Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia

T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.

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HUBUNGAN POLA MAKAN TERHADAP STATUS GIZI PASIEN RAWAT JALAN DIABETES MELITUS TIPE 2 DI WILAYAH KERJA RUMAH SAKIT GRANDMED LUBUK PAKAM

JURNAL KESEHATAN MASYARAKAT & GIZI (JKG) ◽

10.35451/jkg.v3i2.660 ◽

2021 ◽

Vol 3 (2) ◽

pp. 168-171

Author(s):

Raini Panjaitan ◽

Reno Irwanto ◽

Andreais Boffil Cholilullah ◽

Salmi Angraini

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Nutrient Status ◽

P Value ◽

Chi Square ◽

Cross Sectional ◽

Chi Square Test ◽

Cross Sectional Design

Diabetes mellitus is a chronic disease characterized by hyperlycemia. Risk factors for type 2 diabetes mellitus due to intake of carbohydrates, fats and protein. This study aims to see the relationship between diet in the nutrient status of type 2 diabetes mellitus in out patient in Grandmed Hospital Lubuk Pakam. This research method is an observational description with cross-sectional design. The sample is patients type 2 diabetes mellitus in out patient in Grandmed Hospital Lubuk Pakam. The sample is 30 people. The instruments are Food Recall 24 Hours and analyzed by nutrition app Nutri Survey. The data analysis used in this study was the Chi-Square test (95% CI) From the result of this research showed that 44,6% of patients with excess carbohydrate intake with a p value of 0.000 ≤ α (0,05). this research showed that 49,0% of patients with excess fat intake with a p value of 0.000 ≤ α (0,05). this research showed that 38,0% of patients with excess protein intake with a p value of 0.000 ≤ α (0,05). The conclusion are that there is significant relationship between diet in the nutrient status of type 2 diabetes mellitus in out patient in Grandmed Hospital Lubuk Pakam and type 2 diabetes mellitus in out patient nutrient status characteristics categorized as obesity.

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Correlation Analysis Study between Hearing Loss Occurence and Duration of Type 2 Diabetes Mellitus in Sultan Agung Islamic Hospital Semarang

Sains Medika ◽

10.26532/sainsmed.v6i1.338 ◽

2015 ◽

Vol 6 (1) ◽

pp. 12

Author(s):

Citta Arunika Risyudhanti ◽

Agung Sulistyanto ◽

Suparmi Suparmi

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Hearing Impairment ◽

Medical Records ◽

Pure Tone ◽

Pure Tone Audiometry ◽

Chi Square ◽

Cross Sectional

Introduction: Hearing impairment due to cochlear damage is one complication of Type 2 Diabetes Mellitus (DM2). Objective: This study aims to determine the relationship between duration of DM2 with hearing impairment.Method: The analytic observasional study with the cross sectional design using the medical record of DM2 outpatient in internal clinic of Islamic Sultan Agung Hospital Semarang and also pure tone audiometric examination’s record in Otorynolaryngology clinic of Islamic Sultan Agung Hospital Semarang.31 medical records of DM2 patients were meeting the inclusion and exclusion criteria. The data consist of 18 medical records of patients with less than6 years duration of DM2, and 13 medical records of patients with more than 6 years duration of DM2. The pure tone audiometry was used to classified the hearing impairment. The data analysis was conducted using SPSS 20.0. The correlation between the duration of DM2 and hearing impairment was analyzed using Chi Square test. The significance relation between the duration of DM type 2 and hearing impairment was analyzed using cooficient contigency, with significance of < 0.05. Result: hearing impairment’s distribution frequency on tested ears in Sultan agung Islamic Hospital Semarang were 27 ears having SNHL type (43,5%), 19 ears having CHL (30,6%) and 16 ears were normal (25,8%), p = 0,02 (p < 0,05). Conclusion: There is a weak correlation between the DM 2 duration and the incidence of hearing impairment in Sultan Agung Islamic Hospital Semarang.

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The Relationship Of The Level Of Family Knowledge And Efforts To Prevent Complications Of Diabetes Mellitus Tipe 2 In The Working Area Of Babakan Public Health Center District Of Cirebon

Jurnal Kesehatan Mahardika ◽

10.54867/jkm.v8i1.17 ◽

2021 ◽

Vol 8 (1) ◽

pp. 14-19

Author(s):

Lukatul Khoiriyah ◽

Ramli Effendi ◽

Thia Oktiany

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Health Center ◽

P Value ◽

Chi Square ◽

Work Area ◽

Chi Square Test ◽

The Relationship

The level of family knowledge is a very important factor in the actions of families of people with Diabetes Mellitus, behavior based on knowledge will be easier to carry out than those not based on knowledge. Diabetes Mellitus is a disease that requires proper and immediate treatment efforts because it can cause complications such as heart disease, kidney failure, and damage to the nervous system. One way to overcome the effects of Type 2 Diabetes Mellitus is by applying a diet. This study aims to determine the relationship between the level of family knowledge with efforts to prevent complications of type 2 diabetes mellitus in the work area of Babakan Puskesmas, Cirebon Regency. This research method uses descriptive correlational, this research uses cross section research. The population is all families with type 2 Diabetes Mellitus in the work area of Babakan Puskesmas in Cirebon Regency as many as 144 respondents and sampling using Purposive Sampling obtained 60 respondents. The instruments used were questionnaire sheets, and data analysis using chi-square test that is the significance value α = 0.1. The results of the above study indicate between the level of family knowledge and prevention of complications of type 2 diabetes mellitus in getting p-value = 0.04, because the p-value of 0.04 <0.1 then Ho is rejected and Ha is accepted which means there is a relationship between the level of family knowledge and prevention of complications of type 2 diabetes mellitus in the work area of Babakan Health Center, Cirebon Regency.

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Prevalence of Metabolic Syndrome in individuals with Type 2 Diabetes Mellitus

Revista Brasileira de Enfermagem ◽

10.1590/0034-7167-2016-0145 ◽

2017 ◽

Vol 70 (2) ◽

pp. 265-270 ◽

Cited By ~ 8

Author(s):

José Cláudio Garcia Lira Neto ◽

Mayra de Almeida Xavier ◽

José Wicto Pereira Borges ◽

Márcio Flávio Moura de Araújo ◽

Marta Maria Coelho Damasceno ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Metabolic Syndrome ◽

Type 2 Diabetes Mellitus ◽

Descriptive Analysis ◽

Cross Sectional Study ◽

Diabetic Patients ◽

Chi Square ◽

Cross Sectional

ABSTRACT Objective: to identify the prevalence of Metabolic Syndrome and its components in a population of patients with type 2 Diabetes Mellitus. Method: a cross-sectional study was conducted with 201 diabetic patients. A descriptive analysis and Chi-square and Fisher's exact tests ( p <0.05) were performed. Results: the majority of participants were females and overweight, with a mean age of 63.1 years and a low level of education, and categorized as physically inactive. Of all individuals investigated, 50.7% were diagnosed with Metabolic Syndrome and 92% had at least one of the syndrome components with values beyond those recommended. Conclusion: it is essential to take preventive actions and develop studies that help to identify the factors associated with this syndrome.

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Kepatuhan dan Kualitas Hidup Pasien Diabetes Melitus Tipe 2 di Rumah Sakit di Jawa Tengah

Pharmacon Jurnal Farmasi Indonesia ◽

10.23917/pharmacon.v17i2.12281 ◽

2020 ◽

Vol 17 (2) ◽

pp. 165-173

Author(s):

Nurul Mutmainah ◽

Muhammad Al Ayubi ◽

Anggie Widagdo

Keyword(s):

Quality Of Life ◽

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Short Form ◽

Chi Square ◽

Central Java ◽

Chi Square Test

Diabetes mellitus is a chronic disease that requires treatment for a long period of time. Adherence to consuming drugs is one of the most important things in achieving treatment goals. This study aims to determine the level of adherence and quality of life and determine the relationship to the quality of life of patients with type 2 DM in hospitals in Central Java. This study uses analytic design by asking cross-sectional. Samples were taken at two hospitals in Central Java. Sampling was purposive sampling with the criteria of type 2 diabetes mellitus patients who are undergoing outpatient care in the hospital, have undergone therapy for at least 3 months, and can communicate well. As a measurement used the Medication Adherence Rating Scale (MARS) questionnaire while the quality of life measure used the Short Form 36 (SF-36) questionnaire. Score data received and quality of life that has been obtained was processed with the SPSS (Product Statistics and Service Solutions) chi-square test. Research results in 200 respondents found 9 people (4.5%) had low adherence, 46 people (23%) were moderately related and 145 people (72.5%) have high adherence. While the quality of life found 41 people (20.5%) had a poor quality of life and 159 people (79.5%) had a good quality of life. The lowest aspect of quality of life is physical limitations and the highest aspect of quality of life is mental health. From the results of the analysis on the chi square test, the p value was obtained: 0.00. This shows that there is a significant relationship between adherence and quality of life in patients with Type 2 diabetes mellitus at the hospitals and a Prevalence Ratio value of 23.47.

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Vascular Endothelial Growth Factor (VEGF) -460C/T (rs833061) Gene Polymorphism as a Risk Factor for Diabetic Retinopathy in Type 2 Diabetes Mellitus in Bali

International Journal of Research and Review ◽

10.52403/ijrr.20211201 ◽

2021 ◽

Vol 8 (12) ◽

pp. 1-6

Author(s):

Astri Meliana ◽

Desak Made Wihandani ◽

Ni Nyoman Ayu Dewi

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Diabetic Retinopathy ◽

Risk Factor ◽

Gene Polymorphism ◽

Bivariate Analysis ◽

Chi Square ◽

Chi Square Test

Introduction: Diabetic retinopathy (DR) is a microvascular disorder of the retina caused by hyperglycemia in the blood vessels and is the most common complication in the eye due to diabetes mellitus (DM). The aim of this study was to determine the VEGF -460C/T gene polymorphism as a risk factor for diabetic retinopathy in T2DM patients in Bali. Materials and Methods: The design of this study was case-control with 27 cases of type 2 DM with DR and 29 cases without RD as controls. The VEGF-460C/T polymorphism in DNA was detected using PCR and DNA sequencing at rs833061 to see the distribution of the C/T allele variation. Data were analyzed using chi-square test. Results: Based on bivariate analysis comparing homozygous TT genotype variants, heterozygous CT and wild-type CC in this study, no significant relationship was found with the incidence of DR (p=0.742). Conclusion: Polymorphism of the VEGF-460C/T gene (rs833061) can be concluded as an irrelevant factor with the risk of developing DR in type 2 diabetes mellitus patients in Bali. Keywords: VEGF -460C/T, Diabetes Mellitus, Polymorphism, Risk Factors.

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Hubungan Aktivitas Fisik dan Kualitas Hidup Lansia Penderita Diabetes Mellitus Tipe 2

JURNAL KESEHATAN MERCUSUAR ◽

10.36984/jkm.v3i2.132 ◽

2021 ◽

Vol 3 (2) ◽

pp. 39-44

Author(s):

Eltrikanawati T. ◽

Larasuci Arini ◽

Ilma Chantika

Keyword(s):

Quality Of Life ◽

Physical Activity ◽

Diabetes Mellitus ◽

Type 2 Diabetes ◽

P Value ◽

Chi Square ◽

Cross Sectional ◽

Chi Square Test

ABSTRACT Diabetes mellitus is a non-communicable disease whose prevalence continues to increase in the world, both in developed and developing countries. Physical activity is also a major risk factor in triggering Diabetes Mellitus. This study aims to determine the relationship of physical activity with the quality of life of elderly people with type 2 diabetes mellitus. The research method used is descriptive analytic with cross sectional design. The sampling technique in this research is purposive sampling with 58 samples. The instrument used in this study was a GPAQ physical activity questionnaire and quality of life using a WHOQOL questionnaire. The statistical test used is the chi square test. The results showed the majority with low activity of 23 elderly (38.6%) and the quality of life of the majority of good elderly totaling 30 elderly (51.7%) obtained p value of 0.001 <0.05 then H0 is rejected and Ha is accepted and can be conclusions that there is a significant relationship between physical activity with the quality of life of elderly people with diabetes mellitus type II. As health workers are expected to provide education to the community, especially in the elderly about physical activity one of them is elderly exercise, because of good physical activity and regularly provide benefits for the body, especially for people with Diabetes Mellitus type 2. Keywords: Physical Activity; Quality of life; Elderly; Type 2 Diabetes Mellitus ABSTRAK Diabetes mellitus merupakan salah satu penyakit tidak menular yang prevalensinya terus mengalami peningkatan didunia, baik dinegara maju maupun negara berkembang. Aktivitas fisik merupakan faktor resiko mayor dalam memicu terjadinya Diabetes Mellitus. Tujuan penelitian adalah untuk mengetahui hubungan aktivitas fisik dengan kualitas hidup lansia penderita diabetes mellitus tipe 2. Metode penelitan yang digunakan yaitu Deskriptif Analitik dengan rangcangan cross sectional. Teknik pengambilan sampel menggunakan purposive sampling sebanyak 58 sampel. Instrumen yang digunakan adalah koesioner aktivitas fisik GPAQ dan kualitas hidup menggunakan koesioner WHOQOL. Uji statistik yang digunakan ialah chi square test. Hasil penelitian didapatkan mayoritas dengan aktivitas rendah sebanyak 23 lansia (38,6%) dan kualitas hidup lansia mayoritas baik berjumlah 30 lansia (51,7%) didapatkan nilai p value 0,001 < 0,05 maka H0 di tolak dan Ha diterima. Kesimpulannya adalah terdapat hubungan bermakna antara aktivitas fisik dengan kualitas hidup lansia penderita diabetes mellitus tipe 2 Sebagai tenaga kesehatan diharapkan dapat memberikan edukasi kepada masyarakat khususnya pada lansia mengenai aktivitas fisik salah satunya adalah senam lansia, karena aktivitas fisik yang baik dan teratur memberikan manfaat bagi tubuh, khususnya bagi penderita Diabetes Mellitus tipe 2. Kata Kunci: Aktifitas Fisik; Kualitas Hidup; Lanjut Usia; Diabetes Mellitus Tipe 2

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Association analysis of SOCS3, JAK2 and STAT3 gene polymorphisms and genetic susceptibility to type 2 diabetes mellitus in Chinese population

Diabetology & Metabolic Syndrome ◽

10.1186/s13098-021-00774-w ◽

2022 ◽

Vol 14 (1) ◽

Author(s):

Yang Zhang ◽

Chunwen Lin ◽

Rong Chen ◽

Ling Luo ◽

Jialu Huang ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Environmental Factors ◽

Genetic Susceptibility ◽

Frequency Distribution ◽

Chinese Population ◽

Chi Square ◽

Chi Square Test

Abstract Aim The association of polymorphisms in the three genes of SOCS3, JAK2 and STAT3 with genetic susceptibility to type 2 diabetes mellitus (T2DM) was explored, and its interaction with environmental factors such as hypertension and triglycerides was analyzed. Methods The Hardy–Weinberg balance test was used to analyze the random balance of genes in the population. The analysis of the association of SNPs with T2DM was performed using Pearson’s chi-square test. Haplotype frequency distribution, SNPs-SNPs interaction and environmental factors were analyzed by chi-square test and logistic regression. Results The genotype distribution of SNPs rs2280148 of the SOCS3 gene was statistically significant. The allele frequency distribution of SNPs (rs4969168/rs2280148) was statistically different. After covariate correction, the SOCS3 gene locus (rs4969168) showed an association with T2DM in additive model, while the rs2280148 locus showed an association with T2DM in all three models. The locus (rs10974914/rs10815157) allele and genotype frequency distribution of JAK2 were statistically significant. After covariate correction, two SNPs in the gene showed association with T2DM in both additive and recessive models. The distribution of genotype frequencies of SNPs rs1053005 locus in gene STAT3 was statistically significant between the two groups. In recessive genetic models, rs1053005 locus polymorphisms was associated with T2DM. Haplotype S3 (G G)/S 4 (G T) of the SOCS3 gene as well as haplotype J2 (A G)/J 3 (G C) of the JAK2 gene were closely associated with T2DM. There was an interaction between SNPs rs4969168 and SNPs rs2280148 in the SOCS3 gene. There was an interaction between the SOCS3, JAK2 and STAT3 genes and hypertension/triglycerides. Conclusion The SOCS3 and JAK2 genes may be associated with T2DM in the Chinese population, in which SNPs carrying the A allele (rs4969168)/G allele (rs2280148)/C allele (rs10815157) have a reduced risk of T2DM. Haplotype S3 (G G)/S 4 (G T) of the SOCS3 gene and haplotype J2 (A G)/J 3 (G C) of the JAK2 gene may be influencing factor for T2DM. The interaction between SNPs rs4969168 and SNPs rs2280148 increases the risk of T2DM. Hypertension and triglycerides may interact with SNPs of T2DM susceptibility genes.

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Hubungan Berbagai Faktor Risiko Terhadap Kejadian Penyakit Jantung Koroner Pada Penderita Diabetes Melitus Tipe 2

Jurnal Kesehatan Andalas ◽

10.25077/jka.v3i1.22 ◽

2014 ◽

Vol 3 (1) ◽

Author(s):

Fadma Yuliani ◽

Fadil Oenzil ◽

Detty Iryani

Keyword(s):

Diabetes Mellitus ◽

Risk Factors ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Heart Disease ◽

Smoking Habit ◽

Chi Square ◽

Cross Sectional ◽

Mortality And Morbidity

AbstrakPenyebab mortalitas dan morbiditas utama pada pasien diabetes mellitus (DM) tipe 2 adalah penyakit jantung koroner (PJK) dimana penderitanya dua sampai empat kali lebih berisiko terkena penyakit jantung dari pada non DM. Mekanisme terjadinya PJK pada DM tipe 2 dikaitkan dengan adanya aterosklerosis yang dipengaruhi oleh berbagai faktor. Penelitian ini bertujuan mengetahui hubungan berbagai faktor risiko terhadap kejadian PJK pada penderita DM tipe 2. Penelitian dilaksanakan di RSUP. Dr. M. Djamil Padang dan RS. Khusus Jantung Sumbar pada bulan Maret-Agustus 2013. Penelitian bersifat analitik dengan desain cross sectional comparative. Jumlah sampel 176 orang yang terdiri dari 88 orang penderita DM dengan PJK dan 88 orang DM tanpa PJK. Pengolahan data dilakukan dengan uji chi-square menggunakan sistem komputerisasi. Hasil Penelitian menunjukkan bahwa faktor risiko yang berhubungan dengan kejadian PJK pada penderita DM tipe 2 adalah jenis kelamin (p=0,000), lama menderita DM (p=0,043), hipertensi (p=0,007), dislipidemia (p=0,000), obesitas (p=0,023), dan merokok (p=0,000). Kesimpulan: Terdapat hubungan yang sangat bermakna (p<0,0001) antara jenis kelamin, dislipidemia, dan merokok dengan kejadian PJK pada penderita DM tipe 2 dan terdapat hubungan yang bermakna (p<0,05) antara lama menderita DM, hipertensi, obesitas dengan kejadian PJK pada penderita DM tipe 2.Kata kunci: DM tipe 2, PJK, faktor risikoAbstractThe main causes of mortality and morbidity in type 2 diabetes mellitus (DM) patients is coronary heart disease (CHD) which adults who suffer from DM are two to four times have the risk of heart disease than people without DM. The mechanism of CHD in DM is associated with the presence of atherosclerosis that influenced by various factors. This research has aims to determine the relationship of risk factors for CHD incident in patients with DM. The study was conducted in the Dr. M. Djamil Padang and Cardiac Hospital of West Sumatra from March to August 2013. This research is an analytic study with comparative cross-sectional design. There are 176 DM patient samples that consist of 88 CHD patients and 88 patients without CHD. The data processing used chi-square test by computerized system. The result showed that risk factors that were related with CHD incident in DM patients are gender (p=0,000), long-suffering diabetes (p=0,043), hypertension (p=0,007), dyslipidemia (p=0,000), obesity (p=0,023), and smoking habit (p=0,000). Conclusion: There are marked significant (p<0,0001) relationship between gender, dyslipidemia, and smoking habit with CHD incident in DM patients and significant relationship (p<0,05) between long-suffering diabetes, hypertension, and obesity with CHD incident in DM patients.Keywords: type 2 diabetes mellitus, CHD, risk factor

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Prevalence of type 2 diabetes mellitus among adults aged between 30 to 60 years residing in rural area: a cross sectional study

International Journal of Community Medicine and Public Health ◽

10.18203/2394-6040.ijcmph20183112 ◽

2018 ◽

Vol 5 (8) ◽

pp. 3639

Author(s):

Poornima M. P. ◽

Padmaja R. Walvekar

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Health Hazard ◽

Cross Sectional Study ◽

Sectional Study ◽

Chi Square ◽

Cross Sectional ◽

Important Health

Background: The burden of diabetes mellitus is expected to increase by 58%, from 51 million people in 2010 to 87 million in 2030. In rural India the prevalence rate has increased from 1% to 4-10% over last 20 years. Objective of the study is to know the prevalence of type 2 diabetes mellitus among adults aged between 30 to 60 years residing in rural area.Methods: This community based cross-sectional study was carried out Agasga, the rural field practice area of Dept. of Community Medicine, among 855 adults aged between 30-60 years by using a predesigned & pretested schedule. Statistical analysis was done using percentages and Chi square test.Results: In this study, 67.24% participants were in age group 30-49 years, 32.74% between 50 to 60 years. 48.53% were male and 51.54% were female participants. 79% of the participants were heavy workers, 17.5% were moderate workers and rest 3.5% were sedentary type of workers. 10.9% were illiterate, and rest 91.1% was literates. 69.3% are BPL card holders. The prevalence of diabetes was 9.5%; 10.8% were at risk of getting Diabetes Mellitus. This gender wise difference in diabetes mellitus was not found to be statistically significant (p=0.986).Conclusions: With high degree of heritability, life style factor diabetes could become a major health hazard in India and this underscores the fact that prevention of diabetes must be one of the important health targets for the nation in this century. Early identification of risks will help in prevention and burden of disease.

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