scholarly journals Antioxidant and Antibacterial Activities of Essential Oils from Three Moroccan Species (Lavandula mairei Humbert, Lavandula dentata L. and, Lavandula stoechas L.)

2021 ◽  
pp. 64-71
Author(s):  
Ridwane Ghanimi ◽  
Ahmed Ouhammou ◽  
Yassine El Atki ◽  
Mohamed Cherkaoui
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Klebsiella Pneumoniae ◽  
Essential Oils ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Lavandula Stoechas ◽  
Total Antioxidant

This study aimed to evaluate the antioxidant and the antibacterial activities of the essential oils from three Moroccan species of the genus Lavandula (Lavandula dentata L., Lavandula mairei Humbert and, Lavandula stoechas L.). The antibacterial activities were evaluated by the minimal inhibitory concentration (MIC) and the disc diffusion method, against Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Citrobacter koseri) and Gram-positive bacteria (Staphylococcus aureus). The antioxidant potential was evaluated using free radical Scavenging against 2,2-diphenyl-1-picrylhydrzyl (DPPH), ferric reducing activity power (FRAP), and total antioxidant capacity (TAC). The antibacterial activity of the essential oils showed higher activity against tested nosocomial bacteria especially against Klebsiella pneumoniae, Citrobacter koseri, and Staphylococcus aureus. The essential oil of Lavandula stoechas L. revealed the higher antioxidant activity as measured by DPPH (IC50 = 3,11 ± 0,13 mg/ml), FRAP (EC50 = 6,88 ± 0,8 mg/ml) and TAC (443,2 ± 38 mg AAE/g EO) assays. The essential oil of Moroccan Lavandula stoechas L. could have potential as antimicrobial compounds for the treatment of many infectious bacterial diseases, including multi-resistant bacterial strains of nosocomial origin.

scholarly journals Variability of Chemical Compositions and Antimicrobial and Antioxidant Activities of Ruta chalepensis Leaf Essential Oils from Three Palestinian Regions

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Nidal Jaradat ◽  
Lina Adwan ◽  
Shadi K’aibni ◽  
Abdel Naser Zaid ◽  
Munqez J. Y. Shtaya ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Chemical Compositions ◽  
Ruta Chalepensis

Introduction. Interest in essential oils was recently revived with their popularity increasing in medicine, pharmacy, and aromatherapy. This study was performed to identify the chemical compositions of the essential oil of Ruta chalepensis growing wildly in three regions in Palestine and to assess and compare their antimicrobial and antioxidant activities. Methods. Identification of the essential oil was performed by gas chromatography coupled with mass spectrometry (GC-MS). Antimicrobial activity was tested against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Methicillin-Resistant Staphylococcus aureus, and Candida albicans by using minimum inhibitory concentration (MIC) assay, while antioxidant activity was analyzed by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Results. The essential oils of R. chalepensis from Jerusalem and Hebron regions have almost identical components; the major compounds identified were linalyl acetate and β-linalool; these essential oils exerted potential antioxidant and antibacterial activities. On the other hand, the major components of the plant essential oil from Jenin region were 2-undecanone and 2-nonanone, which exhibited potential antifungal activity. Conclusions. The phytoconstituents and antioxidant and antimicrobial properties of the essential oil of R. chalepensis from different regions in Palestine were established in this study. The obtained results indicate possible applications for R. chalepensis in the treatment of various infectious and noninfectious diseases.

Chemical composition and antibacterial activities of Juniperus horizontalis essential oil

2014 ◽  
Vol 94 (2) ◽  
pp. 323-327 ◽  
Author(s):  
T. Eryiğit ◽  
N. Okut ◽  
K. Ekici ◽  
B. Yildirim
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
The Subject

Eryiğit, T., Okut, N., Ekici, K. and Yildirim, B. 2014. Chemical composition and antibacterial activities of Juniperus horizontalis essential oil. Can. J. Plant Sci. 94: 323–327. In recent years, the screening of antibacterial activity has been the subject of much research, and the antibacterial activity of essential oils could be a promising subject for future investigation. In this study, the antibacterial activities of Juniperus horizontalis essential oils and their components were investigated. Essential oils were isolated using the hydro-distillation method and their components were analyzed by gas chromatography–mass spectrometry (GC-MS). The main compounds found in the oil of leaves were linalool (33.76%), P-cymene (23.20%), gamma-terpinene (8.67%), trans-sabinene hydrate (8.59%), thyme camphor (8.49%), carvol (5.08%) and borneol (4.22%). Juniperus horizontalis essential oils were evaluated for antibacterial activity against six bacterial strains using the disc diffusion method. The results indicate that the essential oil of J. horizontalis shows variable and significant antibacterial activities against the six tested bacteria species. The diameters of the inhibition zones formed for bacteria were measured. The lowest inhibition zone was 12 mm (sensitive) against Pseudomonas aeruginosa ATCC 10145 and the highest zone was 32 mm (extremely sensitive) against Enterococcus faecalis ATCC 29212.

scholarly journals Antibacterial Activities of Essential Oils Extracted from Leaves of Murraya koenigii by Solvent-Free Microwave Extraction and Hydro-Distillation

2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Naciye Erkan ◽  
Zhou Tao ◽  
H. P. Vasantha Rupasinghe ◽  
Burcu Uysal ◽  
Birsen S. Oksal
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Solvent Free ◽  
Microwave Extraction ◽  
Murraya Koenigii ◽  
Inhibition Of Growth ◽  
Germacrene D ◽  
Dpph Radical Scavenging

Solvent-free microwave extraction (SFME) for the isolation of essential oil from leaves of Murraya koenigii L. (Rutaceae) has been compared with the conventional hydro-distilled oil (HD) in terms of yield, composition, antioxidant activity, and antibacterial activity against Listeria innocua. The yield of essential oil obtained from 30 min of SFME was similar to that of HD for 180 min. By GC-MS analysis, the major compounds of the essential oil extracted by SFME, which were obtained in somewhat lower amounts than in the essential oil obtained by HD, were α-copaene (44.3%), β-gurjunene (25.5%), isocaryophyllene (12.1%), β-caryophyllene (8.7%) and germacrene D (2.9%). The content of oxygenated terpenes, slightly higher for the SFME-essential oil (2.3%) than the HD-essential oil (1.4%), were much lower than that of nonoxygenated terpenes in both oils. DPPH radical scavenging activities of both essential oils were relatively low (10%-24%). Complete inhibition of growth of L. innocua was observed with both SFME and HD essential oils, at 400 and 600 μg/mL (minimum inhibitory concentration), respectively. The SFME-essential oil at 300 μg/mL provided 92% inhibition, indicating its potential as a natural antimicrobial agent.

scholarly journals ANTIBACTERIAL ACTIVITY OF SOAP MADE FROM ESSENTIAL OILS AND COMMERCIAL SOAP SOLD IN THE LEBANESE MARKET

2021 ◽  
pp. e304
Author(s):  
Candy GERGES ◽  
Mohamad HADLA ◽  
Michele ELIALI ◽  
Martine RICHA ◽  
Nour Mammari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Effective Measure ◽  
E Coli ◽  
Different Types ◽  
Commercial Soap ◽  
Zones Of Inhibition

Background: Thousands of people are dying as a result of infections caused by bacteria. Among the main routes of germ transmission are the hands, making hand hygiene very important in preventing the spread of pathogens and bacterial infection. Hand washing with soap and water is considered to be a simple and effective measure. Old soap manufacturers have long had traditional uses in the Lebanese community. Methods: The purpose of this study is to compare the antibacterial activity of oil-based soaps with commercial soap sold in the Lebanese market. Different types of herbal soap and antiseptics have been used in this study. Four bacterial strains were used: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli and Enterobacter Spp. The antibacterial activities of these soaps were determined by the diffusion method of disks in agar medium. Results and Discussion: Statistical analysis of zones of inhibition showed that S. epidermidis, P. aeruginosa and Enterobacter Spp. were sensitive only to traditional oil-based soaps, which are Sage, Rose Mary and Cedar. In addition, S. aureus showed sensitivity to soaps comprised of essential oils as well as antibacterial synthetic soap, Dettol and Lifebuoy. On the other hand, E. coli showed resistance to all soaps. Soaps comprised of natural essential oils have shown antibacterial activity superior to so-called “Antibacterial” soaps. Conclusion: Based on this study, we can say that the use of soaps with essential oils might be the best option due to their organic origin as well as their antibacterial proved activities.

scholarly journals Chemical Indices and Antibacterial Properties of Some Essential Oils of Apiaceae and Lauraceae Spices in Southwest of Algeria

2019 ◽  
Author(s):  
Dalila Razni ◽  
Linda Rouisset ◽  
Elhassan Benyagoub
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Antibacterial Properties ◽  
Independent Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Cinnamon Essential Oil ◽  
The One

This study is a part of the valorization of extract from three most commonly used Algerian spices, namely; caraway and cumin seeds and cinnamon bark. On the one hand, it aims at characterizing the chemical indices of extracted essential oils and evaluating the antibacterial activity of each essential oil by titration and disc diffusion method respectively. On the other hand, it attempts at evaluating the combined action of essential oils against four reference pathogenic bacterial strains, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis by well and Chabbert-type diffusion method. The essential oils obtained by the hydrodistillation method have a relatively average extraction about 1.43, 2.3 and 2.5%, respectively for caraway, cumin, and cinnamon. The acid index indicates the behavior and amount of free acids present in the essential oil, in which the acid and saponification indices of cinnamon essential oil indicate a value of 4.48 and 168.56 respectively. It can also inform us about the susceptibility of the oil to undergo alterations. The antibacterial activity results showed that cinnamon essential oil (EO) proved to be the most active against the tested bacterial strains; caraway EO was active against Enterococcus faecalis, and the antibacterial action of cumin EO was the lowest. However, the association of the extracted essential oils has a higher synergistic effect than the independent effect of each essential oil, in which the MIC value found was estimated at 10 to 20 (V/V), 40 to 50 (V/V) and 50 to 70 (V/V) respectively for cinnamon, cumin and caraway. The obtained results show that the response to the antibacterial activity varies according to the plant species used and the extract tested alone or in combination.

scholarly journals Antibacterial of Cinnamon Bark (Cinnamomum burmannii) Essential Oil Against Methicillin-Resistant Staphylococcus aureus

2021 ◽  
Vol 4 (1) ◽  
pp. 56
Author(s):  
Shinta Levea Ni'matul Fadlilah ◽  
Mustofa Helmi Effendi ◽  
Wiwiek Tyasningsih ◽  
Lucia Tri Suwanti ◽  
Jola Rahmahani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Methicillin Resistant ◽  
Cinnamon Bark ◽  
Cinnamomum Burmannii

This study aimed to determine the in vitro antibacterial activities of essential oil from cinnamon bark (Cinnamomum burmannii) on Methicillin-resistant Staphylococcus aureus (MRSA) isolated from raw milk. Essential oil from cinnamon bark obtained from the steam distillation method and essential oil was made in a series dilution with a concentration of 1%, 2%, 4%, and 8%. The antibacterial activities were tested using the disk diffusion method. Results showed from five isolates of MRSA, one isolate was sensitive of essential oil with a concentration 2% and all of the isolate was sensitive with a concentration 4% and 8%. The antibacterial activity showed by inhibition zones on  MRSA. The results suggest that the activity of the essential oils of cinnamon bark has an antibacterial effect on MRSA and it is developed as phytopharmaca.

scholarly journals Escherichia coli and Staphylococcus aureus responsible for bovine mastitis sensitivity to the essential oil of Algerian Thymus fontanesii Boiss. et Reut.

2020 ◽  
Vol 7 (1) ◽  
pp. 26-32
Author(s):  
Bendella Amina nor elhouda ◽  
Ghazi Kheira ◽  
Meliani Samia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Bovine Mastitis ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Zones Of Inhibition

AbstractThe aim of this study is to test two different methods for evaluating the in vitro antibacterial effect of Thymus fontanesii Boiss. et Reut. essential oil against standard and clinical bacterial strains responsible for bovine mastitis: the disc diffusion method or the aromatogram which allows the demonstration of the antibacterial power of essential oils on the bacterial strains tested, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and two strains isolated from bovine mastitis milk S. aureus and E. coli. The inhibition activity of the essential oil of T. fontanesii on bacterial strains by the two methods shows that the antimicrobial power of this oil is very important and is characterized by bactericidal and bacteriostatic action against gram negative and gram positive bacteria. The antimicrobial evaluation by the aromatogram showed good antibacterial activity against all the strains tested, the zones of inhibition of the bacteria were between 23,33±1,527mm and 37,5±3,535 mm. The search for minimum inhibitory concentrations MIC and bactericides CMB made it possible to quantitatively assess the antimicrobial power of this essential oil. In this work, the MIC was 0,625 µl/ml for all strains tested, and the lowest CMB was that of T. fontanesii against E. coli ATCC 25922 was 0,625 µl/ml.

scholarly journals Essential Oils from Fruit and Vegetables, Aromatic Herbs, and Spices: Composition, Antioxidant, and Antimicrobial Activities

Biology ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1091
Author(s):  
Soumi De-Montijo-Prieto ◽  
María del Carmen Razola-Díaz ◽  
Ana María Gómez-Caravaca ◽  
Eduardo Jesús Guerra-Hernandez ◽  
María Jiménez-Valera ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Food Preservation ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Bacterial Strains

In the field of food preservation, encapsulated Essential Oils (EOs) could be the best non-toxic and eco-friendly tool for food preservative applications substituting the chemicals ones that have several disadvantages for the environment and health. Thirteen commercial EOs from plants, fruits, and vegetables were characterized by GC-MS. The antioxidant activity was measured by DPPH and ABTS techniques. Antimicrobial activity was assessed by agar well-diffusion method and the Minimum Inhibitory Concentration (MIC) by agar dilution method against six bacteria, Candida albicans, and Botrytis cinerea. All the EOs tested have demonstrated antioxidant activity in the range of IC50 0.01–105.32 mg/mL. Between them, cinnamon EOs were the best, followed by oregano and thyme EOs. Fennel EO showed the lowest radical scavenging. MIC values ranged from 0.14 to 9 mg/mL. C. cassia, thyme, and oregano EOs were the most effective against the bacterial species tested, and the yeast C. albicans. On the contrary, citric fruit EOs showed low or no inhibition against most bacterial strains. The percentages of inhibition of mycelia growth of B. cinerea ranged from 3.4 to 98.5%. Thyme, oregano, mint, and fennel EOs showed the highest inhibition.

scholarly journals The Antioxidant and Antibacterial Activities of the Essential Oils of Marrubium vulgare Growing Wild in Eastern Algeria

2013 ◽  
Vol 15 ◽  
pp. 159-166 ◽  
Author(s):  
Abderazak Abadi ◽  
Abdellatif Fahima
Keyword(s):  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Marrubium Vulgare ◽  
Eastern Algeria

The chemical composition was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The constituents were identified by their mass spectra and Kovats’ indices. Fivety (50) components in the oil of M. vulgare were identified. The results demonstrated that the major components of the essential oil were: 4,8,12,16-Tetramethyl heptadecan-4-olid (16.97%), Germacrene D-4-ol (9.61%), α- pinéne (9.37%), Phytol (4.87%), Dehydro-sabina ketone (4.12%), Piperitone (3.27%), δ-Cadinene (3.13%), 1-Octen-3-ol (2.35%) and Benzaldehyde (2.31%). The antioxidant activity of the oils was assessed by measurement of metal chelating activity, the reductive potential, the free radical scavenging (DPPH) assay. The antioxidant activity was compared with that of synthetic antioxidants: butylated hydroxyanisole (BHT), and the essential oils. The results showed as the Essential oil of M. vulgare was evaluated for its antibacterial activities against Gram-positive and Gram negative pathogenic bacteria: Listeria monocytogene, Pseudomonas aeruginosa, Agrobacterium tumefaciens and Salmonella enterica.

scholarly journals Antibacterial and Antioxidant activities of Indonesian ginger (jahe emprit) essential oil extracted by hydrodistillation

2019 ◽  
Vol 20 (2) ◽  
pp. 90-96
Author(s):  
Nurul Azizah ◽  
Euis Filaila ◽  
Salahuddin Salahuddin ◽  
Egi Agustian ◽  
Anny Sulaswatty ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Zingiber Officinale ◽  
Food Preservation ◽  
Diffusion Method ◽  
Plant Essential Oils ◽  
Antibacterial Assay ◽  
Free Radical Scavenging Assay

The rhizome of ginger is commonly used as a spice, food, beverage as well as medicine. Plant essential oils including from ginger have been widely used for food preservation, pharmaceutical and alternative medicines.  Currently there are growing interest of consumer for natural sources such as essential oils for natural antibacterial and antioxidant.  Jahe emprit (Zingiber officinale var. Amarum) is one of Indonesian ginger variety used to obtain ginger essential oil. The objective of current study was to investigate the effect of solvent to feed (SF) ratio in hydrodistillation process on yield, chemicals content, antibacterial and antioxidant activities of ginger essential oils from jahe emprit.  SF ratio used in this study is 0.7: 1, 1.7: 1, 2.7: 1.  Chemicals content was conducted using GCMS analysis.  Antibacterial assay was conducted using disc diffusion method against Escherichia coli and Staphylococcus aureus.  Antioxidant assay was conducted using DPPH free radical scavenging assay.  The results show that the highest essential oil yield was obtained from SF ratio 1.7:1 which gave yield of 3.7%.  GCMS analysis shows that camphene was always the major compound present in those 3 SF ratio, although the amount present was differed.  Besides, 4 other major compounds present were varied.  Antibacterial assay using 1% concentration showed ginger oil obtained from SF 0.7:1 and 2.7:1 have the same activities for S. aureus, whereas SF ratio 1.7:1 has the lowest activities.  However for E. coli, all SF ratio gave same results.  For antioxidant activities at 1000 ppm concentration, the highest activity was obtained from SF ratio 2.7:1

Sign in / Sign up

Export Citation Format

Share Document