scholarly journals Phytopharmceutical Standardization of Leaves of Jatropha tanjorensis J. L. Ellis & Saroja. (Euphorbiaceae)

2020 ◽  
pp. 1-10
Author(s):  
Romanus A. Umoh ◽  
Uwemedimo F. Umoh ◽  
Imoh I. Johnny ◽  
Omodot T. Umoh ◽  
Victor U. Anah ◽  
...  
Keyword(s):  
Glass Container ◽  
Water Soluble ◽  
Angle Of Repose ◽  
Plant Sample ◽  
Standard Procedures ◽  
Anomocytic Stomata ◽  
Microscopic Features ◽  
Microscopic Studies ◽  
Bulk Volume ◽  
Tapped Density

Jatropha tanjorensis J.L. Ellis & Saroja. (Euphorbiaceae) is a shrub commonly used as an edible vegetable and is also used as a tonic herb. The study was aimed to evaluate pharmacognostic parameters of Jatropha tanjorensis leaves. The plant leaves were collected, air-dried, pulverized and stored in a clean glass container. Standard procedures were employed to obtain the microscopic features of the fresh and powdered samples, micromeritic, chemomicroscopy, fluorescence properties, moisture content, ash values and soluble extractive values were also carried out. The results of the microscopic studies using the fresh and powered leaf samples revealed the presence of anomocytic, anomalous and paracytic stomata on the abaxial surface and anomocytic stomata on the adaxial surface. The plant sample also possessed unicellular trichomes. Results of micromeritic properties of the powdered samples show bulk volume of 38.67±0.7, tapped volume of 30.00±0.4, bulk density of 0.26±0.00, tapped  density of 0.33±0.00, angle of repose of 350, Carr’s Index of 22.96±2.15, Hausner’s ratio of 1.27±0.03, pH of 7.51 and 7.52 when hot and cold respectively. Chemomicroscopy studies revealed the presence of lignin, mucilage, calcium oxalate crystals, starch and oil in the powdered leaf. Results for moisture contents was 18.33±0.01% w/w, total ash value was 9.33±0.00%w/w, acid-insoluble ash value was 0.67±0.01%w/w, water-soluble ash value was 4.0±0.00%w/w and sulfated ash value was 14±0.01%w/w. Results for ethanol-soluble extractive value was 15±0.00%w/w, methanol-soluble extractive value was 19±0.00%w/w and water-soluble extractive value was 27±0.01%w/w. In conclusion, the above evaluation and parameters could be used to establish pharmacopoeial standard of both fresh and powdered drug of Jatropha tanjorensis.

scholarly journals Phytopharmacognostic Standardization of the Leaves of Buchholzia coriacea Engler. (Capparidaceae)

2020 ◽  
pp. 33-42
Author(s):  
Romanus A. Umoh ◽  
Uwemedimo F. Umoh ◽  
Imoh I. Johnny ◽  
Anwanabasi E. Udoh ◽  
Victor U. Anah ◽  
...  
Keyword(s):  
Drug Product ◽  
Glass Container ◽  
Water Soluble ◽  
Angle Of Repose ◽  
Powdered Sample ◽  
Standard Procedures ◽  
Anomocytic Stomata ◽  
Microscopic Features ◽  
Bulk Volume ◽  
Tapped Density

Buchholzia coriacea Engl. is a medicinal plant belonging to the family Capparidaceae. It has antimicrobial, anthelmintic, antidiabetic and antihypercholesterol activities.  The aim of this study was to employ the quality control parameters in the evaluation of the leaf of B. coriacea. The plant leaves were collected, air dried, pulverized and stored in a clean glass container. Standard procedures were carried out to obtain the microscopic features of the fresh and powdered samples, micromeritic, chemomicroscopy, fluorescence properties, soluble extractive values, moisture contents and ash values.  The results of the microscopic study using fresh and powdered leaf samples revealed the presence of anomocytic stomata on the abaxial surface (hypostomatic) with mean length of 31.8 ± 0.6 cm. The cell shape was polygonal and straight anticlinal cell wall pattern. Results of the micromeritic properties of the powdered sample showed bulk volume of 31.00± 0.70cm, tapped volume of 25.1± 0.20 cm, bulk density of 0.32 ± 0.01g/m, tapped density of 0.39± 0.01g/ml, flow rate of 2.2 ± 0.08g/s, angle of repose of 26.1 ± 1.3 degrees, Carr’s index of 18.9± 1.35 %, Hausner’s ratio of 1.23 ± 0.02, pH of 8.0 when cold and 8.2 when hot for the powdered sample liquid extract. The results of the chemomicroscopic study revealed the presence of lignin, mucilage, calcium oxalate crystals, oil, calcium carbonate, but starch was absent. Results for the ethanol-soluble extractive value was 7 ± 0.00%    , water-soluble extractive value was 14 ± 0.00%   and methanol-soluble extractive value was 3 ± 0.00%  for the powdered samples. Results for the moisture content was 10.3 ± 0.00% , total ash values was 6.3± 0.00% , acid-insoluble ash value was 1 ± 0.00% , water-soluble ash value was 3±0.00%  and sulfated ash values was 7.5 ± 0.00% . In conclusion, the above evaluation methods and parameters there in could be used to identify and authenticate both the fresh and powdered crude drug product of Buchholzia coriacea.

scholarly journals Micromorphological and Pharmacognostic Studies of Leaf and Stem of Solenostemon monostachyus P. Beauv (Lamiaceae)

2021 ◽  
pp. 230-240
Author(s):  
Romanus A. Umoh ◽  
Imoh I. Johnny ◽  
Anwanabasi E. Udoh ◽  
Nsima A. Andy ◽  
Affiong C. Essien ◽  
...  
Keyword(s):  
Water Soluble ◽  
Control Parameters ◽  
Plant Leaves ◽  
Calcium Oxalate Crystals ◽  
Total Acid ◽  
Drug Products ◽  
Standard Procedures ◽  
Quality Control Parameters ◽  
Microscopic Features ◽  
Microscopic Studies

Solenostemon monostachyus P. Beauv (Lamiaceae), it’s ethnomedical uses include anti-plasmodial, anti-pyretic, antiulcerogenic, antioxidant, anti-inflammatory and anti-nociceptive activities. The aim of this study was to employ the quality control parameters in the evaluation of the leaf and stem of Solenostemon monostachyus to aid in the identification and standardization of the medicinal plant. The plant leaves and stems were collected, identified, air-dried, pulverized and stored in separate glass containers. Standard procedures were employed to obtain the microscopic features of the fresh and powdered samples, micromeritic, chemomicroscopy, fluorescence properties, moisture contents, ash values and soluble extractive values. The results of the microscopic studies using fresh and powdered leaf samples revealed the presence of diacytic stomata on both the abaxial and adaxial surfaces (amphistomatic), with stomatal index of 27.9% and 14.8% respectively. The result of the micromeritics properties of the powdered leaf and stem samples showed angles of repose of 38.0o and 46.0o, Carr’s index of 23.7% and 32.5% and Hausner’s ratios of 1.3 and 1.5 respectively. Results for the moisture content, total, acid-insoluble and water-soluble ash values were 11.7%w/w, 13.7%w/w, 1.8%w/w and 9.4%w/w for the leaf and 13.3%w/w, 17.3%w/w, 1.8%w/w and 9.2%w/w for the stem respectively. Extractive values for water-soluble, methanol-soluble and ethanol-soluble were 26.5%w/w, 32.3%w/w, 14.5%w/w and 15.5%w/w, 15.8%w/w and 14.5%w/w for the leaf and stem respectively. Chemomicroscopy indicated the presence of lignin, calcium oxalate crystals and protein in the leaf and stem. The results obtained therefore could be used to establish pharmacopoeial standard for the fresh and powdered drug products of Solenostemon monostachyus, thus preventing adulteration.

ANTIMICROBIAL AND POWDER CHARACTERIZATION OF HERBAL DENTIFRICES

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 39-47
Author(s):  
V. K Sharma ◽  
◽  
B. Mazumder ◽  
P. P. Sharma
Keyword(s):  
Oral Cavity ◽  
Physicochemical Properties ◽  
Antimicrobial Effect ◽  
Physicochemical Characteristics ◽  
Water Soluble ◽  
Particle Rearrangement ◽  
Bulk Volume ◽  
The Impact ◽  
Tapped Density

The consumption of edible products strongly recommends the regular hygiene of oral cavity. Various dental products of allopathic and herbal origin are used as dentifrices. The dentifrices are considered safe and effective in terms of cleansing effect of oral cavity and antimicrobial effect against microbes causing bad smell and diseases such as gingivitis, pyorrhea etc. These characteristics of preparations are basically related to physicochemical properties of ingredients present in their composition and some how on directions of their use. In the present study, the marketed allopathic dentifrices coded as Brand I and II and herbal tooth powders coded as Brand III and IV were selected to analyze the impact of physicochemical properties of incorporated ingredients on their cleansing efficiency. The physicochemical characteristics studied were pH, bulk volume, tapped volume, tapped density, bulk density, true density, porosity, flowability, compressibility, compactability, cohesiveness, dispersability, Carr’s index, Hausner’s ratio, water soluble content, alcohol soluble content, foaming index, particle rearrangement behaviour and particle rearrangement constant. The antimicrobial effect of these powders was studied against Staphylococcus sorbinus, Staphylococcus salivarius and Lactobacillus acidophilus. It was observed that some of the physicochemical properties of all powders were different from each other. Marked antimicrobial effect of tooth powders was observed against pathogens. In all preparations, remarkable foaming index was analyzed that was generally considered responsible for cleansing effect.

scholarly journals Phytopharmacognostic Evaluation of the Leaves OF Gnetum africanum Welw (Gnetaceae)

2020 ◽  
pp. 32-41
Author(s):  
Romanus A. Umoh ◽  
Uwemedimo F. Umoh ◽  
Imoh I. Johnny ◽  
Omodot T. Umoh ◽  
Victor U. Anah ◽  
...  
Keyword(s):  
Moisture Content ◽  
Water Soluble ◽  
Angle Of Repose ◽  
Stomatal Index ◽  
Calcium Oxalate Crystals ◽  
Abaxial Surface ◽  
Standard Procedures ◽  
Root Stock ◽  
Leaf Powder ◽  
Stomatal Number

Background: Gnetum africanum Welw (Gnetaceae) also called African salad and Afang in Ibibio language is an evergreen, perennial, shade-tolerant vine with woody stems which can climb up to 12m or more from a tuberous root-stock. It has culinary and medicinal importance. Objective: The aim of this study was to evaluate pharmacognostic parameters of Gnetum africanum. Methods: The leaves were identified, collected, air-dried, pulverized, weighed and subjected to the evaluation of its microscopy, micromeritics, chemomicroscopy, fluorescence, soluble extractive values, moisture content and ash values using standard procedures. Results: The results obtained from microscopy revealed that the leaf has brachyparacytic, stomata, 3-5 armed and stellate trichromes on the abaxial surface. The epidermal cell wall pattern was undulate on the abaxial surface and sinuous on the adaxial surface. Stomatal number was found to be 3.1 ± 0.25 on the abaxial surface and Stomatal index was found to be 16.8% on the abaxial surface. The micromeritics analysis of the leaf powder revealed passable flow with the angle of repose of 420. The result of chemomicroscopy of the leaf revealed the presence of mucilage, lignin, calcium oxalate crystals, starch and oil. For water-soluble extractive value, the result was 13.25%w/w, methanol-soluble extractive value 4.25%w/w, ethanol-soluble extractive value 4%w/w, moisture content 10.5%w/w, total ash value was 5%w/w, acid-insoluble ash value 1%w/w, water-soluble ash value 2%w/w and sulfated- ash value 6% w/w. Conclusion: The results obtained from the pharmacognostic study provides information for the identity, quality and purity of Gnetum africanum.

Exploration of Neem Gum, Acrylamide Grafted Neem Gum and Carboxymethylated Neem Gum as Retardant in Tablet Formulation

2020 ◽  
Vol 16 (9) ◽  
pp. 1404-1410
Author(s):  
Rishabha Malviya
Keyword(s):  
Diclofenac Sodium ◽  
Tablet Formulation ◽  
Angle Of Repose ◽  
Wet Granulation ◽  
Binding Agent ◽  
Grafted Polymer ◽  
Model Drug ◽  
Tablet Dosage ◽  
Tapped Density ◽  
Derivatives Of

Background: In the previous study, investigators have synthesized acrylamide grafted and carboxymethylated derivatives of neem gum and evaluated their potential in the formulation of nanoparticles. In continuation of previous work, authors have evaluated neem gum polysaccharide (NGP), acrylamide grafted neem gum polysaccharide (NGP-g-Am) and carboxymethylated neem gum polysaccharide (CMNGP) as binding agent in the tablet dosage form. Methods: Diclofenac sodium was used as a model drug while microcrystalline cellulose and talc were used as excipient in the preparation of granules employing wet granulation technique. NGP, NGP-g-Am and CMNGP were utilized as binding agent in the preparation of granules. Prepared granules were characterized for various pre-compression and post-compression parameters. Results and Discussion: Binding agents were used in the concentration of 4-24%w/w. NGP incorporated granules showed more bulk density and lower values of tapped density, Carr’s index, bulkiness, Hausner’s ratio and angle of repose as compared to NGP-g-Am consisting granules. NGP-g-Am consisting tablets showed more hardness and zero friability as compared to NGP based tablets. Drug content was found lower for the tablets having grafted polymer in place of NGP. CMNGP were also utilized to prepare granules but granules were not be able to compress keeping all the compacting parameters same as used in the case of NGP and NGP-g-Am consisting granules. NGP and NGP-g-Am were able to sustain drug release up to 6 and 8 h, respectively. Conclusion: It can be concluded that NGP-g-Am induces better properties when used as a binder in the tablet formulation than native polymer, while CMNGP cannot be utilized as a binding agent in the preparation of a tablet.

A Scanning Electron Microscopic Study of the Normal Human Stapes

1979 ◽  
Vol 88 (6_suppl4) ◽  
pp. 2-14 ◽  
Author(s):  
Malcolm D. Graham ◽  
Rodney Perkins
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Microscopic Features ◽  
Microscopic Studies ◽  
Normal Human ◽  
Temporal Bones ◽  
Scanning Electron

The structure of the normal human stapes was studied with the scanning electron microscope. Specimens were obtained 48 hours after death from adult human temporal bones free from obvious inflammatory disease. The specimens were fixed, dissected, critical-point dried and coated with gold. In this scanning electron microscopic study an attempt has been made to systematically demonstrate the average scanning electron microscopic features of various areas of the normal human stapes. An emphasis has been placed upon demonstrating as clearly as possible the details previously unclear or unrecognized and duplication of many excellent earlier light and electron microscopic studies has not been attempted. The typical appearance of the stapes head, neck, arch, crura and footplate has been presented. It is apparent that there exists a high degree of structural specialization particularly in the stapes arch and footplate area.

scholarly journals FORMULATION AND EVALUATION OF FLURBIPROFEN FAST DISINTEGRATING TABLETS USING NATURAL SUPERDISINTEGRANTS

2016 ◽  
Vol 9 (6) ◽  
pp. 247 ◽  
Author(s):  
Mohammed Sarfaraz ◽  
Surendra Kumar Sharma
Keyword(s):  
Drug Release ◽  
Angle Of Repose ◽  
Lepidium Sativum ◽  
Disintegration Time ◽  
Natural Sources ◽  
Weight Variation ◽  
Wetting Time ◽  
Tapped Density ◽  
Fast Disintegrating Tablets

ABSTRACTObjective: The main objective of this research was to formulate Fast disintegrating tablets of Flurbiprofen incorporating superdisintegrants, isolated from natural sources like Plantago ovata (PO) seeds, Lepidium sativum (LS) seeds and agar-agar.Methods: Superdisintegrants were isolated from their natural sources using reported methods. Swelling index and hydration capacity was determined for the natural superdisintegrants to know their disintegration capacity. The tablet formulations were designed using isolated natural superdisintegrants. The powder blends were evaluated for pre-compressional parameters like angle of repose, bulk density, tapped density, carr’s index, and hausner’s ratio. Fast disintegrating tablets were prepared by direct compression method. The compressed tablets were characterized for post compression parameters.Results: All formulations had hardness, friability, weight variation and drug content within the pharmacopoeial limits. The wetting time was 84 to 254 sec, in vitro disintegration time was between 59.2 to 221 sec, and in-vitro drug release was as low as 11.80% (LS1) to a maximum of 98.99% (PO4) after 4 min of study. Among all, optimized formulation was PO4, as it showed good wetting time (84 sec), fastest disintegration time (59.2 sec), dispersion time (135 sec) and drug release of 98.99.% within 4 min.Conclusion: Flurbiprofen FDT’s were successfully developed using isolated natural disintegrants. The natural disintegrants isolated showed promising results and can prove as effective alternative for synthetic disintegrants.

scholarly journals Improvements of glycol methacrylate. I. Its use as an embedding medium for electron microscopic studies.

1977 ◽  
Vol 25 (3) ◽  
pp. 163-174 ◽  
Author(s):  
R C Spaur ◽  
G C Moriarty
Keyword(s):  
Electron Beam ◽  
Water Soluble ◽  
Cross Linking ◽  
Electron Microscopic ◽  
Glycol Methacrylate ◽  
Ultrastructural Studies ◽  
Tissue Morphology ◽  
Methacrylate Monomer ◽  
Microscopic Studies ◽  
Embedding Medium

The technique for using the water-soluble embedding medium glycol methacrylate has been improved for ultrastructural studies by the simplification of the method of formation of prepolymers used in embedding the tissue, by the addition of a cross-linking agent so that sections are stable in the electron beam, and by improving the softness of the blocks by the addition of a plasticizing agent. The preservation of tissue morphology has been improved by complete dehydration in glycol methacrylate monomer prior to infiltration with the prepolymer. Preservations of tissue morphology is further enhanced by complete dehydration in ethanols and embedding in the improved glycol methacrylate medium.

scholarly journals Phyto Pharmacognostic Study of Lagerstroemia speciosa - An Analytical Study

2020 ◽  
Vol 5 (05) ◽  
pp. 206-213
Author(s):  
Jyothi S. Murthy ◽  
B. R. Lalitha ◽  
Aahalya Sharma
Keyword(s):  
Analytical Study ◽  
Microscopical Study ◽  
Water Soluble ◽  
Lower Epidermis ◽  
Calcium Oxalate Crystals ◽  
Phytochemical Study ◽  
Lagerstroemia Speciosa ◽  
Anomocytic Stomata ◽  
Leaf Powder ◽  
Sugar Levels

Lagerstroemia speciosa (L.) Pers (Hindi - Jarul, Taglog - Banaba) a member of Lythraceae family is found all over India, specially in Bengal, Assam and Deccan Peninsula. The leaves of L. speciosa is widely used for lowering blood sugar levels in Philippines, Japan and Taiwan. Pharmacognostical investigation of leaves of L. speciosa was done by evaluating its morphological, microscopical studies, Physicochemical and phytochemical parameters. Rasa Nirdharana was done. Microscopical study revealed presence of epidermal cells contained clusters of rosette aggregate calcium oxalate crystals and few cells were mucilaginous. Lower epidermis showed the presence of anomocytic stomata. Physical constants of leaf powder showed, loss on drying - 3.8%, total ash - 6.7%, acid insoluble ash - 1.039%, water soluble extractive value - 8.88%, alcohol soluble extractive value - 4.49% and pH - 5.75. Preliminary phytochemical study revealed the presence of alkaloids, flavonoids, saponins, triterpenes, tannins, proteins and iron. Rasa Nirdharana confirmed the leaves are Kashaya in Rasa. The Pharmacognostical study was useful for authentication of leaves of Lagerstoemia speciosa.

scholarly journals A PHYTOCHEMICAL STUDY ON EUPATORIUM GLANDULOSUM

2019 ◽  
pp. 77-80
Author(s):  
SILPA M ◽  
SURESH JOGHEE ◽  
HAMSALAKSHMI
Keyword(s):  
Ethyl Acetate ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Flavonoid Content ◽  
Plant Materials ◽  
Phytochemical Study ◽  
Aerial Parts ◽  
Standard Procedures ◽  
Soxhlet Method ◽  
Alcohol Ethyl

Objective: Eupatorium glandulosum is a useful medicinal plant belongs to the family Asteraceae. It is traditionally used to treat various diseases such as wound healing, antioxidant, and antiproliferative. The present study was aimed to investigate the physicochemical and phytochemical properties of various extracts of aerial parts of E. glandulosum. Methods: The E. glandulosum plant materials were extracted using the solvents alcohol, ethyl acetate, and chloroform by Soxhlet method. The extracts were screened for physiochemical constants, preliminary phytochemical analysis for carbohydrates, glycosides, alkaloids, flavonoids, phenols, tannins, and saponins. The quantitative phytochemical analysis was carried out for total flavonoid and total phenols using standard procedures. Results: The physiochemical constituents such as total ash, acid-insoluble ash, and water-soluble ash were found to be 14.25% (w/w), 5% (w/w), and 7.30% (w/w), respectively. The preliminary phytochemical screening confirmed the presence of carbohydrates, glycosides, alkaloids, flavonoids, phenols, tannins, and saponins. The flavonoid content of the plant extracts was found to be in the descending order ethyl acetate ˃alcohol ˃chloroform and the phenolic content was found to be alcohol ˃ethyl acetate ˃chloroform. Conclusion: The result showed the presence of phytochemical constituents and higher values of phenolic and flavonoid content make the plant useful for the formulation of the different drugs for human uses for treating various diseases.

Sign in / Sign up

Export Citation Format

Share Document