Serum Levels of Adenosine Deaminase and Insulin in Type 2 Diabetes Mellitus

Aim: To estimate the levels of serum adenosine deaminase and insulin in patients with type 2 diabetes mellitus and healthy subjects and to find the correlation between serum adenosine deaminase and serum insulin in type 2 diabetes mellitus. Study Design: Case-control study. Place and Duration of the Study: Department of Biochemistry and Department of Medicine, Regional Institute of Medical Sciences (RIMS), Imphal, Manipur between October 2017 to September 2019. Methodology: 40 cases of type 2 diabetes mellitus were recruited from the Medicine Outpatient Department, RIMS and 40 healthy controls of age and sex-matched were recruited from those who came for a routine health check-up. Serum adenosine deaminase was measured by the calorimetry method and serum insulin was measured by ELISA method. The data were analyzed using statistical tools like Chi-square test, Independent sample t-test, Pearson’s Correlation through SPSS 21.0. Results: Mean serum adenosine deaminase was (38.97±8.853)U/L in cases and (20.05±5.309)U/L in controls and it was statistically significant (P<0.001). Mean serum insulin in cases was found to be (18.09±5.554) μIU whereas in controls, it was (9.06±2.509) μIU which is statistically significant (p<0.001). Serum adenosine deaminase and insulin were found to be positively correlated to each other (r= 0.956, p<0.001). Conclusion: Serum adenosine deaminase and serum insulin were significantly increased in type 2 diabetes mellitus and positively correlated to each other. Serum adenosine deaminase may be used as a prognostic marker for the pathogenesis of type 2 diabetes mellitus.

Download Full-text

The Relationship Of The Level Of Family Knowledge And Efforts To Prevent Complications Of Diabetes Mellitus Tipe 2 In The Working Area Of Babakan Public Health Center District Of Cirebon

Jurnal Kesehatan Mahardika ◽

10.54867/jkm.v8i1.17 ◽

2021 ◽

Vol 8 (1) ◽

pp. 14-19

Author(s):

Lukatul Khoiriyah ◽

Ramli Effendi ◽

Thia Oktiany

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Health Center ◽

P Value ◽

Chi Square ◽

Work Area ◽

Chi Square Test ◽

The Relationship

The level of family knowledge is a very important factor in the actions of families of people with Diabetes Mellitus, behavior based on knowledge will be easier to carry out than those not based on knowledge. Diabetes Mellitus is a disease that requires proper and immediate treatment efforts because it can cause complications such as heart disease, kidney failure, and damage to the nervous system. One way to overcome the effects of Type 2 Diabetes Mellitus is by applying a diet. This study aims to determine the relationship between the level of family knowledge with efforts to prevent complications of type 2 diabetes mellitus in the work area of Babakan Puskesmas, Cirebon Regency. This research method uses descriptive correlational, this research uses cross section research. The population is all families with type 2 Diabetes Mellitus in the work area of Babakan Puskesmas in Cirebon Regency as many as 144 respondents and sampling using Purposive Sampling obtained 60 respondents. The instruments used were questionnaire sheets, and data analysis using chi-square test that is the significance value α = 0.1. The results of the above study indicate between the level of family knowledge and prevention of complications of type 2 diabetes mellitus in getting p-value = 0.04, because the p-value of 0.04 <0.1 then Ho is rejected and Ha is accepted which means there is a relationship between the level of family knowledge and prevention of complications of type 2 diabetes mellitus in the work area of Babakan Health Center, Cirebon Regency.

Download Full-text

Kepatuhan dan Kualitas Hidup Pasien Diabetes Melitus Tipe 2 di Rumah Sakit di Jawa Tengah

Pharmacon Jurnal Farmasi Indonesia ◽

10.23917/pharmacon.v17i2.12281 ◽

2020 ◽

Vol 17 (2) ◽

pp. 165-173

Author(s):

Nurul Mutmainah ◽

Muhammad Al Ayubi ◽

Anggie Widagdo

Keyword(s):

Quality Of Life ◽

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Short Form ◽

Chi Square ◽

Central Java ◽

Chi Square Test

Diabetes mellitus is a chronic disease that requires treatment for a long period of time. Adherence to consuming drugs is one of the most important things in achieving treatment goals. This study aims to determine the level of adherence and quality of life and determine the relationship to the quality of life of patients with type 2 DM in hospitals in Central Java. This study uses analytic design by asking cross-sectional. Samples were taken at two hospitals in Central Java. Sampling was purposive sampling with the criteria of type 2 diabetes mellitus patients who are undergoing outpatient care in the hospital, have undergone therapy for at least 3 months, and can communicate well. As a measurement used the Medication Adherence Rating Scale (MARS) questionnaire while the quality of life measure used the Short Form 36 (SF-36) questionnaire. Score data received and quality of life that has been obtained was processed with the SPSS (Product Statistics and Service Solutions) chi-square test. Research results in 200 respondents found 9 people (4.5%) had low adherence, 46 people (23%) were moderately related and 145 people (72.5%) have high adherence. While the quality of life found 41 people (20.5%) had a poor quality of life and 159 people (79.5%) had a good quality of life. The lowest aspect of quality of life is physical limitations and the highest aspect of quality of life is mental health. From the results of the analysis on the chi square test, the p value was obtained: 0.00. This shows that there is a significant relationship between adherence and quality of life in patients with Type 2 diabetes mellitus at the hospitals and a Prevalence Ratio value of 23.47.

Download Full-text

HUBUNGAN POLA MAKAN TERHADAP STATUS GIZI PASIEN RAWAT JALAN DIABETES MELITUS TIPE 2 DI WILAYAH KERJA RUMAH SAKIT GRANDMED LUBUK PAKAM

JURNAL KESEHATAN MASYARAKAT & GIZI (JKG) ◽

10.35451/jkg.v3i2.660 ◽

2021 ◽

Vol 3 (2) ◽

pp. 168-171

Author(s):

Raini Panjaitan ◽

Reno Irwanto ◽

Andreais Boffil Cholilullah ◽

Salmi Angraini

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Nutrient Status ◽

P Value ◽

Chi Square ◽

Cross Sectional ◽

Chi Square Test ◽

Cross Sectional Design

Diabetes mellitus is a chronic disease characterized by hyperlycemia. Risk factors for type 2 diabetes mellitus due to intake of carbohydrates, fats and protein. This study aims to see the relationship between diet in the nutrient status of type 2 diabetes mellitus in out patient in Grandmed Hospital Lubuk Pakam. This research method is an observational description with cross-sectional design. The sample is patients type 2 diabetes mellitus in out patient in Grandmed Hospital Lubuk Pakam. The sample is 30 people. The instruments are Food Recall 24 Hours and analyzed by nutrition app Nutri Survey. The data analysis used in this study was the Chi-Square test (95% CI) From the result of this research showed that 44,6% of patients with excess carbohydrate intake with a p value of 0.000 ≤ α (0,05). this research showed that 49,0% of patients with excess fat intake with a p value of 0.000 ≤ α (0,05). this research showed that 38,0% of patients with excess protein intake with a p value of 0.000 ≤ α (0,05). The conclusion are that there is significant relationship between diet in the nutrient status of type 2 diabetes mellitus in out patient in Grandmed Hospital Lubuk Pakam and type 2 diabetes mellitus in out patient nutrient status characteristics categorized as obesity.

Download Full-text

Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia

Jurnal Teknologi Laboratorium ◽

10.29238/teknolabjournal.v9i2.235 ◽

2020 ◽

Vol 9 (2) ◽

pp. 186-191

Author(s):

Miftahul Mushlih ◽

Fitri Kumala Sari ◽

Hindah Sabrina Amin ◽

Siti Asriani Iknan

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Scoring Method ◽

Chi Square ◽

Cross Sectional ◽

Chi Square Test ◽

Random Amplified Polymorphism Dna ◽

Rapd Method

T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.

Download Full-text

Vascular Endothelial Growth Factor (VEGF) -460C/T (rs833061) Gene Polymorphism as a Risk Factor for Diabetic Retinopathy in Type 2 Diabetes Mellitus in Bali

International Journal of Research and Review ◽

10.52403/ijrr.20211201 ◽

2021 ◽

Vol 8 (12) ◽

pp. 1-6

Author(s):

Astri Meliana ◽

Desak Made Wihandani ◽

Ni Nyoman Ayu Dewi

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Diabetic Retinopathy ◽

Risk Factor ◽

Gene Polymorphism ◽

Bivariate Analysis ◽

Chi Square ◽

Chi Square Test

Introduction: Diabetic retinopathy (DR) is a microvascular disorder of the retina caused by hyperglycemia in the blood vessels and is the most common complication in the eye due to diabetes mellitus (DM). The aim of this study was to determine the VEGF -460C/T gene polymorphism as a risk factor for diabetic retinopathy in T2DM patients in Bali. Materials and Methods: The design of this study was case-control with 27 cases of type 2 DM with DR and 29 cases without RD as controls. The VEGF-460C/T polymorphism in DNA was detected using PCR and DNA sequencing at rs833061 to see the distribution of the C/T allele variation. Data were analyzed using chi-square test. Results: Based on bivariate analysis comparing homozygous TT genotype variants, heterozygous CT and wild-type CC in this study, no significant relationship was found with the incidence of DR (p=0.742). Conclusion: Polymorphism of the VEGF-460C/T gene (rs833061) can be concluded as an irrelevant factor with the risk of developing DR in type 2 diabetes mellitus patients in Bali. Keywords: VEGF -460C/T, Diabetes Mellitus, Polymorphism, Risk Factors.

Download Full-text

Association analysis of SOCS3, JAK2 and STAT3 gene polymorphisms and genetic susceptibility to type 2 diabetes mellitus in Chinese population

Diabetology & Metabolic Syndrome ◽

10.1186/s13098-021-00774-w ◽

2022 ◽

Vol 14 (1) ◽

Author(s):

Yang Zhang ◽

Chunwen Lin ◽

Rong Chen ◽

Ling Luo ◽

Jialu Huang ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Environmental Factors ◽

Genetic Susceptibility ◽

Frequency Distribution ◽

Chinese Population ◽

Chi Square ◽

Chi Square Test

Abstract Aim The association of polymorphisms in the three genes of SOCS3, JAK2 and STAT3 with genetic susceptibility to type 2 diabetes mellitus (T2DM) was explored, and its interaction with environmental factors such as hypertension and triglycerides was analyzed. Methods The Hardy–Weinberg balance test was used to analyze the random balance of genes in the population. The analysis of the association of SNPs with T2DM was performed using Pearson’s chi-square test. Haplotype frequency distribution, SNPs-SNPs interaction and environmental factors were analyzed by chi-square test and logistic regression. Results The genotype distribution of SNPs rs2280148 of the SOCS3 gene was statistically significant. The allele frequency distribution of SNPs (rs4969168/rs2280148) was statistically different. After covariate correction, the SOCS3 gene locus (rs4969168) showed an association with T2DM in additive model, while the rs2280148 locus showed an association with T2DM in all three models. The locus (rs10974914/rs10815157) allele and genotype frequency distribution of JAK2 were statistically significant. After covariate correction, two SNPs in the gene showed association with T2DM in both additive and recessive models. The distribution of genotype frequencies of SNPs rs1053005 locus in gene STAT3 was statistically significant between the two groups. In recessive genetic models, rs1053005 locus polymorphisms was associated with T2DM. Haplotype S3 (G G)/S 4 (G T) of the SOCS3 gene as well as haplotype J2 (A G)/J 3 (G C) of the JAK2 gene were closely associated with T2DM. There was an interaction between SNPs rs4969168 and SNPs rs2280148 in the SOCS3 gene. There was an interaction between the SOCS3, JAK2 and STAT3 genes and hypertension/triglycerides. Conclusion The SOCS3 and JAK2 genes may be associated with T2DM in the Chinese population, in which SNPs carrying the A allele (rs4969168)/G allele (rs2280148)/C allele (rs10815157) have a reduced risk of T2DM. Haplotype S3 (G G)/S 4 (G T) of the SOCS3 gene and haplotype J2 (A G)/J 3 (G C) of the JAK2 gene may be influencing factor for T2DM. The interaction between SNPs rs4969168 and SNPs rs2280148 increases the risk of T2DM. Hypertension and triglycerides may interact with SNPs of T2DM susceptibility genes.

Download Full-text

A study on diastolic dysfunction in asymptomatic type 2 diabetes mellitus with normal systolic function

International Journal of Advances in Medicine ◽

10.18203/2349-3933.ijam20192259 ◽

2019 ◽

Vol 6 (3) ◽

pp. 890

Author(s):

M. Ilamaran ◽

A. Thangadhurai ◽

P. Suresh Kumar ◽

Saranya Nagalingam

Keyword(s):

Diabetes Mellitus ◽

Heart Failure ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Diastolic Dysfunction ◽

Diastolic Heart Failure ◽

Chi Square ◽

High Incidence ◽

Chi Square Test

Background: Diastolic heart failure, otherwise called as heart failure with preserved ejection fraction, is common finding of hypertensive heart disease, but various studies report a high incidence of diastolic heart failure in patients with type 2 diabetes mellitus in spite of the absence of coronary artery heart disease and hypertension. The objectives of the study were to determine the prevalence of dysfunction of left ventricle in diastole in type 2 diabetes mellitus patients and to compare with the non-diabetic individuals with that of asymptomatic type II diabetes patients. To assess the correlation of diastolic dysfunction in diabetes with age of patients, HbA1c levels, duration of diabetes, retinopathy, autonomic neuropathy.Methods: A prospective cross sectional among them 50 patients were diabetics and 50 were non-diabetic controls. Diastolic dysfunction was measured with standard echocardiographic parameters and the results were computed with corresponding variables of the patients. All the variables and their data were analysed for percentage, mean, standard deviation ‘t’ test and chi square test. The ‘t’-test was used to study the quantitative data while chi square test was used to study the qualitative data.Results: Among the study population 60% had diastolic dysfunction and 14% had diastolic dysfunction among cases and control group respectively. Diastolic dysfunction was present among 23.3% and the 76.7% of the age group groups of less than 45 and more than 45 years of age respectively. In this study poor glycaemic status was significantly associated with diastolic dysfunction, whereas duration of diabetes, retinopathy and autonomic neuropathy were not statistically significant.Conclusions: Present study reveals moderately high incidence of diastolic dysfunction in asymptomatic diabetic; subjects and, this finding was correlated with the HBA1C levels but not with retinopathy and autonomic neuropathy.

Download Full-text

Abstract #212 Association of Serum Insulin and Urinary Albumin with Occult Coronary Artery Disease in Patients with Type-2 Diabetes Mellitus

Endocrine Practice ◽

10.1016/s1530-891x(20)47058-2 ◽

2018 ◽

Vol 24 ◽

pp. 33-34

Author(s):

Sudhir Bhandari ◽

Senior ◽

Banshi Saboo ◽

Shashank Joshi

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Coronary Artery Disease ◽

Type 2 Diabetes Mellitus ◽

Coronary Artery ◽

Serum Insulin ◽

Urinary Albumin ◽

Artery Disease

Download Full-text

Vitamin D Supplementation and Serum Levels of Magne-sium and Selenium in Type 2 Diabetes Mellitus Patients: Gender Dimorphic Changes

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000190 ◽

2014 ◽

Vol 84 (1-2) ◽

pp. 27-34 ◽

Cited By ~ 7

Author(s):

Nasser M. Al-Daghri ◽

Khalid M. Alkharfy ◽

Nasiruddin Khan ◽

Hanan A. Alfawaz ◽

Abdulrahman S. Al-Ajlan ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Vitamin D ◽

Type 2 Diabetes Mellitus ◽

Serum Levels ◽

Vitamin D Supplementation ◽

Serum Selenium ◽

Dependent Manner

The aim of our study was to evaluate the effects of vitamin D supplementation on circulating levels of magnesium and selenium in patients with type 2 diabetes mellitus (T2DM). A total of 126 adult Saudi patients (55 men and 71 women, mean age 53.6 ± 10.7 years) with controlled T2DM were randomly recruited for the study. All subjects were given vitamin D3 tablets (2000 IU/day) for six months. Follow-up mean concentrations of serum 25-hydroxyvitamin D [25-(OH) vitamin D] significantly increased in both men (34.1 ± 12.4 to 57.8 ± 17.0 nmol/L) and women (35.7 ± 13.5 to 60.1 ± 18.5 nmol/L, p < 0.001), while levels of parathyroid hormone (PTH) decreased significantly in both men (1.6 ± 0.17 to 0.96 ± 0.10 pmol/L, p = 0.003) and women (1.6 ± 0.17 to 1.0 ± 0.14 pmol/L, p = 0.02). In addition, there was a significant increase in serum levels of selenium and magnesium in men and women (p-values < 0.001 and 0.04, respectively) after follow-up. In women, a significant correlation was observed between delta change (variables at six months-variable at baseline) of serum magnesium versus high-density lipoprotein (HDL)-cholesterol (r = 0.36, p = 0.006) and fasting glucose (r = - 0.33, p = 0.01). In men, there was a significant correlation between serum selenium and triglycerides (r = 0.32, p = 0.04). Vitamin D supplementation improves serum concentrations of magnesium and selenium in a gender-dependent manner, which in turn could affect several cardiometabolic parameters such as glucose and lipids.

Download Full-text