Evaluation of Levels of Some Inflammatory Cytokines in Preeclamptic Women in Owerri

Preeclampsia is a serious and life-threatening pregnancy complication. In this study, the levels of inflammatory cytokines were measured in preeclamptic women in Owerri, Imo State. A total of 120 pregnant women aged 18-45 years at 20-40 weeks of pregnancy were recruited; 60 were preeclamptic women (test group) while 60 were normotensive pregnant women (control group). Preeclampsia was determined by the presence of ≥2+ protein in the urine using combi 2 dipstick for urinalysis and sphygmomanometer blood pressure reading of ≥ 140/90 mmHg. From the demographic data obtained in the studied subject through questionnaire, it showed that nulliparity and family history of high blood pressure were the most dominant risk factor of preeclampsia. The mean of the pro-inflammatory cytokines (IL-6 and TNF-α) in the test group were 4.33±1.42 pg/ml and 8.23±2.73 pg/ml while that of the control group were 3.38±1.22 pg/ml and 6.89±1.88 pg/ml. There was a significant increase in the pro-inflammatory cytokines of the preeclamptic women when compared to the control group. The mean of the anti-inflammatory cytokines (IL-10 & IL-4) of the group were 78.88±14.28 pg/ml and 27.92±7.22pg/ml while the control group was 91.39± 18.46 pg/ml and 31.25±6.90 pg/ml. There was a significant decrease in the result obtained from the test group when compared to the control group. The levels of the pro-inflammatory cytokines were higher than the levels of the anti-inflammatory cytokines of the test group when compared with the control group. Hence there is need to manage the disorder with an anti inflammatory drugs in order to enhance obstetric care, thereby reducing maternal mortality and morbidity that may occur from haemostatic abnormalities.

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Studies of Some Haemostatic Variables in Preeclamptic Women in Owerri, Imo State, Nigeria

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i42b32402 ◽

2021 ◽

pp. 39-48

Author(s):

Obioma Raluchukwu Emeka-Obi ◽

Nancy C. Ibeh ◽

Emmanuel Ifeanyi Obeagu ◽

Hope M. Okorie

Keyword(s):

Blood Pressure ◽

Pregnant Women ◽

Demographic Data ◽

Test Group ◽

Peripheral Circulation ◽

Control Group ◽

Imo State ◽

Significant Difference ◽

Life Threatening ◽

Abnormal Pattern

Preeclampsia is a serious and life-threatening pregnancy complication. In this study, the levels of haemostatic parameters were measured in preeclamptic women in Owerri, Imo State. A total of 120 pregnant women aged 18-45 years at 20-40 weeks of pregnancy were recruited; 60 were preeclamptic women (test group) while 60 were normotensive pregnant women (control group). Preeclampsia was determined by the presence of ≥2+ protein in the urine using combi 2 dipstick for urinalysis and sphygmomanometer blood pressure reading of ≥ 140/90 mmHg. From the demographic data obtained in the studied subject through questionnaire, it showed that nulliparity and family history of high blood pressure were the most dominant risk factor of preeclampsia. The mean haemostatic parameters (PT, APTT, Fibrinogen, D-dimer and t-PA) of the test group were 12.3±0.94sec, 32.17±3.38sec, 627.31±106.93mg/ld, 2.23±0.50mg/l, 2.65±0.57ng/ml respectively, while the control group were 11.76±0.97sec, 28.69±2.64sec, 554±124.81 mg/dl, 1.89±0.44mg/l and 2.37±0.66 ng/ml respectively. There was a significant difference between the haemostatic parameter of the test group when compared with the control group. The results indicated that although anaemia and activation of coagulation and fibrinolysis occur within the peripheral circulation of both preeclamptic and normotensive pregnancy, an abnormal pattern of haemostasis occurs more in preeclamptic women.

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EFFECTIVENESS OF LAVENDER AROMATHERAPY AND CLASSICAL MUSIC THERAPY IN LOWERING BLOOD PRESSURE IN PREGNANT WOMEN WITH HYPERTENSION

Belitung Nursing Journal ◽

10.33546/bnj.301 ◽

2017 ◽

Vol 3 (6) ◽

pp. 750-756

Author(s):

Sri Maisi ◽

Suryono Suryono ◽

Melyana Nurul Widyawati ◽

Ari Suwondo ◽

Suryati Kusworowulan

Keyword(s):

Blood Pressure ◽

Music Therapy ◽

Systolic Blood Pressure ◽

Pregnant Women ◽

Classical Music ◽

Combination Group ◽

Control Group ◽

P Value ◽

The Mean ◽

And Control

Background: Hypertension during pregnancy remains high in Indonesia. It is a major cause of maternal death. Aromatherapy lavender and classical music therapy are considered effective in lowering blood pressure in hypertension.Objective: To examine the effect of lavender aromatherapy and classical music therapy in lowering blood pressure in pregnant women with hypertension.Methods: A quasy experimental study with pretest-posttest control group design. There were 52 pregnant women with the inclusion criteria selected as samples using simple random sampling, divided into lavender aromatherapy group, classical music group, combination of aromatherapy and music group, and control group. Sphygmomanometer was used to measure blood pressure. Mann Whitney and Post Hoc test were used for data analysis.Results: Results showed that four groups have a significant decrease in systolic blood pressure after given intervention with p-value <0.05. The mean decrease of systolic blood pressure among four groups was: lavender group (5.77 mmHg), music group (7.23 mmHg), combination group (9.54 mmHg), and control group (3.67 mmHg); and the mean decrease of diastolic blood pressure was: the lavender group (2.77 mmHg), music group (0.61 mmHg), combination group (8.23 mmHg), and control group (3.42 mmHg).Conclusion: there was a significant effect of lavender aromatherapy and classical music therapy in lowering blood pressure in pregnant women with hypertension. However, the combination of both interventions was more effective than lavender aromatherapy or music therapy alone.

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Effectiveness of a family-centered behavioral and educational counselling approach to improve periodontal health of pregnant women: a randomized controlled trial

BMC Oral Health ◽

10.1186/s12903-020-01265-6 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Pei Liu ◽

Weiye Wen ◽

Ka Fung Yu ◽

Xiaoli Gao ◽

Edward Chin Man Lo ◽

...

Keyword(s):

Oral Health ◽

Pregnant Women ◽

Oral Hygiene ◽

Controlled Trial ◽

Test Group ◽

Control Group ◽

Oral Health Promotion ◽

Periodontal Health ◽

The Mean ◽

Family Centered

Abstract Background Poor oral hygiene and high hormone levels during pregnancy can lead to a deterioration in periodontal health. This study assessed the effectiveness of a family-centered behavioral and educational counselling program on improving the periodontal health of women during pregnancy and postpartum. Methods A randomized controlled trial was conducted among pregnant women (10th-22nd gestational week) and their husbands. Participating families were randomized into test and control groups. Intervention in the test group included explanation of oral health education (OHE) pamphlets, oral hygiene instruction, individualized feedback, and proposed solutions to overcome barriers in self-care. Reinforcements were implemented in the 3rd trimester of pregnancy and six months postpartum. In the control group, only OHE pamphlets were distributed. The assessed outcomes were bleeding on probing (BOP), periodontal pocket (Poc), loss of clinical attachment (LoA), and Visible Plaque Index (VPI). The data collection was carried out at baseline (T0), in the 32nd gestational week (T1), and 12 months postpartum (T2). Results Altogether 589 pregnant women were recruited, and 369 attended all three visits (test:188; control:181). In the test group, the mean VPI score at T0 was 0.19, which decreased to 0.14 at T1 and 0.15 at T2. In the control group, the mean VPI decreased from 0.19 at T0 to 0.16 at T1, but increased to 0.22 at T2. A main effect of time and intervention and an interaction between time and intervention were detected (all p < 0.05), indicating that the intervention effect differed between T1 and T2. The test group showed a significantly greater decrease over time than the control group did. Similarly, the mean BOP% decreased more significantly over time in the test group (T0:57%, T1:46%, T2:35%) than in the control group (T0:58%, T1:52%, T2:46%). For Poc and LoA, there were improvements in both study groups at 12 months postpartum, compared with during pregnancy (p < 0.001). Conclusions Providing family-centered, behavioral, and educational counselling to pregnant women at an early stage of pregnancy and with reinforcements can improve their oral hygiene and reduce gingival inflammation. The effect can be sustained over an extended period and is greater than that of distributing oral health leaflets alone. Trial registration Clinicaltrials.gov, #NCT02937194. Registered 18 October 2016. Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT02937194?cond=Family-centered+oral+health+promotion+for+new+parents+and+their+infants&draw=2&rank=1

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The Effect ofAdditional Nanoparticles Supplementation of Indonesian Bay Leaf (SyzigiumPolyanthum) on Blood Pressure in Pregnancy Hypertension

Volume 5 - 2020, Issue 9 - September - International Journal of Innovative Science and Research Technology ◽

10.38124/ijisrt20jul706 ◽

2020 ◽

Vol 5 (7) ◽

pp. 1320-1323

Author(s):

Miftah Nurlaily El Akhlaq ◽

Suharyo Hadisaputro ◽

Suhartono . ◽

Sri Sumarni ◽

M. Choiroel Anwar

Keyword(s):

Blood Pressure ◽

Data Analysis ◽

Pregnant Women ◽

Diastolic Blood Pressure ◽

Intervention Group ◽

Reduce Blood Pressure ◽

Control Group ◽

Pregnancy Hypertension ◽

The Mean ◽

In Pregnancy

Bay leaf (Syzygiumpolyanthum) or Daun Salam is one of the plants that has many benefits, one of them is to reduce blood pressure. The aim of this study is to prove the effect of adding nanoparticles supplementation of bay leaf (Syzygiumpolyanthum) to systolic and diastolic blood pressure in pregnancy hypertension.The study was quasi-experiment. 39 respondents were divided into 19 pregnant women in intervention group and 20 pregnant women in the control group. The intervention was given for 14 days by consumingnifedipine 10mg plus 80mg bay leaf nanoparticle capsules to the intervention group.The results of data analysis showed a decrease in systolic blood pressure (p = 0.000) and diastolic blood pressure (p = 0.004) when compared with the control group, which means that there were differences in the mean systolic and diastolic blood pressure between the two groups after treatment. The addition of bay leaf nanoparticle supplementation 1x80 mg for 14 days affected the decrease in systolic and diastolic blood pressure in pregnancy hypertension. So that the supplementation of bay leaf nanoparticles can be used as adjunctive therapy in pregnancy hypertension

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Lipoprotein (a) level in pre-eclampsia patients

Bangladesh Medical Research Council Bulletin ◽

10.3329/bmrcb.v36i3.7289 ◽

1970 ◽

Vol 36 (3) ◽

pp. 97-99 ◽

Cited By ~ 10

Author(s):

Saila Parvin ◽

Latifa Samsuddin ◽

Ahammed Ali ◽

Safiqul Alam Chowdhury ◽

Ibrahim Siddique

Keyword(s):

Blood Pressure ◽

Pregnant Women ◽

High Serum ◽

Serum Lipoprotein ◽

Control Group ◽

High Serum Level ◽

Lipoprotein A ◽

Control Serum ◽

The Mean ◽

And Control

This study was carried in BSMMU from July 2001 to June 2003. During the study period, 60 pregnant women were studied. Thirty patients were preeclamptic and thirty were normal healthy pregnant women served as control. Serum lipoprotein(a) was found significantly higher in preeclamptic women 56.63±22.6 mg/dl and found within limit in normal healthy pregnant women, 12.89±4.59mg/dl. Result is statistically highly significant (P<0.001). Mean Systolic Blood Pressure was 163.33±29.63 mmHg and 117.00±11.19mmHg in case and control and Diastolic Blood Pressure was 108.53±14.54 mmHg and 76.00±6.87mmHg respectively in case and control group. Result was highly significant as P <0.001. The mean (±SD) serum lipoprotein(a) concentration in normal pregnancies and preeclampsia were found to be 12.91±4.94 and 56.65±22.62. Moderate Proteinuria was found in 77.5% and severe proteinuria in 22.2% cases of preeclampsia respectively. Regardless of mechanism and pathophysiology of preeclampsia, we found high serum level of lipoprotein (a) in preeclampsia patients. These high levels of lipoprotein (a) significantly correlated with blood pressure and proteinuria. DOI: 10.3329/bmrcb.v36i3.7289Bangladesh Med Res Counc Bull 2010; 36: 97-99

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Fisetin has Inhibitory Effects on the TLR 4/NF-κB-Mediated Inflammatory Pathway After Traumatic Brain Injury in Mice: A Potential Neuroprotective Role

10.21203/rs.3.rs-776980/v1 ◽

2021 ◽

Author(s):

Chenhui Zhou ◽

Sheng Nie ◽

Zhepei Wang ◽

Fanyong Gong ◽

Jingmi Wu ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

Brain Edema ◽

Inflammatory Cytokines ◽

Nuclear Factor Κb ◽

Neuroprotective Effects ◽

Inflammatory Pathway ◽

Mortality And Morbidity ◽

Anti Inflammatory ◽

Pro Inflammatory Cytokines

Abstract Inflammatory response contributes to the high mortality and morbidity of traumatic brain injury (TBI). Potent anti-inflammatory effects can alleviate brain injury after TBI. Fisetin has anti-inflammatory properties in several brain injury models, but the effects of fisetin on inflammation after TBI is still unclear. Our study aimed to investigate the neuroprotective effects of fisetin against inflammation after TBI in mice.Fisetin (25 mg/kg, 50 mg/kg or 75 mg/kg) or equal volume of vehicle was given via intraperitoneal injection 30 min after TBI. The neurological severity score, brain edema and blood brain barrier (BBB) permeability were assayed after TBI. In further mechanistic studies, changes in the toll-like receptor 4 (TLR 4)/nuclear factor-κB (NF-κB) pathway and the expression of pro-inflammatory cytokines were measured. Fisetin significantly improved behavioral outcomes and reduced brain edema after TBI. These changes were associated with significant reductions in TLR 4 expression and NF-κB activity. In addition, changes in the expression of pro-inflammatory cytokines were detected 24 h after TBI. Our study provided the first evidence that fisetin exerted neuroprotective effects by inhibiting the TLR 4/NF-κB–mediated inflammatory pathway after TBI in mice.

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Celecoxib Decrease Seizures Susceptibility in a Rat Model of Inflammation by Inhibiting HMGB1 Translocation

Pharmaceuticals ◽

10.3390/ph14040380 ◽

2021 ◽

Vol 14 (4) ◽

pp. 380

Author(s):

Hadeel Alsaegh ◽

Hala Eweis ◽

Fatemah Kamal ◽

Aziza Alrafiah

Keyword(s):

Oxidative Stress ◽

Inflammatory Cytokines ◽

Control Group ◽

Protective Effects ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Inflammatory Models ◽

Anti Inflammatory ◽

Lung And Kidney ◽

Pro Inflammatory Cytokines

The risk of developing epilepsy is strongly linked to peripheral inflammatory disorders in humans. High-mobility group box protein 1 (HMGB1) has the most focus for being a suspect in this scenario. The current study aimed to detect the celecoxib effect, an anti-inflammatory drug, on decreasing seizure susceptibility and organ damage in lipopolysaccharides (LPS)/pilocarpine (PILO) pretreated Wistar rats. Rats were divided into 6 groups (8 each): group 1 (control), group 2 (PILO), group 3 (PILO+LPS), group 4 (PILO+LPS+(VPA) Valproic acid), group 5 (PILO+LPS+Celecoxib), and group 6 (PILO+LPS+VPA+Celecoxib). LPS was used to induce sepsis and PILO to induce seizures. Oxidative stress markers, pro-inflammatory cytokines, and HMGB1 levels in serum and brain homogenate were evaluated. Histopathological studies were conducted on the hippocampus, liver, lung, and kidney. Treatment with celecoxib either alone or in combination with VPA significantly reduced Racine score and delays latency to generalized tonic-clonic seizures onset with a significant decrease in hippocampal levels of pro-inflammatory cytokines, oxidative stress markers, and increase in reduced glutathione. In addition, celecoxib treatment either alone or in combination with VPA suppressed HMGB1translocation into peripheral circulation more than treatment with VPA alone. Furthermore, hippocampus, liver, lung, and kidney histopathological changes were improved in contrast to other epileptic groups. Celecoxib either alone or combined with VPA has antiepileptic and multiorgan protective effects on acute seizures and inflammatory models induced by PILO with LPS. It decreased histopathological findings, oxidative, and inflammatory effects induced by VPA and LPS. This might be due to its anti-oxidative, anti-inflammatory and anti-HMGB1 mediated effects.

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Immunosuppressant Cyclosporin A-Based Regulation of Lipopolysaccharide-Triggered Pro- and Anti-Inflammatory Cytokines in the Genital Tract of Female Rabbits

10.20944/preprints201811.0289.v1 ◽

2018 ◽

Author(s):

Li-Qin Yang ◽

Qiu-Ying Wu ◽

Xuan-Yu Chen ◽

Chun Wang ◽

Zhang Yan ◽

...

Keyword(s):

Mrna Expression ◽

Inflammatory Cytokines ◽

Genital Tract ◽

Protein Production ◽

Control Group ◽

Protein Levels ◽

Lps Challenge ◽

Anti Inflammatory ◽

Uterine Body ◽

Pro Inflammatory Cytokines

In this study, we evaluated the effects of Cyclosporine A (CsA) on Lipopolysaccharide (LPS)-induced cytokine production in the genital tract of female rabbits. Twelve sexually mature and healthy female rabbits were randomly divided into four groups (n = 3 each). The rabbits in the LPS group were given an intrauterine infusion of Escherichia coli LPS (4 mg/kg body weight (BW)). Rabbits in the CsA group were given CsA (20 mg/kg BW). Rabbits in the LPS + CsA group were given LPS (4 mg/kg BW) and CsA (20 mg/kg BW). The control group received only LPS and CsA carrier. The gene expression and protein levels of pro- and anti-inflammatory cytokines were observed using qRT-PCR and immuno-histochemical (IHC) assay, respectively. Our study showed that IL-1β, IL-6, IL-8, TNF-α, IFN-γ, IL-4, IL-10, IL-13, and TGF-β were expressed in female genital organs. The LPS challenge increased the mRNA expression of IL-6 and TNF-α in the uterine body and IL-1β in the uterotubal junction compared to the control group. CsA increased the basal mRNA expression of anti-inflammatory cytokines (i.e., IL-4 in the uterine body, uterotubal junction, and oviductal ampulla; IL-10 in the cervix, oviductal isthmus, and ampulla; and TGF-β in the uterotubal junction and oviductal ampulla) and pro-inflammatory cytokines (i.e., IL-6 and IL-8 in the cervix; IL-1β in the oviductal isthmus; TNF-α in the oviductal ampulla; and IFN-γ in the uterine body compared to the control group). In addition, CsA inhibited the mRNA expression of pro-inflammatory cytokines, such as IL-6 in the uterine body, uterotubal junction, and oviductal isthmus; TNF-α in the uterine body; and IFN-γ in the uterotubal junction and oviductal isthmus induced by the LPS challenge. The IHC assay showed the LPS-induced increase in protein production of IL-6 in the uterine body and oviductal isthmus. CsA increased the protein production of IL-10 in the cervix, uterine body, oviductal ampulla, and isthmus. Moreover, CsA decreased the protein production of IL-6 in the uterine body and oviductal isthmus induced by LPS.

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Pengaruh Kepatuhan Suplementasi Tablet Kalsium Modifikasi terhadap Kadar Kalsium dan Tekanan Darah Pada Ibu Hamil

PALAPA ◽

10.36088/palapa.v6i1.56 ◽

2018 ◽

Vol 6 (1) ◽

pp. 19-33

Author(s):

Dhany Dahniarti ◽

Irfan Idris ◽

Nasrudin Am

Keyword(s):

Blood Pressure ◽

Pregnant Women ◽

Calcium Level ◽

Intervention Group ◽

Test Group ◽

Maternity Hospital ◽

Control Group ◽

Average Decrease ◽

Level Increase ◽

Pregnant Mothers

A low intake of calcium leads to an increase in high blood pressure by stimulating the release of paratiroid and renin hormones that lead to increase in intracellular calcium concentration in the vasculer smooth muscle cells and result in vasocontriction. This study aims to determine the influence of compliance to supply modified calcium tablet toward calcium level and blood pressure in pregnant mothers. This research was conducted at RSIA Sitti Khadijah I Maternity Hospital Makassar with research design of quasy experiment with prepost test group control design. There were 30 pregnant mother sampels of prehypertension in gestational age> 20 weeks, 12 pregnant women were given normal calcium tablets and 18 pregnant women given modified calcium tablets for 8 weeks each. Data analysis use paired t test. The results indicate that there is a different changes of calcium level and blood pressure between pregnant mothers of intervention group and control group with the value of p = 0.000 (p<0.05). After intervention averagely the calcium level increase in intervention group 0.4 mg/dl while in control group 0.1 mg/dl, followed by the average decrease in blood pressure of systole and dyastole in the intervention group 15.4/9.6 mmHg while in the control group 3.5/4.5 mmHg.

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The pattern of pro- and anti-inflammatory cytokine secretion in patients with secondary edematous breast cancer

Zaporozhye Medical Journal ◽

10.14739/2310-1210.2021.4.214070 ◽

2021 ◽

Vol 23 (4) ◽

pp. 536-540

Author(s):

O. M. Bilyi ◽

N. A. Mitriaieva ◽

M. V. Krasnoselskyi ◽

L. V. Hrebinyk

Keyword(s):

Breast Cancer ◽

Inflammatory Cytokines ◽

Inflammatory Cytokine ◽

Serum Levels ◽

Special Treatment ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Anti Inflammatory ◽

Anti Inflammatory Cytokine ◽

Pro Inflammatory Cytokines

Secondary edematous breast cancer (SEBC), T4b, has a poor prognosis. The aim of this study is to examine the balance in serum levels of pro-inflammatory (TNFά, IL-8) and anti-inflammatory (IL-4) cytokines in patients with SEBC before special treatment. Materials and methods. A total of 87 patients with breast cancer (BC) were examined before treatment: 42 patients with SEBC in T4bN0-3M0 stage and 45 BC patients in T3-4N1-3M0 stage without edema. The control group consisted of 15 patients with fibroadenomas. The serum levels of cytokines (IL-4, IL-8, TNFά) in the patients was determined using the enzyme-linked immunosorbent assay. Results. In the SEBC patients as compared to the patients without cancer, the serum pro-inflammatory cytokine (IL-8, TNFά) levels were significantly increased and the anti-inflammatory cytokine (IL-4) level was slightly increased in 22 %. In BC without edema, an imbalance was noted in favor of pro-inflammatory cytokines, but in SEBC it was more pronounced (31.6 versus 12.4 and 5.6 versus 3.2, respectively). Conclusions. In the majority of SEBC patients, there is an imbalance in the cytokine profile in favor of the pro-inflammatory cytokines (IL-8, TNFά). SEBC patients with elevated levels of both pro- and anti-inflammatory cytokines before treatment are the highest risk group of tumor progression and metastasis. Inhibition of the IL-8 effects or related CXC chemokines, TNFά, and others may have important consequences for the systemic treatment of SEBC.

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