scholarly journals Amylolytic Potential of Oleaginous Yeast in Sago Processing Wastewater (SWW) under Submerged Fermentation

Author(s):  
Kiruthika Thangavelu ◽  
Pugalendhi Sundararaju ◽  
Naganandhini Srinivasan ◽  
Sivakumar Uthandi

Sago processing wastewater was assessed for their suitability as growth substrates using oleaginous yeasts, for the production of a useful enzyme (amylase) under submerged fermentation (SmF). Sago wastewater (pH was adjusted to 6) containing starch concentration (10% w/v) were inoculated with yeast strain and incubated at 30ºC for 10 d in an incubator shaker (150 rpm). The results of the amylase activity of oleaginous yeast and in its substratum SWW were compared with the different processing wastes (potato peel, banana peel, cassava peel, corn residue, rice husk, wheat bran, yam peel and barley husk) and oleaginous yeasts (Rhodotorula mucilaginosa, Saccharomyces pastorianus, Lipomyces starkeyi and Rhodotorula glutinis). Compared to other oleaginous yeast, our yeast strain found to produce higher amylase activity of 1.51 IU mL-1. Furthermore, SWW produced more amylase activity than the other compared wastes. This research finding illustrates the environmental friendly and alternate use of sago processing wastewater, towards their valorization as substrates for valuable enzymes and chemical production.

2007 ◽  
Vol 6 (18) ◽  
pp. 2130-2134 ◽  
Author(s):  
Dai Chuan chao ◽  
Tao Jie ◽  
Xie Feng ◽  
Dai Yi jun ◽  
Zhao Mo

AMB Express ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Bruno Vasconcelos ◽  
José Carlos Teixeira ◽  
Giuliano Dragone ◽  
José António Teixeira

2020 ◽  
Vol 38 (8) ◽  
pp. 873-887 ◽  
Author(s):  
Rachapudi Venkata Sreeharsha ◽  
S. Venkata Mohan

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
D. Paul ◽  
Z. Magbanua ◽  
M. Arick ◽  
T. French ◽  
S. M. Bridges ◽  
...  

2020 ◽  
Vol 28 (4) ◽  
pp. 225-233
Author(s):  
Liliia Vasina ◽  
Ivanna Kraievska ◽  
Oleksii Khudyi ◽  
Lidiia Khuda ◽  
Larysa Cheban

Abstract Freshwater zooplankton, which is commonly used as a starting fish feed, has a low content of essential compounds such as carotenoids. The possibility of increasing the productivity of carotenogenic yeast, Rhodotorula glutinis, for further bioencapsulation of zooplankton Daphnia magna is shown. An association of a UV irradiated yeast strain with lactic acid bacteria was cultivated for this purpose. This permitted intensifying the carotenogenesis of yeast, and the content of â-carotene increased by 1.7 times, and that of torularodine by 2.3 times compared with the native monoculture. The use of the association of microorganisms as a feed substrate in the cultivation of Daphnia magna provided both an increase in the carotenoid content in the investigated crustaceans more than 8 times, and their biomass by 2.5 times compared with the standard feed of Saccharomyces cerevisiae.


2021 ◽  
Vol 25 (1) ◽  
pp. 1075-1085
Author(s):  
Svetlana Raita ◽  
Kriss Spalvins ◽  
Evalds Raits ◽  
Inese Silicka ◽  
Dagnija Blumberga

Abstract The present study has investigated the effect of oil rich medium supplementation with polysorbates Tween 20, 40 and 80, for the cultivation of red oleaginous yeast Rhodotorula glutinis. R. glutinis has been cultivated in yeast extract peptone glucose modified broth (mYPG) supplemented with 2 % of waste cooking rapeseed oil and three polysorbate types with 0.5 %, 1 %, 2%, 3 %, 4 %, 5 %, 6 %, 7 %, 8 %, 9 % and 10 % concentration each. Yeast biomass was measured by the thermogravimetric method at 105 °C each day during 7-day experiment. The oil rich medium supplementation with Tween 20, 40 and 80 at concentrations ranging from 2 % to 10 % significantly increased the biomass of R. glutinis. All three types of the studied polysorbates with 0.5 % and 1 % concentration, did not affect yeast growth and the dry biomass – results were similar to the control sample without polysorbate addition. Between the three types of polysorbates, Tween 20 was selected as the preferable for R. glutinis cultivation with an optimal concentration of 2 %. Cultivation of R. glutinis in oil rich medium with polysorbates Tween 20, 40 and 80, supplementation up to 10 % concentration did not have had an inhibitory effect on the biomass growth.


2021 ◽  
Author(s):  
Zhi-Peng Wang ◽  
Jing-Run Ye ◽  
Yan Ma ◽  
Xin-Yue Zhang ◽  
Hai-ying Wang ◽  
...  

Abstract BackgroundRaw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. ResultsIn this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. ConclusionsThis is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess


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