Effect of Aqueous Extract of Costus afer Stems on the Liver and Cardiac Enzymes Activities of High Fat Diet Induced Hyperlipidemic Rats

Aim: The present study was carried out on the effect of aqueous extract of Costus afer stems on the liver and cardiac enzymes activities of diet induced hyperlipidemic rats. Methodology: Wistar albino male rats (100-135g) were randomly distributed into 7 groups of 12 rats each. Group I was fed with standard diet as normal control rats and all the other groups were fed with high fat diet (10g eggyolk/day) for 2 weeks. The plant extract was administered orally at different concentrations of 400, 800 and 1600mg/kg b.w alone and also in combination with the reference drug, Atorvastatin® (0.26mg) to the treatment groups for four weeks. The liver and cardiac enzymes activities were observed at specific intervals (2 weeks). Results: Results showed a significant (p<0.05) difference in the plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities after two weeks of feeding with egg yolk supplemented diet in all the test groups when compared with the normal and hyperlipidemic control rats. After 2 and 4weeks treatment, there was a significant (p<0.05) decrease in the plasma AST, ALT and ALP activities of rats in groups IV, V and VII when compared with the normal and hyperlipidemic control rats (groups I and II). There was also a significant (p<0.05) difference in the creatine phosphokinase (CPK) activity of all the test groups when compared with the normal control rats after feeding with high fat diet for 2 weeks. After 2 and 4weeks of treatment, rats in groups III, IV and VI (HTR on atorvastatin, 0.26mg, HTR on aqueous extract, 400 mg/kg and 1600 mg) significantly decreased when compared with the normal and hyperlipidemic control rats. Conclusions: Hence, this shows that Costus afer stem extract may prevent hepatocyte injury or also maybecardioprotective against myocardial injury.

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Effect of Aqueous Extract of Costus afer Stems on the Serum Proteins and Bilirubin Levels of High Fat Diet Induced Hyperlipidemic Rats

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2020/v6i230150 ◽

2020 ◽

pp. 57-64

Author(s):

Anyiam C. Chioma ◽

Anyiam C. Albert ◽

Onyeneho R. Ijeoma

Keyword(s):

Normal Control ◽

Aqueous Extract ◽

Plant Extract ◽

Total Protein ◽

High Fat Diet ◽

Serum Proteins ◽

High Fat ◽

Group I ◽

Significant Difference ◽

Costus Afer

Aim: The effect of aqueous extract of Costus afer stems on total protein, albumin,globulin, total and conjugated bilirubin levels in diet induced hyperlipidemic rats were studied. Methodology: Wistar albino male rats (100-135 g) were randomly distributed into 7 groups of 12 rats each. Group I was fed with standard diet as normal control rats and all the other groups were fed with high fat diet (10 g eggyolk/day) for 2 weeks. The plant extract was administered orally at different concentrations of 400, 800 and 1600mg/kg b.w alone and also in combination with the reference drug, Atorvastatin® (0.26mg) to the treatment groups for four weeks. The serum proteins and bilirubin were observed at specific intervals (2 weeks). Results: The results revealed significant (p<0.05) increase in the total protein, albumin and globulin concentration after 2 weeks of feeding with high fat diet in rats which were in groups IV and V as compared to normal control rats. Treatment of rats with plant extract showed no significant difference in the total protein concentration of hyperlipidemic test rats while the albumin concentration of rats in group VII increased significantly when compared to the normal and hyperlipidemic control rats. There was no significant difference in the globulin concentration of hyperlipidemic treated rats. After 2nd and 4th week of treatment, the total bilirubin concentrations of rats in groups V and VI (HTR on aqueous extract, 1600 mg/kg and HTR on aqueous extract, 800 mg/kg) decreased significantly when compared with normal and hyperlipidemic control rats. After 2 weeks of treatment, the conjugate bilirubin concentration of rats in group VI (HTR on aqueous extract, 1600 mg/kg) significantly (p<0.05) decreased when compared to the hyperlipidemic control rats. Conclusions: Hence, this shows that Costus afer stem extract does not have any deleterious effect on tissues and on the analyzed parameters.

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Cardioprotective Effect of Ajwa Date Aqueous Extract on Doxorubicin-Induced Toxicity in Rats

Biomedical & Pharmacology Journal ◽

10.13005/bpj/1519 ◽

2018 ◽

Vol 11 (3) ◽

pp. 1521-1536 ◽

Cited By ~ 1

Author(s):

Meaad F. Sabbah ◽

Fawzia Alshubali ◽

Othman A. S. Baothman ◽

Mazin A. Zamzami ◽

Lobna Shash ◽

...

Keyword(s):

Lipid Profile ◽

Aqueous Extract ◽

Chemotherapeutic Agents ◽

Male Rats ◽

Control Group ◽

Dna Integrity ◽

Protective Effects ◽

Cardiac Enzymes ◽

Group Iii ◽

Group I

Doxorubicin (DOX) is one of the most potent and widely used chemotherapeutic agents to treat several malignancies. However, the clinical use of DOX is seriously restricted due to its acute and chronic cardiotoxic side effects This study investigated the protective effect of (Ajwa) date aqueous extract (AJDAE) against doxorubicin-induced cardiotoxicity in rats. Sixty Wister albino male rats (150-200 gms.) were comprised in our study and divided into six equal groups: group I (untreated control), group II, group III, rats were orally received AJDAE (0.75 & 1.5 gm/ kg.bw) respectively, for 4 weeks, rats of groups IV, V and VI were intraperitoneally injected with one dose of doxorubicin (5 mg/kg.bw) at the end of the 4th week of the study to induce cardiotoxicity, rats of groups V & VI were orally received AJDAE (0.75 & 1.5 gm/ kg.bw) respectively. Cardiac enzymes, lipid profile, SOD, GR, GST, GPx, CAT and MDA in rats’ hearts homogenate, urinary 8OHdG as well as DNA integrity and histopathological changes were investigated in all studied rats.Oral administration of AJDAE (0.75 & 1.5 gm/ kg.bw) attenuated the cardiotoxicity of DOX, improved the cardiac enzymes, lipid profile, reduced the urinary 8OHdG and prohibited the depletion of endogenous antioxidants and suppressed lipid peroxidation (MDA). Moreover, AJDAE enhanced DNA integrity. Histological findings showed that AJDAE (0.75 & 1.5 gm/ kg.bw) administration reduced cardiomyocytes alterations, congestion, edema and the intense cellular stress exerted on myocardial fibers as well as restored the cardiomyocytes architecture. Our data showed that AJDAE obviously resulted in protective effects against DOX-induced cardiotoxicity in rat’s heart. It can be concluded that Ajwa date offers a considerable protection against DOX-induced cardiotoxicity.

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Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats

Hormone Molecular Biology and Clinical Investigation ◽

10.1515/hmbci-2014-0041 ◽

2015 ◽

Vol 21 (3) ◽

Cited By ~ 6

Author(s):

María J. Ríos-Lugo ◽

Vanesa Jiménez-Ortega ◽

Pilar Cano-Barquilla ◽

Pilar Fernández Mateos ◽

Eduardo J. Spinedi ◽

...

Keyword(s):

Gene Expression ◽

Body Weight ◽

Feeding Behavior ◽

High Fat Diet ◽

Control Diet ◽

Male Rats ◽

Mrna Levels ◽

High Fat ◽

Medial Basal Hypothalamus ◽

Group I

AbstractPrevious studies indicate that the administration of melatonin caused body weight and abdominal visceral fat reductions in rodent models of hyperadiposity. The objective of the present study performed in high-fat fed rats was to evaluate the activity of melatonin on gene expression of some medial basal hypothalamus (MBH) signals involved in feeding behavior regulation, including neuropeptide Y (NPY), proopiomelanocortin (POMC), prolactin-releasing peptide (PrRP), leptin- and insulin-receptors (R) and insulin-R substrate (IRS)-1 and -2. Blood levels of leptin and adiponectin were also measured.Adult Wistar male rats were divided into four groups (n=16 per group): (i) control diet (3% fat); (ii) high-fat (35%) diet; (iii) high-fat diet+melatonin; (iv) control diet+melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions: (a) tap water; (b) 25 μg/mL of melatonin.After 10 weeks, the high-fat fed rats showed augmented MBH mRNA levels of NPY, leptin-R, PrRP, insulin-R, IRS-1 and IRS-2. The concomitant administration of melatonin counteracted this increase. Feeding of rats with a high-fat diet augmented expression of the MBH POMC gene through an effect insensitive to melatonin treatment. The augmented levels of circulating leptin and adiponectin seen in high-fat fed rats were counteracted by melatonin as was the augmented body weight: melatonin significantly attenuated a body weight increase in high-fat fed rats without affecting chow or water consumption. Melatonin augmented plasma leptin and adiponectin in control rats.The results indicate that an effect on gene expression of feeding behavior signals at the central nervous system (CNS) may complement a peripheral rise of the energy expenditure produced by melatonin to decrease body weight in high-fat fed rats.

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Evaluation of antihyperlipidemic and antioxidant activity of Rubia Cordifolia Linn.

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i1.2216 ◽

2019 ◽

Vol 9 (1) ◽

pp. 211-215

Author(s):

Priyanka Kumari ◽

Satish Kumar Sharma ◽

Snigdha Tiwari

Keyword(s):

Aqueous Extract ◽

High Fat Diet ◽

Low Dose ◽

Ethanolic Extract ◽

High Dose ◽

Root Extract ◽

High Fat ◽

Rubia Cordifolia ◽

Group I ◽

Aqueous Root Extract

Hyperlipidemia is major problem among population those, who have sedentary life style. The effects of ethanolic and aqueous root extract of Rubiacordifoliain experimentally induced hyperlipidemic rats were investigated. 42 wistar rats of both sexes were used for the study. The animals were completely randomized into seven groups comprising 6 animals each. The groups were treated as follows: Group I: normal diet (ND); Group II: HFD ( Vanaspati ghee + coconut oil mixture in ratio of 3:2 at 10 ml/kg/day); Group III: HFD + Atorvastatin (10 mg/kg/day); Group IV: HFD+ ethanolic extract of roots of Rubia cordifolia low dose(200 mg/kg/day); Group V: HFD + ethanolic extract of roots of Rubia cordifolia high dose (400 mg/kg/day); Group VI: HFD + aqueous extract of roots of Rubia cordifolia low dose (200 mg/kg/day); Group VII: HFD + aqueous extract of roots of Rubia cordifolia high dose (400 mg/kg/day). Hypercholesterolemia was induced by feeding the animals with high fat diet for 21 days before administration of the extract. After 21thday of feeding, administration of extract lasted for 14 days. Preliminary phytochemical screening revealed that the ethanolic and aqueous root extract of Rubia cordifolia contains glycosides, triterpenoids, saponins, alkaloids and flavonoids. Overall, findings from the present study suggest that the antihyperlipidemic activity observed with Atorvastatin (10mg/kg oral) and the ethanolic extracts of Rubia cordifolia (400mg/kg) showed better activity thanaqueous extracts of Rubia cordifolia (200mg/kg). Keywords: Rubia cordifolia, body weight, high fat diet, serum lipid, anti-hyperlipidemic activity.

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