scholarly journals Escherichia coli in Fruit Juice Dispensers

Author(s):  
Ana Claudia Chesca ◽  
Ariana da Silva Costa ◽  
Carolina Bragine Mangucci

Aims: The aim of this study was to evaluate the microbiological quality of juices kept in juice dispensers and sold in retail in canteens and snack bars. Study Design: The study was done with thirty samples of juice, collected in the morning and in the afternoon, obtained at commercial establishments near Uberaba University, whereas two samples were of cashew juice, two samples of tamarind juice, two samples of passion fruit with mango juice, four samples of guava with acerola juice, eight samples of grape juice and twelve samples of passion fruit juice. Place and Duration of Study: Food Microbiology Laboratory - University of Uberaba. Methodology: The temperature at the time of collection was measured. A microbiological analysis was performed. The samples that were positive for thermotolerant coliforms, the analysis was continued, reaching the isolation of Escherichia coli and its subsequent serological identification. Results: The results point to a contamination, over current legal standards, of 66.7% for the samples collected in the morning and of 73.34% for the samples collected in the afternoon. Conclusion: Greater care in good practices is suggested, especially regarding the personal hygiene of handlers, water quality, sanitation and correct temperature control of fruit juice dispensers.

Author(s):  
Anita Kukułowicz

The aim of the research was to compare the microbiological quality of dairy products derived from organic and conventional farming. The test material consisted of milk and milk products (natural yoghurt, cream, curd cheese, rennet ripening cheese, milk). Investigated products were subjected to microbiological analysis and marked for the number of Enterococcus sp., Staphylococcus aureus, yeast and moulds, Escherichia coli as well as to state the presence of Salmonella sp. in them. All samples were free of Salmonella sp. Escherichia coli occurred in 2 samples of curd cheese from organic production. Products derived from organic and conventional production didn’t differ in the average number of microorganisms depending on the type of dairy product, with the exception of ripening rennet cheese, which showed a significant difference in the amount of Staphylococcus aureus.


2013 ◽  
Vol 2 (2) ◽  
Author(s):  
Andressa Tonet ◽  
Alessandra Braga Ribeiro ◽  
Alessandra Mara Bagatin ◽  
Alessandra Quenehenn ◽  
Claudia Cristina Leite Fiori Suzuki

<p>The water from aquaculture is often enriched with nutrients, which provides potential for use in hydroponic production of vegetables such as lettuce. However, this vegetable can be a vehicle for transmitting diseases pathogenic to humans when contaminated. Currently there is a concern about the quality and the risks that food can bring, due to the high incidence of diseases transmitted by these products. In this sense, the objective of this work was to evaluate the sanitary conditions of water and vegetable cultivation in hydroponic and aquaponic systems. Were analyzed 10 samples of water from hydroponic cultivation and 10 samples from aquaponic cultivation, as well as 10 samples of each lettuce growing system, hydroponic, aquaponic and soil. The analysis were performed in duplicate for coliforms at 35&ordm;C and 45&deg;C, coagulase-positive staphylococci and <em>Salmonella </em>spp<em>.. </em>The results showed that both samples of the hydroponic and aquaponic systems presented 100% of coliforms contamination at 35&ordm;C, and that 40% of samples from aquaponic and hydroponic systems presented 30% of <em>Escherichia coli</em>. In the evaluation of lettuce samples it was not detected the presence of <em>Salmonella</em> spp. and staphylococci positive coagulase. However all lettuce samples from aquaponic, hydroponic and soil cultivations showed coliforms at 35&ordm;C (&gt;3NMP/g) and only two samples from the hydroponic system showed coliform at 45&deg;C. It was found that all the cropping systems evaluated in this study provided similar microbiological quality for the final product. So, one can conclude that the samples are suitable for human consumption.</p><p>&nbsp;</p><p>DOI: http://dx.doi.org/10.14685/rebrapa.v2i2.46</p>


2021 ◽  
Vol 10 (20) ◽  
pp. 87-101
Author(s):  
Alysson Rodrigo Fonseca ◽  
Roger Alexandre Nogueira Gontijo

Resumo Este trabalho teve como objetivo identificar e avaliar a qualidade ambiental e microbiológica de nascentes presentes na área urbana de Santo Antônio do Monte -Minas Gerais. Os impactos ambientais nas nascentes foram avaliados a partir da interpretação do Índice de Impacto Ambiental em Nascentes – IIAN. A análise microbiológica da água foi realizada através do Teste do Substrato Cromogênico (Colitest®), que mostra a presença ou ausência de coliformes totais e termotolerantes. Foram identificadas 14 nascentes, porém em quatro destas não foi possível a aplicação dos testes (nascentes 11, 12, 13 e 14), pois encontravam-se secas, soterradas ou tomadas pelo despejo de esgoto. Das nascentes avaliadas, nenhuma atingiu classificação "Ótima" (Classe A) ou "Boa" (Classe B). Apenas uma foi classificada como "Razoável" (Nascente 1; Classe C), cinco como "Ruim" (Nascentes 2, 3, 6, 7 e 8, Classe D) e quatro como "Péssima" (Nascentes 4, 5, 9 e 10, Classe E). Todas apresentaram resultados positivos para presença de coliformes totais e termotolerantes (Escherichia coli). Palavras-chave: Mananciais. Hidrologia. Poluição. Degradação. Abstract This work aimed to identify and evaluate the environmental and microbiological quality of springs present in the urban area of Santo Antônio do Monte -Minas Gerais. The environmental impacts on the springs were assessed based on the interpretation of the Environmental Impact Index on Springs - IIAN. The microbiological analysis of the water was performed using the Chromogenic Substrate Test (Colitest®), which shows either the presence or absence of total and thermotolerant coliforms. Fourteen springs were identified, but in four of them, the application of the test was not possible (springs 11, 12, 13 and 14), as they were dry, buried or taken over by sewage discharge. Out of the springs evaluated, none reached a classification of "Excellent" (Class A) or "Good" (Class B). Only one was classified as "Reasonable" (Source 1; Class C), five as "Bad" (Source 2, 3, 6, 7 and 8, Class D) and four as "Terrible" (Source 4, 5, 9 and 10, Class E). All the springs showed positive results for the presence of total and thermotolerant coliforms (Escherichia coli). Keywords: Springs. Hydrology. Pollution. Degradation. Resumen Este trabajo tuvo como objetivo identificar y evaluar la calidad ambiental y microbiológica de los manantiales presentes en el área urbana de Santo Antônio do Monte - Minas Gerais. Los impactos ambientales en los manantiales se evaluaron sobre la base de la interpretación del Índice de Impacto Ambiental en Manantiales – IIAM. El análisis microbiológico del agua se realizó por medio de la Prueba del Sustrato Cromogénico (Colitest®), que muestra la presencia o ausencia de coliformes totales y termotolerantes. Se identificaron 14 manantiales, pero en cuatro de ellos no fue posible aplicar las pruebas (Manantiales 11, 12, 13 y 14), ya que estaban secos, enterrados o absorbidos por la descarga de aguas residuales. De los manantiales evaluados, ninguno alcanzó clasificación de "Excelente" (Clase A) o "Bueno" (Clase B). Solo uno fue clasificado como "Aceptable" (Manantial 1; Clase C), cinco como "Malo" (Manantiales 2, 3, 6, 7 y 8, Clase D) y cuatro como "Muy malo" (Manantiales 4, 5, 9 y 10, clase E). Todos mostraron resultados positivos para la presencia de coliformes totales y termotolerantes (Escherichia coli). Palabras-clave: Mananciales. Hidrología Contaminación. Degradación.


2020 ◽  
Vol 83 (11) ◽  
pp. 1989-1997 ◽  
Author(s):  
J. McLAUCHLIN ◽  
H. AIRD ◽  
A. CHARLETT ◽  
N. ELVISS ◽  
F. JØRGENSEN ◽  
...  

ABSTRACT Results from monitoring of the microbiological quality of 2,721 samples of ready-to-eat cooked chicken collected between 2013 to 2017 in England were reviewed: 70% of samples were from retail, catering, or manufacture and 30% were imported and collected at English ports. Samples were tested for a range of bacterial pathogens and indicator organisms. Six samples (&lt;1%) had unsatisfactory levels of pathogens that were potentially injurious to health. Neither Salmonella nor Campylobacter were recovered from any samples. Two samples from catering settings contained either an unsatisfactory level of Bacillus cereus (5 × 106 CFU/g) or an unsatisfactory level of coagulase-positive staphylococci (1.6 × 104 CFU/g). Listeria monocytogenes was recovered from 36 samples (1 at manufacture, 26 at catering, and 9 at retail) and in 4 samples, unsatisfactory levels (≥102 CFU/g) were detected (3 samples collected at catering and 1 sample at retail). For L. monocytogenes, there were no significant differences between the rates of contamination for the samples collected from ports, manufacture, retail supermarkets, and other retailers (P = 0.288). There were no differences between the rates of contamination for other potential pathogens detected between samples from different settings. The prevalence of hygiene indicators (Escherichia coli, Enterobacteriaceae, and aerobic colony counts) at import was significantly lower than in samples collected from manufacturers, retail, or catering (P &lt; 0.01). Samples collected from catering gave poorer results than those from all other settings. Regardless of the stage in the food chain, samples from Thailand and from other non–European Union countries were of significantly better microbiological quality with respect to indicator organisms than those from the United Kingdom or from other European Union countries (P = &lt;0.001). HIGHLIGHTS


2013 ◽  
Vol 48 (3) ◽  
pp. 185-192 ◽  
Author(s):  
SA Batool ◽  
SS Tahir ◽  
N Rauf ◽  
R Kalsoom

Freshly prepared and pasteurized fruit juices sold by vendors in local market of Rawalpindi city from 10 locations were analyzed for the microbiological quality. Total viable count (TVC), total coliform, faecal coliform, molds and the presence of pathogenic microorganisms such as E. coli, Pseudomonas, Staphylococcus aureus, Salmonella, and fungi like Aspergillus, Pencillum, Rhizopus were determined .In open fruit juices available in city were highly contaminated with bacteria and fungi. E. coli, Salmonella, Staphylococcus, and Pseudomonas were isolated with different frequency. Aspergillus, Pencillum and Rhizopus were also found in juices especially Aspergillus was with high percentages. The pasteurized juices have less contamination as compared to the fresh juices samples. The number and type of microorganisms recovered from the freshly squeezed fruit juices made them unsafe for drinking. The results of this study demonstrate the unhygienic quality of popular types of market vended freshly squeezed fruit juices and their risk to the consumers. DOI: http://dx.doi.org/10.3329/bjsir.v48i3.17329 Bangladesh J. Sci. Ind. Res. 48(3), 185-192, 2013


2021 ◽  
Vol 7 (1) ◽  
pp. 159-166
Author(s):  
Ajeng Dwi Rahmawati ◽  
Akas Yekti Pulih Asih ◽  
Edza Aria Wikurendra

Background: Based on the preliminary test using laboratory tests from three refill drinking water samples in Jemur Wonosari Village, all three were positive for Coliform. Therefore, researchers are interested in researching Analysis of the Microbiological Quality of Refilled Drinking Water at the Refill Drinking Water Depot in Jemur Wonosari Village. Objectives: Knowing the microbiological quality of refill drinking water produced at the refill drinking water depot in Jemur Wonosari Village. Research Methods: This research is a descriptive study with a cross-sectional quantitative approach using primary and secondary data sources. The population in this study were all 13 refill drinking water depots. The research takes the total population. Refillable drinking water samples were tested at the Gayung Sari Regional Health Service Laboratory. Data analysis used descriptive analysis by comparing the results of laboratory tests with Permenkes 492 of 2010. Results: The results showed that 13 samples found that 13 samples were positive for Coliform, and 13 samples were negative for Escherichia coli. According to Permenkes No. 429 of 2010, the maximum limit per 100 ml of sample is 0. So the 13 samples of refill drinking water based on microbiological parameters, namely Escherichia coli in refilled drinking water, meet the standard while the Coliform does not meet the standard. Conclusion: All refill drinking water depots in Keluraham Jemur Wonosari produce refilled drinking water whose quality does not meet the government's microbiological standards. There is a need for increased supervision and guidance at refill drinking water depots.   Keywords: Drinking Water, Microbiological Analysis, Refill Drinking Water Depot


2004 ◽  
Vol 5 (2) ◽  
Author(s):  
Eliane Rose Serpe ELPO ◽  
Raquel Rejane Bonato NEGRELLE ◽  
Eliane Carneiro GOMES

Analisou-se 10 amostras de gengibre “in natura” com objetivo de caracterizar o perfil microbiológico deste produto comercializado na região metropolitana de Curitiba, PR (Brasil). Para tanto, foram realizadas a determinação do número mais provável (NMP) de coliformes totais, coliformes a 45º C/g e Escherichia coli, e a presença de Salmonella sp em 25 gramas. Os resultados indicaram que 100% das amostras atenderam ao padrão da legislação vigente para Salmonella sp, enquanto que 30% das amostras de gengibre enquadradas como raízes, tubérculos e similares, e cerca de 50% das amostras como especiarias, temperos, condimentos e similares apresentaram coliformes a 45º C/g. Destas amostras analisadas, 20% confirmaram a presença de Escherichia coli. Observou-se que todas as amostras mostraram elevados índices de coliformes totais, apesar de não haver limite máximo estabelecido pela legislação vigente. Os resultados obtidos podem ser indicativos de inadequações nos procedimentos de beneficiamento pós-colheita e de comercialização. Abstract Ten samples of natural ginger, commercialized in Curitiba, Paraná (Brazil), were analyzed for microbiological quality. The microbiological analysis measured the total coliforms, 45° C/g coliforms, the most probable number (MPN) of Escherichia coli and the presence of Salmonella sp. The results revealed that 100% of the samples complied with the required standard for the absence of Salmonella sp. On the other hand, 30% - 50% had 45º C/g coliforms and 20% of them contained Escherichia coli. It was observed that all the samples had a high value for the total coliforms. This is important since the current legislation does not establish any limits. The results obtained may indicate inadequate procedures for post-harvesting and commercial handling.


2013 ◽  
Vol 26 (4) ◽  
pp. 419-429 ◽  
Author(s):  
Permínio Oliveira Vidal Júnior ◽  
Ryzia de Cassia Vieira Cardoso ◽  
Larissa Santos Assunção

OBJECTIVE: This study aimed to describe the trade and microbiological quality of boiled quail eggs on the waterfront of Salvador , Bahia, Brazil, from the child labor perspective. METHODS: This cross-sectional study administered semi-structured questionnaires to 40 underage vendors and performed the microbiological assessment of 40 quail egg samples as follows: mesophilic aerobic microorganism count, coagulase-positive staphylococcus count, estimation of the most probable number of total and thermotolerant coliforms/Escherichia coli, and testing for Salmonella spp.. The results were compared with the standards provided by the Resolution RDC nº 12/2001, National Sanitary Surveillance Agency. RESULTS: The vendors were mostly female (57.5%) students (95.0%) aged 8 to 17 years. The most common reason for working was supplementation of the family income (57.5%). The mean gross income was R$38.31/day. Most of them presented inadequate personal hygiene but they recognized that foods could cause diseases. Many (47.5%) vendors reported washing their hands up to twice daily. Mean mesophilic aerobic microorganism and coagulase-positive staphylococcus counts were 2.43 and 2.01 log colony-forming unit/g, respectively, and the estimated thermotolerant coliform contamination was 0.98 log most probable number/g. Escherichia coli was found in 15.0% of the samples and none contained Salmonella spp. Most (55.0%) samples were noncompliant with the legislation. CONCLUSION: The results evidenced the presence of minors selling quail eggs on beaches of Salvador and suggest risk to consumers' health because of the detected contamination and vendors' ignorance of principles of hygiene.


UNICIÊNCIAS ◽  
2019 ◽  
Vol 23 (1) ◽  
pp. 38
Author(s):  
Madalena Miguelina Zablocki ◽  
Crislaine Aparecida Paludo ◽  
Márcia Keller Alves ◽  
Simara Rufatto Conde

O uso da multimistura é considerado como uma excelente alternativa para suplementação alimentar em programas de intervenção nutricional para populações carentes no Brasil. No entanto, problemas relacionados à qualidade microbiológica têm aumentado a preocupação no que diz respeito à segurança do produto. Este estudo experimental teve como objetivo determinar a composição química e microbiológica da multimistura. Para tal, foram adquiridas amostras de multimistura produzidas e comercializadas no interior do Estado do Rio Grande do Sul, Brasil. As análises químicas foram realizadas na Unidade Integrada Vale do Taquari de Ensino Superior (UNIVATES) de Lajeado/RS, onde as quantidades de proteínas, de carboidratos, de lipídios, de cálcio (Ca), de fósforo (P), de cobre (Cu), de ferro (Fe), de magnésio (Mg), de potássio (K) e de zinco (Zn) foram determinadas. As medições obtidas foram comparadas com os valores de referência da Ingestão Dietética de Referência (IDRs) para crianças de 4 a 8 anos. As análises microbiológicas foram realizadas pelo Laboratório de Microbiologia, na UNIVATES. Os valores encontrados de macronutrientes e micronutrientes foram: 56,51% de carboidratos; 17,88% de proteínas; 15,56% de lipídios; 0,1% de Ca; 0,11% de P; 1,3mg de Cu; ausência de Fe; 0,11% de Mg; 0,96% de P; 9,1mg de Zn. Os valores de macronutrientes e micronutrientes não obtiveram altos percentuais de representação, ficando quase a totalidade dos nutrientes abaixo do recomendado pela IDRs. Os resultados da análise microbiológica mostraram presença de coliformes termotolerantes, de coliformes totais, Staphylococcus aureus, Escherichia coli e ausência de Salmonella sp. Em conjunto, os dados deste trabalho mostram que o produto analisado é um alimento de baixo valor nutricional e de segurança alimentar duvidosa, o qual pode colocar em risco a saúde de crianças ou indivíduos de qualquer idade, que possam vir a consumi-lo. Palavras-chave: Análise de Alimentos. Contaminação. Disponibilidade Nutricional. AbstractThe use of multimixture is considered an excellent alternative for food supplementation in nutritional intervention programs for needy populations in Brazil. However, problems related to microbiological quality have increased concern regarding product safety. This experimental study had as goal to determine the chemical and microbiological composition of  multimixture. For this purpose, samples of multimixture produced and marketed in the countryside of the state of Rio Grande do Sul (RS), Brazil, were acquired. The chemical analyzes were carried out at  Vale do Taquari Integrated Unit of Higher Education (UNIVATES) in Lajeado/RS, where the amounts of proteins, carbohydrates, lipids, calcium (Ca), phosphorus (P), cuprum (Cu), ferrum (Fe), magnesium (Mg), kalium (K) and zinc (Zn) were determined. The results of measurements were compared with the Dietary Reference Intakes (DRIs) reference values for children aged 4 to 8 years. Microbiological analyzes were performed by the Laboratory of Microbiology, at UNIVATES. The values of macronutrients and micronutrients were: 56.51% of carbohydrates; 17.88% of proteins; 15.56% of lipids; 0.1% of Ca; 0.11% of P; 1.3 mg of Cu; absence of Fe; 0.11% of Mg; 0.96% of P; and 9.1 mg of Zn. Macronutrient and micronutrient values did not reach high percentages of representation, with almost all of the nutrients being below recommended by the DRIs. In addition, the results of the microbiological analysis showed presence of thermotolerant coliforms, total coliforms, Staphylococcus aureus, Escherichia coli and absence of Salmonella sp. Taken together, data from this work show the product under review is a food of low nutritional value and of dubious food safety, which may endanger the  children’s or individuals’ health of any age who might be able to consume it. Keywords: Food Analysis. Contamination. Nutritive Value.


1995 ◽  
Vol 31 (5-6) ◽  
pp. 75-79 ◽  
Author(s):  
M. Würzer ◽  
A. Wiedenmann ◽  
K. Botzenhart

In Germany the application of procedures such as flocculation and filtration in the preparation of drinking water results in the annual production of an estimated 500,000 t of sediments and sludges. Some of these residues have a potential for being reused, for example in agriculture, forestry, brickworks or waste water treatment. To assess the microbiological quality of residues from waterworks methods for the detection of enterobacteria, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Legionella, poliovirus, Ascaris suis eggs and Cryptosporidium have been evaluated regarding their detection limits and were applied to various residues from German waterworks. Results show that sediments and sludges may contain pathogenic bacteria, viruses and protista. When residues from waterworks are intended to be reused in agriculture or forestry the microbiological quality should therefore be considered.


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