmune diseases. Unfortunately, MTX has many side effects including the hepato-renal toxicity. In this study, we hypothesized that Luteolin (Lut) exhibits protective effect against the MTX-induced hepato-renal toxicity. In order to investigate our hypothesis, the experiment was designed to examine the effect of exposure of male rats to MTX (20 mg/kg, i.p., at day 9) alone or together with Lut (50 mg/kg, oral for 14 days) compared to the control rats (received saline). The findings demonstrated that MTX treatment induced significant increases in the liver and kidney functions markers in serum samples including Aspartate transaminase (AST), Alanine transaminase (ALT), creatinine, urea and uric acid. MTX also mediated an oxidative stress expressed by elevated malondialdehyde (MDA) level and decreased level of reduced glutathione (GSH), antioxidant enzyme activities, and downregulation of the Nrf2 gene expression as an antioxidant trigger. Moreover, the inflammatory markers (NF-κB, TNF-α, and IL-1β) were significantly elevated upon MTX treatment. In addition, MTX showed an apoptotic response mediated by elevating the pro-apoptotic (Bax) and lowering the anti-apoptotic (Bcl-2) proteins. All of these changes were confirmed by the observed alterations in the histopathological examination of the hepatic and renal tissues. Lut exposure significantly reversed all the MTX-induced changes in the measured parameters suggesting its potential protective role against the MTX-induced toxicity. Finally, our findings concluded the antioxidative, anti-inflammatory and anti-apoptotic effects of Lut as a mechanism of its protective role against the MTX-induced hepato-renal toxicity in rats.

Iron status of adolescent girls from two boarding schools in southern Benin

2008 ◽  
Vol 11 (7) ◽  
pp. 737-746 ◽  
Author(s):  
Halimatou Alaofè ◽  
John Zee ◽  
Romain Dossa ◽  
Huguette Turgeon O’Brien
Keyword(s):  
Iron Deficiency ◽  
Vitamin C ◽  
Adolescent Girls ◽  
Binding Capacity ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Meat Consumption ◽  
Boarding Schools ◽  
Important Health ◽  
Iron Binding Capacity

AbstractIron deficiency (ID) is the most prevalent micronutrient deficiency in the world, particularly in developing countries. Blood samples and a qualitative FFQ on Fe- and vitamin C-rich foods were obtained in 180 adolescent girls aged 12 to 17 years living in two boarding schools from south Benin. ID, defined as serum ferritin either <20μg/l or 20–50μg/l, plus two of the following parameters: serum Fe<11μmol/l, total iron-binding capacity>73μmol/l or transferrin saturation<20%, was found in 32% of subjects. Anaemia (Hb<120g/l) was found in 51% of adolescents, while 24% suffered from iron-deficiency anaemia (IDA) (ID and Hb<20g/l). After adjusting for confounding factors (age, mother's and father's occupation, household size) in a logistic regression equation, subjects having a low meat consumption (beef, mutton, pork) (<4 times/week) were more than twice as likely to suffer from ID (OR=2·43; 95% CI 1·72, 3·35;P=0·04). Adolescents consuming less fruits (<4 times/week) also had a higher likelihood of suffering from ID (OR=1·53; 95% CI 1·31, 2·80;P=0·03). Finally, subjects whose meat consumption was low were twice as likely to suffer from IDA (OR=2·24; 95% CI 1·01, 4·96;P=0·04). The prevalence of ID represents an important health problem in these Beninese adolescent girls. A higher consumption of Fe-rich foods and of promoters of Fe absorption (meat factor and vitamin C) is recommended to prevent ID deficiency in these subjects.

scholarly journals Iron Metabolism Modification During Repeated Show Jumping Event in Equine Athletes

2017 ◽  
Vol 17 (1) ◽  
pp. 197-204 ◽  
Author(s):  
Giuseppe Piccione ◽  
Maria Rizzo ◽  
Francesca Arfuso ◽  
Daniele Bruschetta ◽  
Elisabetta Giudice ◽  
...  
Keyword(s):  
Health Status ◽  
Physical Performance ◽  
Binding Capacity ◽  
Iron Status ◽  
Recovery Period ◽  
Hemoglobin Concentration ◽  
Repeated Measure ◽  
Iron Binding ◽  
Iron Binding Capacity ◽  
Show Jumping

Abstract In athletic horse the evaluation of iron status is of great importance to improve physical performance and health status of animal. The aim of this study was to evaluate the changes of iron indices following show jumping. Ten regularly trained Italian Saddlebred horses aged 7-8 years (mean body weight 467±12 kg) were subjected to three days jumping competition. Blood samples were collected at 5 time points: T0 (the day before competitions), T1 (immediately after exercise at day 1), T2 (immediately after exercise at day 2), T3 (immediately after exercise at day 3) and during the recovery period T4 (24 h after day 3). On each blood sample the values of red blood cell (RBC), hemoglobin concentration (Hb), hematocrit (Hct), serum iron, ferritin, transferrin, total iron-binding capacity (TIBC) and unsaturated iron-binding capacity (UIBC) were assessed. One-way repeated measure analysis of variance (ANOVA) showed a statistical significant effect of exercise (P<0.05) on all studied parameters. The application of Bonferroni’s post-hoc comparison showed a statistical significant increase in all studied parameters after exercise. These results provide new information about the changes in iron profile of jumper horse following exercise allowing for better evaluation of the health status and physical performance of this athlete horse.

scholarly journals Effects of Lead on Hemopoeitic and Iron Status in Iraqi Workers at Lead Batteries Factory

2013 ◽  
Vol 10 (2) ◽  
pp. 388-395
Author(s):  
Baghdad Science Journal
Keyword(s):  
Aminolevulinic Acid ◽  
Early Stage ◽  
Binding Capacity ◽  
Iron Status ◽  
Hemoglobin Concentration ◽  
Blood Lead ◽  
Significant Negative Correlation ◽  
Control Group ◽  
Lead Levels ◽  
Iron Binding Capacity

The present study aimed to evaluate the effect of lead exposure on hemopoetic system (through the index delta-Aminolevulinic acid dehydratase ?-ALAD activity & hemoglobin concentration (Hb) ) and on iron status (levels of iron Fe, Ferritin Fr, Total iron binding capacity TIBC, percentage of transferine saturation TF%) in 44 Iraqi worker at lead batteries factory. Workers divided into two groups: smokers(n=21) mean aged (37.33±4.82 year)and non smokers(n=23) mean aged(40.78±7.89 year) and 45 healthy subjects mean aged (33.97±5.08)as control group . Activity of ?-ALAD ratio shows significant decrease (p ? 0.05) ,while Hb and hematocrit Hct were non significant (p ? 0.05) in smoker workers more than non smoker as compared to control . The results show a non significant decrease (p ? 0.05) in the sera levels of iron S.Fe, TS%, Fr in all workers as compared to control , the amount of decreasing for smoker more than non smoker except ferritin levels. A significant increases in the level of TIBC in workers as compared to control, this elevation is more in smoker than non smoker workers. In smoker workers, there was a significant positive correlation between the blood lead levels BLLs and Hb, Hct,TIBC whereas a significant negative correlation was observed between BLLs and S.Fe, TS%. In conclusion, the results of this study reveal the importance of monitoring the level of iron status in smoker peoples who dealing or exposure to lead due to the probability injured with anemia and used ?-ALAD ratio to predict the efficiency of their heme synthesis as a new marker for the diagnosis of early stage of anemia.

scholarly journals NEOPTERIN AND INTERFERON-GAMMA AS IMMUNE RESPONSE MARKERS IN BETATHALASSEMIA MAJOR PATIENTS

2018 ◽  
Vol 11 (9) ◽  
pp. 192
Author(s):  
Shahad F Obeid ◽  
Sanad B Al-a'araji ◽  
Bassam F Matti ◽  
Hayder Adnan Fawzi
Keyword(s):  
Immune System ◽  
Interferon Gamma ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
System Response ◽  
Iron Binding ◽  
Ifn Γ ◽  
Immune System Response ◽  
Iron Binding Capacity

Objective: The assessment of neopterin and interferon-gamma (IFN-γ) levels as a part of immune system response about serum iron status in β-thalassemia (TM) major patients.Methods: Spectrophotometry applied for the estimation of iron status including serum iron level, total iron-binding capacity, and unsaturated iron-binding capacity. Enzyme-linked immunosorbent assay (ELISA) applied for the estimation of the serum cytokines included neopterin and IFN-γ also serum ferritin estimation by ELISA from 130 β-TM major patients where they divided according to serum ferritin level (< and ≥2500 ng/mL).Results: The neopterin and IFN-γ concentration showed significantly increased with direct correlation among TM patient group when compared to the normal healthy control group. However, there was no significant difference between different levels of serum ferritin.Conclusion: The increased serum level of neopterin and IFN-γ found in patients with β-TM may be due to the direct effect of iron on cellular immune cells beside of immune system response to the effect of iron toxicity on different body organs. There was a direct moderate relationship between neopterin and IFN-γ.

scholarly journals Evaluation of Erythrocytes, Platelets, and Serum Iron Profile in Dogs with Chronic Enteropathy

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Veronica Marchetti ◽  
George Lubas ◽  
Andrea Lombardo ◽  
Michele Corazza ◽  
Grazia Guidi ◽  
...  
Keyword(s):  
Serum Iron ◽  
Activity Index ◽  
Binding Capacity ◽  
Iron Status ◽  
Disease Activity Index ◽  
Microcytic Anemia ◽  
Chronic Enteropathy ◽  
Before And After ◽  
Iron Binding Capacity ◽  
Iron Profile

The aim of this study is to evaluate iron status, erythrocyte, and platelet modifications in dogs with chronic enteropathy (CE). Dogs were grouped as food-responsive diarrhea (FRD, ), antibiotic-responsive diarrhea (ARD, ), and steroid-responsive diarrhea (SRD, ) relating to therapeutic-response. Clinical and haematological findings, evidence of gastrointestinal blood loss, and iron metabolism were evaluated before and after treatment. A mild normocytic or microcytic anemia and thrombocytosis were identified, respectively in 18.0% and 31.8% of CE dogs. No significant differences between pre- and posttreatment of hematocrit, haemoglobin, and mean corpuscular volume, platelet count and mean platelet volume were found. Statistical analysis pointed out significant differences between pre- and posttreatment in serum iron () and unsaturated iron binding capacity (UIBC) (). No significant correlations were found between these parameters and canine Inflammatory Bowel Disease activity index and pattern of CE as well.

scholarly journals Magnesium supplementation and iron status among female students: the intervention study

2021 ◽  
Author(s):  
Neda Milinković
Keyword(s):  
Polynomial Regression ◽  
Complete Blood Count ◽  
Binding Capacity ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Principal Component ◽  
Female Students ◽  
Iron Binding ◽  
Healthy Female ◽  
Iron Binding Capacity

Background: Literature data indicate the benefit of magnesium (Mg) supplementation. The aim of this study was to examine the effect of short-term Mg supplementation on iron status in healthy female participants. Methods: One hundred healthy female students of the University of Belgrade - Faculty of Pharmacy participated in the study during eleven intervention days. Students ingested Mg preparations with the same dose of the active substance. The analysis included the measurement of serum iron, unsaturated iron binding capacity (UIBC), total iron binding capacity (TIBC), total Mg (tMg), ionized Mg (iMg), complete blood count, met-, carboxy- and oxy-haemoglobin (metHgb, COHgb, O2Hgb). Transferrin concentrations and the percentage of transferrin saturation (SAT) were calculated manually. The association among the analyzed biochemical parameters was examined using polynomial regression. A principal component analysis (PCA) was used for the evaluation of interdependence between the analyzed parameters. Results: A statistically significant trend for change in O2Hgb (%) by tertiles of iMg concentrations was found (P = 0.029). Serum tMg reached a significant positive correlation with the SAT at concentration levels greater than 0.9 mmol/L, after 11 days of intervention (R2=0.116). Ionized Mg in a concentration higher than 0.6 mmol/L is positively correlated with SAT and serum Fe (R2=0.214; 0.199, respectively). PCA revealed variability of 64.7% for two axes after 11 days. Conclusions: Mg supplementation leads to an improvement in certain iron status parameters even in individuals with optimal levels of these indices. However, caution should be exercised when supplementing Mg, and laboratory monitoring of the interaction is required.

Aniline: early indicators of toxicity in male rats and their relevance to spleen carcinogenicity

2004 ◽  
Vol 23 (8) ◽  
pp. 379-389 ◽  
Author(s):  
W Mellert ◽  
K Deckardt ◽  
C Gembardt ◽  
I Zwirner-Baier ◽  
R Jäckh ◽  
...  
Keyword(s):  
Haemolytic Anaemia ◽  
Iron Metabolism ◽  
Binding Capacity ◽  
Male Rats ◽  
Cell Counts ◽  
Histopathologic Evaluation ◽  
Early Effects ◽  
Iron Binding Capacity ◽  
Dose Levels ◽  
Early Indicators

Early indicators of aniline hydrochloride (AH) toxicity were investigated in male Fisher 344 rats for 1 or 4 weeks at dietary dose levels of 10, 30 or 100 mg/kg body weight (bw)/day (actual intake at least 6, 17 and 57 mg/kg). The doses were based on earlier studies that had shown spleen toxicity and carcinogenicity in male rats at 100 mg/kg/day but not at 10 mg/12/kg/day. In the present study a dose-related formation of haemoglobin adducts and Heinz bodies was found from 10 and 30 mg/kg bw/day, respectively, onwards. Signs of anaemia (decreased red blood cell counts and increased reticulocytes) were recorded from 30 mg/kg onwards. At 100 mg/kg, an overt haemolytic anaemia was associated with increases in serum transferrin concentration and total iron binding capacity in the blood reflecting major perturbations in iron metabolism. At this dose there was an increase in peripheral neutrophil leucocytosis in the blood, indicating an inflammatory process in the spleen. Histopathologic evaluation showed a focal perisplenitis and haemosiderin deposition in sinusoidal Kupffer cells of the liver at 100 mg/kg. These results corroborate the contention that carcinogenic doses of aniline cause early effects on haematological parameters, inflammatory reaction in the spleen and perturbations in iron metabolism as a result of haemolytic anaemia. Accordingly, the carcinogenicity of aniline may be linked to definable threshold-related processes.

Sign in / Sign up

Export Citation Format

Share Document