Phytochemical Screening and Antibacterial Activity of Citrus sinensis (L.) Osbeck [Orange] and Citrus aurantifolia (Cristm.) Swingle [Lime] Stem from Bacteria Associated with Dental Caries

2018 ◽  
Vol 8 (4) ◽  
pp. 1-9
Author(s):  
M Nata’ala ◽  
M Dalhat ◽  
B Omoye ◽  
A Isah ◽  
S Kabiru ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Dental Caries ◽  
Citrus Sinensis ◽  
Phytochemical Screening ◽  
Citrus Aurantifolia

scholarly journals PERBANDINGAN AKTIVITAS ANTIBAKTERI INFUSA DAUN JERUK NIPIS (CITRUS AURANTIFOLIA) DAN DAUN JERUK PURUT (CITRUS HYSTRIX) TERHADAP BAKTERI ESCHERICHIA COLI

2020 ◽  
Vol 3 (1) ◽  
pp. 39-46
Author(s):  
Saadah Siregar ◽  
Indriani Indriani ◽  
Vincentia Vincentia Ade Rizky ◽  
Visensius Visensius Krisdianilo ◽  
Romauli Anna Teresia Marbun
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Green Light ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Citrus Aurantifolia ◽  
Water Solvent ◽  
Dark Green ◽  
Light Green ◽  
Macroscopic Examination

Plant Lime (Citrus aurantifolia) and Plant Kaffir Lime (Citrus hystrix) including Rutacea family. Part of the plant lemon and lime are used as a drug other than fruit and leaves that can be used as medicine. This study aims to determine the antibacterial activity infuse lime leaves and lime leaves. Examination of the characteristics simplicia macroscopic examination. Phytochemical screening of compounds includes examining alkaloids, flavonoids, tannins and infuse saponin. Ekstrak conducted using distilled water solvent. Test of antibacterial activity against Escherichia coli bacteria carried by the agar diffusion method using a paper disc. Results obtained from macroscopic examination for lime leaves are fresh leaves leaf-shaped single smooth surface and the lower surface of the leaves are light green, the dark green upper surface. If torn, lime leaves produce coarse fibers. The leaves are small with a width of 3-5 cm. For lime leaves are fresh leaves, leaf litter pinnate compound leaves one. Child leaf blade oval to oblong, base rounded or blunt, the blunt end up pointed, 8-15 cm long, 2-6 cm wide, the upper surface colour some what shiny dark green, light green below the surface. Results of phytochemical screening simplicia powder lime leaves and lime leaves that contain a class of alkaloids, flavonoids and tannins. Antibacterial activity test results showed that there were differences between the antibacterial infusion lime leaves and lime leaves kaffir lime leaves which are more effective against the bacteria Escherichia coli compared kaffir lime leaves.

Antibacterial Activity and Phytochemical Screening of Olea Europaea Leaves from Algeria

2012 ◽  
Vol 3 (1) ◽  
pp. 66-69 ◽  
Author(s):  
Nahal Bouderba Nora ◽  
Kadi Hamid ◽  
Moghtet Snouci ◽  
Meddah Boumedien ◽  
Moussaoui Abdellah
Keyword(s):  
Antibacterial Activity ◽  
Olea Europaea ◽  
Phytochemical Screening ◽  
Olea Europaéa

scholarly journals Lactobacillus reuteri BM53-1 Produces a Compound That Inhibits Sticky Glucan Synthesis by Streptococcus mutans

2021 ◽  
Vol 9 (7) ◽  
pp. 1390
Author(s):  
Masafumi Noda ◽  
Naho Sugihara ◽  
Yoshimi Sugimoto ◽  
Ikue Hayashi ◽  
Sachiko Sugimoto ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Dental Caries ◽  
Lactobacillus Reuteri ◽  
Early Stage ◽  
Pcr Analysis ◽  
Cariogenic Bacteria ◽  
Qrt Pcr ◽  
Actinidia Polygama ◽  
Glucan Synthesis

Cariogenic bacteria, such as Streptococcus (S.) mutans and S. sobrinus, produce insoluble and sticky glucans as a biofilm material. The present study demonstrates that a lactic acid bacterium (LAB) named BM53-1 produces a substance that inhibits the sticky glucan synthesis. The BM53-1 strain was isolated from a flower of Actinidia polygama and identified as Lactobacillus reuteri. The substance that inhibits sticky glucan synthesis does not exhibit antibacterial activity against S. mutans. The cariogenic S. mutans produces glucans under the control of three glucosyltransferase (GTF) enzymes, named GtfB, GtfC, and GtfD. Although GtfB and GtfC produce insoluble glucans, GtfD forms soluble glucans. Through quantitative reverse-transcriptional (qRT)-PCR analysis, it was revealed that the BM53-1-derived glucan-production inhibitor (GI) enhances the transcriptions of gtfB and gtfC genes 2- to 7-fold at the early stage of cultivation. However, that of gtfD was not enhanced in the presence of the GI, indicating that the glucan stickiness produced by S. mutans was significantly weaker in the presence of the GI. Our result demonstrates that Lb. reuteri BM53-1 is useful to prevent dental caries.

Green Synthesis of Silver Nanoparticles using Citrus aurantifolia (Bangladeshi Lemon Leaf) Extract and Its Antibacterial Activity

2021 ◽  
Author(s):  
Joy James Costa ◽  
Hassan Hosseinzadeh ◽  
Dabasish Kumar Saha ◽  
Shihab Uddin Al Mahmud ◽  
Bhuiyan Mohammad Mahtab Uddin ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Citrus Aurantifolia

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

scholarly journals Preliminary phytochemical screening and susceptibility of bacteria pathogens to whole extract of evolvulus alsinoides (L.)

1970 ◽  
Vol 18 ◽  
pp. 16-20
Author(s):  
BA Omogbai ◽  
FA Eze
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Volatile Oil ◽  
Salmonella Typhi ◽  
Phytochemical Screening ◽  
Evolvulus Alsinoides ◽  
And Staphylococcus Aureus

Context: Plant based antimicrobial represent a vast untapped source for medicines and further exploration of plant antimicrobial neeto occur. Evolvulus alsinoides (L) (Convolvulaceae) is a perennial herb is used in traditional medicine in East Asia, India, Africa and Philippines to cure fever, cough, cold, venereal diseases, azoospermia, adenitis and dementia.   Objective: The objective of this research was to evaluate the antimicrobial activity of the extracts of E. alsinoides on some clinical microbial isolates.   Materials and Methods: The ed thanolic and aqueous extracts of the whole plant (leaves and twigs) were analysed for alkanoids, tannins, glycosides, steroids, flavonoids, saponins, volatile oil and resins. The determination of antibacterial activity was done using the agar well diffusion technique. Pure cultures of pathogenic bacteria such as Bacillus cereus, Staphylococcus aureus, Micrococcus leutus, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi were used for antibacterial activity assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).   Results: The ethanolic extract of the plant had MIC values ranging from 16 mg/ml to 512.5 mg/ml. The least MIC was 16mg-ml against Salmonella typhi while Bacillus cereus and Staphylococcus aureus showed the highest MIC of 512.5 mg-ml. In the aqueous extract the MIC ranged between 512.5 to >1025 mg/ml. Salmonella typhi, Micrococcus luteus and Staphylococcus aureus were not inhibited by the water extract. Phytochemical result showed ethanol to be a better solvent for the extraction of the bioactive agents in this plant which include: glycosides, alkaloids, saponins, tannins, flavonoids and volatile oil.   Conclusion: In this study the gram-negative organisms had the lowest MICs and MBCs. This suggests their higher susceptibility to the extract of this plant. On the basis of the result obtained in this investigation it can be concluded that ethanol extract of Evolvulus alsinoides had significant in vitro broad spectrum antimicrobial activity.   Keywords: Evolvulus alsinoides; Phytochemical screening; Antibacterial activity. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8769 JBS 2010; 18(0): 16-20

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