scholarly journals Acclimatization of Tissue Culture Pineapple Plantlet Using Semi-Autotrophic Hydroponics Technique in Comparison with Other Conventional Substrates

2021 ◽  
pp. 61-67
Author(s):  
Y. O. Olagunju ◽  
A. O. Aduloju ◽  
P. E. Akin-Idowu ◽  
C. O. Esuola
Keyword(s):  
Tissue Culture ◽  
Survival Rate ◽  
Plant Height ◽  
Root Length ◽  
Block Design ◽  
Research Unit ◽  
Ananas Comosus ◽  
Biotechnology Research ◽  
Effective Media ◽  
Significant Difference

Aims: Previously conventional substrates consisting of topsoil mixtures have produced low yield and low survival rate of the tissue culture plantlets. Semi-Autotrophic Hydroponics (SAH) technique is being compared with Sawdust (SD) and Topsoil (TS) as a suitable method of acclimatization and further rooting of the plantlets. This research is aimed at optimizing the protocol for acclimatization of tissue culture pineapple plantlets. Study Design:  Experimental Research Design. Place and Duration of Study: The experiment was conducted at the Biotechnology Research Unit, Tissue Culture Laboratory, National Horticultural Research Institute, Jericho-Idi-Ishin, Ibadan. Feb 2021 – April 2021. Methodology: The technique employed was a Randomized Complete Block Design (RCBD) with 10 samples per treatment in 6 replicates. 5 treatments were investigated in this research. Data analysis was done with the use of SAS statistical analysis software (p<0.05). Fresh crowns of Ananas comosus (pineapple) were extracted and were cultivated in test tubes containing full MS media. The plantlets were sub-cultured twice, after which they were taken into the hardening chamber. The plantlets were acclimatized in the Semi-Autotrophic Hydroponics substrate and other substrates – Topsoil(TS), Sawdust(SD), Sawdust + Topsoil (3:1), and Sawdust + Topsoil (1:3). Results: The result shows that the mean difference in Plant Height and Root Length for SAH substrate was significant as against other substrates used in this study (p<0.05) with SAH having the highest value of 3.50cm for plant height and 2.53cm for root length.  A 100% survival rate was observed for the plantlets grown SAH media as compared to the ones acclimatized on Topsoil and Sawdust combinations which showed a 50% survival rate. There was no significant difference in the number of leaves among all the groups of substrates. Conclusion: In conclusion, SAH media is a very effective media for the hardening and acclimatization of micro-propagated plantlets.

scholarly journals RESPON PUPUK KANDANG KAMBING DAN PUPUK NPK PADA PERTUMBUHAN DAN HASIL TANAMAN TERUNG HIJAU (Solanum melongena L.).

2016 ◽  
Vol 10 (1) ◽  
pp. 53-62
Author(s):  
Visca R Yuanita ◽  
Tri Kurniastuti ◽  
Palupi Puspitorini
Keyword(s):  
Plant Height ◽  
Block Design ◽  
Solanum Melongena ◽  
Growth And Yield ◽  
Leaf Number ◽  
Fruit Length ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Npk Fertilizer ◽  
Goat Manure

Research about influence of goat manure and NPK fertilizer on the growth and yield of green eggplant (Solanum mengolena L.) has been conducted in field village farmer-owned Semen Gandusari District of Blitar which took place in March-April 2016. The aim of this study was to know the interaction for among goat manure and NPK fertilizer on the growth and yield of green eggplant as well as the effect of each factor. This study uses Randomaized Complete Block Design (RCBD) factorial consisting of 16 treatment and each 3 replicates ie: P0M0, P0M1, P0M2, P0M3, P1M0, P1M1, P1M2, P1M3, P2M0, P2M1, P2M2, P2M3, P3M0, P3M1 , P3M2, P3M3. The data taking were plant height, leaf number, fruit length, fruit diameter and weight of fruit per plant. The data were analyzed to F test as followed by a 5% test Honestly Significant Difference (HSD) error level of 5%. The experimental results very significant effect on plant height, leaf number, fruit length and weight of fruits per plant and the effect is not noticeable to the diameter of fruit plants green eggplant. The combined use goat manure 750 g / plant and NPK fertilizer 60 g / plant (P2M3) is proper fertilization to improve the growth and best yield of green eggplant with the highest total weight of the fruit crop that yields 1576.6 grams

scholarly journals Respon Pertumbuhan Kelapa Sawit (Elaeis Guineensis Jacq.) Varietas Dyxp Dumpy pada Kondisi Stres Air di Pembibitan Awal

2018 ◽  
Vol 3 (1) ◽  
pp. 17
Author(s):  
Wagino Wagino ◽  
Sri Murti Tarigan ◽  
Eka Bobby Febrianto
Keyword(s):  
Plant Height ◽  
Oil Palm ◽  
Root Length ◽  
Stomatal Density ◽  
Block Design ◽  
Dry Weight ◽  
Base Diameter ◽  
Root Volume ◽  
Root Dry Weight ◽  
Wet Weight

<p>Global climate exchange especially rainfallspurs the growth of oil palm adapt to environmental conditions, so the development of oil palm plantations requires seeds that are able to adapt well in drought conditions.This study aims to determine whether water stress affects the growth of oil palm seedlings and sees the response of the level of resistance of oil palm seeds to drought stress.This research was carried out in the greenhouse garden experiment Medan STIPAP was conducted from January to June 2018. This study used Non-factorial Randomized Block Design (RBD), which consisted of 3 levels, 3 samples and 3 replications. Parameters to be observed were plant height, stem base diameter, leaf number, root wet weight, root dry weight, plant wet weight, plant dry weight, root volume, root length, stomatal density and number of stomata.The results showed that the difference in giving A1 water (100%) was significantly different from A2 (60%) and A3 (20%) on the parameters of oil palm seedling growth especially for the parameters of plant height, root wet weight, root dry weight, plant wet weight, plant dry weight, root volume, and root length while the base diameter of the stem, number of leaves, stomatal density and number of stomata give no significant effect. Drought stress effected to reduce the growth of stem diameter.</p>

scholarly journals The Effect of NPK-Zeo Fertilizer on Growth and Production of Cucumber (Cucumis sativus L.) in Iwoimopuro Village, Kolaka District

2019 ◽  
Vol 2 (1) ◽  
pp. 7-13
Author(s):  
Tanti Anugrah ◽  
Musadia Afa ◽  
Yolanda Fitria Syahri
Keyword(s):  
Plant Height ◽  
Cucumis Sativus ◽  
Block Design ◽  
Fruit Weight ◽  
Leaf Number ◽  
Cucumis Sativus L ◽  
Fruit Number ◽  
Randomized Block Design ◽  
Significant Difference ◽  
Flower Age

The Effect of NPK-Zeo Fertilizer on Growth and Production of Cucumber (Cucumis sativus L.)  in Iwoimopuro Village, Kolaka District. This research aimed to determine the effect of liquid fertilizer NPK-ZEO growth and result of cucumbers. This research has taken place in the village Iwoimopuro, District Wolo, Kolaka District. This research is compiled using a Randomized Block Design (RBD). Obser rations were made on plant height, leaf number, age of the plant begins to flower, age of the plant begins to bear fruit, fruit number and fruit weight. The data were processed using the Finger Print Car List (Anova) followed by Least Significant Difference Test (LSD). The results showed that administration of NPK-ZEO significant effect on plant height, leaf number, age of the plant begins to flower, age of the plant begins to bear fruit, fruit number and fruit weight

scholarly journals Aplikasi Pemupukan Terhadap Pertumbuhan Tanaman Aren Genjah (Arenga pinnata) dan Tanaman Kakao (Theobroma cacao) Pada Penanaman Sistem Jalur

2019 ◽  
Vol 7 (2) ◽  
pp. 34
Author(s):  
Yetti Elidar
Keyword(s):  
Plant Height ◽  
Block Design ◽  
Crop Productivity ◽  
Fertilizer Treatment ◽  
East Kalimantan ◽  
Palm Trees ◽  
Length Increase ◽  
Significant Difference ◽  
Early Maturing ◽  
Height Increase

Planting with a lane system in palm sugar and cacao plants is a technique of plantation cultivation that aims at soil conservation and microenvironment. Plant cultivation in the field needs to get a supply of fertilizer to increase crop productivity. This study aims to determine the effect of giving SP-36 and KCl fertilizer to the best growth of sugar palm and cacao plants in the planting of path systems. The research was conducted on land in Badak Mekar Village, Muara Badak District, Kutai Kartanegara Regency, East Kalimantan. The research method used a Randomized Block Design (RBD) and repeated 4 times. Factorial experiments consisted of 2 factors, as the first factor was SP-36 fertilizer (p) consisting of 3 levels: p0 = 0 kg plant-1 (control); p1 = 0.25 kg of plant-1; p2 = 0.5 kg of plant-1. The second factor was KCl (k) fertilizer consisting of 3 levels: k0 = 0 kg plant-1 (control); k1 = 0.25 kg of plant-1; k2 = 0.5 kg of plant-1. The variables observed included: (1) Arenaceous plants: increase in caudal circumference, increase in the fifth midrib length, increase in the number of midribs, increase in number of leaflets on the fifth midrib and (2) Cocoa plants: increase in plant height, increase in stem circumference, the number of cocoa fruits, the intensity of pest and disease attacks on cocoa fruit. Observations on each variable were carried out at 3 and 6 months after treatment (mat). Data were analyzed by variance and if there were significant differences, it was followed by the Smallest Significant Difference Test (LSD) at the level of 5%. The results of the SP-36 fertilizer treatment showed very significant differences in all observation variables in early maturing palm trees and aged 3 bsp and 6 bsp cacao plants. The treatment of SP-36 fertilizer at a dose of 0.5 kg plant-1 (p2) gave the best growth of early maturing sugar palm plant 6 in the increase of the fifth midrib length, increase in bulb circumference, increase the number of midribs and increase the number of leaflets on the fifth midrib - each 137.17 cm, 25.58 cm, 3.25 strands and 39.08 strands. Likewise in the age of 6 bsp cocoa plants SP-36 fertilizer with a dose of 0.5 kg plant-1 (p2) gave the best growth in plant height increase, increase in stem circumference and number of cocoa fruit, each 18.92 cm, 8.25 cm and 25.25 pieces. The treatment of KCl fertilizer and the interaction between SP-36 and KCl fertilizers showed no significant difference in all observation variables in early maturing and cacao plants. Observations of pest and disease attacks on cocoa fruit with the intensity of pest attacks 0.00-23.47% were included in the category of minor damage and the intensity of disease attacks 0.00-66.30% which fall into the category of moderate damage.

scholarly journals Salinity-Induced Alterations in the Growth, Membranes and Osmolytes of Musa spp. cv. Caipira (AAA) During Micropropagation

2019 ◽  
Vol 11 (6) ◽  
pp. 171
Author(s):  
Yuri Lima Melo ◽  
Isabele Aragão Gomes Trindade ◽  
Monique Cristina Simão Lopes ◽  
Cibelley Vanúcia Santana Dantas ◽  
Josemir Moura Maia ◽  
...  
Keyword(s):  
Survival Rate ◽  
Plant Height ◽  
Exposure Time ◽  
Root Length ◽  
Growth Traits ◽  
In Vitro Rooting ◽  
Ms Medium ◽  
Musa Spp ◽  
Number Of Leaves

The aim of this study was to determine the concentration and exposure time to NaCl suitable for the micropropagation of banana, through the analysis of growth traits. Banana propagules were inoculated in MS medium with different concentrations of NaCl (0; 50; 75 and 100 mM) for 120 days (multiplication and rooting, 60 days each), with monthly subcultures. These propagules were measured for plant height, number of leaves, sprouting rate, average number of formed propagules, rooting rate, root length and survival rate. After 30 days, NaCl reduced sprouting rate at multiplication; the number of leaves, rooting rate and root length in rooting; and the height and propagules number in both phases. After 60 days, the NaCl affected the sprouting rate and propagules number in the multiplication; length of root in rooting; and the height and number of leaves in both phases. After 120 days, the reduction in the survival rate was proportional to the increase of NaCl in the medium. Thus, it is concluded that NaCl reduces most of the growth traits and the treatments with 75 and 100 mM NaCl affected multiplication and in vitro rooting more intensely.

scholarly journals Effect of Dose and Compound Fertilizer on Growth and Results of Red Chili

2021 ◽  
Vol 2 (1) ◽  
pp. 5
Author(s):  
Burlian Hasani ◽  
Fitri Yetty Zairani
Keyword(s):  
Plant Height ◽  
Block Design ◽  
Dry Weight ◽  
Fruit Weight ◽  
Growth And Yield ◽  
Hot Pepper ◽  
Pepper Plant ◽  
Fertilizer Plant ◽  
Compound Fertilizer ◽  
Significant Difference

Effect of Dose and compound Fertilizer on the Growth and Yield of Hot Pepper plant (Capsicum annum, L.). This study aims to determine the effect of Formula Application and dosage of complete compound fertilizer on the growth and yield of chili plants. This study used a factorial Randomized Block Design (RAK) with nine treatment combinations and three replications, each treatment consisting of 3 plants. The combination of these treatments used two Complete Compound Fertilizer Formulas (P). Formula 1 (P1) Complete Compound Fertilizer Pril (PMLP), 2 (P2) : Complete Compound Fertilizer Tablets (PMLT), and 3 (P3) as a control mixture of single fertilizer Urea, SP 36, and KCL ( 1:1:1). The second formula is fertilizer dosage (D) which consists of three levels, namely D1 (30 g fertilizer/plant), D2 (40 g fertilizer/plant), and D3 (50 fertilizer/plant). The variables observed in this study were plant height, number of fruit per plant, fruit weight per plant, dry weight of plant stover. Based on the results of the research that has been carried out, it is concluded that the treatment of Complete Compound Fertilizer Formula (P) has a very significant effect on all observed variables, namely: plant height, number of fruits per plant, the weight of dry root, and weight of fruit per plant. While the Fertilizer Dosage (D): 30, 40, and 50 g did not show a significant difference in the results for all observed variables, and the interaction between the two did not show significant differences in all observed variables.

scholarly journals Pengaruh Biochar dan Pupuk Organik Cair dari Air Cucian Beras Terhadap Pertumbuhan dan Produksi Selada

2021 ◽  
Vol 17 (1) ◽  
pp. 76-82
Author(s):  
Deno Okalia ◽  
Tri Nopsagiarti ◽  
Gusti Marlina
Keyword(s):  
Plant Height ◽  
Block Design ◽  
Organic Fertilizer ◽  
Growth And Yield ◽  
Leaf Number ◽  
Household Waste ◽  
Vegetable Crops ◽  
Growing Media ◽  
Randomized Block Design ◽  
Significant Difference

Growing vegetable crops such as lettuce, on Ultisol soil has several obstacles, such as insufficient organic matter and nutrients in the soil. The provision of biochar and POC leri or organic fertilizer from rice washing liquid is expected to be a long-term and sustainable solution. This study was intended to test the addition of biochar to the growing media and the application of leri on lettuce cultivation. This study used a factorial Randomized Block Design (RAK) method. The first factor was the application of biochar to the planting medium (M), which consisted of M1: Ultisol without biochar and M2: Ultisol + 30 g biochar. The second factor was the administration of liquid organic fertilizer (LOF) leri (P), with 5 treatments consisting of P0: without giving LOF leri, P1: giving LOF leri without fermentation once a week, P2: giving LOF leri without fermentation twice a week, P3: giving fermented LOF leri once a week, P4: giving fermented LOF leri twice a week. The results of this study were tested statistically by analysis of variance, with the F test; if there was a significant difference, it was continued with the BNJ test (Honest Significant Difference) at a 0.05 level. The results showed that the best treatment of LOF leri in increasing lettuce growth and yield was 25% fermented LOF leri given 2 times a week, at 250 mL per lettuce plant. As a single treatment the best growing media was M2 (Ultisol soil + 30 g of biochar) with a plant height of 25.80 cm and the leaf number of 12.83; whereas the best LOF leri treatment was P4 (fermented LOF leri given twice a week), with a plant height of 30.27 cm and leaf number of 14.42. Based on whole plant fresh weight, the best treatment was the M2P4 (Ultisol + biochar growing medium and fermented LOF leri given week), which was 185.99 g per plant. Keywords: household waste, lettuce, POC leri, rice washing liquid   ABSTRAK Melakukan budidaya tanaman sayuran, seperti selada, pada tanah Ultisol memiliki beberapa kendala, seperti kurangnya bahan organik dan unsur hara pada tanah tersebut. Pemberian biochar dan POC leri atau pupuk organik dari cairan cucian beras diharapkan dapat menjadi solusi yang bersifat jangka panjang dan berkelanjutan. Penelitian ini dimaksudkan untuk menguji penambahan biochar pada media tanam dan pemberian leri pada budidaya selada. Penelitian ini menggunakan metode Rancangan Acak Kelompok (RAK) faktorial. Faktor pertama adalah pemberian biochar pada media tanam (M), yang terdiri dari M1: Ultisol tanpa biochar dan M2: Ultisol + 30 g biochar. Faktor kedua adalah pemberian pupuk organic cair (POC) leri (P), dengan sebanyak 5 perlakuan yang terdiri dari adalah P0: tanpa pemberian POC leri, P1: pemberian POC leri tanpa fermentasi 1 kali seminggu, P2: pemberian POC leri tanpa fermentasi 2 kali seminggu, P3: pemberian POC leri fermentasi 1 kali seminggu, P4: pemberian POC leri fermentasi 2 kali seminggu. Hasil penelitian ini diuji secara statistik dengan analisis ragam, dengan uji F; bila terdapat perbedaan nyata dilanjutkan dengan uji BNJ (Beda Nyata Jujur) pada taraf 0,05. Hasil penelitian menunjukkan perlakuan POC leri yang terbaik dalam meningkatkan pertumbuhan dan produksi selada adalah 25% POC leri fermentasi yang diberikan 2 kali seminggu, sebanyak 250 mL per tanaman selada. Secara tunggal perlakuan media tanam terbaik adalah M2 (tanah Ultisol+ 30 g biochar) dengan tinggi tanaman 25,80 cm dan jumlah daun 12, 83 helai; sedangkan perlakuan POC leri terbaik adalah P4 (POC leri fermentasi yang diberikan 2 kali seminggu), dengan tinggi tanaman 30,27 cm dan jumlah daun 14,42 helai. Berdasarkan berat basah tanaman, yang terbaik adalah pada perlakuan M2P4 (media tanam Ultisol + biochar dan POC leri fermentasi yang diberikan 2 kali seminggu), yaitu 185,99 g per tanaman. Kata kunci: cairan cucian beras, limbah rumah tangga, POC leri, selada

scholarly journals Comparative Assessment of Yield Performance of Neglected Cocoyam (Colocasia esculeta (L.) Schott) Parts as Planting Materials in the South Western Nigeria

2019 ◽  
pp. 32-37
Author(s):  
Ogunkunle Tajudeen ◽  
Oshagbemi H. O. ◽  
Gidado R. S. M. ◽  
Adenika O. F. Aruleba R. D.
Keyword(s):  
Plant Height ◽  
Block Design ◽  
Growth And Yield ◽  
Yield Potential ◽  
The South ◽  
Yield Performance ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Mean Differences ◽  
Planting Materials

Hitherto, there is scarcity of information on the usefulness of neglected small corms and cormels of cocoyam in the South West Nigeria. A 9 (nine) months trial was conducted to compare the growth and yield performance of different types of cocoyam planting materials with treatment consisting of T1; sprouted small cocoyam cormels, T2, trimmed out lower parts of harvested cocoyam corm and T3; moderate sized corm of 50-100g (control) arranged in a Randomised Complete Block Design (RCBD) with three replicates. Data on the number of leaves and plant height was taking at 4 week interval for 24 weeks while data on number and weight of harvested cormels was taking at 9 months after planting (MAP). The data collected were subjected to a univariate General Linear Model (GLM) two ways Analysis of Variance (ANOVA) using IBM SPSS software statistical package 21. Significance mean differences were separated using Duncan Multiple Range Test (DMRT) at 0.05 level of significance. The result showed that the highest mean number of leaves and plant height was obtained from treatment T1 (10.22) and T2 (92.83) respectively at 24 weeks after planting (WAP). While both treatment T1 and T3 collectively had the highest number of consumable cormels (54.0) in which treatment T3 weighed the highest (3.202 kg). There was no significant difference (P>0.05) in both the number of leaves produced and plant height among the different cocoyam planting materials utilised at 24 (WAP). Similarly, there was no significant difference (P>0.05) in the number and weight of cormels produced by the different treatments at 9 months after planting (MAP). The study therefore concluded that both sprouted small cocoyam cormels, trimmed out corms during planting compared favourably with moderate sized corm of weight 50-100g commonly used as planting material in term of yield potential and therefore recommended to be schedule for demonstration to cocoyam farmers in the study area for adoption.

scholarly journals Potassium Silicate, Against Water Stress, in Sweet Corn Plant Growth Traits

2019 ◽  
Vol 11 (5) ◽  
pp. 172
Author(s):  
Ausbie Luis Graça Araújo ◽  
Amanda Maria de Almeida ◽  
João de Jesus Guimarães ◽  
Fernando Soares de Cantuário ◽  
Leandro Caixeta Salomão ◽  
...  
Keyword(s):  
Water Stress ◽  
Soil Water ◽  
Plant Height ◽  
Root Length ◽  
Sweet Corn ◽  
Block Design ◽  
Potassium Silicate ◽  
Stem Diameter ◽  
Corn Plant ◽  
Corn Plants

Water stress in sweet corn plants due effect of climatic events, such as El Ni&ntilde;o, is difficult to monitor, leading to considerable losses. Silicon (Si) as an exogenous resistance elicitor may reduce water stress effects. The relationship between sweet corn plant age and its development, under induced water stress and leaf potassium silicate applications were evaluated. This work was carried out with the hybrid Tropical Plus&reg;, in a randomized factorial block design with 15, 30, 45 and 60 kPa as soil water tensions in plots and potassium silicate doses (0, 6, 12 and 24 L ha-1) in subplots. Stem diameter, plant height and leaf number per plant were evaluated at 30, 45, 60, 75 and 90 days after seeding. Root length was measured on the 90th day after seeding. Sweet corn plants submitted to water stress conditions and Si application showed an age-dependent response. Water stress did not decrease stem diameter, plant height and number of leaves per plant sprayed with Si. Root length was longer with 60 kPa soil water tension. Silicon reduced negative impacts of water stress on sweet corn plants.

scholarly journals Effect of cutting position and vine pruning level on yield of Sweet Potato (Ipomoea Batatas L.)

1970 ◽  
pp. 01-05
Author(s):  
Ncube Netsai ◽  
Mutetwa Moses, Mtaita Tuarira
Keyword(s):  
Sweet Potato ◽  
Root Length ◽  
Ipomoea Batatas ◽  
Block Design ◽  
Root Diameter ◽  
Root Weight ◽  
Stem Cuttings ◽  
Storage Root ◽  
Storage Roots ◽  
Significant Difference

There is significant variation in yield of storage roots and vines of sweet potato (Ipomoea batatas) among farmers due to use of different cutting positions and pruning of vines at different levels. This study was carried out to establish the cutting position and the vine pruning level that give the best yield of both the storage roots and vines. The study was conducted in a 3x3 factorial arrangement in Randomized Complete Block Design (RCBD) with three replications. Treatments included cutting position at three levels (apical cutting, middle cutting and basal cutting) and pruning at three levels, 0%, 25% and 50% respectively. Pruning was done. 50 days after planting. And storage root harvesting was done 100 days after planting. The two measurements were summed up to give the total vine weight. Storage root length, diameter and weight were measured at 100 DAP. Storage root length indicated significant difference (P<0.05) only among cutting positions with highest mean length (16.20 cm) obtained from apical cutting and the lowest (11.98 cm) from basal cutting. Storage root diameter, storage root weight and vine weight indicated significant interaction (P<0.05) of cutting position and vine pruning level. Highest mean root diameter and root weight were obtained from middle cutting and 25% vine pruning level, with the lowest being obtained from basal cutting and 50% vine pruning level. Highest vine weight was recorded from middle cutting and 50% vine pruning level, with the lowest being recorded from basal cutting and 0% vine pruning level. Both middle and apical stem cuttings can be recommended for higher storage root and vine yield. Vine pruning at 25% can be adopted for higher storage root yield while pruning at 50% can be suggested for higher vine yield.

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