Comparison of Salivary Protein Profile and Dietary Habits in Children With and Without Early Childhood Caries

Background and objectives: The purpose of this study was to evaluate the relationship between the total salivary protein concentration and dietary habits in Caries-free, Early childhood Caries and Severe Early Childhood Caries children. Materials and methods: The present study included Thirty children who were divided into three groups. They were further divided into Caries-free, Early childhood Caries and Severe Early Childhood Caries with 10 children in each group. Information was collected regarding oral hygiene practices, feeding habits and dietary pattern through a face-to-face interview with the parent/caretaker of the child. Unstimulated saliva was collected and total protein concentration was estimated. Multivariate logistic regression analysis was performed to assess the relative contributions of dietary and feeding patterns between children with and without ECC. ANOVA was done to identify the difference in mean protein concentration between the groups. Results: Risk factors specific to the ECC group were Breast feeding <24 months (OR 2.1, 95% CI, 0.35-13.0), bottle feeding (21.0, 95% CI, 2.8-15.7), snacking more than 3 times per day (OR 1.22, 95% CI 0.283-5.734), consumption of less protein diet (OR 4.1, 95% CI, 1.99-8.81), consumption of less milk products (2.33, 95% CI, 1.67-6.77). Results also revealed that there was no statistically significant difference in total protein concentration between Caries-free, Early childhood Caries and Severe Early Childhood Caries group. Conclusion: Within the limitation of this study, we can conclude that, Breast feeding less than 24 months, bottle feeding, snacking more than thrice per day, consuming low protein diet and low milk products had a high risk of developing ECC. There was no significant difference in the mean protein concentration between caries free, ECC and S ECC group.

Download Full-text

Magnetic Bead-Based Salivary Peptidome Profiling Analysis for Severe Early Childhood Caries

Caries Research ◽

10.1159/000360868 ◽

2014 ◽

Vol 49 (1) ◽

pp. 63-69 ◽

Cited By ~ 8

Author(s):

Yan Si ◽

Shuang Ao ◽

Weijian Wang ◽

Feng Chen ◽

Shuguo Zheng

Keyword(s):

Mass Spectrometry ◽

Early Childhood ◽

Early Childhood Caries ◽

Expression Profiles ◽

Salivary Protein ◽

Preschool Age ◽

Diagnostic Model ◽

Peptide Mass ◽

Severe Early Childhood Caries ◽

Childhood Caries

Objective: To investigate the differential salivary protein expression profiles between children with severe early childhood caries (S-ECC) and caries-free (CF) children at the age of 3 years. Methods: We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with weak cation exchange magnetic beads, and peptide mass fingerprints were created by scanning mass spectrometry signals. Salivary samples from 20 children were analyzed (10 for each group). Results: Eleven protein peaks were significantly different (p < 0.05) between the two groups. Eight of these peaks were higher in the S-ECC group and three were higher in the CF group. To establish a diagnostic model for discrimination between the two groups, we chose three peptides (3,186.2, 3,195.8 and 3,324.8 Da) that exhibited the best fitted curve, by which the two groups were better separated when compared with other combinations. Conclusions: The salivary biomarkers identified revealed significant differences between the CF and the S-ECC group. Our results provide novel insight into the salivary protein profile of preschool-age children with dental caries and may lead to the development of a new strategy for screening high-risk populations.

Download Full-text

Incidence of Oral Candidiasis, and the Relationship of Oral Candida Species with Severe Early Childhood Caries: A Microbiological In Vivo Study

Journal of Dr Behcet Uz Children s Hospital ◽

10.5222/buchd.2020.09475 ◽

2020 ◽

Author(s):

Gülçin Bulut ◽

Yelda Sorguc

Keyword(s):

Early Childhood ◽

Candida Species ◽

Early Childhood Caries ◽

Oral Candidiasis ◽

Control Group ◽

Sabouraud Agar ◽

Significant Difference ◽

Severe Early Childhood Caries ◽

Childhood Caries

INTRODUCTION: Cariogenic microorganisms play an important role in the development of caries. Although there are many researches on Streptococcus mutans as being a primary causative factor in caries, there is a lack in evaluation of relation between oral candidiasis and severe early childhood caries (S-ECC). The purpose of this study is to determine the frequency of candida carriage, characterize different Candida species, and investigate their relationship with caries in 5-year-old children with S-ECC and with no caries. METHODS: This study was conducted on a total of 42 children aged 5-years with (Group S-ECC, n=21) and without caries (control group, n=21). Plaque samples were taken from the buccal, lingual and cervical surfaces of the teeth using a sterile cotton swab and cultured on Sabouraud Agar, Patota Agar and Chrome Agar for fungal growth. Number of candida colonies were recorded, their species were identified and germ tube test was used for virulance marker. RESULTS: The candidal carriage in the S-ECC and control groups was 52.4% and 9.5%, respectively. The intergroup difference was statistically significant (p=0.003). The incidence of C.albicans among all candida species was determined as 92%. The only isolated non-albicans Candida was C.Dupliniensis, the incidence in all candida species was 8 percent. The frequency of C.albicans was significantly higher in the S-ECC group (p=0.006). The virulence factor showed a significant difference between the groups (p=0.006). DISCUSSION AND CONCLUSION: This study shows a strong relation between the presence of candida and S-ECC. C.albicans was the most common among detected candida species.

Download Full-text

Relationship between Severe Early Childhood Caries and breast milk`s lactose among 12-24 month old children.

10.21203/rs.3.rs-142813/v2 ◽

2021 ◽

Author(s):

Hamidreza Poureslami ◽

Maryam Sharifi ◽

Mahla Vahedi ◽

Salehe Sabouri ◽

Parnian Poureslami ◽

...

Keyword(s):

Early Childhood ◽

Breast Milk ◽

Early Childhood Caries ◽

Average Level ◽

Average Amount ◽

Cross Sectional ◽

Significant Difference ◽

The Relationship ◽

Severe Early Childhood Caries ◽

Childhood Caries

Abstract Aim: The study aimed to investigate the relationship between the occurrence of Severe Early Childhood Caries (S-ECC) and breast milk lactose, in infants aged 12 to 24 months.Material and Methods: This cross-sectional descriptive-analytical study was carried out in April to July 2020 on 30 children aged 12 to 24 months with or without S-ECC who were solely breastfed Also, the mothers answered questions about their breastfeeding. After the child's dental visit, the mother was asked to express 10 to 20 ml of her milk as a sample and give it to the researchers. The samples were immediately kept at -4 ° C and then they were tested to lactose measurement. Finally, the test results were analyzed by SPSS 21 software. Results: The average amount of lactose in the breast milk of cases with S-ECC infant was 5.74g/100 ml, and the average amount of lactose in the breast milk of cases without S-ECC infant was 4.64g/100 ml. There was no significant difference in lactose concentration between the two groups (p=0/64). The average number of breastfeeding times in cases with S-ECC infants was 7.87 per day while in the healthy cases this was 7.33 per day. There was no significant difference between the numbers of breastfeeding times per day in the two groups.Conclusion: According to this study, the average level of lactose in the breast milk samples was lower than the average level of lactose in breast milk in other countries, and the amount of lactose in breast milk of children with S-ECC was slightly higher than the amount of lactose in breast milk in children with healthy teeth.

Download Full-text

Feeding practices and early childhood caries among children with primary dentition

Journal of Kathmandu Medical College ◽

10.3126/jkmc.v10i2.40017 ◽

2021 ◽

Vol 10 (2) ◽

pp. 74-79

Author(s):

Sunanda Sundas ◽

Barun Kumar Sah ◽

Neha Dhakal ◽

Amita Rai ◽

Neera Joshi

Keyword(s):

Early Childhood ◽

Early Childhood Caries ◽

Feeding Practices ◽

Bottle Feeding ◽

Caries Experience ◽

Cross Sectional ◽

Validated Questionnaire ◽

Night Feeding ◽

Significant Difference ◽

Childhood Caries

Background: Early childhood caries (ECC) is major oral health problem of young children. Risk factors for ECC are poor oral hygiene and feeding practices. Prevalence and severity of ECC is increasing with change in lifestyle and diet pattern in developing countries. Objectives: To assess the association between feeding practices and severity of early childhood caries in preschool children. Methods: An analytical cross-sectional study was conducted with convenient sample of 96 children up to six years age in Peoples Dental College from July to December 2020. Clinical examination included the recording of dental caries (dmft) using WHO criteria. The self-validated questionnaire consisting seven questions about feeding practices was asked to mothers. The Chi-square test was performed to determine association between severity of ECC and feeding pattern. Results: Mean dmft was 6.77 ± 5.91. Prevalence of severe-ECC (S-ECC) was highest in youngest age group. Caries experience was similar in breast fed, bottle fed, and mixed. There was no significant difference in non-severe ECC and ECC in relation to duration of breast or bottle feeding but frequency of night feeding was associated with S-ECC. Children given ready-made infant formula solid food had more S-ECC (13, 92%) compared to non-severe ECC (1, 7.1%) which was statistically significant. Children given homemade gram flour food were noted to have less of S-ECC (5, 27.5%) than nonsevere ECC (13, 72.5%) which was statistically significant. Conclusion: Present study revealed that multiple night feeding and weaning with readymade food are significant factors for S-ECC.

Download Full-text

Severe early childhood caries and behavioral risk factors among 3-year-old children in Lithuania

Medicina ◽

10.3390/medicina46020020 ◽

2010 ◽

Vol 46 (2) ◽

pp. 135 ◽

Cited By ~ 22

Author(s):

Eglė Slabšinskienė ◽

Simona Milčiuvienė ◽

Julija Narbutaitė ◽

Ingrida Vasiliauskienė ◽

Vilija Andruškevičienė ◽

...

Keyword(s):

Risk Factors ◽

Early Childhood ◽

Early Childhood Caries ◽

Tooth Brushing ◽

Bottle Feeding ◽

Behavioral Risk Factors ◽

Behavioral Risk ◽

Fluoride Toothpaste ◽

Severe Early Childhood Caries ◽

Childhood Caries

The objective of the present study was to investigate the prevalence and severity of early childhood caries (ECC) and severe early childhood caries (S-ECC) in 3-year-old Lithuanian children. The impact of selected behavioral risk factors on the development of S-ECC was investigated. Material and methods. A total of 950 children were examined in kindergartens. Diagnosis of dental caries followed the WHO criteria. Questionnaires were delivered to mothers of the children with S-ECC and caries-free children. The questions were related to the children’s dietary habits, temperament, oral hygiene, and the use of fluoride toothpaste. Results. The prevalence of ECC was 50.6% with a mean dmft of 2.1 (SD, 0.1) and a mean dmfs of 3.4 (SD, 0.2). The prevalence of S-ECC was 6.5% with a mean dmft of 7.8 (SD, 0.1) and dmfs of 18.1 (SD, 0.6). A significantly higher percentage of children developed S-ECC when they were breast-fed for a period longer than one year, were sleeping with a bottle containing carbohydrates during the night, or were allowed to sip from a bottle either going to sleep or during the day. A significantly higher percentage of mothers having caries-free children knew about risk factors of S-ECC and started tooth brushing after the eruption of the first tooth. There was no significant difference between the groups regarding the use of fluoride toothpaste. Conclusions. Improper infant bottle-feeding habits and no tooth brushing were found to be significant for the development of S-ECC among 3-year-old Lithuanian children.

Download Full-text

Early Childhood Caries in 3 to 5 Year Old Children in Trinidad and Tobago

Dentistry Journal ◽

10.3390/dj7010016 ◽

2019 ◽

Vol 7 (1) ◽

pp. 16

Author(s):

Tricia Percival ◽

Julien Edwards ◽

Salvacion Barclay ◽

Bidyadhar Sa ◽

Md Anwarul Azim Majumder

Keyword(s):

Early Childhood ◽

Preschool Children ◽

Trinidad And Tobago ◽

Oral Hygiene ◽

Early Childhood Caries ◽

Dietary Habits ◽

Statistical Significance ◽

Bottle Feeding ◽

Hygiene Practices ◽

Childhood Caries

Background: This study was done to evaluate the prevalence and contributory factors of early childhood caries (ECC) and severe ECC (S-ECC) among preschool children of Kindergartens and Early Childhood Centres in Trinidad and Tobago. Methods: A cross-sectional study was carried out involving 342 preschool children aged 3 to 5 years. The school staff distributed a structured questionnaire to the children to be completed by the mother. Clinical examinations were conducted by calibrated examiners. Statistical significance was set at p < 0.05 in all analyses. Results: The prevalence of ECC and S-ECC was 50.3% and 52.3%, respectively. Dietary and oral hygiene factors particularly with bottle feeding and high plaque levels were major contributors to dental caries in this population. Conclusion: ECC and S-ECC are significant issues that occur in preschool children in Trinidad and Tobago. The development of ECC and S-ECC can be attributed to certain environmental factors like dietary habits and oral hygiene practices. Early dental assessment, broad-based oral health education programmes, increased parental/guardian engagement during oral hygiene practices and greater access to facilities for early childhood caries prevention and management can help alleviate the problems of ECC and S-ECC in this population.

Download Full-text

Evaluation of Possible Associated Factors for Early Childhood Caries and Severe Early Childhood Caries: A Multicenter Cross-Sectional Survey

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4628-40.2.118 ◽

2016 ◽

Vol 40 (2) ◽

pp. 118-123 ◽

Cited By ~ 7

Author(s):

Bugra Özen ◽

AJP Van Strijp ◽

Levent Özer ◽

Hulya Olmus ◽

Aysegul Genc ◽

...

Keyword(s):

Early Childhood ◽

Associated Factors ◽

Early Childhood Caries ◽

Pediatric Dentistry ◽

Bottle Feeding ◽

Caries Experience ◽

Cross Sectional ◽

Positive Effects ◽

Significant Difference ◽

Childhood Caries

Objectives: The present study evaluated associated factors for developing early childhood caries (ECC) and Severe-ECC (S-ECC) in a group of children aged 24–71 months. Potential positive effects of early dental visit on formation of ECC is investigated as well. Study Design: This was a multicenter, cross-sectional study conducted at three governmental and university pediatric dentistry clinics in 408 preschool children who were randomly selected from a total of 4116 children. The questionnaires administered to the mothers by interview and intraoral examination performed by calibrated pediatric dentists. The children were evaluated in three groups according to their caries experience as who had caries free, ECC and S-ECC. Results: The following factors were significantly associated with caries formation: 1. Prolonged (i.e., >18 months) breastfeeding in preterm babies (OR=2.4) 2. Prolonged breastfeeding in children who started tooth brushing after 1.5 years of age (OR=3.7), 3. Sugar (p<0.001) and fruit-juice consumption (p<0.0001), and 4. Lack of periodic dental examination (p<0.05). Parental smoking habit does not significantly affect ECC development. Nocturnal bottle feeding and nocturnal feeding also affected S-ECC formation significantly (p=0.043 and p=0.005, respectively). Conclusions: There is a significant difference between the children with caries and caries-free associated with the brushing initiation age started before or after 18 months. If a child is under the risk of multiple caries factors, it is very difficult to evaluate which habits affect the caries formation or increase the severity of the caries lesions.

Download Full-text

Association between feeding habits and severe - early childhood caries in children up to 24 month old

Stomatoloski glasnik Srbije ◽

10.1515/sdj-2016-0012 ◽

2016 ◽

Vol 63 (3) ◽

pp. 117-124 ◽

Cited By ~ 2

Author(s):

Marija Obradović ◽

Olivera Dolić ◽

Jovan Vojinović ◽

Slava Sukara

Keyword(s):

Early Childhood ◽

Early Childhood Caries ◽

Dietary Habits ◽

Eating Habits ◽

Feeding Habits ◽

Cross Sectional Study ◽

Primary Tooth ◽

Cross Sectional ◽

Severe Early Childhood Caries ◽

Childhood Caries

Abstract Introduction During the first two years of life children’s nutrition is mostly based on frequent, liquid and sweetened meals which can cause Severe-Early Childhood Caries (S-ECC) development. The aim of this research was to determine the relationship between dietary habits and S-ECC in children up to 24 month-old living in Banja Luka, Bosnia and Herzegovina. Methods Cross-sectional study included representative sample of 192 children. Before dental examination of children, each parent/caregiver was interviewed about the basic info, socio-demographic characteristics and children's eating habits. The questionnaire was conducted as interview (“face to face”). Subjects were divided into two groups: the first group - children with S-ECC and the second group - caries free children. For statistical analysis and presentation of results SPSS 16.0 for Windows, MS Office Word and Microsoft Office Excel were used. Results In the study sample 34.9% of children were suffering from S-ECC. About 50% of children who were breast-fed at night after first tooth eruption had S-ECC. The use of baby bottle with milk or other sweetened content during bedtime and during the night was identified as significant caries risk factor (P <0.05). Conclusion Nighttime breastfeeding, use of bottle with milk during bedtime/nighttime or other sweetened content during night after eruption of first primary tooth were strongly associated with S-ECC in the examined children.

Download Full-text

Dental Treatments under the General Anesthesia in a Child with Keratitis, Ichthyosis, and Deafness Syndrome

Case Reports in Dentistry ◽

10.1155/2013/618468 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6

Author(s):

Sera Sımsek Derelioglu ◽

Yücel Yılmaz ◽

Sultan Keles

Keyword(s):

Early Childhood ◽

Case Report ◽

General Anesthesia ◽

Early Childhood Caries ◽

Sensorineural Deafness ◽

Oral Symptoms ◽

Severe Early Childhood Caries ◽

Childhood Caries

KID syndrome is a rare genodermatosis characterized by keratitis, ichthyosis, and sensorineural deafness. Although the dermatological, ophthalmologic, and sensorineural defects are emphasized in the literature, oral and dental evaluations are so superficial. In this case report, dental and oral symptoms of a three year and five months old boy with KID syndrome, suffering severe Early Childhood Caries (s-ECC) and dental treatments done under General Anesthesia (GA) were reported.

Download Full-text