scholarly journals Anatomical Studies of the Foliar Epidermis and Petiole of some Lannea Species in Jos, Plateau State, Nigeria

2021 ◽  
pp. 41-55
Author(s):  
Ogochukwu E. Okanume ◽  
Lynda C. Ebelebe ◽  
O. A. Oso
Keyword(s):  
Species Identification ◽  
Light Microscope ◽  
Cell Shape ◽  
Plant Science ◽  
Vascular Bundles ◽  
Anticlinal Wall ◽  
Anatomical Studies ◽  
Plateau State ◽  
Epidermal Morphology ◽  
Distinguishing Character

Aims: Leaf epidermal morphology and petiole anatomy of seven Lannea species namely; Lannea velutina, Lannea kerstingii, Lannea egregia, Lannea schimperii, Lannea acida, Lannea microcarpa, and Lannea edulis in Nigeria were investigated to provide new taxonomic characters that could help in proper identification and delimitation of the taxa. Place and Duration of Study: The study was carried out in the Department of Plant Science and Biotechnology, University of Jos, Nigeria between August 2020 and March 2021. Methodology: Transverse sections of petioles were cut using a rotary microtome and epidermal peels were made. Staining was done using Safranin and slides observed using a light microscope. Results: Stomatal distribution was hypostomatic except for L. velutina and L. edulis with amphistomatic distribution. Three stomata types are found; paracytic occurring in L. velutina, L. edulis and L. egregia, cyclocytic in L. schimperii and L. microcarpa and anomocytic in L. kerstingii and L. acida. Stomata index vary among taxa with L. egregia and L. acida having the highest (22.43%) and lowest (12.17%) respectively. The epidermal cell shape was polygonal in all the species and anticlinal wall patterns were straight to slightly curved. Unicellular/multicellular uniseriate trichomes were observed in L. schimperii, L. egregia and L. kerstingii distinguishing them from other species. Petiole outlines were circular to oval, epidermis was uniseriate and vascular bundles are collateral. Presence of continuous sclerenchyma rings surrounding the vascular bundles in L. kerstingii and L. microcarpa serve as a distinguishing character. Other distinguishing characters are presence of crystals, druses and phenolic idioblasts. A taxonomic key was produced using the anatomical character as an aid to the identification of the species. Conclusion: Variations observed in the stomata types, petiole outline, petiole vasculature type, trichome type and stomata index could be employed for species identification and delimitation.

scholarly journals Comparative foliar epidermal morphology of Isolona Engl. (Annonaceae)

2020 ◽  
Vol 12 (3) ◽  
pp. 637-645
Author(s):  
Sunday A. ADENIRAN ◽  
Akeem B. KADIRI ◽  
JAMES D. OLOWOKUDEJO
Keyword(s):  
Epidermal Cell ◽  
Light Microscope ◽  
Glandular Trichome ◽  
Anticlinal Wall ◽  
Effective Utilization ◽  
Cell Shapes ◽  
Herbal Medicinal ◽  
Generic Constant ◽  
Epidermal Morphology

A qualitative micromorphological assessment of the seven species of Isolona occurring in Nigeria and the Cameroons was undertaken with the aid of light microscope. The stomatal cells are particularly useful, providing stable characters which can be reliably employed in distinguishing the species. Hypostomatic leaves and paracytic stomatal type are generic constant. Possession of only paracytic stomata is characteristic of I. dewevrei whereas other species may have in addition another type such as 1+2 laterocytic stomatal type found only in I. zenkeri and presence of brachyparacytic stomata which shows relatedness of I. campanulata, I. congolana, I. hexaloba, I. pleurocarpus and I. thonneri. The epidermal surfaces appeared glabrous but an indication of hairs is shown by the presence of a glandular trichome base only in I. hexaloba. The epidermal cell characters such as epidermal cell shapes and anticlinal wall patterns seem to intergrade and they are not as definite as the stomatal cells. However, a combination of these features will be helpful in defining the species better and their leaf fragments can be differentiated based on the various characters studied for effective utilization in herbal medicinal research.

scholarly journals Monocotyledonous vascular bundles characterized by image analysis and pattern recognition

2018 ◽  
Author(s):  
Juliana Virginio da Silva ◽  
Cesar Comin ◽  
Luciano da Fontoura Costa
Keyword(s):  
Pattern Recognition ◽  
Image Analysis ◽  
Comparative Study ◽  
Spatial Scale ◽  
Species Identification ◽  
Cross Sections ◽  
Plant Science ◽  
Equivalent Diameter ◽  
Vascular Bundles ◽  
The Comparative Study

Recent advances in image analysis and pattern recognition have paved the way to several developments in plant science. In the present work, we report the comparative study, by using the aforementioned approaches, of vascular bundles of Dracaena marginata. More specifically, we used 33 measurements related to shape, density and regularity of imaged cross-sections of the stem. By using individual, pairwise and PCA projections of the adopted measurements, we were able to find the combinations of measurements leading to the best separation between the considered tissues. In particular, the best separation was obtained for entropy taken at a particular spatial scale combined with the equivalent diameter. The reported developments open several perspectives for applications in content-based retrieval, diagnosis, and species identification.

Reassessing species boundaries in the Syagrus glaucescens complex (Arecaceae) using leaf anatomy

Botany ◽  
2021 ◽  
pp. 379-387
Author(s):  
D.H.T. Firmo ◽  
S.A. Santos ◽  
M.E.M.P. Perez ◽  
P. Soffiatti ◽  
B.F. Sant’Anna-Santos
Keyword(s):  
Species Identification ◽  
Leaf Anatomy ◽  
Vascular System ◽  
Identification Key ◽  
Species Boundaries ◽  
Vascular Bundles ◽  
Geographical Isolation ◽  
Species Occurrence ◽  
Species Diagnosis ◽  
High Degree

The Syagrus glaucescens complex comprises three species: Syagrus glaucescens Glaz. ex Becc., Syagrus duartei Glassman, and Syagrus evansiana Noblick. Recently, a new population of S. evansiana that possesses a high degree of endemism was reported in the Serra do Cabral mountain. Here we intend to study the leaf anatomy of the S. glaucescens complex and confirm whether this newly found population (from now on called Syagrus aff. evansiana) belongs to S. evansiana or not. Specimens were collected to investigate their leaf anatomy, which showed distinct differences between S. aff. evansiana and S. evansiana. The midrib anatomy revealed novelties for the S. glauscecens complex, proving useful for species diagnosis. Features such as accessory vascular bundles around the vascular system of the midrib and the number of collateral bundles are diagnostic for species identification. In addition, morphological and anatomical analyses indicated a correlation with the species occurrence. We found greater similarity between S. glaucescens and S. duartei, while S. evansiana and S. aff. evansiana are more alike. Here, we propose a new identification key based only on the leaf anatomy. Despite their morphological similarities, S. aff. evansiana and S. evansiana presented differences in leaf anatomy, which — when associated with their geographical isolation — suggests a fourth taxon in the complex.

Comparative chemotaxonomic investigations on amaranthus hybridus l. and Amaranthus spinosus L.

2021 ◽  
Vol 20 (1) ◽  
pp. 91-100
Author(s):  
C. Wahua ◽  
J. Nwikiri
Keyword(s):  
Niger Delta ◽  
Vascular System ◽  
Vascular Bundles ◽  
Stomatal Index ◽  
Amaranthus Hybridus ◽  
Anatomical Studies ◽  
Amaranthus Spinosus ◽  
The Family ◽  
Phytochemical Studies ◽  
Key Words Morphology

The present study is set to investigate the comparative chemotaxonomic investigations on Amaranthus hybridus L. and Amaranthus spinosus L. which belong to the family Amaranthaceae. They are dicots pre-dominantly found in the Niger Delta Tropics, Nigeria. The species are annual erect herbs with flower inflorescences as elongated spikes which are mostly paniculate occurring at ends of branches in globose fashion in axils of leaves.The nodes often have pair of axillary spines. Flowers are small, greenish with male ones at the top while the female ones below the clusters and stem is greenish but often reddish with one-seeded capsule as fruit in Amaranthus spinosus which attains up to 80 ± 20cm in height whereas A. hybridus differ in absence of a pair of axillary spines, the stems are greenish or slightly pinkish which grows up to 100 ± 10cm in height. A. hybridus is more of a vegetable and has alternate phyllotaxi and narrow cuneate base. Fruits from both species are circumscissile capsules and their inflorescences are terminal racemes positioned at their axils with female perianth segments of five. Epidermal studies revealed amphistomatic stomata which is anisocytic  type for both species. The stomatal index for A. spinosus adaxial foliar epidermis is 20% and the abaxial 20% whereas for A. hybridus adaxial is 20% and abaxial foliar stomatal index of 20%. Anatomical studies revealed open vascular system, collenchyma dominating the hypodermis while parenchyma occupied the general cortex and pith regions. A. hybridus has more vascular bundles and trichomes, and wider pith than A. spinosus. Phytochemical studies showed the presence of tannins, saponins, alkaloids, and flavonoids are present in A. spinosus while alkaloids were absent only in A. hybridus. This may be the reason why A. spinosus is used more in tradomedicine than A.hybridus which served more as vegetable. Key Words: Morphology, Anatomy, Phytochemistry, Amaranthus, Amaranthaceae

scholarly journals Systematic significance of stipe anatomy of Pteris L. (Pteridaceae) from Southwestern Nigeria

2020 ◽  
Vol 12 (1) ◽  
pp. 1-13
Author(s):  
Rachael A. BAMIGBOYE ◽  
Fatai A. OLOYEDE ◽  
Akinwumi J. AKINLOYE
Keyword(s):  
Alcian Blue ◽  
Light Microscope ◽  
Vascular Bundles ◽  
Southwestern Nigeria ◽  
Systematic Significance ◽  
Safranin O

Stipe anatomy of seven Pteris species collected from various locations in the Southwestern Nigeria was investigated. This was with a view to identify characters of the stipe anatomy that are important in delimiting the species. Transverse sections (TS) of stipe at proximal median and distal regions were cut with the aid of a Reichert Austria sledge microtome at 10 µ thickness. Sections were stained in Safranin O for five minutes, rinsed in three successive changes of water and counterstained in Alcian blue for five minutes. The counterstained sections were rinsed in three changes of water, and then treated in serial grades of alcohol. Observation of prepared slides was made under light microscope. Based on the shape of the vascular bundles, the Pteris species fall into three categories   which are inverted Omega, V or U shapes. The adaxial grooves varied from proximal to distal regions in all the species except in P. ensiformis where it was uniform throughout the regions.

scholarly journals Anatomical Studies on Several Species of Heliotropium L. in Iran

2011 ◽  
Vol 3 (4) ◽  
pp. 35-43 ◽  
Author(s):  
Maryam ABBASI ◽  
Farideh ATTAR ◽  
Farrokh GHAHREMANI NEJAD
Keyword(s):  
Endemic Species ◽  
Arid Regions ◽  
Vascular Bundles ◽  
Anatomical Studies ◽  
Blade Number ◽  
Centre Of Origin ◽  
Adaptive Value ◽  
Anatomical Characters ◽  
Warm Temperate ◽  
Independent Species

Heliotropium spp. is distributed worldwide mainly in tropical and subtropical regions, with dry and warm temperate to semi-arid regions so that Southwest and center of Asia have considered as the main centre of origin and diversity of Heliotropium genus. Iran, with 32 species and 14 (sub) endemic species, has the highest diversity in the world followed by Pakistan and Turkey with 15 species and only one endemic species and the Arabian Peninsula with 15 species and three endemic species are in the next ranks. In order to anatomical studies on Heliotropium, twelve species of this genus were selected from different regions of Iran. The selected species included: H. bacciferum Forssk., H. ramossisimum BGE., H. brevilimb Boiss., H. transoxanum BGE., H. dasycarpum Ledeb, H. dyginum Forssk., H. aucheri Dc., H. carmanicum BGE. As perennial group and H. ellipticum Ledeb., H. lasiocarpum Fisch., H. suaveolens M.B. as annual group. In order to add more data to leaf anatomy characters, evaluating of systematic relevance and/or adaptive value of the morphological and anatomical diversity we have studied 24 anatomical characters in theses 12 species. For example shape and vascular bundles of main midrib, type of parenchyma cells located under lower epidermis of midrib, distance between vascular bundles and lower or upper epidermis, angle of between two parts of blade, number of cellular layers in lower or upper mesophylla, length of upper and lower mesophylla, type of cell wall in lower and upper mesophylla and thickness of lamina were investigated in this study. In order to this present obtained H. aucheri can be separated from H. carmanicum in H. aucheri subsp. carmanicum. It can be conclude that two species H. aucheri and H. carmanicum are independent species and can accept H. transoxanum as a sub group of H. dasycarpum.

scholarly journals Taxonomic significance of some leaf anatomical features of the species of Annona L. (annonaceae) from Nigeria

2020 ◽  
Vol 22 (1) ◽  
pp. 101-112
Author(s):  
S.A. Adeniran ◽  
A.B. Kadiri ◽  
J.D. Olowokudejo
Keyword(s):  
Vascular Bundles ◽  
Taxonomic Significance ◽  
Anticlinal Wall ◽  
Abaxial Surface ◽  
Anatomical Features ◽  
Qualitative And Quantitative ◽  
Dichotomous Key ◽  
The Mean ◽  
Generic Constant ◽  
Existing Data

A comparative study of the some leaf anatomical features of four species of Annona occurring in Nigerian was undertaken with the aid of light microscope. The four foliar structures (epidermis, petiole, midrib and lamina architecture) studied revealed useful characters which support recognition of the species. A combination of these features has been used to prepare an artificial indented dichotomous key for identifying the  species. The generic constant features encountered included hypostomata, paracytic stomatal type, linear nerves endings, uneven midrib outline, and centrally located vascular bundles in the petiole and midrib. However, the most reliable distinguishing characters found across the species included presence of brachyparacytic stomata in A. reticulata, presence of trichomes on the midrib in A. senegalensis, absence of druses on the abaxial surface in A. muricata and A. squamosa, a thick pitted anticlinal walls on the surfaces of A. muricata and consistent polygonal areola shape in A. squamosa. The overlapping characters which also justify the closeness of the species and their grouping in a genus were recorded in both the qualitative and quantitative features. Prominent among them are the mean stomatal width which is about 1.0 μm in all species, nerve endings within the areole which varies between 1-2, U- or V-shaped midrib on the adaxial surface and straight to curved anticlinal wall pattern. The significance of these observations is discussed in updating the existing data in the genus. Keywords: Epidermis, Microscopy, Midrib, Petiole,   Systematic

Morpho-anatomy of the cypselae of native species of Mutisieae (Asteraceae) from Mexico

Phytotaxa ◽  
2020 ◽  
Vol 436 (1) ◽  
pp. 1-20
Author(s):  
ROSARIO REDONDA-MARTÍNEZ ◽  
TERESA TERRAZAS ◽  
ALICIA ROJAS-LEAL
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Native Species ◽  
Vascular Bundles ◽  
Anticlinal Wall ◽  
Periclinal Wall ◽  
Mexican Species ◽  
Anatomical Trait ◽  
Scanning Electron

The aims of this study were to describe and illustrate cypselae of 15 Mexican species of Mutisieae from observations using optical and scanning electron microscopy. The cypselae are heteromorphic with pilose, sericeous or glandular indumentum. The primary sculpture of the surface has two microstructural patterns: reticulate and plicate. The secondary sculpture is striate in 13 taxa, but in two species, Chaptalia estribensis and C. mexicana, differences were found in the secondary sculpture of the external and internal cypselae. The most distinctive anatomical trait is in the epicarp, which has rectangular or square-shaped cells that have a convex periclinal wall in most species of Chaptalia. The mesocarp has vascular bundles with fibers in all species of Chaptalia, but fibers are absent in Adenocaulon, Gerbera and Leibnitzia. In addition, the cells of the mesocarp inner layer have either thick walls or only an anticlinal wall in nine species of Chaptalia. The micromorphological characteristics of the primary or secondary sculpture of the surface, the type of trichomes and the variation they present have taxonomical value for recognizing closely-related taxa, whereas anatomical traits of the mesocarp distinguish Adenocaulon, Gerbera and Leibnitzia from most Chaptalia species.

scholarly journals Morphological and Anatomical Studies of Hippocrepis L. Genus in Turkey

2019 ◽  
Vol 37 ◽  
Author(s):  
K. YETISEN ◽  
C. ÖZDEMIR
Keyword(s):  
Cross Section ◽  
Vascular Bundle ◽  
The Other ◽  
Vascular Bundles ◽  
Stem Anatomy ◽  
Anatomical Studies ◽  
Anatomical Features

ABSTRACT: In this study, the morphological and anatomical features were investigated of three taxon of Hippocrepis L. species which spreading naturally in Turkey. In the morphological part of the study, H. unisiliquosa subsp. unisiliquosa’s stem is erect or decumbent. The species H. ciliata’s Willd. stem is erect. H. multisiliquosa’s L. stem is decumbent. The fruit of H. multisiliquosa is much more convoluted than the other two taxa. There are cilia on the fruit of H. ciliata, but there are not found any cilia the other two taxa. The stem anatomy of all the studied taxa is hexagonal. In the stem cross section of H. ciliata 12-14 vascular bundle are found, H. unisiliquosa subsp. unisiliquosa 12-15 and H. multisiliquosa 12-13. Leaf vascular bundles are arranged regularly, H. unisiliquosa subsp. unisiliquosa have 18-20, H. ciliata have 6-8, H. multisiliquosa have 9-13 vascular bundles.

scholarly journals Scape anatomical studies in populations of Urginea indica Kunth. Liliaceae

2013 ◽  
Vol 5 (1) ◽  
pp. 179-189
Author(s):  
P. Prathima Rao ◽  
M. N. Shiva Kameshwari ◽  
K. J. Tharasaraswathi
Keyword(s):  
Cross Sections ◽  
Vascular Bundles ◽  
Anatomical Studies ◽  
Inflorescence Axis ◽  
Mediterranean Regions ◽  
Ecological Habitat ◽  
Xylem Elements ◽  
Different Populations ◽  
Urginea Indica ◽  
First Time

The current study presents investigations on the scape anatomical characteristics of Urginea indica population, a geophyte growing in India, Africa and Mediterranean regions. For scape anatomical studies transverse sections of inflorescence axis were taken and studied for the first time. The twelve different population studied revealed that outer most epidermis of inflorescence is covered by thick cuticle. Cortex is differentiated into 3 regions outer chlorenchyma, middle collenchyma and inner parenchyma. But the shape and number of rows of cells vary in different populations collected from various localities of Karnataka. Vascular bundles are arranged in 3 rows, 2 rows in few populations and number. of vascular bundles vary. Larger bundles varies from 5-8, medium 8-12 and smaller bundles 5 to 21 in number. Xylem elements are uniseriate and biseriate in few. Some populations show Myelin structures and cell inclusions, based on their ecological habitat. The distinctions among cross sections of scapes are evident and our findings offer a comprehensive study using anatomical traits for delimitation and diagnosing populations of U.indica providing a platform for further taxonomic investigations.

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