scholarly journals Phytoremediation of Heavy Metals Using Some Selected Leguminous Crops

Author(s):  
G. I. Ameh ◽  
H. O. Nwamba ◽  
V. S. Njom ◽  
E. C. Ofordile

The phytoremediation of heavy metals (cadmium, chromium, copper and lead) using Phaseolus vulgaris and Arachis hypogaea were investigated using standard techniques. Heavy metal polluted soil samples were collected from Crush Rock Industries Ishiagu, Ebonyi State and heavy metal free soil samples (used as control) were obtained from Ebonyi State Ministry of Agriculture, Ishiagu Station. The seeds of the two plants were collected from the Enugu State Ministry of Agriculture. The experimental setup consists of 4 contaminated potted soils each of P. vulgaris and A. hypogaea. Another 4 potted soils not contaminated with heavy metals served as control. Soil analysis was carried out prior to planting. The polluted soil sample had slightly acidic pH (pH was 6.34±0.29), higher Cation Exchange Capacity (21.80±0.33), higher Cd (25.18±0.34), Cr (10.20±0.21), Cu (28.54±0.49) and Pb (9.92±0.36) levels but lesser soil organic carbon (0.87±0.10). After the duration of 62 days the plants were harvested, their leaves and roots were digested and subjected to further experimental tests [determination of Metal concentration, transfer factor and bioaccumulation factor (BAF)]. A. hypogaea showed highest Cd translocation factor 1.63±0.08, TF<1 was observed in all the plants examined for Cr. A. hypogaea showed the highest BAF for Cd, (1.16±0.08). BAF<1 was observed for Cr and Cu in the two plants examined. The BAF of A. hypogaea was higher than that of P. vulgaris. Plants from the control showed zero to very minute concentration of heavy metals in their tissues. Although the study plants are food crops, they can also play a role in the phytoremediation of some heavy metals.

Plant Omics ◽  
2019 ◽  
pp. 87-92
Author(s):  
Ghalia Saleem Aljeddani

Environmental pollution is causing damage to the flora and fauna, resulting in a number of negative impacts on the environment. The present study aims towards evaluation of soil heavy metals (chromium, lead, and nickel) from different locations of Jeddah using leaves of two plant species. Two naturally grown plant species, Prosopis juli flora and Conocarpus lancifolius were collected from four different sites. The soil samples were collected near the factories, main roads, and branch roads in Jeddah. The soil samples were dried and sieved through a 2mm plastic sieve to remove large gravel-sized materials. Soil texture, pH, and EC evaluation were conducted. The amount of Cr, Pb, and Ni was determined using Atomic Absorption Spectrophotometer Model Inductivity coupled plasma emission spectrometer ICP (PARKIN ELEMER). SPSS, ANOVA, and Post-Hoc Test were used to analyze the data. The results showed that the amount of heavy metal in the soil of Prosopis juli flora were between 0.74-54.0μg/g D.wt. for Cr, 0.31-4.64 μg/g D.wt. for Pb and 1.22-24.5 μg/g D.wt. for Ni. The range of Cr, Pb and Ni in the leaves were between 0.04-13.3.0μg/g D.wt. for Cr, 0.06-20.0 μg/g D.wt. for Pb and 0.16-9.0μg/g D.wt. for Ni. For the soil of Prosopis juli flora, the EC ranged between 0.27- 1.05 mS/cm; whereas, pH ranged from 7.29-7.55 and 78.5-96.5% for sand, 1.0-16.5% for silt, and 1.0-6.0 for clay. There was a significant correlation between the soil textures, clay, and silt at selected sites. Phytoremediation is an effective strategy to overcome the effects of heavy metals including Cr, Pb, and Ni that are being absorbed in plants.


2018 ◽  
Vol 14 (2) ◽  
pp. 72-84
Author(s):  
C O AKACHUKU ◽  
V P TOMBERE

The study examined the effect of polluted soil on early growth performance of Pentaclethra macro-phlylla. The objectives of the study were to determine the heavy metal content of the soil and the effect on the growth of this species. Soil samples were collected from an abandoned Sunshine battery facto-ry in Essien Udim Local Government Area of Akwa Ibom State using soil auger. The samples, collect-ed at different soil depths and at various distances, were divided into two parts. A part was analysed in the laboratory to determine the heavy metal content of the soil using digestion and atomic absorption methods, while the other part was used in raising Pentaclethra macrophyla seedlings in polypots. The result of analysis of variance of the soil samples considering the distances showed significant differ-ence in the distribution of copper and lead only (P<0.05), while no significant difference occurred among the concentrations of all the heavy metals at different soil depths. The ranges of concentration of metals in the polluted soil samples in mg/kg were Zn (13.90-41.30), Bo (11.40-27.10), Cd (61.10-77.10), Cu (5.35-49.75), Pb (53.10-153.70), As (0.40-4.50) and Se (1.80-4.30), while the control rec-orded the least amount of Pb (2.40-4.30 mg/kg). On the average, seedlings raised in soils collected from the point of waste disposal (epicentre) had the least growth values in terms of total leaf number (166), stem height (21.90cm), branch number (1.4) and branchlet number (8.8). This signifies stunted growth, which is likely due to the high concentrations of Pb and Cu. Factories should therefore be compelled to eliminate heavy metals before disposing their waste into the environment.


Author(s):  
G. I. Ameh ◽  
H. O. Nwamba ◽  
C. D. Nwani ◽  
E. C. Ofordile

Aims: To investigate the effect of Heavy metals on the growth of Zea mays (Z. mays) and Sorghum bicolour (S. bicolour). Study Design: Laboratory-experimental design was used in this study. Place of Study: The heavy metal polluted soil samples were collected from Crush Rock Industries Ishiagu, Ebonyi State, Nigeria, while control soil samples were obtained from the Zoological Garden of the Department of Applied Biology and Biotechnology, Enugu State University of Science and Technology. Methodology: The seeds of the two plants were collected from the Enugu State Ministry of Agriculture. The experimental setup consisted of 16 contaminated potted soils, 8 each for Z. mays and S. bicolor. Another 8 potted soils not contaminated with heavy metals served as control. Fourteen days after germination, the following growth parameters were measured (in cm); plant heights, number of leaves, stem girth, and leaf area. Results: The polluted soil sample was slightly acidic; (pH was 6.34±0.29). It had a higher cation exchange capacity (21.80±0.33), Cd (25.18±0.34), Cr (10.20±0.21), Cu (28.54±0.49), Pb (9.92±0.36), but lesser soil organic carbon (0.87±0.10). Plant samples cultivated in contaminated soil showed the least favourable vegetative growth. Conclusion: Comparing the results obtained from the control sample, it was deduced that these heavy metals have adverse effect on the vegetative growth of Z. mays and S. bicolor.


Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1057
Author(s):  
Ehab Azab ◽  
Ahmad K. Hegazy

Heavy metal-contaminated soil constitutes many environmental concerns. The toxic nature of heavy metals poses serious threats to human health and the ecosystem. Decontamination of the polluted soil by phytoremediation is of fundamental importance. Vegetation is an appealing and cost-effective green technology for the large-scale phytoremediation of polluted soils. In this paper, a greenhouse experiment was carried out to test the potential of Rhazya stricta as a heavy metal phytoremediator in polluted soil. Plants were grown for three months in pots filled with soils treated with the heavy metals Cd, Pb, Cu, and Zn at rates of 10, 50, and 100 mg/kg. The bioaccumulation factor (BCF) and translocation factor (TF) were calculated to detect the ability of R. stricta to accumulate and transfer heavy metals from soil to plant organs. The results showed that under increasing levels of soil pollution, the bioconcentration of Cd and Zn heavy metals showed the highest values in plant roots followed by leaves, whereas in the case of Pb and Cu, roots showed the highest values followed by stems. Heavy metals accumulation was higher in roots than in stems and leaves. The BCF of Zn reached the highest values in roots and stems for 10 mg/kg soil treatment, followed by the BCFs of Cd, Cu, and Pb. The TF for the different heavy metal pollutants’ concentrations was less than unity, suggesting that the plants remediate pollutants by phytostabilization. The TF values ranged from higher to lower were in the order Zn > Cu > Cd > Pb. The rapid growth of R. stricta and its tolerance of heavy metals, as well as its ability to absorb and accumulate metals within the plant, recommends its use in the phytoremediation of slightly polluted soils in arid lands by limiting the heavy metals transport.


2018 ◽  
Vol 69 (7) ◽  
pp. 1695-1698
Author(s):  
Marin Rusanescu ◽  
Carmen Otilia Rusanescu ◽  
Gheorghe Voicu ◽  
Mihaela Begea

A calcium bentonite from Orasu Nou deposit (Satu Mare Romania) was used as raw material. We have conducted laboratory experiments to determine the influence of bentonite on the degree of heavy metal retention. It has been observed that the rate of retention increases as the heavy metal concentration decreases. Experimental studies have been carried out on metal retention ( Zn) in bentonite. In this paper, we realized laboratory experiments for determining the influence of metal (Zn) on the growth and development of two types of plants (Pelargonium domesticum and Kalanchoe) and the effect of bentonite on the absorption of pollutants. These flowers were planted in unpolluted soil, in heavy metal polluted soil and in heavy metal polluted soil to which bentonite was added to observe the positive effect of bentonite. It has been noticed that the flowers planted in unpolluted soil and polluted with heavy metals to which bentonite has been added, the flowers have flourished, the leaves are still green and the plants whose soils have been polluted with heavy metals began to dry after 6 days, three weeks have yellowish leaves and flowers have dried. Experiments have demonstrated the essential role of bentonite for the removal of heavy metals polluted soil.


2021 ◽  
Vol 11 (15) ◽  
pp. 7099
Author(s):  
Inkyeong Moon ◽  
Honghyun Kim ◽  
Sangjo Jeong ◽  
Hyungjin Choi ◽  
Jungtae Park ◽  
...  

In this study, the geochemical properties of heavy metal-contaminated soils from a Korean military shooting range were analyzed. The chemical behavior of heavy metals was determined by analyzing the soil pH, heavy metal concentration, mineral composition, and Pb isotopes. In total, 24 soil samples were collected from a Korean military shooting range. The soil samples consist of quartz, albite, microcline, muscovite/illite, kaolinite, chlorite, and calcite. Lead minerals, such as hydrocerussite and anglesite, which are indicative of a transformation into secondary mineral phases, were not observed. All soils were strongly contaminated with Pb with minor concentrations of Cu, Ni, Cd, and Zn. Arsenic was rarely detected. The obtained results are indicated that the soils from the shooting range are contaminated with heavy metals and have evidences of different degree of anthropogenic Pb sources. This study is crucial for the evaluation of heavy metal-contaminated soils in shooting ranges and their environmental effect as well as for the establishment of management strategies for the mitigation of environmental risks.


2002 ◽  
Vol 11 (4) ◽  
pp. 285-300 ◽  
Author(s):  
V. MÄNTYLAHTI ◽  
P. LAAKSO

Increasing concentrations of arsenic and heavy metals in agricultural soils are becoming a growing problem in industrialized countries. These harmful elements represent the basis of a range of problems in the food chain, and are a potential hazard for animal and human health. It is therefore important to gauge their absolute and relative concentrations in soils that are used for crop production. In this study the arsenic and heavy metal concentrations in 274 mineral soil samples and 38 organogenic soil samples taken from South Savo province in 2000 were determined using the aqua regia extraction technique. The soil samples were collected from 23 farms.The elements analyzed were arsenic, cadmium, chromium, copper, mercury, nickel, lead and zinc. The median concentrations in the mineral soils were:As 2.90 mg kg –1, Cd 0.084 mg kg –1, Cr 17.0 mg kg –1, Cu 13.0 mg kg –1, Hg 0.060 mg kg –1, Ni 5.4 mg kg –1, Pb 7.7 mg kg –1, Zn 36.5 mg kg –1. The corresponding values in the organogenic soils were:As 2.80 mg kg –1, Cd 0.265 mg kg –1, Cr 15.0 mg kg –1, Cu 29.0 mg kg –1, Hg 0.200 mg kg –1, Ni 5.9 mg kg –1, Pb 11.0 mg kg –1, Zn 25.5 mg kg –1. The results indicated that cadmium and mercury concentrations in the mineral and organogenic soils differed. Some of the arsenic, cadmium and mercury concentrations exceeded the normative values but did not exceed limit values. Most of the agricultural fields in South Savo province contained only small amounts of arsenic and heavy metals and could be classified as “Clean Soil”. A draft for the target values of arsenic and heavy metal concentrations in “Clean Soil” is presented.;


Forests ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1608
Author(s):  
Aslihan Esringü ◽  
Metin Turan ◽  
Asli Cangönül

Heavy metal pollution is among the important environmental problems in the world. Many techniques have already been used to remove the heavy metals such as lead (Pb) and cadmium (Cd). Among them, the phytoremediation method is an environmentally friendly and green technology. This study was carried out to determine the efficiency of fulvic acid (FA) application in removing Pb and Cd from polluted soil using Tagetes eracta L. and Zinnia elegans Jacq. ornamental plants. The results indicated that, FA application, number of flower per plants, and plant fresh weight of Tagetes eracta plants and Zinnia elegans plants increased 187.5%, 104.5% and 155.5%, 57.7%, respectively with application of 7000 mg L−1 FA at 100 mg kg−1 Pb pollution condition, whereas 42.85%, 16.5%, and 44.4–36.1% with application of 7000 mg L−1 FA at 30 mg kg±1 Cd pollution condition, respectively. With the FA application in the Zinnia elegans plant, the root part has accumulated 51.53% more Pb than the shoot part. For Cd, the shoot part accumulated 35.33% more Cd than the root. The effect of FA application on superoxide dismutase (SOD), peroxidase (POD) and, catalase (CAT) of the Tagetes eracta were decreased as 32.7%, 33.1%, and 35.1% for Pb, 21.2%, 25.1%, and 26,1%, for Cd, and 15.1%, 22.7%, and 37.7% for Pb, and 7.55%, 18.0%, and 18.8% for Cd were in Zinnia elegans respectively. In conclusion, Tagetes eracta and Zinnia elegans can not be recommended for remediation of Pb and Cd polluted area, but FA can be recommended for Pb and Cd stabilization in polluted soil.


2020 ◽  
Vol 10 (27) ◽  
pp. 200911
Author(s):  
Aung Zaw Tun ◽  
Pokkate Wongsasuluk ◽  
Wattasit Siriwong

Background. Artisanal and small-scale mining activities are widely practiced globally. Concentrations of heavy metals associated with gold, such as copper (Cu), zinc (Zn), arsenic (As), cadmium (Cd), mercury (Hg) and lead (Pb) can increase in the environment as a result of mining activities, leading to environmental pollution and pose toxicity risks to humans and animals. Objectives. The aim of the present study was to investigate soil concentrations of toxic heavy metals in placer small-scale gold mining operations in Myanmar. Methods. Soil samples were collected from three placer small-scale gold mining sites: Site A located in the Hmawbon public protected forest, Site B and Site C, situated in the Nant-Kyin reserved forest around Nar Nant Htun village. At each site, soil samples were collected from four gold mining stages (ore processing, sluicing, panning, and amalgamation). Atomic absorption spectroscopy was utilized to examine the concentrations of As, Cd, Pb, and Hg. Results. The highest heavy metal concentrations were generally found in the amalgamation stages across all the gold mining sites. Across the three mining sites, the maximum heavy metal concentrations in the amalgamation stage were 22.170 mg.kg−1 for As, 3.070 mg.kg−1 for Cd, 77.440 mg.kg−1 for Hg, and 210.000 mg.kg−1 for Pb. Conclusions. The present study examined the concentrations of As, Cd, Hg and Pb in the soil of several small-scale gold mining sites in Banmauk Township, Myanmar. The results demonstrated the presence of high concentrations of heavy metals in the soil of the gold mining sites. Miners in this area work without proper personal protective equipment, and frequent exposure to heavy metals in the soil may cause adverse health effects. The present study provides baseline data for future risk assessment studies of heavy metal contamination in gold mines. Competing Interests. The authors declare no competing financial interests


Author(s):  
Muibat Fashola

Introduction: Indiscriminate dumping of spent oils enriched with heavy metals has led to increase in heavy metals load in the soil. Heavy metals exert toxic effects on biodegradation of organic pollutant in cocontaminated soil and there is need to find suitable strategies for their removal. Aim: The aim of this study was to assess the heavy metals resistance capability of indigenous Bacillus species in hydrocarbon polluted soil to nickel (Ni), Cadmium (Cd), Lead (Pb) and Chromium (Cr). Materials and Methods: Heavy metal tolerant bacteria were isolated from hydrocarbon polluted soil using Luria-Berthani agar supplemented with the respective metals and spread plate techniques. The isolates were putatively identified on the basis of their colonial morphology and biochemical characteristics and their antibiotics susceptibility pattern were evaluated using disc diffusion method. Results: The maximum tolerable concentration (MTC) of the four heavy metals to the selected isolates was 2 mM. Four bacteria isolates able to withstand the MTC were putatively identified as Bacillus subtilis, Bacillus megaterium, Bacillus laterosporus and Bacillus polymyxa. Out of the four Bacillus species, only B. laterosporus did not show multiple tolerance to the tested antibiotics which show that there is correlation between heavy metal tolerance and antibiotics resistance by the isolates. Conclusion: Multiple heavy metal tolerance Bacillus spp. were isolated from crude oil polluted soil. These bacteria could be suitable agents for bioaugmentation of hydrocarbon polluted soil co-contaminated with heavy metals.


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